Transcriptome analysis of copper stress response in rice seedling using DNA microarray

Heavy metal contamination along with the increase in food demand are a primary concern in Vietnam and all over the world. In order to enhance crop tolerance to unfavorable cultivation conditions including heavy metal toxicity, understanding of plant response system under the effect of heavy metals is necessary. In the current study, physiological, biochemical and transcriptomic changes of rice seedings (Oryza sativa L. cv. IR64) were investigated under copper (Cu) stress. Root elongation and root fresh weight were decreased whereas accumulation of copper in root was enhanced significantly with increasing copper concentration from 2.5 to 15 M. In addition, copper induced endogenous reactive oxygen species (ROS) generation and activated isoenzymes of superoxide dismutase (SOD) and catalase (CAT). The molecular mechanism of rice roots in response to copper toxicity at mRNA expression level was analyzed by microarray technique. Functions and roles of genes were also analyzed by bioinformatic tools AgriGO and MapMan. Gene ontology analysis revealed that 1900 Cu responsive genes were involved in phytohormones, reactive oxygen species, signaling pathways, transcription factors, transport activities, antioxidant defense systems. Through phytohormones and reactive oxygen species, Cu may inhibit rice root growth. Phytohormones and reactive oxygen species can also be signal molecules in signaling pathways with the participation of mitogen-activated protein kinase (MAPK) cascades, and transcription factors in response to Cu stress. Detoxification and protection mechanisms may involve transport activities and antioxidant defense systems during Cu treatment. These results may provide new insights into mechanisms of rice plant to tolerate with Cu toxicity conditions

The role of classroom observation in the development and assessment of schoolteachers in Vietnam: a review of national policy and research

This paper focuses on the role of classroom observation in the development and assessment of schoolteachers in Vietnam through a narrative review of current policy and cognate research literature. The overall aim of this review was twofold. Firstly, to contribute to a growing bank of Vietnam-based studies to maximise the value of the insights from this scholarly work into recent policy reforms on teachers’ professional development in Vietnam. Secondly, to widen access and exposure to published work on the topic in Vietnamese that is not readily accessible to English-speaking scholars. This review revealed a long history and engrained culture of using observation as a teacher performance evaluation tool. In recent years, there have been policy reforms and research studies that have repositioned observation as a tool for teacher learning and development. However, the implementation of these reforms has been inconsistent across Vietnam, along with recent reform having encountered resistance from a culture of compliance in schools. This paper identifies some of the key issues that policy makers and educational leaders need to address in practice in order to ensure the effective and meaningful implementation of the reforms relating to the use of observation for learning and developmental purposes

Sorting or supporting teachers? An exploration of the imbalanced role of classroom observation in the development and assessment of Vietnamese secondary schoolteachers

In 2018, Vietnam’s National Teacher Education Programme established a new framework for schoolteachers’ professional development. Classroom observation was the dominant method used for teacher monitoring, assessment and development. This paper explores the use and impact of current classroom observation policy and practice on novice teachers in Vietnamese secondary schools. Our study draws on qualitative data from 35 semi-structured interviews across four provinces. Our findings revealed ongoing policy-practice tensions, with the prioritisation and dominance of classroom observation as an assessment tool for sorting rather than supporting teachers. These tensions were indicative of a wider policy-practice disconnect enshrined in recent reform by the Ministry of Education and Training that advocated the adoption of lesson study as the preferred national approach in schools. However, the experiences of our participants reflected a very different picture in practice that reinforced traditional hierarchies of power associated with evaluative models of observation that are designed to standardise and rank teachers’ classroom performance rather than develop the quality of teaching. Instead of the more collaborative and collegial ethos typically associated with inquiry-based approaches like lesson study, observation was being used largely as a high-stakes assessment, resulting in increased levels of anxiety and stress among teachers

ARSENIC POLLUTION IN GROUNDWATER IN RED RIVER DELTA, VIETNAM : SITUATION AND HUMAN EXPOSURE

Joint Research on Environmental Science and Technology for the Eart

Host Transcription Profile in Nasal Epithelium and Whole Blood of Hospitalized Children Under 2 Years of Age With Respiratory Syncytial Virus Infection.

