Biochemical Characterization of a Filtered Synaptoneurosome Preparation from Guinea Pig Cerebral Cortex: Cyclic Adenosine 3’:5’-Monophosphate-generating Systems, Receptors, and Enzymes

A particulate preparation was obtained by low speed centrifugation of guinea pig cerebral cortical homogenates prepared with a Krebs-Henseleit buffer. Light microscopic examination, using a reflected light differential interference contrast system, reveals the presence of intact neurons, axonal fragments, glial cells, and erythrocytes along with an abundance of small spherical entities (diameter about 1.1 μm) and snowman-shaped entities (diameter of larger sphere about 1.1 μm, diameter of attached smaller sphere about 0.6 μm). Many unattached smaller spherical entities are also present (diameter about 0.6 μm). Pressure filtration through 5 or l0-μm Millipore filters, followed by low speed centrifugation and resuspension, removes most of the larger entities to afford a suspension composed mainly of the small spherical and snowman-shaped entities. Electron microscopic examination reveals the presence of many synaptosomes with attached resealed postsynaptic entities. It is proposed that these correspond to the snowman-shaped entities to be termed synaptoneurosomes. Accumulations of cyclic AMP elicited by 2-chloroadenosine and histamine, and by combinations of 2-chloroadenosine, histamine, norepinephrine, and forskolin, are lower in filtered than in unfiltered preparations, whereas accumulations elicited by forskolin are unchanged. Levels of adenylate cyclase are reduced by filtration, whereas levels of phosphodiesterase are unchanged. Filtration reduces levels of markers for whole cells and endothelial cells, whereas neuronal markers such as acetylcholinesterase activity and norepinephrine uptake are increased. Levels of S-100 protein, a marker for glial cells, are not significantly decreased. There is no apparent change in the density of many receptors or ion channels. Levels of A1-adenosine and H1-histamine receptors are increased, whereas levels of so-called peripheral benzodiazepine-binding sites are decreased

Time-dependent rotational stability of dynamic planets with elastic lithospheres

True polar wander (TPW), a reorientation of the rotation axis relative to the solid body, is driven by mass redistribution on the surface or within the planet and is stabilized by two aspects of the planet's viscoelastic response: the delayed viscous readjustment of the rotational bulge and the elastic stresses in the lithosphere. The latter, following Willemann (1984), is known as remnant bulge stabilization. In the absence of a remnant bulge, the rotation of a terrestrial planet is said to be inherently unstable. Theoretical treatments have been developed to treat the final (equilibrium) state in this case and the time-dependent TPW toward this state, including nonlinear approaches that assume slow changes in the inertia tensor. Moreover, remnant bulge stabilization has been incorporated into both equilibrium and linearized, time-dependent treatments of rotational stability. We extend the work of Ricard et al. (1993) to derive a nonlinear, time-dependent theory of TPW that incorporates stabilization by both the remnant bulge and viscous readjustment of the rotational bulge. We illustrate the theory using idealized surface loading scenarios applied to models of both Earth and Mars. We demonstrate that the inclusion of remnant bulge stabilization reduces both the amplitude and timescale of TPW relative to calculations in which this stabilization is omitted. Furthermore, given current estimates of mantle viscosity for both planets, our calculations indicate that departures from the equilibrium orientation of the rotation axis in response to forcings with timescale of 1 Myr or greater are significant for Earth but negligible for Mars

On postglacial sea level—III. Incorporating sediment redistribution

We derive a generalized theory for gravitationally self-consistent, static sea level variations on earth models of arbitrary complexity that takes into account the redistribution of sediments. The theory is an extension of previous work that incorporated, into the governing equations, shoreline migration due to local sea level variations and changes in the geometry of grounded, marine-based ice. In addition, we use viscoelastic Love number theory to present a version of the new theory valid for spherically symmetric earth models. The Love number theory accounts for the gravitational, deformational and rotational effects of the sediment redistribution. As a first, illustrative application of the new theory, we compute the perturbation in sea level driven by an idealized pulse of sediment transport into the Gulf of Mexico. We demonstrate that incorporating a gravitationally self-consistent water load in this case significantly improves the accuracy of sea level predictions relative to previous simplified treatments of the sediment redistribution

Rapid postglacial rebound amplifies global sea level rise following West Antarctic Ice Sheet collapse

