White Paper: Certification, Credentials, and Credentialing in Pharmacy

The rapid evolution of the health care system has provided the pharmacy profession with opportunities to expand existing and develop new direct patient care roles. Two critical challenges that the pharmacy profession must contend with to ensure the continued expansion and acceptance of pharmacists in advanced practice roles (e.g. direct patient care roles) are: (1) To develop and implement a credible, systematic, standards-based, and profession-wide plan that includes a system for identifying the types of pharmacists\u27 practices requiring credentials, a validated certification process, and a computerized credentialing process that is current, comprehensive, and easily accessible; (2) To establish the credibility of these advanced practice credentials and the certification and credentialing processes with other health care providers, the public, employers and payers (government and commercial)

Plume Dispersion in Four Pine Thinning Scenarios: Development of a Simple Pheromone Dispersion Model

A unique field campaign was conducted in 2004 to examine how changes in stand density may affect dispersion of insect pheromones in forest canopies. Over a 14-day period, 126 tracer tests were performed, and conditions ranged from an unthinned loblolly pine (Pinus taeda) canopy through a series of thinning scenarios with basal areas of 32.1, 23.0, and 16.1 m2ha-1. In this paper, one case study was used to visualize the nature of winds and plume diffusion. Also, a simple empirical model was developed to estimate maximum average concentration as a function of downwind distance, travel time, wind speed, and turbulence statistics at the source location. Predicted concentrations from the model were within a factor of 3 for 82.1 percent and 88.1 percent of the observed concentrations at downwind distances of 5 and 10 m, respectively. In addition, the model was used to generate a field chart to predict optimum spacing in arrays of anti-aggregation pheromone dispensers

A Simple Model to Predict Scalar Dispersion within a Successively Thinned Loblolly Pine Canopy

Bark beetles kill millions of acres of trees in the United States annually by using chemical signaling to attack host trees en masse. As an attempt to control infestations, forest managers use synthetic semiochemical sources to attract beetles to traps and/or repel beetles from high-value resources such as trees and stands. The purpose of this study was to develop a simple numerical technique that may be used by forest managers as a guide in the placement of synthetic semiochemicals. The authors used a one-dimensional, one-equation turbulence model (k–lm) to drive a three-dimensional transport and dispersion model. Predictions were compared with observations from a unique tracer gas experiment conducted in a successively thinned loblolly pine canopy. Predictions of wind speed and turbulent kinetic energy compared well with observations. Scalar concentration was predicted well and trends of maximum observed concentration versus leaf area index were captured within 30 m of the release location. A hypothetical application of the numerical technique was conducted for a 12-day period to demonstrate the model’s usefulness to forest managers

A Simple Model to Predict Scalar Dispersion within a Successively Thinned Loblolly Pine Canopy

Bark beetles kill millions of acres of trees in the United States annually by using chemical signaling to attack host trees en masse. As an attempt to control infestations, forest managers use synthetic semiochemical sources to attract beetles to traps and/or repel beetles from high-value resources such as trees and stands. The purpose of this study was to develop a simple numerical technique that may be used by forest managers as a guide in the placement of synthetic semiochemicals. The authors used a one-dimensional, one-equation turbulence model (k–lm) to drive a three-dimensional transport and dispersion model. Predictions were compared with observations from a unique tracer gas experiment conducted in a successively thinned loblolly pine canopy. Predictions of wind speed and turbulent kinetic energy compared well with observations. Scalar concentration was predicted well and trends of maximum observed concentration versus leaf area index were captured within 30 m of the release location. A hypothetical application of the numerical technique was conducted for a 12-day period to demonstrate the model’s usefulness to forest managers

Parkinsons disease variant detection and disclosure: PD GENEration, a North American study.

Variants in seven genes (LRRK2, GBA1, PRKN, SNCA, PINK1, PARK7 and VPS35) have been formally adjudicated as causal contributors to Parkinsons disease; however, individuals with Parkinsons disease are often unaware of their genetic status since clinical testing is infrequently offered. As a result, genetic information is not incorporated into clinical care, and variant-targeted precision medicine trials struggle to enrol people with Parkinsons disease. Understanding the yield of genetic testing using an established gene panel in a large, geographically diverse North American population would help patients, clinicians, clinical researchers, laboratories and insurers better understand the importance of genetics in approaching Parkinsons disease. PD GENEration is an ongoing multi-centre, observational study (NCT04057794, NCT04994015) offering genetic testing with results disclosure and genetic counselling to those in the US (including Puerto Rico), Canada and the Dominican Republic, through local clinical sites or remotely through self-enrolment. DNA samples are analysed by next-generation sequencing including deletion/duplication analysis (Fulgent Genetics) with targeted testing of seven major Parkinsons disease-related genes. Variants classified as pathogenic/likely pathogenic/risk variants are disclosed to all tested participants by either neurologists or genetic counsellors. Demographic and clinical features are collected at baseline visits. Between September 2019 and June 2023, the study enrolled 10 510 participants across >85 centres, with 8301 having received results. Participants were: 59% male; 86% White, 2% Asian, 4% Black/African American, 9% Hispanic/Latino; mean age 67.4 ± 10.8 years. Reportable genetic variants were observed in 13% of all participants, including 18% of participants with one or more high risk factors for a genetic aetiology: early onset (<50 years), high-risk ancestry (Ashkenazi Jewish/Basque/North African Berber), an affected first-degree relative; and, importantly, in 9.1% of people with none of these risk factors. Reportable variants in GBA1 were identified in 7.7% of all participants; 2.4% in LRRK2; 2.1% in PRKN; 0.1% in SNCA; and 0.2% in PINK1, PARK7 or VPS35 combined. Variants in more than one of the seven genes were identified in 0.4% of participants. Approximately 13% of study participants had a reportable genetic variant, with a 9% yield in people with no high-risk factors. This supports the promotion of universal access to genetic testing for Parkinsons disease, as well as therapeutic trials for GBA1 and LRRK2-related Parkinsons disease

Erratum to: Methods for evaluating medical tests and biomarkers

[This corrects the article DOI: 10.1186/s41512-016-0001-y.]

Meta-analysis of genome-wide association studies discovers multiple loci for chronic lymphocytic leukemia.

Chronic lymphocytic leukemia (CLL) is a common lymphoid malignancy with strong heritability. To further understand the genetic susceptibility for CLL and identify common loci associated with risk, we conducted a meta-analysis of four genome-wide association studies (GWAS) composed of 3,100 cases and 7,667 controls with follow-up replication in 1,958 cases and 5,530 controls. Here we report three new loci at 3p24.1 (rs9880772, EOMES, P=2.55 × 10(-11)), 6p25.2 (rs73718779, SERPINB6, P=1.97 × 10(-8)) and 3q28 (rs9815073, LPP, P=3.62 × 10(-8)), as well as a new independent SNP at the known 2q13 locus (rs9308731, BCL2L11, P=1.00 × 10(-11)) in the combined analysis. We find suggestive evidence (P<5 × 10(-7)) for two additional new loci at 4q24 (rs10028805, BANK1, P=7.19 × 10(-8)) and 3p22.2 (rs1274963, CSRNP1, P=2.12 × 10(-7)). Pathway analyses of new and known CLL loci consistently show a strong role for apoptosis, providing further evidence for the importance of this biological pathway in CLL susceptibility

Histone H3.3 beyond cancer: Germline mutations in Histone 3 Family 3A and 3B cause a previously unidentified neurodegenerative disorder in 46 patients

Although somatic mutations in Histone 3.3 (H3.3) are well-studied drivers of oncogenesis, the role of germline mutations remains unreported. We analyze 46 patients bearing de novo germline mutations in histone 3 family 3A (H3F3A) or H3F3B with progressive neurologic dysfunction and congenital anomalies without malignancies. Molecular modeling of all 37 variants demonstrated clear disruptions in interactions with DNA, other histones, and histone chaperone proteins. Patient histone posttranslational modifications (PTMs) analysis revealed notably aberrant local PTM patterns distinct from the somatic lysine mutations that cause global PTM dysregulation. RNA sequencing on patient cells demonstrated up-regulated gene expression related to mitosis and cell division, and cellular assays confirmed an increased proliferative capacity. A zebrafish model showed craniofacial anomalies and a defect in Foxd3-derived glia. These data suggest that the mechanism of germline mutations are distinct from cancer-associated somatic histone mutations but may converge on control of cell proliferation