625 research outputs found
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The Bacterial Carbon-Fixing Organelle Is Formed by Shell Envelopment of Preassembled Cargo
Background: Cyanobacteria play a significant role in the global carbon cycle. In Synechococcuselongatus, the carbon-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is concentrated into polyhedral, proteinaceous compartments called carboxysomes. Methodology/Principal Findings Using live cell fluorescence microscopy, we show that carboxysomes are first detected as small seeds of RuBisCO that colocalize with existing carboxysomes. These seeds contain little or no shell protein, but increase in RuBisCO content over several hours, during which time they are exposed to the solvent. The maturing seed is then enclosed by shell proteins, a rapid process that seals RuBisCO from the cytosol to establish a distinct, solvent-protected microenvironment that is oxidizing relative to the cytosol. These closure events can be spatially and temporally coincident with the appearance of a nascent daughter RuBisCO seed. Conclusions/Significance: Carboxysomes assemble in a stepwise fashion, inside-to-outside, revealing that cargo is the principle organizer of this compartment’s biogenesis. Our observations of the spatial relationship of seeds to previously formed carboxysomes lead us to propose a model for carboxysome replication via sequential fission, polymerization, and encapsulation of their internal cargo
Iowa Crop Variety Yield Testing: A History and Annotated Bibliography
Variety testing by U.S. agricultural universities, often in cooperation with experiment stations, and professional crop associations is recognized as an independent, unbiased validation of the viability of commercial crop varieties. In Iowa, variety testing has also been conducted by many private agricultural companies and individual farmers. Records for crop variety evaluations within the state can be traced back to 1871, well before the creation of the Iowa Agricultural Experiment Station in 1888. The Iowa Corn Yield Test (ICYT) is undeniably the most famous of the Iowa variety yield trials; however, corn (Zea mays L.) varieties were being tested long before that program was initiated. Furthermore, Iowa researchers have been conducting variety yield tests on many other field crops. Knowledge of how Iowa variety tests have been organized and published could be helpful to researchers looking for similar, long-term evaluations from other states and around the world. Variety tests from the past also have the potential to help guide new research efforts and may provide an important untapped resource for unique varietal data. As crop scientists and agronomists look to find new sources for biofuels, bio-products, and other industrial uses for various crops, data from historical varieties could be useful. The objective for this review is to provide an historic account with sections on varietal testing in Iowa. It is presented in chronological order followed by sections devoted to specific crops. A Supplemental Information file containing a detailed annotated bibliography is also provided
Cute Balloons with Thickness
Based on the fnite element method, we present a simple volume-preserved thin shell deformation algorithm to simulate the process of inflating a balloon. Diff erent from other thin shells, the material of balloons has special features: large stretch, small bend and shear, and incompressibility. Previous deformation methods often focus on typical three-dimensional models or thin plate models such as cloth model. The rest thin shell methods are complex or ignore the special features of thin shells especially balloons. We modify the triangle element to simple three-prism element, ignore bending and shearing deformation, and use volume preservation algorithm to match the incompressibility of balloons. Simple gas model is used, which interacts with shells to make the balloons inflated. Di different balloon examples have been tested in our experiments and the results are compared with those of other methods. The experiments show that our algorithm is simple and effective
Human Mycobacterium bovis Infection and Bovine Tuberculosis Outbreak, Michigan, 1994–2007
Mycobacterium bovis is endemic in Michigan’s white-tailed deer and has been circulating since 1994. The strain circulating in deer has remained genotypically consistent and was recently detected in 2 humans. We summarize the investigation of these cases and confirm that recreational exposure to deer is a risk for infection in humans
Validation of the in Vivo Iodo-nitro-tetrazolium (INT) Salt reduction method as a proxy for plankton respiration
Knowledge of the magnitude and variability of plankton respiration is a crucial gap in our understanding of marine carbon cycling. In order to validate the INT reduction method as a proxy for plankton respiration, we have compiled and analysed a dataset (n = 376) of concurrent measurements of dissolved oxygen consumption (CRO2) and in vivo reduction of 2-para (iodophenyl)-3(nitrophenyl)-5(phenyl) tetrazolium chloride tetrazolium salt (INT) spanning a wide range of oceanic regions and physicochemical conditions. Data were randomly divided into two independent subgroups: two thirds of the data were used to derive a regression conversion between dissolved oxygen consumption and INT reduction ("training" dataset) and one third of the data was used to validate the regression ("test" dataset). There was a significant relationship between the log-transformed dissolved oxygen consumption rates and the log-transformed INT reduction rates (INTT) with the "training" dataset (logCRO2 = 0.72logINTT + 0.44, R2 = 0.69, n = 249, p 8 °C and chlorophyll-a concentrations >0.2 μg L-1 and reduced at lower temperatures and chlorophyll-a concentrations. The results of this study endorse the adequate performance and reliability of the INT method for natural plankton communities
Energy-based dissolution simulation using SPH sampling
A novel unified particle-based method is proposed for real-time dissolution simulation that is fast, predictable, independent of sampling resolution, and visually plausible. The dissolution model is derived from collision theory and integrated into a smoothed particle hydrodynamics fluid solver. Dissolution occurs when a solute is submerged in solvent. Physical laws govern the local excitation of solute particles based on kinetic energy: when the local excitation energy exceeds a user-specified threshold (activation energy), the particle will be dislodged from the solid. Solute separation during dissolution is handled using a new Graphics Processing Unit (GPU)-based region growing method. The use of smoothed particle hydrodynamics sampling for both solute and solvent guarantees a predictable and smooth dissolution process and provides user control of the volume change during the phase transition. A mathematical relationship between the activation energy and dissolution time allows for intuitive artistic control over the global dissolution rate. We demonstrate this method using a number of practical examples, including antacid pills dissolving in water, hydraulic erosion of nonhomogeneous terrains, and melting
Osteoblast mineralization requires β1 integrin/ICAP-1–dependent fibronectin deposition
ICAP-1 prevents recruitment of kindlin-2 to β1 integrin to control dynamics of fibrillar adhesion sites, fibronectin deposition, and osteoblast mineralization during bone formation
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Shaping the Future of Research: a perspective from junior scientists
The landscape of scientific research and funding is in flux as a result of tight budgets, evolving models of both publishing and evaluation, and questions about training and workforce stability. As future leaders, junior scientists are uniquely poised to shape the culture and practice of science in response to these challenges. A group of postdocs in the Boston area who are invested in improving the scientific endeavor, planned a symposium held on October 2 nd and 3 rd, 2014, as a way to join the discussion about the future of US biomedical research. Here we present a report of the proceedings of participant-driven workshops and the organizers’ synthesis of the outcomes
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