Utility of EST-SNP Markers for Improving Management and Use of Olive Genetic Resources: A Case Study at the Worldwide Olive Germplasm Bank of Córdoba

Olive, the emblematic Mediterranean fruit crop, owns a great varietal diversity, which is maintained in ex situ field collections, such as the World Olive Germplasm Bank of Córdoba (WOGBC), Spain. Accurate identification of WOGBC, one of the world’s largest collections, is essential for efficient management and use of olive germplasm. The present study is the first report of the use of a core set of 96 EST-SNP markers for the fingerprinting of 1273 accessions from 29 countries, including both field and new acquired accessions. The EST-SNP fingerprinting made possible the accurate identification of 668 different genotypes, including 148 detected among the new acquired accessions. Despite the overall high genetic diversity found at WOGBC, the EST-SNPs also revealed the presence of remarkable redundant germplasm mostly represented by synonymy cases within and between countries. This finding, together with the presence of homonymy cases, may reflect a continuous interchange of olive cultivars, as well as a common and general approach for their naming. The structure analysis revealed a certain geographic clustering of the analysed germplasm. The EST-SNP panel under study provides a powerful and accurate genotyping tool, allowing for the foundation of a common strategy for efficient safeguarding and management of olive genetic resources.info:eu-repo/semantics/publishedVersio

Utility of EST-SNP Markers for Improving Management and Use of Olive Genetic Resources: A Case Study at the Worldwide Olive Germplasm Bank of Córdoba

Olive, the emblematic Mediterranean fruit crop, owns a great varietal diversity, which is maintained in ex situ field collections, such as the World Olive Germplasm Bank of Córdoba (WOGBC), Spain. Accurate identification of WOGBC, one of the world’s largest collections, is essential for efficient management and use of olive germplasm. The present study is the first report of the use of a core set of 96 EST-SNP markers for the fingerprinting of 1273 accessions from 29 countries, including both field and new acquired accessions. The EST-SNP fingerprinting made possible the accurate identification of 668 different genotypes, including 148 detected among the new acquired accessions. Despite the overall high genetic diversity found at WOGBC, the EST-SNPs also revealed the presence of remarkable redundant germplasm mostly represented by synonymy cases within and between countries. This finding, together with the presence of homonymy cases, may reflect a continuous interchange of olive cultivars, as well as a common and general approach for their naming. The structure analysis revealed a certain geographic clustering of the analysed germplasm. The EST-SNP panel under study provides a powerful and accurate genotyping tool, allowing for the foundation of a common strategy for efficient safeguarding and management of olive genetic resources.EEA San JuanFil: Belaj, Angjelina. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA) . Centro “Alameda del Obispo”; EspañaFil: Ninot, Antònia . IRTA; EspañaFil: Gómez-Gálvez, Francisco J. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA) . Centro “Alameda del Obispo”; EspañaFil: El Riachy, Milad. LARI. Department of Olive and Olive Oil; LíbanoFil: Gurbuz-Veral, Melek. Olive Research Institute. Department of Breeding and Genetics; TurquíaFil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lazaj, Adhurim. Qendra e Transferimit te Teknologjise Bujqesore; AlbaniaFil: Klepo, Tatjana.Croatian Agency for Agriculture and Food. Center of Pomology; CroaciaFil: Paz, Sergio. Servicio de Transferencia de Tecnología Conselleria de Agricultura, Desarrollo Rural, Emergencia Climática y Transición Ecológica; EspañaFil: Ugarte, Javier. Gobierno de La Rioja. Servicio de Investigación Agraria y Sanidad Vegetal; EspañaFil: Baldoni, Luciana. National Research Council. Institute of Biosciences and Bioresources; ItaliaFil: Lorite, Ignacio J. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA) . Centro “Alameda del Obispo”; EspañaFil: Šatović, Zlatko .University of Zagreb. Department of Seed Science and Technology, Faculty of Agriculture; CroaciaFil: Šatović, Zlatko. Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv); CroaciaFil: de la Rosa, Raúl . Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA) . Centro “Alameda del Obispo”; Españ

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Design and construction of a large weighing lysimeter in an almond orchard

