Adventures in Paragraph Writing: The Development and Refinement of Scalable and Effective Writing Exercises for Large-enrollment Engineering Courses

Adventures in paragraph writing: the development and refinement of scalable and effective writing exercises for large enrollment engineering courses. The ability to communicate effectively is a highly desirable attribute for today’s graduating engineers. Additionally, the inclusion of communication components in technical courses has been shown to enhance learning of technical content and can be leveraged to satisfy non-technical learning outcomes. However, the incorporation of such components in undergraduate engineering curricula remains challenging due to resource limitations, credit hour crunches, and other issues. This paper presents the design considerations and preliminary results from our ongoing work to create an effective, transferrable, low-overhead approach to paragraph writing exercises suitable for inclusion in any large engineering course. Key considerations in the development of these exercises include: identification of the motivations and learning outcomes for each exercise; development and tailoring of writing prompts (questions) appropriate for these outcomes; and the development and implementation of an assessment and feedback strategy,including resource-efficient grading rubrics and techniques.Results are reported from the application of the paragraph writing exercise in a large civil engineering undergraduate fluid mechanics course (120 students; approximately 15 assignments). A primary focus of this first application centered on two key components that must be refined in order for the exercise to be effective and transferrable: (1) the selection of writing prompts, and (2) assessment and feedback. Analysis of student paragraphs highlights the importance of the writing prompts in the success of the exercise, indicating that specific word choice, question focus, and supplemental instruction greatly affected the level of writing students submitted. Some writing prompts were selected to address and enhance technical content in the course, while other writing prompts were developed to broaden student awareness of engineering in societal, environmental, and global contexts. In addition to developing productive writing prompts, the assessment and feedback strategies were evaluated using student surveys and feedback. While minimal marking and holistic rubric assessment methods proved effective from a grading resource standpoint, students were frustrated by the lack of feedback associated with these techniques and uncomfortable with the holistic grading rubric. Data from student surveys point to the importance of giving meaningful feedback to students, and providing them with opportunities to revise their written submissions. Student surveys also highlighted an unforeseen obstacle to the exercise: student resistance to writing in technical courses. We provide several suggestions for overcoming student resistance, as well as improved assessment and feedback strategies that better meet student needs while still not over-burdening instructors and teaching assistants

Frequency-Independent RC Circuit Model for One-Dimensional Carbon Nanostructures

Abstract-We demonstrate that a frequency-independent parallel RC circuit is the simplest model that accurately describes high-frequency electrical conduction in 1-D nanostructures. The resistance is determined from dc measurement, and the capacitance is extracted directly from the measured S-parameters for a ground-signal-ground test structure, without using any fitting parameter. The methodology is applied to carbon nanofibers, and the RC model yields results that are within ±0.5 dB and ±5 • of the measured S-parameters up to 50 GHz. The model is further justified by examining the relationship between S-and Y -parameters of the test network

A communal catalogue reveals Earth's multiscale microbial diversity

Our growing awareness of the microbial world's importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth's microbial diversity.Peer reviewe

A communal catalogue reveals Earth’s multiscale microbial diversity

Our growing awareness of the microbial world’s importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth’s microbial diversity

Frequency-Independent RC Circuit Model for One- Dimensional Carbon Nanostructures

We demonstrate that a frequency-independent parallel RC circuit is the simplest model that accurately describes high-frequency electrical conduction in 1-D nanostructures. The resistance is determined from dc measurement, and the capacitance is extracted directly from the measured S -parameters for a ground-signal-ground test structure, without using any fitting parameter. The methodology is applied to carbon nanofibers, and the RC model yields results that are within ±0.5 dB and ±5° of the measured S -parameters up to 50 GHz. The model is further justified by examining the relationship between S - and Y -parameters of the test network

