Reconstructing Asian faunal introductions to eastern Africa from multi-proxy biomolecular and archaeological datasets

Human-mediated biological exchange has had global social and ecological impacts. In subS-aharan Africa, several domestic and commensal animals were introduced from Asia in the pre-modern period; however, the timing and nature of these introductions remain contentious. One model supports introduction to the eastern African coast after the mid-first millennium CE, while another posits introduction dating back to 3000 BCE. These distinct scenarios have implications for understanding the emergence of long-distance maritime connectivity, and the ecological and economic impacts of introduced species. Resolution of this longstanding debate requires new efforts, given the lack of well-dated fauna from high-precision excavations, and ambiguous osteomorphological identifications. We analysed faunal remains from 22 eastern African sites spanning a wide geographic and chronological range, and applied biomolecular techniques to confirm identifications of two Asian taxa: domestic chicken (Gallus gallus) and black rat (Rattus rattus). Our approach included ancient DNA (aDNA) analysis aided by BLAST-based bioinformatics, Zooarchaeology by Mass Spectrometry (ZooMS) collagen fingerprinting, and direct AMS (accelerator mass spectrometry) radiocarbon dating. Our results support a late, mid-first millennium CE introduction of these species. We discuss the implications of our findings for models of biological exchange, and emphasize the applicability of our approach to tropical areas with poor bone preservation

Found in the Folds: A Rediscovery of Ancient Egyptian Pleated Textiles and the Analysis of Carbohydrate Coatings

Charles T. Currelly, first director of the Royal Ontario Museum, participated in excavations of the tomb of King Nebhepetre, now known as Mentuhotep II, (Dynasty XI) in Deir el-Bahri, Egypt in 1906. He brought to Canada many objects from the excavations, and objects that he purchased while in Egypt; these formed the initial collection of the museum. Among the objects were seven fragments of fine linen cloth with intricate pleat patterns. Recently, the cloths became the subject of a study to learn how they had retained their pleats for 4000 years. Samples were examined and analysed using polarised light microscopy, scanning electron microscopy-electron dispersive X-ray spectrometry, gas chromatography-mass spectrometry, and pyrolysis-gas chromatography-mass spectrometry. Three of the cloths were likely fragments of clothing re-purposed as bandages and were found to be saturated in mummification balms composed of Pinaceae resin, Pistacia resin, and an essential oil characterised by a high abundance of cedrol, possibly originating from a juniper species. All seven of the cloths were found to have traces of polysaccharides from two probable sources: an arabinogalactan gum such as gum arabic or a fruit gum, and a polyglucoside, possibly starch

From photogrammetry to radiocarbon dating; investigating hafting adhesives on stone tools using a multi-analytical approach

International audienc

Molecular profiling of Peru Balsam reveals active ingredients responsible for its pharmaceutical properties

International audienc

Recipes of Ancient Egyptian kohls more diverse than previously thought.

Kohl, a dark eye cosmetic, is a well-known part of Ancient Egyptian culture. Modern chemical analyses of kohls have largely found lead-based inorganic constituents, whereas earlier studies argued for a much broader range of constituents. Furthermore, organic materials in kohls remain severely understudied. This raises questions regarding the true diversity of materials and recipes used to produce kohls. We analysed the contents of 11 kohl containers from the Petrie Museum collection in London. The objects selected cover a broad range of times and locations in Egypt. Our multi-analytical approach allowed us to characterise both inorganic and organic components. Our data show that inorganic ingredients in kohl recipes are not only lead-based but also manganese- and silicon-based. Our analyses also revealed that organic ingredients derived from both plant and animal sources were commonly used in kohl recipes and sometimes even represent the main constituent. All these findings point towards more varied recipes than initially thought and significantly shift our understanding of Ancient Egyptian kohls

Use of Zanzibar copal (Hymenaea verrucosa Gaertn.) as incense at Unguja Ukuu, Tanzania in the 7-8th century CE: Chemical insights into trade and Indian Ocean interactions

This study presents the chemical analysis of an amorphous organic residue extracted from a 7th-early 8th century CE brass artefact from the trading port of Unguja Ukuu, Zanzibar, Tanzania, hypothesised to be an incense burner. The artefact is a very rare and highly significant find in East Africa, with only one other example being found previously (also at the same site), and likely represents early contact between coastal East Africa and the Indian Ocean world. Chemical analysis of the residue adhering to this artefact was undertaken to confirm its use to burn incense, and to determine whether the resin used was local or exotic to East Africa and thus likely acquired through long-distance trade. The residue extract was analysed by gas chromatography-mass spectroscopy (GC-MS) and identified as Zanzibar copal (Hymenaea verrucosa Gaertn.), a local species that rose to major importance in colonial period trade. The results obtained from this study provide the first direct archaeological evidence for the ancient use of this East African species as an aromatic, suggesting that it might have had a much earlier role in long-distance incense trade than previously demonstrated. This finding also provides insights into local East African engagement with the material culture of the Indian Ocean world

