Structure of coenopopulations and phytocoenotic confinement of Paeonia tenuifolia L. in floristic complexes of Oka-Don lowland

Paeonia tenuifolia L. is a rare and protected plant of Saratov region classified as 2 (V) vulnerable species. It has been established that in Kalininsky district, Saratov region, this plant is characterized by narrow environmental amplitude and is exerted to anthropogenic impacts, the coenopopulations are isolated from each other. Existence of the species in communities of the meadow–steppe floristic complexes of Kalininsky district has confirmed that peony is heliophyte, xeromesophyte, eutrophic plant and calcicole. Photophily of P. tenuifoliais confirmed first of all by its high abundance and occurrence in open steppe communities. In both coenopopulations of P.tenuifolia, the indices of recovery have been higher than 1 (CP 1 Irec = 3.38, CP 2 Irec = 1.67), that is, the coenopopulations are relatively steady, numerous seeds are generated giving birth to viable individuals. The index of replacement showing the ratio of the density of regrowth to total mature fraction of the population has been higher in CP 1 (Irepl = 0.80) and lower in CP 2 (Irepl = 0.67). Moderate index of maturing evidence long-term existence of the species in the generative state

Clinical fnd physiological characteristics of infant born to mothers with chronic arterial hypertension

Research objective: to estimate the condition of newborns from mothers with a chronic arterial hypertensia (AH) in dynamics of early of neonatal adaptations; to estimate between the frequency of development of pathological conditions at newborns and degree of an arterial hypertensia of mother. The clinical analysis is carried out of 48 stories of development and illness of the newborns which mothers were observed and treated during pregnancy concerning a chronic arterial hypertensia of easy or moderate severity level. The standard clinical investigation was carried for children, the somatic and neurologic status was estimated, haemodynamics indicators were supervised, the laboratory (the general and biochemical analysis of blood, CBS) and tool researches (the echocardiogram, EKG, NLS) were spent. Perinatal of the central nervous system, syndrome of maladaptation, hemodynamic disorders and metabolic and hormonal disorders were observed most often at newborns from mothers with hypertension. The exchanges of vodno-elektrolitnogo infringements were revealed at children in observable groups. The pathological conditions of newborns were marked more often at women with high degree of hypertension.Цель исследования: оценить состояние новорожденных от матерей с хронической артериальной гипертензией (АГ) в динамике ранней неонатальной адаптации; оценить зависимости между частотой развития патологических состояний у новорожденных и степенью АГ матери. Проведен клинический анализ 48 историй развития и болезни новорожденных, матери которых наблюдались и лечились во время беременности по поводу хронической артериальной гипертензии легкой или средней степени тяжести. Детям проводилось стандартное клиническое обследование, оценивался соматический и неврологический статус, контролировались показатели гемодинамики, проводились лабораторные (общий и биохимический анализ крови, КОС) и инструментальные исследования (Эхо-КГ, ЭКГ, НСГ). Установлено, что хроническая артериальная гипертензия оказывает неблагоприятное влияние на течение беременности и внутриутробное развитие плода, вызывая хроническую гипоксию плода и снижая адаптационные возможности новорожденного. Наиболее часто у новорожденных от матерей с АГ наблюдались перинатальные поражения центральной нервной системы, синдром дезадаптации, гемодинамические расстройства, а также метаболические и гормональные нарушения. У детей в наблюдаемых группах выявлены нарушения водно-электролитного обмена. Чем выше была степень гипертонии у женщин, тем чаще у новорожденных отмечались патологические состояния перинатального периода

Effect of the antihypertensive therapy used during pregnancy on features of the adaptation period in the infants

The aim of the research: to evaluate the effect of the different variants of the antihypertensive therapy during pregnancy on clinical features of the adaptation period and the state of the metabolic parameters in the infants. The study, including 65 infants of women with chronic hypertension used during pregnancy antihypertensive therapy. Higher anthropometric measures, the low percentage of LUGR, the low incidence of disease, the earlier statements were typical for infants of women who received monotherapy dopegyt during pregnancy. The low anthropometric measures, the high percentage of LUGR, the high incidence of disease, the trend toward more frequent neurological disorders are occurring in the newborns of the mothers who received combination therapy dopegyt and calcium channel blockers. Hemodynamic adaptation proceeded somewhat less favorable in the infants whose mothers received antihypertensive treatment β-blockers.Цель исследования: оценить влияние использования различных вариантов антигипертензивной терапии во время беременности на клинические особенности течения адаптационного периода и состояние некоторых параметров метаболизма у новорожденных. Под наблюдением находились 65 новорожденных от женщин, страдающих хронической артериальной гипертензией и получавших во время беременности антигипертензивную терапию. Для детей от женщин, получавших во время беременности монотерапию допегитом, характерны были высокие антропометрические показатели, низкий процент СЗРП, низкая частота заболеваемости, более ранние сутки выписки. Сниженные антропометрические показатели, высокий процент СЗРП, повышенная заболеваемость, тенденция к более частой неврологической патологии отмечались в группах новорожденных от матерей, находившихся на комбинированной терапии, причем при сочетании допегита с блокаторами кальциевых каналов данные показатели были несколько хуже, чем при сочетании β-адреноблокаторов с блокаторами кальциевых каналов. Гемодинамическая адаптация наименее благоприятно протекала у новорожденных, матери которых получали антигипертензивную терапию β-адреноблокаторами

