The worm has turned: Behavioural drivers of reproductive isolation between cryptic lineages

AbstractBehavioural processes such as species recognition and mate attraction signals enforce and reinforce the reproductive isolation required for speciation. The earthworm Lumbricus rubellus in the UK is deeply differentiated into two major genetic lineages, ‘A’ and ‘B’. These are often sympatric at certain sites, but it is not known whether they are to some extent reproductively isolated. Behavioural tests were performed, in which individually genotyped worms were able to choose between soils previously worked either by genetically similar or dissimilar individuals (N = 45). We found that individuals (75%) were significantly (P < 0.05) more likely to orientate towards the soil conditioned by worms of their own lineage. Further testing involved a choice design based on filter papers wetted with water extracts of soils worked by a different genotype on each side (N = 18) or extracts from worked soil vs. un-worked control soil (N = 10). Again, earthworms orientated towards the extract from their kindred genotype (P < 0.05). These findings indicate that genotype-specific water-soluble chemicals are released by L. rubellus; furthermore, they are behaviour-modifying, and play a role in reproductive isolation between sympatric earthworm lineages of cryptic sibling species, through pre-copulatory assortative mate choice

Progress on a gas-accepting ion source for continuous-flow accelerator mass spectrometry

Author Posting. © Elsevier B.V., 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 259 (2007): 83-87, doi:10.1016/j.nimb.2007.01.189.A gas-accepting microwave-plasma ion source is being developed for continuous-flow Accelerator Mass Spectrometry (AMS). Characteristics of the ion source will be presented. Schemes for connecting a gas or liquid chromatograph to the ion source will also be discussed

Detailed electronic structure studies on superconducting MgB2_2 and related compounds

In order to understand the unexpected superconducting behavior of MgB$_2$ compound we have made electronic structure calculations for MgB$_2$ and closely related systems. Our calculated Debye temperature from the elastic properties indicate that the average phonon frequency is very large in MgB$_2$ compared with other superconducting intermetallics and the exceptionally high $T_c$ in this material can be explained through BCS mechanism only if phonon softening occurs or the phonon modes are highly anisotropic. We identified a doubly-degenerate quasi-two dimensional key-energy band in the vicinity of $E_{F}$ along $\Gamma$-A direction of BZ which play an important role in deciding the superconducting behavior of this material. Based on this result, we have searched for similar kinds of electronic feature in a series of isoelectronic compounds such as BeB$_2$, CaB$_2$, SrB$_2$, LiBC and MgB$_2$C$_2$ and found that MgB$_2$C$_2$ is one potential material from the superconductivity point of view. There are contradictory experimental results regarding the anisotropy in the elastic properties of MgB$_2$ ranging from isotropic, moderately anisotropic to highly anisotropic. In order to settle this issue we have calculated the single crystal elastic constants for MgB$_2$ by the accurate full-potential method and derived the directional dependent linear compressibility, Young's modulus, shear modulus and relevant elastic properties. We have observed large anisotropy in the elastic properties. Our calculated polarized optical dielectric tensor shows highly anisotropic behavior even though it possesses isotropic transport property. MgB$_2$ possesses a mixed bonding character and this has been verified from density of states, charge density and crystal orbital Hamiltonian population analyses

The black-hole masses of Seyfert galaxies and quasars

The central black-hole masses of a sample of 30 luminous quasars are estimated using H-beta FWHM measurements from a combination of new and previously-published nuclear spectra. The quasar black-hole mass estimates are combined with reverberation-mapping measurements for a sample of Seyfert galaxies (Wandel 1999) in order to study AGN black-hole masses over a wide range in nuclear luminosity. It is found that black-hole mass and bulge luminosity are well correlated and follow a relation consistent with that expected if black-hole and bulge mass are directly proportional. Contrary to the results of Wandel (1999) no evidence is found that Seyfert galaxies follow a different Mblack-Mbulge relation to quasars. However, the black-hole mass distributions of the radio-loud and radio-quiet quasar sub-samples are found to be significantly different, with the median black-hole mass of the radio-loud quasars a factor of three larger than their radio-quiet counterparts. Finally, utilizing the elliptical galaxy fundamental plane to provide stellar velocity dispersion estimates, a comparison is performed between the virial H-beta black-hole mass estimates and those of the Mblack-sigma correlations of Gebhardt et al. (2000a) and Merritt & Ferrarese (2000). With the disc-like geometry of the broad-line region adopted in this paper, the virial H-beta black-hole masses indicate that the correct normalization of the black-hole vs. bulge mass relation is Mblack=0.0025Mbulge, while the standard assumption of purely random broad-line velocities leads to Mblack=0.0008Mbulge. The normalization of Mblack=0.0025Mbulge provided by the disc model is in remarkably good agreement with that inferred for our quasar sample using the (completely independent) Mblack-sigma correlations.Comment: 10 pages, 6 figures, accepted for publication in MNRA

