The use of fluorescent probes to characterize conformational changes in the interaction between vitronectin and plasminogen activator inhibitor-1

Plasminogen activator inhibitor-1 (PAI-1), the primary inhibitor of tissue-type plasminogen activator and urokinase, is known to convert readily to a latent form by insertion of the reactive center loop into a central β- sheet. Interaction with vitronectin stabilizes PAI-1 and decreases the rate of conversion to the latent form, but conformational effects of vitronectin on the reactive center loop of PAI-1 have not been documented. Mutant forms of PAI-1 were designed with a cysteine substitution at either position P1\u27 or P9 of the reactive center loop. Labeling of the unique cysteine with a sulfhydryl-reactive fluorophore provides a probe that is sensitive to vitronectin binding. Results indicate that the scissile P1-P1\u27 bond of PAI-1 is more solvent exposed upon interaction with vitronectin, whereas the N- terminal portion of the reactive loop does not experience a significant change in its environment. These results were complemented by labeling vitronectin with an arginine-specific coumarin probe which compromises heparin binding but does not interfere with PAI-1 binding to the protein. Dissociation constants of approximately 100 nM are calculated for the vitronectin/PAI-1 interaction from titrations using both fluorescent probes. Furthermore, experiments in which PAI-1 failed to compete with heparin for binding to vitronectin argue for separate binding sites for the two ligands on vitronectin

An extreme [OIII] emitter at z=3.2z=3.2: a low metallicity Lyman continuum source

[Abridged] We investigate the physical properties of a Lyman continuum emitter candidate at $z=3.212$ with photometric coverage from $U$ to MIPS 24$\mu$m band and VIMOS/VLT and MOSFIRE/Keck spectroscopy. Investigation of the UV spectrum confirms a direct spectroscopic detection of the Lyman continuum emission with $S/N>5$. Non-zero Ly$\alpha$ flux at the systemic redshift and high Lyman-$\alpha$ escape fraction suggest a low HI column density. The weak C and Si low-ionization absorption lines are also consistent with a low covering fraction along the line of sight. The [OIII]$\lambda\lambda4959,5007+\mathrm{H}\beta$ equivalent width is one of the largest reported for a galaxy at $z>3$ ($\mathrm{EW}([\mathrm{OIII}]\lambda\lambda4959,5007+\mathrm{H}\beta) \simeq 1600\AA$, rest-frame) and the NIR spectrum shows that this is mainly due to an extremely strong [OIII] emission. The large observed [OIII]/[OII] ratio ($>10$) and high ionization parameter are consistent with prediction from photoionization models in case of a density-bounded nebula scenario. Furthermore, the $\mathrm{EW}([\mathrm{OIII}]\lambda\lambda4959,5007+\mathrm{H}\beta)$ is comparable to recent measurements reported at $z\sim7-9$, in the reionization epoch. We also investigate the possibility of an AGN contribution to explain the ionizing emission but most of the AGN identification diagnostics suggest that stellar emission dominates instead. This source is currently the first high-$z$ example of a Lyman continuum emitter exhibiting indirect and direct evidences of a Lyman continuum leakage and having physical properties consistent with theoretical expectation from Lyman continuum emission from a density-bounded nebula.Comment: 14 pages, 11 figures, accepted for publication in A&A. Minor modifications, Figure 2 updated, Figure 9 adde

The properties of the brightest Lyα emitters at z ∼ 5.7

We use deep Very Large Telescope (VLT) optical and near-infrared spectroscopy and deep Spitzer/IRAC imaging to examine the properties of two of the most luminous Lyα emitters at z= 5.7. The continuum redward of the Lyα line is clearly detected in both objects, thus facilitating a relatively accurate measurement (10-20 per cent uncertainties) of the observed rest-frame equivalent widths, which are around 160 Å for both objects. Through detailed modelling of the profile of the Lyα line with a 3D Monte Carlo radiative transfer code, we estimate the intrinsic rest-frame equivalent width of Lyα and find values that are around 300 Å, which is at the upper end of the range allowed for very young, moderately metal-poor star-forming galaxies. However, the uncertainties are large and values as high as 700 Å are permitted by the data. Both Lyα emitters are detected at 3.6 m in deep images taken with the Spitzer Space Telescope. We use these measurements, the measurement of the continuum redward of Lyα and other photometry to constrain the spectral energy distributions of these very luminous Lyα emitters and to compare them with three similar Lyα emitters from the literature. The contribution from nebular emission is included in our models: excluding it results in significantly higher masses. Four of the five Lyα emitters have masses of the order of ∼109 M⊙ and fairly high specific star formation rates (≳10-100 Gyr−1). While our two Lyα emitters appear similar in terms of the observed Lyα rest-frame equivalent width, they are quite distinct from each other in terms of age, mass and star formation history. Evidence for dust is found in all objects, and emission from nebular lines often makes a dominant contribution to the rest-frame 3.6 m flux. Rich in emission lines, these objects are prime targets for the next generation of extremely large telescopes, the James Webb Space Telescope (JWST) and the Atacama Large Millimeter Array (ALMA