Биогеохимия мерзлых толщ арктического побережья полуострова Гыдан

Biogeochemistry of permanently frozen deposits on the arctic shore of Gydan peninsula Core drilling was performed in four regions of arctic shore on Gydan peninsula. Complex laboratory analysis of cores allowed to characterize biochemistry of Gydan permafrost. These results are compared with data from other permafrost provinces and are applied for paleoreconstructions.В четырех районах арктического побережья полуострова Гыдан проведено колонковое бурение и отобраны керны мерзлых отложений. Комплекс лабораторных анализов впервые позволил дать биогеохимическую характеристику мерзлых толщ на Гыдане, сравнить полученные результаты с другими районами криолитозоны и применить их для палеореконструкций

The Eurasian Modern Pollen Database (EMPD), version 2

The Eurasian (née European) Modern Pollen Database (EMPD) was established in 2013 to provide a public database of high-quality modern pollen surface samples to help support studies of past climate, land cover, and land use using fossil pollen. The EMPD is part of, and complementary to, the European Pollen Database (EPD) which contains data on fossil pollen found in Late Quaternary sedimentary archives throughout the Eurasian region. The EPD is in turn part of the rapidly growing Neotoma database, which is now the primary home for global palaeoecological data. This paper describes version 2 of the EMPD in which the number of samples held in the database has been increased by 60 % from 4826 to 8134. Much of the improvement in data coverage has come from northern Asia, and the database has consequently been renamed the Eurasian Modern Pollen Database to reflect this geographical enlargement. The EMPD can be viewed online using a dedicated map-based viewer at https://empd2.github.io and downloaded in a variety of file formats at https://doi.pangaea.de/10.1594/PANGAEA.909130 (Chevalier et al., 2019)Swiss National Science Foundation | Ref. 200021_16959

The Eurasian Modern Pollen Database (EMPD), version 2

The Eurasian (nee European) Modern Pollen Database (EMPD) was established in 2013 to provide a public database of high-quality modern pollen surface samples to help support studies of past climate, land cover, and land use using fossil pollen. The EMPD is part of, and complementary to, the European Pollen Database (EPD) which contains data on fossil pollen found in Late Quaternary sedimentary archives throughout the Eurasian region. The EPD is in turn part of the rapidly growing Neotoma database, which is now the primary home for global palaeoecological data. This paper describes version 2 of the EMPD in which the number of samples held in the database has been increased by 60% from 4826 to 8134. Much of the improvement in data coverage has come from northern Asia, and the database has consequently been renamed the Eurasian Modern Pollen Database to reflect this geographical enlargement. The EMPD can be viewed online using a dedicated map-based viewer at https://empd2.github.io and downloaded in a variety of file formats at https://doi.pangaea.de/10.1594/PANGAEA.909130 (Chevalier et al., 2019).Peer reviewe

Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells

Background Glioblastoma (GBM) is the most common primary brain malignancy and confers a dismal prognosis. GBMs harbor glioblastoma-initiating cells (GICs) that drive tumorigenesis and contribute to therapeutic resistance and tumor recurrence. Consequently, there is a strong rationale to target this cell population in order to develop new molecular therapies against GBM. Accumulating evidence indicates that Nα-terminal acetyltransferases (NATs), that are dysregulated in numerous human cancers, can serve as therapeutic targets. Methods Microarrays were used to study the expression of several NATs including NAT12/NAA30 in clinical samples and stem cell cultures. The expression of NAT12/NAA30 was analyzed using qPCR, immunolabeling and western blot. We conducted shRNA-mediated knockdown of NAT12/NAA30 gene in GICs and studied the effects on cell viability, sphere-formation and hypoxia sensitivity. Intracranial transplantation to SCID mice enabled us to investigate the effects of NAT12/NAA30 depletion in vivo. Using microarrays we identified genes and biochemical pathways whose expression was altered upon NAT12/NAA30 down-regulation. Results While decreased expression of the distal 3’UTR of NAT12/NAA30 was generally observed in GICs and GBMs, this gene was strongly up-regulated at the protein level in GBM and GICs. The increased protein levels were not caused by increased levels of the steady state mRNA but rather by other mechanisms. Also, shorter 3’UTR of NAT12/NAA30 correlated with poor survival in glioma patients. As well, we observed previously not described nuclear localization of this typically cytoplasmic protein. When compared to non-silencing controls, cells featuring NAT12/NAA30 knockdown exhibited reduced cell viability, sphere-forming ability, and mitochondrial hypoxia tolerance. Intracranial transplantation showed that knockdown of NAT12/NAA30 resulted in prolonged animal survival. Microarray analysis of the knockdown cultures showed reduced levels of HIF1α and altered expression of several other genes involved in the hypoxia response. Furthermore, NAT12/NAA30 knockdown correlated with expressional dysregulation of genes involved in the p53 pathway, ribosomal assembly and cell proliferation. Western blot analysis revealed reduction of HIF1α, phospho-MTOR(Ser2448) and higher levels of p53 and GFAP in these cultures. Conclusion NAT12/NAA30 plays an important role in growth and survival of GICs possibly by regulating hypoxia response (HIF1α), levels of p-MTOR (Ser2448) and the p53 pathway

Differences in Caco-2 cell attachment, migration on collagen and fibronectin coated polyelectrolyte surfaces

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