Earth_Model_Data is a zipped folder containing the Earth model data for both the standard model (V3D_SD and V3D_RH). A readme file is in this folder. FPRINT_CODE is a zipped folder containing the fingerprint code. A readme file for the code is also in this folder. WAmask_512.gz is a mask for West/East Antarctica, used for masking out changes in East Antarctica. All other files are sea-level outputs for each of the runs on a degree 512 Gauss-Legendre grid (uniform longitudes but unevenly spaced latitudes, as described in the readme for FPRINT_CODE). Files are named SLt_??? and numbered from 0 (elastic response) to 105 (10 ky). They have a 1D layout, with the first line being the time tag in years followed by 512*1024 row entries. A time array is included (tt_v10.dat). More details are in README.tx

Sources of nonlinearities, chatter generation and suppression in metal cutting

The mechanics of chip formation has been revisited in order to understand functional relationships between the process and the technological parameters. This has led to the necessity of considering the chip-formation process as highly nonlinear, with complex interrelations between its dynamics and thermodynamics. In this paper a critical review of the state of the art of modelling and the experimental investigations is outlined with a view to how the nonlinear dynamics perception can help to capture the major phenomena causing instabilities (chatter) in machining operations. The paper is concluded with a case study, where stability of a milling process is investigated in detail, using an analytical model which results in an explicit relation for the stability limit. The model is very practical for the generation of the stability lobe diagrams, which is time consuming when using numerical methods. The extension of the model to the stability analysis of variable pitch cutting tools is also given. The application and verification of the method are demonstrated by several examples

Phosphorus sources for phosphatic Cambrian carbonates

The fossilization of organic remains and shell material by calcium phosphate minerals provides an illuminating, but time-bounded, window into Ediacaran–Cambrian animal evolution. For reasons that remain unknown, phosphatic fossil preservation declined significantly through Cambrian Series 2. Here, we investigate the phosphorus (P) sources for phosphatic Cambrian carbonates, presenting sedimentological, petrographic, and geochemical data from the Cambrian Series 2–3 Thorntonia Limestone, Australia, some of the youngest Cambrian strata to display exceptional phosphatic preservation of small shelly fossils. We find that within Thorntonia sediments, phosphate was remobilized by organic decay and bacterial iron reduction, with subsequent reprecipitation largely as apatite within the interiors of small shelly fossils. We discuss the merits of bioclastic-derived, organic matter–bound, or iron-bound P as potential sources to these strata. Petrographic observations suggest that the dissolution of phosphatic skeletal material did not provide the P for fossil preservation. In contrast, high organic carbon contents imply significant organic fluxes of P to Thorntonia sediments. Sedimentology and iron-speciation data indicate that phosphorus enrichment occurred during times of expanded anoxic, ferruginous conditions in subsurface water masses, suggesting that phosphorus adsorption to iron minerals precipitating from the water column provided a second significant P source to Thorntonia sediments. Simple stoichiometric models suggest that, by themselves, neither organic carbon burial nor an iron shuttle can account for the observed phosphorus enrichment. Thus, we infer that both processes were necessary for the observed phosphorus enrichment and subsequent fossil preservation in the Thorntonia Limestone.Earth and Planetary Science

Snowball Earth climate dynamics and Cryogenian geology-geobiology

Geological evidence indicates that grounded ice sheets reached sea level at all latitudes during two long-lived Cryogenian (58 and ≥5 My) glaciations. Combined uranium-lead and rhenium-osmium dating suggests that the older (Sturtian) glacial onset and both terminations were globally synchronous. Geochemical data imply that CO2 was 102 PAL (present atmospheric level) at the younger termination, consistent with a global ice cover. Sturtian glaciation followed breakup of a tropical supercontinent, and its onset coincided with the equatorial emplacement of a large igneous province. Modeling shows that the small thermal inertia of a globally frozen surface reverses the annual mean tropical atmospheric circulation, producing an equatorial desert and net snow and frost accumulation elsewhere. Oceanic ice thickens, forming a sea glacier that flows gravitationally toward the equator, sustained by the hydrologic cycle and by basal freezing and melting. Tropical ice sheets flow faster as CO2 rises but lose mass and become sensitive to orbital changes. Equatorial dust accumulation engenders supraglacial oligotrophic meltwater ecosystems, favorable for cyanobacteria and certain eukaryotes. Meltwater flushing through cracks enables organic burial and submarine deposition of airborne volcanic ash. The subglacial ocean is turbulent and well mixed, in response to geothermal heating and heat loss through the ice cover, increasing with latitude. Terminal carbonate deposits, unique to Cryogenian glaciations, are products of intense weathering and ocean stratification. Whole-ocean warming and collapsing peripheral bulges allow marine coastal flooding to continue long after ice-sheet disappearance. The evolutionary legacy of Snowball Earth is perceptible in fossils and living organisms

Orbitally forced ice sheet fluctuations during the Marinoan Snowball Earth glaciation