BACKGROUND: Most insights into the cascade of immune events after acute respiratory syncytial virus (RSV) infection have been obtained from animal experiments or in vitro models. METHODS: In this study, we investigated host gene expression profiles in nasopharyngeal (NP) swabs and whole blood samples during natural RSV and rhinovirus (hRV) infection (acute versus early recovery phase) in 83 hospitalized patients <2 years old with lower respiratory tract infections. RESULTS: Respiratory syncytial virus infection induced strong and persistent innate immune responses including interferon signaling and pathways related to chemokine/cytokine signaling in both compartments. Interferon-α/β, NOTCH1 signaling pathways and potential biomarkers HIST1H4E, IL7R, ISG15 in NP samples, or BCL6, HIST2H2AC, CCNA1 in blood are leading pathways and hub genes that were associated with both RSV load and severity. The observed RSV-induced gene expression patterns did not differ significantly in NP swab and blood specimens. In contrast, hRV infection did not as strongly induce expression of innate immunity pathways, and significant differences were observed between NP swab and blood specimens. CONCLUSIONS: We conclude that RSV induced strong and persistent innate immune responses and that RSV severity may be related to development of T follicular helper cells and antiviral inflammatory sequelae derived from high activation of BCL6

A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity.

Keratin intermediate filaments (KIFs) protect the epidermis against mechanical force, support strong adhesion, help barrier formation, and regulate growth. The mechanisms by which type I and II keratins contribute to these functions remain incompletely understood. Here, we report that mice lacking all type I or type II keratins display severe barrier defects and fragile skin, leading to perinatal mortality with full penetrance. Comparative proteomics of cornified envelopes (CEs) from prenatal KtyI(-/-) and KtyII(-/-)(K8) mice demonstrates that absence of KIF causes dysregulation of many CE constituents, including downregulation of desmoglein 1. Despite persistence of loricrin expression and upregulation of many Nrf2 targets, including CE components Sprr2d and Sprr2h, extensive barrier defects persist, identifying keratins as essential CE scaffolds. Furthermore, we show that KIFs control mitochondrial lipid composition and activity in a cell-intrinsic manner. Therefore, our study explains the complexity of keratinopathies accompanied by barrier disorders by linking keratin scaffolds to mitochondria, adhesion, and CE formation

Analysis of Antimicrobial Resistance in Non-typhoidal Salmonella Collected From Pork Retail Outlets and Slaughterhouses in Vietnam Using Whole Genome Sequencing.

Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a "last defense" antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR

Th17 cells in human disease

Our understanding of the role of T cells in human disease is undergoing revision as a result of the discovery of T-helper 17 (Th17) cells, a unique CD4 + T-cell subset characterized by production of interleukin-17 (IL-17). IL-17 is a highly inflammatory cytokine with robust effects on stromal cells in many tissues. Recent data in humans and mice suggest that Th17 cells play an important role in the pathogenesis of a diverse group of immune-mediated diseases, including psoriasis, rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, and asthma. Initial reports also propose a role for Th17 cells in tumorigenesis and transplant rejection. Important differences, as well as many similarities, are emerging when the biology of Th17 cells in the mouse is compared with corresponding phenomena in humans. As our understanding of human Th17 biology grows, the mechanisms underlying many diseases are becoming more apparent, resulting in a new appreciation for both previously known and more recently discovered cytokines, chemokines, and feedback mechanisms. Given the strong association between excessive Th17 activity and human disease, new therapeutic approaches targeting Th17 cells are highly promising, but the potential safety of such treatments may be limited by the role of these cells in normal host defenses against infection.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72455/1/j.1600-065X.2008.00628.x.pd