Reproductive Biology and Ecology of the Endemic New Zealand Tree Ixerba brexioides (tāwari)

This research investigated the ecology of Ixerba brexioides (tāwari) with regard to pollination, breeding strategy, seed biology, and forest composition. Research was focused around three main questions: 1. How is tāwari pollinated with regard to vector and pollen source? How is it adapted for this? 2. How is seed dispersal of tāwari achieved and under what conditions is germination most successful? 3. What are the dominant community associations of tāwari what how are they constrained by environmental variables? The first research question was addressed using video surveillance, nectar analysis, and artificial pollination experiments in a small tract of tāwari forest at Tūī Ridge Park in the Mamaku Range, North Island, New Zealand. Analysis of 125 hours of video footage showed that tāwari is predominantly insect pollinated with occasional bird visitation. The most frequent flower visitors were flies and nocturnal moths. Tāwari nectar volume peaked at midday, and declined toward the late afternoon, before increasing again at dusk. This pattern of nectar secretion followed closely the activity patterns of flies during the day and moths at night. Nectar sugar concentration was 11% on average (range 3% to 20%) which is considered low, but is suited to moths, bats, birds, and bees. Exclusion experiments demonstrated that tāwari is capable of producing viable seed under cross-fertilisation, self-fertilisation, and agamospermy. Breeding system indices demonstrated that tāwari is medium pollen limited (PLI = 0.31), self-compatible (SCI = 0.93), and autonomously selfing (ASI = 0.65). Question two was addressed by video surveillance of tāwari seed capsules, morphological observation, and by a series of germination experiments. Video failure meant that with limited footage (mostly at night) no dispersal activity was captured on film. However, using available literature and observations of seed morphology birds were considered the most probable effective disperser. Germination trials included standard conditions, shade, seeds left in fruit, seeds deposited on soil surface, and seeds buried at 5 cm soil depth. Germination was most effective in the buried treatment (average 85% germination) and the soil surface treatment (average 75% germination). Cluster analysis and NMS (Non-Metric Multi-Dimensional Scaling) ordination of 641 plots of tāwari forest from the NVS (National Vegetation Survey) database was used to assess the community associations of tāwari. Based on plot species assemblage, the analysis showed four main forest types that are largely separate in geographical space: Northland, Coromandel, Kaimai, and Urewera. Each type had significant indicator species that were constrained in range by latitudinal limits. Recent literature suggests that environmental variables that most influence tāwari forest distribution are annual temperature and rainfall in conjunction with solar radiation. Tāwari forest occupies only areas which are cool and moist, and seedlings are biased toward high light conditions. In the central North Island tāwari had the highest probability of occurrence in sites with a mean annual temperature of 11° to 13° Celsius, mean annual rainfall between 2000 and 2250 mm annually, and mean solar radiation of 143 to 145 MJ m2 per day. Parent material also plays an important part, especially the depth of Taupō Pumice deposits as tāwari forest is more prone to occur in areas of mature soil where depth of volcanic deposits is not excessive. Information on tāwari from the present thesis combined with other published and unpublished sources is presented in the form of a New Zealand Biological Flora Series journal contribution. Recommendations for further research on tāwari and New Zealand reproductive biology in general are given

Key ecological sites of Hamilton City: Volume 1

Ecological sites of significance previously identified in 2000 were reviewed in 2011. Natural vegetation in areas acquired by the city since 2000 was also surveyed to identify any new key sites. In total seventy key sites that met the Waikato Regional Council Regional Policy Statement criteria for ecological significance were identified across Hamilton City. Of the original key sites, the total area covered by sites, average site size and overall quality of sites had increased between the 2000 and 2011 surveys. This was due to restoration efforts across the city by Hamilton City Council and the community. Vegetation restoration efforts have had other biodiversity and ecological benefits such as providing additional habitat for the city’s increasing tui population. Key sites are not spread evenly across the city or across landform types. Most key sites are either in gullies or adjoining the Waikato River. Less than 1% of urban alluvial plains and peat bogs are key sites. Two sites on private land have degraded and no longer meet the ecological significance criteria in 2011. The current survey utilised a standard methodology focused on vegetation types. There will be other significant sites not identified including sites with significant fauna values but a detailed and costly survey would be required to identify all such sites. The 1.5% of the city area covered by key sites is well below the 10% minimum recommended to prevent biodiversity decline in urban areas. Areas where vegetation restoration has begun in the city have the potential to expand existing key sites or develop new sites if council and community efforts continue in the future. The Council and its restoration partners should continue to seek ways of increasing native vegetation cover in Hamilton City and restoration of the distinctive gully landform remains the best option

Project Re•center dot Vision: disability at the edges of representation

The representational history of disabled people can largely be characterized as one of being put on display or hidden away. Self-representations have been a powerful part of the disability rights and culture movement, but recently scholars have analysed the ways in which these run the risk of creating a ‘single story’ that centres the experiences of white, western, physically disabled men. Here we introduce and theorize with Project Re•Vision, our arts-based research project that resists this singularity by creating and centring, without normalizing, representations that have previously been relegated to the margins. We draw from body becoming and new materialist theory to explore the dynamic ways in which positionality illuminates bodies of difference and open into a discussion about what is at stake when these stories are let loose into the world

“Artlift” Arts-on-Referral Intervention in UK Primary Care: Updated findings from an ongoing observational study