The Lyman Alpha Reference Sample: V. The impact of neutral ISM kinematics and geometry on Lyman Alpha escape

We present high-resolution far-UV spectroscopy of the 14 galaxies of the Lyman Alpha Reference Sample; a sample of strongly star-forming galaxies at low redshifts ($0.028 < z < 0.18$). We compare the derived properties to global properties derived from multi band imaging and 21 cm HI interferometry and single dish observations, as well as archival optical SDSS spectra. Besides the Lyman $\alpha$ line, the spectra contain a number of metal absorption features allowing us to probe the kinematics of the neutral ISM and evaluate the optical depth and and covering fraction of the neutral medium as a function of line-of-sight velocity. Furthermore, we show how this, in combination with precise determination of systemic velocity and good Ly$\alpha$ spectra, can be used to distinguish a model in which separate clumps together fully cover the background source, from the "picket fence" model named by Heckman et al. (2011). We find that no one single effect dominates in governing Ly$\alpha$ radiative transfer and escape. Ly$\alpha$ escape in our sample coincides with a maximum velocity-binned covering fraction of $\lesssim 0.9$ and bulk outflow velocities of $\gtrsim 50$ km s$^{-1}$, although a number of galaxies show these characteristics and yet little or no Ly$\alpha$ escape. We find that Ly$\alpha$ peak velocities, where available, are not consistent with a strong backscattered component, but rather with a simpler model of an intrinsic emission line overlaid by a blueshifted absorption profile from the outflowing wind. Finally, we find a strong anticorrelation between H$\alpha$ equivalent width and maximum velocity-binned covering factor, and propose a heuristic explanatory model.Comment: 28 pages, 19 figures, 5 table

The Lyman alpha Reference Sample: Extended Lyman alpha Halos Produced at Low Dust Content

We report on new imaging observations of the Lyman alpha emission line (Lya), performed with the Hubble Space Telescope, that comprise the backbone of the Lyman alpha Reference Sample (LARS). We present images of 14 starburst galaxies at redshifts 0.028 < z < 0.18 in continuum-subtracted Lya, Halpha, and the far ultraviolet continuum. We show that Lya is emitted on scales that systematically exceed those of the massive stellar population and recombination nebulae: as measured by the Petrosian 20 percent radius, RP20, Lya radii are larger than those of Halpha by factors ranging from 1 to 3.6, with an average of 2.4. The average ratio of Lya-to-FUV radii is 2.9. This suggests that much of the Lya light is pushed to large radii by resonance scattering. Defining the "Relative Petrosian Extension" of Lya compared to Halpha, \xi_ext = RP20_Lya / RP20_Ha, we find \xi_ext to be uncorrelated with total Lya luminosity. However \xi_ext is strongly correlated with quantities that scale with dust content, in the sense that a low dust abundance is a necessary requirement (although not the only one) in order to spread Lya photons throughout the interstellar medium and drive a large extended Lya halo.Comment: Published in ApJ Letters ~~ 6 pages using emulateapj, 4 figures ~~ Higher-resolution, larger, nicer jpeg versions of Figures 1 and 2 can be found here: http://xayes.org/pub/press_lars.htm

Effect of nitrification inhibitors on the growth and activity of Nitrosotalea devanaterra in culture and soil