Presumptive blood test using Hemastix® with EDTA in archaeology

A presumptive test is a rapid screening method that indicates the possible identity of a substance. To ensure a presumptive test is reliable, accurate and replicable, it has to be validated. In this paper we present a method validation for the cost-effective, presumptive blood test, Hemastix® with sodium ethylenediaminetetraacetic acid (EDTA) specifically for archaeology and buried residues. Hemastix®, a haemoglobin specific chemical reagent test strip (Hb-CRTS), was used with the addition of a chelating agent (EDTA) which increases the specificity of this test. It was conducted using a collection of 146 substances (plant, animal, metal compounds, chemical solutions including blood), 6 experimentally degraded samples, 177 experimental artefacts, 238 reference samples (on microscope slides) and 39 mixtures of substances. We also compared the Hemastix® and Hemastix® with EDTA test to two other Hb-CRTSs (URS-10 and Chemstrip® 10MD) and two additional presumptive tests, the tetramethylbenzidine (TMB) test and the phenolphthalein test. The results demonstrate that Hemastix® is the most specific, sensitive and cost-effective presumptive test evaluated which can be substituted with any other Hb-CRTS. Although the longevity and survival of haemoglobin on archaeological artefacts requires further investigation, the method validation described here supports Hemastix® performed with EDTA as being a reliable presumptive test for the presence of residual blood on buried artefacts

Hominin Dispersal into the Nefud Desert and Middle Palaeolithic Settlement along the Jubbah Palaeolake, Northern Arabia

International audienceThe Arabian Peninsula is a key region for understanding hominin dispersals and the effect of climate change on prehistoric demography, although little information on these topics is presently available owing to the poor preservation of archaeological sites in this desert environment. Here, we describe the discovery of three stratified and buried archaeological sites in the Nefud Desert, which includes the oldest dated occupation for the region. The stone tool assemblages are identified as a Middle Palaeolithic industry that includes Levallois manufacturing methods and the production of tools on flakes. Hominin occupations correspond with humid periods, particularly Marine Isotope Stages 7 and 5 of the Late Pleistocene. The Middle Palaeolithic occupations were situated along the Jubbah palaeolake-shores, in a grassland setting with some trees. Populations procured different raw materials across the lake region to manufacture stone tools, using the implements to process plants and animals. To reach the Jubbah palaeolake, Middle Palaeolithic populations travelled into the ameliorated Nefud Desert interior, possibly gaining access from multiple directions, either using routes from the north and west (the Levant and the Sinai), the north (the Mesopotamian plains and the Euphrates basin), or the east (the Persian Gulf). The Jubbah stone tool assemblages have their own suite of technological characters, but have types reminiscent of both African Middle Stone Age and Levantine Middle Palaeolithic industries. Comparative interregional analysis of core technology indicates morphological similarities with the Levantine Tabun C assemblage, associated with human fossils controversially identified as either Neanderthals or Homo sapiens

Hominin Dispersal into the Nefud Desert and Middle Palaeolithic Settlement along the Jubbah Palaeolake, Northern Arabia

The Arabian Peninsula is a key region for understanding hominin dispersals and the effect of climate change on prehistoric demography, although little information on these topics is presently available owing to the poor preservation of archaeological sites in this desert environment. Here, we describe the discovery of three stratified and buried archaeological sites in the Nefud Desert, which includes the oldest dated occupation for the region. The stone tool assemblages are identified as a Middle Palaeolithic industry that includes Levallois manufacturing methods and the production of tools on flakes. Hominin occupations correspond with humid periods, particularly Marine Isotope Stages 7 and 5 of the Late Pleistocene. The Middle Palaeolithic occupations were situated along the Jubbah palaeolake-shores, in a grassland setting with some trees. Populations procured different raw materials across the lake region to manufacture stone tools, using the implements to process plants and animals. To reach the Jubbah palaeolake, Middle Palaeolithic populations travelled into the ameliorated Nefud Desert interior, possibly gaining access from multiple directions, either using routes from the north and west (the Levant and the Sinai), the north (the Mesopotamian plains and the Euphrates basin), or the east (the Persian Gulf). The Jubbah stone tool assemblages have their own suite of technological characters, but have types reminiscent of both African Middle Stone Age and Levantine Middle Palaeolithic industries. Comparative inter-regional analysis of core technology indicates morphological similarities with the Levantine Tabun C assemblage, associated with human fossils controversially identified as either Neanderthals or Homo sapiens

View of Jebel Umm Sanman and location of excavation units at JSM-1.

<p>The Jebel Umm Sanman is the largest jebel in the area. The modern town of Jubbah is developing on the former lake bed. Note the location of yellow quartzite seam relative to artefact finds.</p