Evaluation of the efficacies of selected antibiotics and medicinal plants on common bacterial fish pathogens

An experiment was conducted to compare the efficacies of some selected antibiotics and medicinal plants against common bacterial fish pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Edwardsiella tarda. Four different antibiotics viz., CFCIN (ciprofloxacin), Renamycin (oxytetracycline), DT-10 (doxycicline) and Sulfatrim (sulphadiazine + trimethoprim) were exposed in different doses (100, 75, 50 and 25 ppm) to the culture of freshly isolated bacteria under the in vitro condition for sensitivity test and minimum inhibitory dose (MID) was determined. Based on in vitro results, antibiotics were applied to the experimental infection of Thai silver barb, Barbonymus gonionotus. CFCIN showed the best result with 100% recoveries of challenged fish in prolonged bath treatment. Medicinal plants were selected on the basis of previous studies. Crude extracts were prepared from various parts (leaves and bulb) of garlic, turmeric, akand and neem and four different doses were applied to the fresh culture of pathogenic isolates under the in-vitro condition to determine minimum inhibitory dose (MID). However, garlic offered the best result with 90.00 ± 2.89% recoveries of challenged fish in aquarium trial. Akand + neem, turmeric and akand showed moderate to weak recovery rates with the same dose. The present study thus showed that medicinal plants would be an effective control measure along with antibiotics against bacterial fish diseases

Supercritical fluid extraction of vegetable matrices: Applications, trends and future perspectives of a convincing green technology

Along more than a decade, R\&D on supercritical fluid extraction (SFE) of vegetable matrices has been increasingly reported in the literature. Aiming at portraying the current state of this field and its evolution in terms of raw materials, products, modes of operation, optimization, modeling techniques, and closeness to industrial application, a large compilation of almost 600 essays from 2000 to 2013 has been deeply analyzed in order to unveil those indicators and their trends. Furthermore, strengths and weaknesses are identified, and some remarks that may drive upcoming research are provided. Globally, more than 300 species are reported in the literature, with prevalence of the extraction of seeds (28% of works) and leaves (17%). The main families of extracted compounds, cosolvents and operating conditions adopted are critically examined, being possible to conclude that researchers investigate many times working regions far from the optimum due to practical limitations or absence of experimental optimization. Current phenomenological, statistical and semi-empirical approaches are reviewed, along with scale-up studies, and economic analysis. In the whole, the most comprehensive picture over SFE of vegetable matrices is provided in this review, highlighting pertinent aspects and opportunities that may further consolidate the convincing route of this technology for the next years. (C) 2014 Elsevier B.V. All rights reserved