Effective water management is essential to ensure the sustainability of irrigated agriculture. The accurate determination of crop water requirements is the first step in this task. This paper describes the building of a one-tree weighing lysimeter (3m x 3m and 2.15m depth) located in an almond orchard (Prunus dulcis �Guara�), inside the experimental farm �Alameda del Obispo� in Córdoba, Spain, to measure orchard evapotranspiration (ETc). Following a review on lysimetry, the description of the construction of the weighing lysimeter is provided in detail, including considerations relative to system resolution and wind effects on the measurements. Finally, some preliminary results of the evaporation and transpiration of young almond trees are presented demonstrating that lysimetry in orchards provides accurate ET values needed to determine irrigation water requirements

Using remote sensing techniques at different resolution scales for enhancing irrigation management

Currently there is a growing interest in improving water management in Mediterranean agriculture due to the foreseeable results of climate change and to the competition with other sectors such as the environmental. For this reason different methodologies have been evaluated in this thesis to increase water use efficiency in Andalusian agriculture by means of the improvement in the estimation of crop irrigation water requirements, using different remote sensing techniques and spatial analysis. In this work the two main parameters involved in crop evapotranspiration determination were addressed: reference evapotranspiration (Chapters 1 and 2) and crop coefficient (Chapters 3 and 4).Actualmente existe un inter&eacute;s creciente por la mejora de la gesti&oacute;n del agua en la agricultura mediterr&aacute;nea debido a las previsibles consecuencias del cambio clim&aacute;tico y a la competencia con otros sectores como el medioambiental. Por este motivo en esta tesis se han evaluado diferentes metodolog&iacute;as para incrementar la eficiencia en el uso del agua en la agricultura andaluza por medio de la mejora en la estimaci&oacute;n de las necesidades de riego de los cultivos, empleando diferentes t&eacute;cnicas de teledetecci&oacute;n y an&aacute;lisis espacial. De este modo, en este trabajo se abord&oacute; el estudio de los dos principales par&aacute;metros involucrados en la determinaci&oacute;n de la evapotranspiraci&oacute;n de cultivo: la evapotranspiraci&oacute;n de referencia (Cap&iacute;tulos 1 y 2) y el coeficiente de cultivo (Cap&iacute;tulos 3 y 4).Programa de Doctorado en Ingeniería Agraria, Alimentaria, Forestal y del Desarrollo Rural Sostenible por la Universidad de Córdoba y la Universidad de Sevilla. Dirección: Ignacio J. Lorite (IFAPA) y Cristina Santos (IFAPA). Mención Europeo / Mención Internacional: Concedid

Phenological diversity in a World Olive Germplasm Bank: Potential use for breeding programs and climate change studies

Aim of study: Crop phenology is a critical component in the identification of impacts of climate change. Then, the assessment of germplasm collections provides relevant information for cultivar selection and breeding related to phenology, being the base for identifying adaptation strategies to climate change.Area of study: The World Olive Germplasm Bank located at IFAPA Centre “Alameda del Obispo” (WOGB-IFAPA) in Cordoba (Southern Spain) was considered for the study.Material and methods: Data gathered for nine years on flowering and ripening time of olive cultivars from WOGB-IFAPA were evaluated. Thus, full flowering date (FFD) for 148 cultivars and ripening date (RD) for 86 cultivars, coming from 14 olive growing countries, were considered for characterization of olive phenology and for calibration/validation of phenological models.Main results: The characterization of WOGB-IFAPA has allowed the identification of cultivars with extreme early (‘Borriolenca’) and late (‘Ulliri i Kuq’) flowering as well as the ones with extreme early (‘Mavreya’) and late (‘Gerboui’) ripening dates. However, the very limited inter-cultivar variability, especially for FFD, resulted in a non-optimal simulation models performance. Thus, for FFD and RD the root mean square error was around 6 and 24 days, respectively. The limited inter-cultivar variability was associated to the low average temperatures registered during winter at WOGB-IFAPA generating an early accumulation of the chilling requirements, thus homogenizing FFD of all the analyzed cultivars.Research highlights: The identification of cultivars with early FFD and late RD provides useful information for breeding programs and climate change studies for identifying adaptation strategies

Wheat yield sensitivity to climate change across a European transect for a large ensemble of crop models

peer reviewedFACCE-JPI MACSUR Modelling European Agriculture with Climate Change for Food Securit