Listening with Curiosity – Tracking the Acoustic Response of Portable Laser Ablation

Nowadays, one of the methods of choice for minimal invasive sampling of solid matter is laser ablation (LA). Routine LA sampling is performed commonly in the laboratory and the amount of ablated mass can directly be monitored and analysed. By contrast laser-based sampling in the field, using a portable laser ablation system (pLA), still remains challenging concerning low-absorbing or NIR-transparent samples. The current hardware is limited in regards to photon energy and density resulting in unsteady ablation. But as the actual amount of collected mass is the major crux of on-site sampling, with this performance it is often unknown and estimates can only be made based on the experience from prior method development and the experience of the user. In the following work an easy-to-use method to monitor the amount of ablated material collected during laser-based sampling by measuring the acoustic response is presented. The pLA-system was coupled to inductively coupled plasma mass spectrometry (ICPMS) via a diffusion driven gas exchange device (GED) which allowed to monitor mass removal and acoustic response quasi-simultaneously. For the current instrumentation only actual mass removal leads to the formation of shockwaves (SW) and, thus, acoustic signals. These events can be used as indicator for executed LA events and counted on an individual basis. The intensity of acoustic signals has been shown to correlate with the LA mass, i.e., the amount of ablated material. This allows to perform re-adjustment of the laser focus during sampling for optimal ablation based on the intensity of the acoustic signal. Likewise, acoustic intensity together with counting allows the operator to make estimates about total mass sampled. Therefore, unsuccessful laser aerosol collection in the field shall become a thing of the past

A flume experiment to examine underwater sound generation by flowing water

The hydrogeomorphology and ecology of rivers and streams has been subject of intensive research for many decades. However, hydraulically-generated acoustics have been mostly neglected, even though this physical attribute is a robust signal in fluvial ecosystems. Physical generated underwater sound can be used to quantify hydro-geomorphic processes, to differentiate among aquatic habitat types, and it has implications on the behavior of organisms. In this study, acoustic signals were quantified in a flume by varying hydro-geomorphic drivers and the related turbulence and bubble formation. The acoustic signals were recorded using two hydrophones and analyzed using a signal processing software, over 31 third-octave bands (20Hz-20kHz), and then combined in 10 octave bands. The analytical method allowed for a major improvement of the signal-to-noise ratio, therefore greatly reducing the uncertainty in our analyses. Water velocity, relative submergence, and flow obstructions were manipulated in the flume and the resultant acoustic signals recorded. Increasing relative submergence ratio and water velocity were important for reaching a turbulence threshold above which distinct sound levels were generated. Increases in water velocity resulted in increased sound levels over a wide range of frequencies. The increases in sound levels due to relative submergence of obstacles were most pronounced in midrange frequencies (125Hz-2kHz). Flow obstructions in running waters created turbulence and air bubble formation, which again produced specific sound signature

Traumatic abdominal wall hernias: disruptions of the abdominal wall muscles associated to pelvic bone fractures illustrated by two case reports.

Blunt abdominal traumas are often associated with intra-abdominal injuries and pelvic fractures. Traumatic abdominal wall hernias due to disruption of the abdominal wall muscles may be overlooked. Delayed diagnosis can lead to hernia related complications. We present two cases of high kinetic trauma with pelvic fractures and acute traumatic abdominal wall herniation. Both of these cases suffered from a delayed diagnosis and needed surgery to treat the symptomatic herniation. Clinical reassessment and appropriate medical imaging are mandatory in patients with high kinetic abdominal blunt traumas and associated pelvic fracture, in order to prevent delayed diagnosis and possible complications

Effect of waste materials on acoustical properties of semi-dense asphalt mixtures