Photon-photon absorption above a molecular cloud torus in blazars

Gamma rays have been observed from two blazars at TeV energies. One of these, Markarian 421, has been observed also at GeV energies and has roughly equal luminosity per decade at GeV and TeV energies. Photon-photon pair production on the infrared background radiation is expected to prevent observation above about 1 TeV. However, the infrared background is not well known and it may be possible to observe the nearest blazars up to energies somewhat below about 100 TeV where absorption on the cosmic microwave background will give a sharp cut-off. Blazars are commonly believed to correspond to low power radio galaxies, seen down along a relativistic jet; as such they are all expected to have the nuclear activity encircled by a dusty molecular torus, which subtends an angle of 90 degrees or more in width as seen from the central source. Photon-photon pair production can also take place on the infrared radiation produced at the AGN by this molecular torus and surrounding outer disk. We calculate the optical depth for escaping gamma-rays produced near the central black hole and at various points along the jet axis for the case of blazars where the radiation is observed in a direction closely aligned with the jet. We find that the TeV emission site must be well above the top of the torus. For example, if the torus has an inner radius of 0.1 pc and an outer radius of 0.2 pc, then the emission site in Mrk 421 would have be at least 0.25 pc above the upper surface of the torus, and if Mrk 421 is observed above 50 TeV in the future, the emission site would have to be at least 0.5 pc above the upper surface. This has important implications for models of gamma-ray emission in active galactic nuclei.Comment: 18 pages, 6 figures, accepted for publication in Astroparticle Physics. Revised version contains 2 additional figures and more detailed discussio

Impact of Chlamydia trachomatis in the reproductive setting: British Fertility Society Guidelines for practice

Chlamydia trachomatis infection of the genital tract is the most common sexually transmitted infection and has a world-wide distribution. The consequences of infection have an adverse effect on the reproductive health of women and are a common cause of infertility. Recent evidence also suggests an adverse effect on male reproduction. There is a need to standardise the approach in managing the impact of C. trachomatis infection on reproductive health. We have surveyed current UK practice towards screening and management of Chlamydia infections in the fertility setting. We found that at least 90% of clinicians surveyed offered screening. The literature on this topic was examined and revealed a paucity of solid evidence for estimating the risks of long-term reproductive sequelae following lower genital tract infection with C. trachomatis. The mechanism for the damage that occurs after Chlamydial infections is uncertain. However, instrumentation of the uterus in women with C. trachomatis infection is associated with a high risk of pelvic inflammatory disease, which can be prevented by appropriate antibiotic treatment and may prevent infected women from being at increased risk of the adverse sequelae, such as ectopic pregnancy and tubal factor infertility. Recommendations for practice have been proposed and the need for further studies is identified

Feint Lines: Notes on the Creation of a Skateboard Choreography

Magnetic fields on a range of scales play a large role in the ecosystems of galaxies, both in the galactic disk and in the extended layers of gas away from the plane. Observing magnetic field strength, structure and orientation is complex, and necessarily indirect. Observational data of magnetic fields in the halo of the Milky Way are scarce, and non-conclusive about the large-scale structure of the field. In external galaxies, various large-scale configurations of magnetic fields are measured, but many uncertainties about exact configurations and their origin remain. There is a strong interaction between magnetic fields and other components in the interstellar medium such as ionized and neutral gas and cosmic rays. The energy densities of these components are comparable on large scales, indicating that magnetic fields are not passive tracers but that magnetic field feedback on the other interstellar medium components needs to be taken into account.Comment: 13 pages, 7 figures. Accepted in Space Science Review

Formulation, stabilisation and encapsulation of bacteriophage for phage therapy

Against a backdrop of global antibiotic resistance and increasing awareness of the importance of the human microbiota, there has been resurgent interest in the potential use of bacteriophages for therapeutic purposes, known as phage therapy. A number of phage therapy phase I and II clinical trials have concluded, and shown phages don’t present significant adverse safety concerns. These clinical trials used simple phage suspensions without any formulation and phage stability was of secondary concern. Phages have a limited stability in solution, and undergo a significant drop in phage titre during processing and storage which is unacceptable if phages are to become regulated pharmaceuticals, where stable dosage and well defined pharmacokinetics and pharmacodynamics are de rigueur. Animal studies have shown that the efficacy of phage therapy outcomes depend on the phage concentration (i.e. the dose) delivered at the site of infection, and their ability to target and kill bacteria, arresting bacterial growth and clearing the infection. In addition, in vitro and animal studies have shown the importance of using phage cocktails rather than single phage preparations to achieve better therapy outcomes. The in vivo reduction of phage concentration due to interactions with host antibodies or other clearance mechanisms may necessitate repeated dosing of phages, or sustained release approaches. Modelling of phage-bacterium population dynamics reinforces these points. Surprisingly little attention has been devoted to the effect of formulation on phage therapy outcomes, given the need for phage cocktails, where each phage within a cocktail may require significantly different formulation to retain a high enough infective dose. This review firstly looks at the clinical needs and challenges (informed through a review of key animal studies evaluating phage therapy) associated with treatment of acute and chronic infections and the drivers for phage encapsulation. An important driver for formulation and encapsulation is shelf life and storage of phage to ensure reproducible dosages. Other drivers include formulation of phage for encapsulation in micro- and nanoparticles for effective delivery, encapsulation in stimuli responsive systems for triggered controlled or sustained release at the targeted site of infection. Encapsulation of phage (e.g. in liposomes) may also be used to increase the circulation time of phage for treating systemic infections, for prophylactic treatment or to treat intracellular infections. We then proceed to document approaches used in the published literature on the formulation and stabilisation of phage for storage and encapsulation of bacteriophage in micro- and nanostructured materials using freeze drying (lyophilization), spray drying, in emulsions e.g. ointments, polymeric microparticles, nanoparticles and liposomes. As phage therapy moves forward towards Phase III clinical trials, the review concludes by looking at promising new approaches for micro- and nanoencapsulation of phages and how these may address gaps in the field

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2,3,4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease