Probing the Earth's interior with a large-volume liquid scintillator detector

A future large-volume liquid scintillator detector would provide a high-statistics measurement of terrestrial antineutrinos originating from $\beta$-decays of the uranium and thorium chains. In addition, the forward displacement of the neutron in the detection reaction $\bar\nu_e+p\to n+e^+$ provides directional information. We investigate the requirements on such detectors to distinguish between certain geophysical models on the basis of the angular dependence of the geoneutrino flux. Our analysis is based on a Monte-Carlo simulation with different levels of light yield, considering both unloaded and gadolinium-loaded scintillators. We find that a 50 kt detector such as the proposed LENA (Low Energy Neutrino Astronomy) will detect deviations from isotropy of the geoneutrino flux significantly. However, with an unloaded scintillator the time needed for a useful discrimination between different geophysical models is too large if one uses the directional information alone. A Gd-loaded scintillator improves the situation considerably, although a 50 kt detector would still need several decades to distinguish between a geophysical reference model and one with a large neutrino source in the Earth's core. However, a high-statistics measurement of the total geoneutrino flux and its spectrum still provides an extremely useful glance at the Earth's interior.Comment: 21 pages, 9 figures. Minor changes, version accepted for publication in Astroparticle Physic

Search for modulations of the solar Be-7 flux in the next-generation neutrino observatory LENA

A next-generation liquid-scintillator detector will be able to perform high-statistics measurements of the solar neutrino flux. In LENA, solar Be-7 neutrinos are expected to cause 1.7x10^4 electron recoil events per day in a fiducial volume of 35 kilotons. Based on this signal, a search for periodic modulations on sub-percent level can be conducted, surpassing the sensitivity of current detectors by at least a factor of 20. The range of accessible periods reaches from several minutes, corresponding to modulations induced by helioseismic g-modes, to tens of years, allowing to study long-term changes in solar fusion rates.Comment: 15 pages, 9 figure

Mellin moments of spin dependent and independent PDFs of the pion and rho meson

We compute the second moments of pion and rho parton distribution functions in lattice QCD with Nf=2+1 flavors of improved Wilson fermions. We determine both singlet and nonsinglet flavor combinations and, for the first time, take disconnected contributions fully into account. In the case of the rho, we also calculate the additional contribution arising from the b1 structure function. The numerical analysis includes 26 ensembles, mainly generated by the CLS effort, with pion masses ranging from 420 down to 214 MeV and with 5 different lattice spacings in the range of 0.1 to 0.05 fm. This enables us to take the continuum limit, as well as to resolve the quark mass dependencies reliably. Additionally we discuss the contaminations of rho correlation functions by two-pion states

Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g

Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized a mitochondrial membrane protein in yeast, Fcj1 (formation of CJ protein 1), which is specifically enriched in CJs. Cells lacking Fcj1 lack CJs, exhibit concentric stacks of inner membrane in the mitochondrial matrix, and show increased levels of F1FO–ATP synthase (F1FO) supercomplexes. Overexpression of Fcj1 leads to increased CJ formation, branching of cristae, enlargement of CJ diameter, and reduced levels of F1FO supercomplexes. Impairment of F1FO oligomer formation by deletion of its subunits e/g (Su e/g) causes CJ diameter enlargement and reduction of cristae tip numbers and promotes cristae branching. Fcj1 and Su e/g genetically interact. We propose a model in which the antagonism between Fcj1 and Su e/g locally modulates the F1FO oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristae tips

Coeliac disease is associated with depression in children and young adults with type 1 diabetes: results from a multicentre diabetes registry

Aims To analyse the association between coeliac disease (CD) and depression in children, adolescents, and young adults with type 1 diabetes (T1D). Methods We included 79,067 T1D patients aged 6-20 years, with at least six months of diabetes duration, and treatment data between 1995 and 2019 were documented in the diabetes patient follow-up registry. We categorized patients into four groups: T1D only (n = 73,699), T1 + CD (n = 3379), T1D + depression (n = 1877), or T1D + CD + depression (n = 112). Results CD and depression were significantly associated (adjusted OR: 1.25 [1.03-1.53]). Females were more frequent in both the depression and the CD group compared with the T1D only group. Insulin pumps were used more frequently in T1D + CD and T1D + depression compared with T1D only (both p < .001). HbA1c was higher in T1D + depression (9.0% [8.9-9.0]), T1D + CD + depression (8.9% [8.6-9.2]), both compared with T1D only (8.2% [8.2-8.2], all p < .001). We found comorbid autism, attention deficit hyperactivity disorder, anxiety, schizophrenia, and eating disorders more frequently in the T1D + CD + depression group compared with T1D only (all p < .001). Conclusions CD and depression are associated in young T1D patients. The double load of T1D and CD may lead to an increased risk for depression. Depression was associated with additional psychological and neurological comorbidities. Aside from imperative CD screening after T1D diagnosis and regular intervals, depression screening might be helpful in routine care, especially in patients with diagnosed CD

An improved method for measuring muon energy using the truncated mean of dE/dx

The measurement of muon energy is critical for many analyses in large Cherenkov detectors, particularly those that involve separating extraterrestrial neutrinos from the atmospheric neutrino background. Muon energy has traditionally been determined by measuring the specific energy loss (dE/dx) along the muon's path and relating the dE/dx to the muon energy. Because high-energy muons (E_mu > 1 TeV) lose energy randomly, the spread in dE/dx values is quite large, leading to a typical energy resolution of 0.29 in log10(E_mu) for a muon observed over a 1 km path length in the IceCube detector. In this paper, we present an improved method that uses a truncated mean and other techniques to determine the muon energy. The muon track is divided into separate segments with individual dE/dx values. The elimination of segments with the highest dE/dx results in an overall dE/dx that is more closely correlated to the muon energy. This method results in an energy resolution of 0.22 in log10(E_mu), which gives a 26% improvement. This technique is applicable to any large water or ice detector and potentially to large scintillator or liquid argon detectors.Comment: 12 pages, 16 figure

Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Quality in CHO Cells

10.1371/journal.pone.0063247PLoS ONE85

A quantitative proteomic analysis of cellular responses to high glucose media in Chinese hamster ovary cells

A goal in recombinant protein production using Chinese hamster ovary (CHO) cells is to achieve both high specific productivity and high cell density. Addition of glucose to the culture media is necessary to maintain both cell growth and viability. We varied the glucose concentration in the media from 5 to 16 g/L and found that although specific productivity of CHO-DG44 cells increased with the glucose level, the integrated viable cell density decreased. To examine the biological basis of these results, we conducted a discovery proteomic study of CHO-DG44 cells grown under batch conditions in normal (5 g/L) or high (15 g/L) glucose over 3, 6, and 9 days. Approximately 5,000 proteins were confidently identified against an mRNA-based CHO-DG44 specific proteome database, with 2,800 proteins quantified with at least two peptides. A self-organizing map algorithm was used to deconvolute temporal expression profiles of quantitated proteins. Functional analysis of altered proteins suggested that differences in growth between the two glucose levels resulted from changes in crosstalk between glucose metabolism, recombinant protein expression, and cell death, providing an overall picture of the responses to high glucose environment. The high glucose environment may enhance recombinant dihydrofolate reductase in CHO cells by up-regulating NCK1 and down-regulating PRKRA, and may lower integrated viable cell density by activating mitochondrial- and endoplasmic reticulum-mediated cell death pathways by up-regulating HtrA2 and calpains. These proteins are suggested as potential targets for bioengineering to enhance recombinant protein production

Uniform nomenclature for the mitochondrial contact site and cristae organizing system

The mitochondrial inner membrane contains a large protein complex that functions in inner membrane organization and formation of membrane contact sites. The complex was variably named the mitochondrial contact site complex, mitochondrial inner membrane organizing system, mitochondrial organizing structure, or Mitofilin/Fcj1 complex. To facilitate future studies, we propose to unify the nomenclature and term the complex "mitochondrial contact site and cristae organizing system" and its subunits Mic10 to Mic60