Peer reviewedPublisher PD

Spectroscopic Signatures of the Tidal Disruption of Stars by Massive Black Holes

During the tidal disruption of a star by a massive black hole (BH) of mass MBH <~ 10^7 Msun, stellar debris falls back to the BH at a rate well above the Eddington rate. A fraction of this gas is subsequently blown away from the BH, producing an optically bright flare of radiation. We predict the spectra and spectral evolution of tidal disruption events, focusing on the photoionized gas outside this outflow's photosphere. The spectrum will show absorption lines that are strongly blueshifted relative to the host galaxy, very broad (0.01-0.1c), and strongest at UV wavelengths (e.g., C IV, Ly alpha, O VI), lasting ~ 1 month for a 10^6 Msun BH. Meanwhile, supernovae in galactic nuclei are a significant source of confusion in optical surveys for tidal disruption events: we estimate that nuclear Type Ia supernovae are two orders of magnitude more common than tidal disruption events at z ~ 0.1 for ground-based surveys. Nuclear Type II supernovae occur at a comparable rate but can be excluded by pre-selecting red galaxies. Supernova contamination can be reduced to a manageable level using high-resolution follow-up imaging with adaptive optics or the Hubble Space Telescope. Our predictions should help optical transient surveys capitalize on their potential for discovering tidal disruption events.Comment: 14 pages, 5 figures; accepted for publication in MNRAS; some added discussion in Section

The Lyman Alpha Reference Sample: III. Properties of the Neutral ISM from GBT and VLA Observations

We present new H I imaging and spectroscopy of the 14 UV-selected star-forming galaxies in the Lyman Alpha Reference Sample (LARS), aimed for a detailed study of the processes governing the production, propagation, and escape of Ly$\alpha$ photons. New H I spectroscopy, obtained with the 100m Green Bank Telescope (GBT), robustly detects the H I spectral line in 11 of the 14 observed LARS galaxies (although the profiles of two of the galaxies are likely confused by other sources within the GBT beam); the three highest redshift galaxies are not detected at our current sensitivity limits. The GBT profiles are used to derive fundamental H I line properties of the LARS galaxies. We also present new pilot H I spectral line imaging of 5 of the LARS galaxies obtained with the Karl G. Jansky Very Large Array (VLA). This imaging localizes the H I gas and provides a measurement of the total H I mass in each galaxy. In one system, LARS 03 (UGC 8335 or Arp 238), VLA observations reveal an enormous tidal structure that extends over 160 kpc from the main interacting systems and that contains $>$10$^9$ M$_{\odot}$ of H I. We compare various H I properties with global Ly$\alpha$ quantities derived from HST measurements. The measurements of the Ly$\alpha$ escape fraction are coupled with the new direct measurements of H I mass and significantly disturbed H I velocities. Our robustly detected sample reveals that both total H I mass and linewidth are tentatively correlated with key Ly$\alpha$ tracers. Further, on global scales, these data support a complex coupling between Ly$\alpha$ propagation and the H I properties of the surrounding medium.Comment: Preprint form, 16 figures, accepted in Ap

The unusual NIV]-emitter galaxy GDS J033218.92-275302.7: star formation or AGN-driven winds from a massive galaxy at z=5.56

Aims: We investigate the nature of the source GDS J033218.92-275302.7at redshift ~ 5.56. Methods: The SED of the source is well sampled by 16 bands photometry, from UV-optical, near infrared and mid-infrared (MID-IR).The detection of signal in the MID-IR Spitzer/IRAC bands 5.8, 8.0 um -- where the nebular emission contribution is less effective -- suggests the presence of a Balmer break, signature of an underlying stellar population formed at earlier epochs. The optical spectrum shows a clear Lya emission line together with semi-forbidden NIV] 1483.3-1486.5 also in emission. Results: From the SED fitting and the Lya modelling it turns out that the source seems to have an evolved component with stellar mass of ~5 x10^(10) Msolar and age ~ 0.4 Gyrs, and a young component with an age of ~ 0.01 Gyrs and SFR in the range of 30-200 Msolar yr^(-1). The limits on the effective radius derived from the ACS/z850 and VLT/Ks bands indicate that this galaxy is denser than the local ones with similar mass. A relatively high nebular gas column density is favored from the Lya line modelling (NHI>=10^(21) cm^(-2)). A vigorous outflow (~ 450 km/s) has been measured from the optical spectrum,consistent with the Lya modelling. From ACS observations it turns out that the region emitting Lya photons is spatially compact and of the same order of the effective radius estimated at the ~1400A rest-frame wavelength, whose emission is dominated by the stellar continuum and/or AGN. The gas is blown out from the central region,but given the mass of the galaxy it is uncertain whether it will pollute the IGM to large distances. We argue that a burst of star formation in a dense gas environment is active (possibly containing hot and massive stars and/or a low luminosity AGN), superimposed to an already formed fraction of stellar mass (abridged).Comment: 13 pages, 9 figures (published on A&A). Here replaced with a typo fixed in the footnote of Sect. 4.2 and with four updated references. Results unchange