Among the urban societal burdens rolling noise generation from tire pavement interaction and urban waste stand apart. Many urban waste materials can be used in pavements with comparable mechanical performance. Noise-related pavement characteristics such as porosity, sound absorption and surface texture, were measured for semi-dense low noise pavement mixtures using urban waste materials namely: recycled concrete aggregates, crumb rubber, polyethylene terephthalate and polyethylene. The results show that the use of these materials is a viable sustainable option for low noise pavements, however that may affect the noise reduction properties. With values around 0.2 at 1000 Hz, the sound absorption of all the mixtures is relatively low and the use of mean profile depth (MPD) alone is not enough to characterize the noise reduction properties. Surface texture was altered in different degrees depending on the waste material used. The results presented can aid in policy pertaining to noise abatement and waste reduction

Genetic analysis of rab7 mutants in zebrafish

Vascular network formation requires the fusion of newly formed blood vessels and the emergence of a patent lumen between the newly established connections so that blood flow can start. Lumen formation has been shown to depend on the late endosomal/lysosomal pathway in various organs of animal tubular systems. Here, we identified a late endosomal/lysosomal vesicular fraction (Rab7/Lamp2) in early zebrafish angiogenic sprouts, which appears to contribute to apical membrane growth during lumen formation. To study the effect of the late endocytic pathway on vascular development, we generated mutant alleles for all three rab7 genes in zebrafish ( rab7a, rab7ba, rab7bb ). All rab7 genes are expressed in wild-type zebrafish and we did not detect any compensatory effects by the other rab7 isoforms in single knockout mutants, which were all viable. Only the triple mutant was lethal suggesting some functional redundancy. However, the different rab7 isoforms fulfil also at least partially independent functions because eggs laid from mothers lacking two rab7 ( rab7a and/or rab7bb ). showed reduced survival and contained enlarged yolk granules, suggesting maternal contribution of these two rab7 . Finally, we observed minor effects on lumen formation in embryos which still express one copy of rab7 . Our results support the notion that the late endocytic/lysosomal compartment contributes to lumen expansion

Image‐guided percutaneous cryoablation of unresectable sacrococcygeal chordoma: Feasibility and outcome in a selected group of patients with long term follow‐up

Chordoma is a rare malignant tumor of the axial skeleton. Percutaneous cryoablation (PCA) is a minimally invasive technique that allows freezing of tumors under imaging control. The purpose of our retrospective study was to investigate the outcome of PCA in a selected cohort of patients with sacrococcygeal chordoma, with a minimum of 5 years follow-up. Four patients were treated in 10 sessions. The mean follow-up was 57.3 months. We evaluated the feasibility, the procedure-related complications, the impact on pain control and oncological outcomes. Freezing of 100% of the tumor volume was possible in 60%. Pain control was not reliably evaluable. Local recurrence occurred in 90% of the treated lesions; the mean time to progression was 8.1 months (range 1.5-16). At last follow-up, one patient had died of the disease, one of another cause and one was receiving the best supportive care. The only patient alive without the disease had received additional carbon-ion radiotherapy. The 5-year survival rate after index PCA was 50%. Complete freezing of the tumor was technically challenging, mainly due to the complex local anatomy. Recurrence occurred in 90% of the lesions treated. PCA should be considered with caution in the curative management of sacrococcygeal chordoma

Using natural means to reduce surface transport noise during propagation outdoors

This paper reviews ways of reducing surface transport noise by natural means. The noise abatement solutions of interest can be easily (visually) incorporated in the landscape or help with greening the (sub)urban environment. They include vegetated surfaces (applied to faces or tops of noise walls and on building façades and roofs ), caged piles of stones (gabions), vegetation belts (tree belts, shrub zones and hedges), earth berms and various ways of exploiting ground-surface-related effects. The ideas presented in this overview have been tested in the laboratory and/or numerically evaluated in order to assess or enhance the noise abatement they could provide. Some in-situ experiments are discussed as well. When well-designed, such natural devices have the potential to abate surface transport noise, possibly by complementing and sometimes improving common (non-green) noise reducing devices or measures. Their applicability strongly depends on the available space reserved for the noise abatement and the receiver position

Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements