Diversidade e caracterização de Lepidópteros (Insec: Lepidoptera) associados a passifloras e identificação de fontes de resistência

Tese (doutorado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Programa de Pós-Graduação em Agronomia, 2019.A interação do gênero Passiflora com lepidópteros tem sido objeto de investigações, pois hospedam uma grande diversidade de artrópodes que podem provocar danos econômicos, reduzindo a produção de frutos ou causando morte das plantas. Objetivou-se, neste trabalho, avaliar a diversidade, realizar caracterização de lepidópteros em passifloráceas e identificar fontes de resistência de passifloras a lepidópteros. O estudo foi realizado na Unidade de Apoio da Fruticultura, no Laboratório de Entomologia, no Laboratório de Biologia Vegetal e no Banco Ativo de Germoplasma Flor da Paixão da Embrapa Cerrados. No primeiro capítulo foi realizado um estudo bibliográfico de lepidópteros associados em passifloráceas no Brasil e a variação temporal e das principais pragas, onde o levantamento foi útil para compreender a dinâmica das borboletas e subsidiar informações para estudos de manejo de pragas. No segundo capítulo foi analizado, reações de cultivares de maracujazeiro ornamental ao ataque de lepidópteros. Cultivares BRS Estrela do Cerrado, BRS Rubi do Cerrado e BRS Pérola do Cerrado demostraram resistência ao ataque de instos-praga, já a cultivar BRS Rósea Purpura apresentou maior porcentagem de área foliar consumida, sendo que um maior consumo foi verificado nas folhas localizadas nas regiões apicais e mediana dos ramos. No terceiro capítulo, foi verificado o consumo da área foliar de passifloráceas pela lagarta Agraulis vanillae vanillae, onde os acessos das espécies Passiflora alata, Passiflora glandulosa, Passiflora ambigua, Passiflora gardneri e Passilfora riparia, evidenciaram sua potencialidade como fontes de resistência a pragas. No quarto ao sexto capítulos, foram analizados a sobrevivência, resistência e preferência de S. frugiperda em cultivares de Passiflora L, no qual as espécies-cultivares P. incarnata, BRS Pérola do Cerrado, BRS Mel do Cerrado, BRS Maracujá Maçã e BRS Rósea Púrpura apresentaram maior nível de resistência a esta praga. No sétimo capitulo foi reportado o primeiro registro de ocorrência de algumas espécies como, Isia alcumena, Spodoptera cosmioides e Spodoptera eridania atacando plantas de maracujazeiro azedo (Passiflora edulis) no Brazil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).The interaction of the genus Passiflora with lepidoptera has been the subject of research, as they host a large diversity of arthropods that can cause economic damage, reducing fruit production or causing plant death. The objective of this work was to evaluate the diversity, to characterize lepidoptera in passiflora and to identify sources of resistance of passiflora to lepidoptera. The study was conducted at the Fruit Support Unit, the Entomology Laboratory, the Plant Biology Laboratory and the Embrapa Cerrado Active Passion Flower Germplasm Bank. In the first chapter, a bibliographic study of associated lepidoptera in passiflora in Brazil and the temporal variation and the main pests was carried out, where the survey was useful to understand the dynamics of butterflies and to support information for pest management studies. In the second chapter it was analyzed reactions of ornamental passion fruit cultivars to the attack of lepidoptera. Cultivars BRS Estrela do Cerrado, BRS Rubi do Cerrado and BRS Pearl of the Cerrado showed resistance to attack by pests, whereas the cultivar BRS Rósea Purpura had a higher percentage of leaf area consumed, and higher consumption was observed in the leaves located in the regions. apical and median of the branches. In the third chapter, the consumption of the passiflora leaf area by the caterpillar Agraulis vanillae vanillae was verified, where the accessions of the species Passiflora alata, Passiflora glandulosa, Passiflora ambigua, Passiflora gardneri and Passilfora riparia, evidenced their potentiality as sources of resistance to pests. In the fourth to sixth chapters, the survival, resistance and preference of S. frugiperda in Passiflora L cultivars were analyzed. Purple showed higher level of resistance to this pest. In the seventh chapter was reported the first record of occurrence of some species such as Isia alcumena, Spodoptera cosmioides and Spodoptera eridania attacking sour passion fruit (Passiflora edulis) plants in Brazil

Primeiro registro de Isia alcumena, Spodoptera cosmioides e Spodoptera eridania (Lepidoptera: Noctuoidea) em maracujazeiro (Passiflora edulis Sims) no Brasil

O Brasil é considerado o centro de origem e de diversidade das passifloras, que apresentam importância, tanto para o consumo in natura, industrialização, uso medicinal e também como plantas ornamentais. As diferentes espécies de Passiflora hospedam uma grande diversidade de artrópodes que, ocasionando injúrias, podem causar danos econômicos e morte da planta. Dentre os artrópodes, as lagartas-desfolhadoras são consideradas as pragas mais frequentes e severas nas principais regiões produtoras de maracujá. Assim, o objetivo foi informar o primeiro registro da ocorrência das espécies Isia alcumena, Spodoptera cosmioides e S. eridania atacando maracujazeiros no Distrito Federal, Brasil. As lagartas de S. cosmioides e S. eridania foram coletadas enquanto consumiam folhas de híbridos intraespecíficos de Passiflora edulis cultivado no banco ativo de germoplasma “Flor da Paixão” da Embrapa Cerrados. Já as lagartas de I. alcumena foram coletadas em folhas da cultivar BRS Rubi do Cerrado, na Unidade de Apoio da Fruticultura da Embrapa Cerrados. Após a coleta, as lagartas foram individualizadas em sistema de criação com folhas das plantas hospedeiras. O desenvolvimento das lagartas foi acompanhado até à emergência dos adultos, que foram identificados e fixados em alfinetes entomológicos para preservação permanente, a seco.Brazil is considered the center of origin and diversity of passifloras. These species have a great importance to in natura fruits consumption, industrialization, medicinal use and also as ornamental plants. The different Passiflora speciesare host of a great diversity of arthropods that can cause injuries, economic damages and, in some situations, the plant death. Among the arthropods, leafhopper caterpillars are considered frequent and severe pests in the main passion fruit producing regions. The present work is an occurrence report of Isia alcumena and Spodoptera cosmioides attacking passion fruit plants (Passiflora edulis Sims) in the Federal District, Brazil. The S. cosmioides and S. eridania caterpillars were collected while consuming leaves of Passiflora edulis intra-specific hybrid located in the Germplasm Active Bank ‘Flor da Paixão’ at Embrapa Cerrados. The caterpillars of I. alcumena were collected on leaves of the Passiflora edulis cv. BRS Rubi do Cerrado at the Agricultural Support Unit, Embrapa Cerrados. After collected, the caterpillars were individualized in a breeding system with leaves of the host plants. The development of the caterpillar was accompanied until the emergence of the adults, which were identified and fixed in entomological pins for permanent dry preservation

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase&nbsp;1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation&nbsp;disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age&nbsp; 6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score&nbsp; 652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc&nbsp;= 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N&nbsp;= 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in&nbsp;Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in&nbsp;Asia&nbsp;and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Key factors influencing the sale of bulls in livestock auctions

This research determines which factors most influence the purchase price of bulls in livestock auctions in the State of Rio Grande do Sul, Southern Brazil. Hence, 760 beef bulls sold in eleven different auctions between August and November 2013 were analysed. The data consists of: breed, muscularity (MUSC), frame (FRAME), body condition score (BCS), scrotal circumference (SC) and body weight (BW). Other data such as the animal entry order and the purchase price of the bulls was collected during the auction. A linear generalized model was used to evaluate the interaction of each variable with the purchase price of the bulls. An ANOVA with Tukey post-hoc was used to compare the differences between the categories that influenced the purchase price of bulls and were realized in the software SPSS 20.0. All breeds presented declining prices from the first to the second entry order and increasing purchase prices from the order third to forth. Bulls with large frame received higher purchase prices independent of the auction order, except for the second order of entry, in which medium and small animals were more valued. Angus bulls obtained the highest prices in relation to the breeds Brangus and Hereford. The frame and breed constituted the main phenotypic characteristics that influence in price. In addition, the order of entry of bulls in the ring influence the purchase price

ATLANTIC BIRD TRAITS: a data set of bird morphological traits from the Atlantic forests of South America

Scientists have long been trying to understand why the Neotropical region holds the highest diversity of birds on Earth. Recently, there has been increased interest in morphological variation between and within species, and in how climate, topography, and anthropogenic pressures may explain and affect phenotypic variation. Because morphological data are not always available for many species at the local or regional scale, we are limited in our understanding of intra- and interspecies spatial morphological variation. Here, we present the ATLANTIC BIRD TRAITS, a data set that includes measurements of up to 44 morphological traits in 67,197 bird records from 2,790 populations distributed throughout the Atlantic forests of South America. This data set comprises information, compiled over two centuries (1820–2018), for 711 bird species, which represent 80% of all known bird diversity in the Atlantic Forest. Among the most commonly reported traits are sex (n = 65,717), age (n = 63,852), body mass (n = 58,768), flight molt presence (n = 44,941), molt presence (n = 44,847), body molt presence (n = 44,606), tail length (n = 43,005), reproductive stage (n = 42,588), bill length (n = 37,409), body length (n = 28,394), right wing length (n = 21,950), tarsus length (n = 20,342), and wing length (n = 18,071). The most frequently recorded species are Chiroxiphia caudata (n = 1,837), Turdus albicollis (n = 1,658), Trichothraupis melanops (n = 1,468), Turdus leucomelas (n = 1,436), and Basileuterus culicivorus (n = 1,384). The species recorded in the greatest number of sampling localities are Basileuterus culicivorus (n = 243), Trichothraupis melanops (n = 242), Chiroxiphia caudata (n = 210), Platyrinchus mystaceus (n = 208), and Turdus rufiventris (n = 191). ATLANTIC BIRD TRAITS (ABT) is the most comprehensive data set on measurements of bird morphological traits found in a biodiversity hotspot; it provides data for basic and applied research at multiple scales, from individual to community, and from the local to the macroecological perspectives. No copyright or proprietary restrictions are associated with the use of this data set. Please cite this data paper when the data are used in publications or teaching and educational activities. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

ATLANTIC BIRD TRAITS

Scientists have long been trying to understand why the Neotropical region holds the highest diversity of birds on Earth. Recently, there has been increased interest in morphological variation between and within species, and in how climate, topography, and anthropogenic pressures may explain and affect phenotypic variation. Because morphological data are not always available for many species at the local or regional scale, we are limited in our understanding of intra- and interspecies spatial morphological variation. Here, we present the ATLANTIC BIRD TRAITS, a data set that includes measurements of up to 44 morphological traits in 67,197 bird records from 2,790 populations distributed throughout the Atlantic forests of South America. This data set comprises information, compiled over two centuries (1820–2018), for 711 bird species, which represent 80% of all known bird diversity in the Atlantic Forest. Among the most commonly reported traits are sex (n = 65,717), age (n = 63,852), body mass (n = 58,768), flight molt presence (n = 44,941), molt presence (n = 44,847), body molt presence (n = 44,606), tail length (n = 43,005), reproductive stage (n = 42,588), bill length (n = 37,409), body length (n = 28,394), right wing length (n = 21,950), tarsus length (n = 20,342), and wing length (n = 18,071). The most frequently recorded species are Chiroxiphia caudata (n = 1,837), Turdus albicollis (n = 1,658), Trichothraupis melanops (n = 1,468), Turdus leucomelas (n = 1,436), and Basileuterus culicivorus (n = 1,384). The species recorded in the greatest number of sampling localities are Basileuterus culicivorus (n = 243), Trichothraupis melanops (n = 242), Chiroxiphia caudata (n = 210), Platyrinchus mystaceus (n = 208), and Turdus rufiventris (n = 191). ATLANTIC BIRD TRAITS (ABT) is the most comprehensive data set on measurements of bird morphological traits found in a biodiversity hotspot; it provides data for basic and applied research at multiple scales, from individual to community, and from the local to the macroecological perspectives. No copyright or proprietary restrictions are associated with the use of this data set. Please cite this data paper when the data are used in publications or teaching and educational activities. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

ATLANTIC ANTS: a data set of ants in Atlantic Forests of South America

International audienc