Two global glaciations occurred during the Neoproterozoic. Snowball Earth theory posits that these were terminated after millions of years of frigidity when initial warming from rising atmospheric CO2 concentrations was amplified by the reduction of ice cover and hence a reduction in planetary albedo. This scenario implies that most of the geological record of ice cover was deposited in a brief period of melt-back. However, deposits in low palaeo-latitudes show evidence of glacial–interglacial cycles. Here we analyse the sedimentology and oxygen and sulphur isotopic signatures of Marinoan Snowball glaciation deposits from Svalbard, in the Norwegian High Arctic. The deposits preserve a record of oscillations in glacier extent and hydrologic conditions under uniformly high atmospheric CO2 concentrations. We use simulations from a coupled three-dimensional ice sheet and atmospheric general circulation model to show that such oscillations can be explained by orbital forcing in the late stages of a Snowball glaciation. The simulations suggest that while atmospheric CO2 concentrations were rising, but not yet at the threshold required for complete melt-back, the ice sheets would have been sensitive to orbital forcing. We conclude that a similar dynamic can potentially explain the complex successions observed at other localities

The quantification of COMT mRNA in post mortem cerebellum tissue: diagnosis, genotype, methylation and expression

BACKGROUND: The COMT gene is located on chromosome 22q11, a region strongly implicated in the aetiology of several psychiatric disorders, in particular schizophrenia. Previous research has suggested that activity and expression of COMT is altered in schizophrenia, and is mediated by one or more polymorphisms within the gene, including the functional Val(158)Met polymorphism. METHOD: In this study we examined the expression levels of COMT mRNA using quantitative RT-PCR in 60 post mortem cerebellum samples derived from individuals with schizophrenia, bipolar disorder, depression, and no history of psychopathology. Furthermore, we have examined the methylation status of two CpG sites in the promoter region of the gene. RESULTS: We found no evidence of altered COMT expression or methylation in any of the psychiatric diagnoses examined. We did, however, find evidence to suggest that genotype is related to COMT gene expression, replicating the findings of two previous studies. Specifically, val(158)met (rs165688; Val allele) rs737865 (G allele) and rs165599 (G allele) all showed reduced expression (P < 0.05). Finally, we observe a strong sexual dimorphism in COMT expression, with females exhibiting significantly greater levels of COMT mRNA. CONCLUSION: The expression of COMT does not appear to be altered in the cerebellum of individuals suffering from schizophrenia, bipolar disorder or depression, but does appear to be influenced by single nucleotide polymorphisms within the gene

Late Quaternary sea-level change and early human societies in the central and eastern Mediterranean Basin : an interdisciplinary review

This article reviews key data and debates focused on relative sea-level changes since the Last Interglacial (approximately the last 132,000 years) in the Mediterranean Basin, and their implications for past human populations. Geological and geomorphological landscape studies are critical to archaeology. Coastal regions provide a wide range of resources to the populations that inhabit them. Coastal landscapes are increasingly the focus of scholarly discussions from the earliest exploitation of littoral resources and early hominin cognition, to the inundation of the earliest permanently settled fishing villages and eventually, formative centres of urbanisation. In the Mediterranean, these would become hubs of maritime transportation that gave rise to the roots of modern seaborne trade. As such, this article represents an original review of both the geo-scientific and archaeological data that specifically relate to sea-level changes and resulting impacts on both physical and cultural landscapes from the Palaeolithic until the emergence of the Classical periods. Our review highlights that the interdisciplinary links between coastal archaeology, geomorphology and sea-level changes are important to explain environmental impacts on coastal human societies and human migration. We review geological indicators of sea level and outline how archaeological features are commonly used as proxies for measuring past sea levels, both gradual changes and catastrophic events. We argue that coastal archaeologists should, as a part of their analyses, incorporate important sea-level concepts, such as indicative meaning. The interpretation of the indicative meaning of Roman fishtanks, for example, plays a critical role in reconstructions of late Holocene Mediterranean sea levels. We identify avenues for future work, which include the consideration of glacial isostatic adjustment (GIA) in addition to coastal tectonics to explain vertical movements of coastlines, more research on Palaeolithic island colonisation, broadening of Palaeolithic studies to include materials from the entire coastal landscape and not just coastal resources, a focus on rescue of archaeological sites under threat by coastal change, and expansion of underwater archaeological explorations in combination with submarine geomorphology. This article presents a collaborative synthesis of data, some of which have been collected and analysed by the authors, as the MEDFLOOD (MEDiterranean sea-level change and projection for future FLOODing) community, and highlights key sites, data, concepts and ongoing debates