Background: Arts for health interventions are an accepted option for medical management of mental wellbeing in health care. Updated findings are presented from a prospective longitudinal follow-up (observational) design study of an arts on referral programme in UK general practice, over a 7-year period (2009–2016). Methods: Primary care process and mental wellbeing outcomes were investigated, including progress through the intervention, changes in mental wellbeing, and factors associated with those outcomes. A total of n =1297 patients were referred to an eight or 10-week intervention over a period from 2009 to 2016. Patient sociodemographic information was recorded at baseline, and patient progress (e.g. attendance) assessed throughout the intervention. Results: Of all referrals, 51.7% completed their course of prescribed art (the intervention). Of those that attended, 74.7% engaged with the intervention as rated by the artists leading the courses. A significant increase in wellbeing was observed from pre- to post-intervention (t = −19.29, df =523, P < 0.001, two-tailed) for those that completed and/or engaged. A sub sample (N =103) of these referrals self-reported multi-morbidities. These multiple health care service users were majority completers (79.6%), and were rated as having engaged (81.0%). This group also had a significant increase in well-being, although this was smaller than for the group as a whole (t = −7.38, df =68, P < 0.001). Conclusion: Findings confirm that art interventions can be effective in the promotion of well-being for those that complete, including those referred with multi-morbidity, with significant changes in wellbeing evident across the intervention periods

Phylogenomics Reveals Ancient Gene Tree Discordance in the Amphibian Tree of Life

Molecular phylogenies have yielded strong support for many parts of the amphibian Tree of Life, but poor support for the resolution of deeper nodes, including relationships among families and orders. To clarify these relationships, we provide a phylogenomic perspective on amphibian relationships by developing a taxon-specific Anchored Hybrid Enrichment protocol targeting hundreds of conserved exons which are effective across the class. After obtaining data from 220 loci for 286 species (representing 94% of the families and 44% of the genera), we estimate a phylogeny for extant amphibians and identify gene tree–species tree conflict across the deepest branches of the amphibian phylogeny. We perform locus-by-locus genealogical interrogation of alternative topological hypotheses for amphibian monophyly, focusing on interordinal relationships. We find that phylogenetic signal deep in the amphibian phylogeny varies greatly across loci in a manner that is consistent with incomplete lineage sorting in the ancestral lineage of extant amphibians. Our results overwhelmingly support amphibian monophyly and a sister relationship between frogs and salamanders, consistent with the Batrachia hypothesis. Species tree analyses converge on a small set of topological hypotheses for the relationships among extant amphibian families. These results clarify several contentious portions of the amphibian Tree of Life, which in conjunction with a set of vetted fossil calibrations, support a surprisingly younger timescale for crown and ordinal amphibian diversification than previously reported. More broadly, our study provides insight into the sources, magnitudes, and heterogeneity of support across loci in phylogenomic data sets

Derivation of Induced Pluripotent Stem Cells from Human Peripheral Blood T Lymphocytes

Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs (“TiPS”) retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe

The preponderance of matter over antimatter in the early Universe, the dynamics of the supernova bursts that produced the heavy elements necessary for life and whether protons eventually decay --- these mysteries at the forefront of particle physics and astrophysics are key to understanding the early evolution of our Universe, its current state and its eventual fate. The Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed plan for a world-class experiment dedicated to addressing these questions. LBNE is conceived around three central components: (1) a new, high-intensity neutrino source generated from a megawatt-class proton accelerator at Fermi National Accelerator Laboratory, (2) a near neutrino detector just downstream of the source, and (3) a massive liquid argon time-projection chamber deployed as a far detector deep underground at the Sanford Underground Research Facility. This facility, located at the site of the former Homestake Mine in Lead, South Dakota, is approximately 1,300 km from the neutrino source at Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino charge-parity symmetry violation and mass ordering effects. This ambitious yet cost-effective design incorporates scalability and flexibility and can accommodate a variety of upgrades and contributions. With its exceptional combination of experimental configuration, technical capabilities, and potential for transformative discoveries, LBNE promises to be a vital facility for the field of particle physics worldwide, providing physicists from around the globe with opportunities to collaborate in a twenty to thirty year program of exciting science. In this document we provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess.Comment: Major update of previous version. This is the reference document for LBNE science program and current status. Chapters 1, 3, and 9 provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess. 288 pages, 116 figure

The state of the Martian climate

60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes

HNF4A and GATA6 loss reveals therapeutically actionable subtypes in pancreatic cancer

Pancreatic ductal adenocarcinoma (PDAC) can be divided into transcriptomic subtypes with two broad lineages referred to as classical (pancreatic) and squamous. We find that these two subtypes are driven by distinct metabolic phenotypes. Loss of genes that drive endodermal lineage specification, HNF4A and GATA6, switch metabolic profiles from classical (pancreatic) to predominantly squamous, with glycogen synthase kinase 3 beta (GSK3β) a key regulator of glycolysis. Pharmacological inhibition of GSK3β results in selective sensitivity in the squamous subtype; however, a subset of these squamous patient-derived cell lines (PDCLs) acquires rapid drug tolerance. Using chromatin accessibility maps, we demonstrate that the squamous subtype can be further classified using chromatin accessibility to predict responsiveness and tolerance to GSK3β inhibitors. Our findings demonstrate that distinct patterns of chromatin accessibility can be used to identify patient subgroups that are indistinguishable by gene expression profiles, highlighting the utility of chromatin-based biomarkers for patient selection in the treatment of PDAC