An 18-Inch Direct Drive Alt-Az Telescope

Abstract A modest aperture alt-az telescope has been designed and is being built for student use at California Polytechnic State University. The telescope's drive system has no gears, belts, or friction wheels; instead direct drive motors and high resolution encoders are completely integrated into the bearing assemblies and telescope superstructure. In altitude, for instance, a ring of permanent magnets is firmly mounted to the OTA while an opposing ring of coils is mounted on the inside of a fork arm. The electronic control system has been designed to operate these brushless motors in a high precision mode. To achieve the highest possible closed loop servo bandwidth, the structure was designed-using finite element analysis as well as traditional tools-to have a very high natural frequency. The direct drive system and stiff structure should effectively counter wind gusts when the telescope is operated out in the open or within a roll-off roof observatory

An 18-Inch Direct Drive Alt-Az Telescope

Abstract A modest aperture alt-az telescope has been designed and is being built for student use at California Polytechnic State University. The telescope's drive system has no gears, belts, or friction wheels; instead direct drive motors and high resolution encoders are completely integrated into the bearing assemblies and telescope superstructure. In altitude, for instance, a ring of permanent magnets is firmly mounted to the OTA while an opposing ring of coils is mounted on the inside of a fork arm. The electronic control system has been designed to operate these brushless motors in a high precision mode. To achieve the highest possible closed loop servo bandwidth, the structure was designed-using finite element analysis as well as traditional tools-to have a very high natural frequency. The direct drive system and stiff structure should effectively counter wind gusts when the telescope is operated out in the open or within a roll-off roof observatory

Allele-Specific Reduction of the Mutant Huntingtin Allele Using Transcription Activator-Like Effectors in Human Huntington's Disease Fibroblasts.

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an abnormal expansion of CAG repeats. Although pathogenesis has been attributed to this polyglutamine expansion, the underlying mechanisms through which the huntingtin protein functions have yet to be elucidated. It has been suggested that postnatal reduction of mutant huntingtin through protein interference or conditional gene knockout could prove to be an effective therapy for patients suffering from HD. For allele-specific targeting, transcription activator-like effectors (TALE) were designed to target single-nucleotide polymorphisms (SNP) in the mutant allele and packaged into a vector backbone containing KRAB to promote transcriptional repression of the disease-associated allele. Additional TALEs were packaged into a vector backbone containing heterodimeric FokI and were designed to be used as nucleases (TALEN) to cause a CAG-collapse in the mutant allele. Human HD fibroblasts were treated with each TALE-SNP or TALEN. Allele-expression was measured using a SNP-genotyping assay and mutant protein aggregation was quantified with Western blots for anti-ubiquitin. The TALE-SNP and TALEN significantly reduced mutant allele expression (p < 0.05) when compared to control transfections while not affecting expression of the nondisease allele. This study demonstrates the potential of allele-specific gene modification using TALE proteins, and provides a foundation for targeted treatment for individuals suffering from Huntington's or other genetically linked diseases

Allele-Specific Reduction of the Mutant Huntingtin Allele Using Transcription Activator-Like Effectors in Human Huntington's Disease Fibroblasts.

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an abnormal expansion of CAG repeats. Although pathogenesis has been attributed to this polyglutamine expansion, the underlying mechanisms through which the huntingtin protein functions have yet to be elucidated. It has been suggested that postnatal reduction of mutant huntingtin through protein interference or conditional gene knockout could prove to be an effective therapy for patients suffering from HD. For allele-specific targeting, transcription activator-like effectors (TALE) were designed to target single-nucleotide polymorphisms (SNP) in the mutant allele and packaged into a vector backbone containing KRAB to promote transcriptional repression of the disease-associated allele. Additional TALEs were packaged into a vector backbone containing heterodimeric FokI and were designed to be used as nucleases (TALEN) to cause a CAG-collapse in the mutant allele. Human HD fibroblasts were treated with each TALE-SNP or TALEN. Allele-expression was measured using a SNP-genotyping assay and mutant protein aggregation was quantified with Western blots for anti-ubiquitin. The TALE-SNP and TALEN significantly reduced mutant allele expression (p < 0.05) when compared to control transfections while not affecting expression of the nondisease allele. This study demonstrates the potential of allele-specific gene modification using TALE proteins, and provides a foundation for targeted treatment for individuals suffering from Huntington's or other genetically linked diseases