Population fluctuation of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and survey of some natural enemies in Ecuador

Diaphorina citri detected in Ecuador for the first time in 2013, is an insect pest of global relevance. It is the main vector of the phloem-limited bacterium Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing (HLB), a devastating disease of citrus trees. During the period from April 2016 - July 2017, population fluctuation studies and a search for natural enemies of D. citri were conducted on young shoots of orange jessamine, Murraya paniculata, and Citrus spp. The natural enemies of D. citri were collected in the Provinces of Guayas and Santa Elena, Ecuador. A total of 1660 specimens of predators belonging to five species were collected, consisting of three coccinellids, Cheilomenes sexmaculata, the most abundant species (39.9%, P <0.05), followed by Cycloneda sanguinea (15.8%), and Paraneda pallidula guticollis (4.1%) (Coleoptera: Coccinellidae), an assassin bug, Zelus sp. (Hemiptera: Reduviidae) (17.8%), and the lacewing Ceraeochrysa sp. (Neuroptera: Chrysopidae) (22.4%). The parasitoid wasp Tamarixia radiata also was found, reaching a parasitization rate of 90% of the psyllid nymphs. This study represents the first report of P. pallidula guticollis feeding on D. citri. The diversity of natural enemies and the high level of parasitism detected suggest the importance of natural enemies as biological control agents of this important phytophagous insect pest. The natural control by these beneficial insects would make unnecessary the applications of chemical insecticides that are carried out in Ecuador for the control of this insect, especially at this moment when the HLB causing bacterium has not been reported in Ecuador.Diaphorina citri, especie detectada en Ecuador por primera vez en 2013, es una plaga de relevancia mundial debido a que es el principal vector de la bacteria Candidatus Liberibacter asiaticus, agente causal del Huanglongbing (HLB), una devastadora enfermedad de los cítricos. Durante el período abril 2016 – julio 2017, se realizaron estudios de fluctuación poblacional e inventario de enemigos naturales de D. citri en brotes jóvenes de azahar de la India, Murraya paniculata y citricos, Citrus spp. Los enemigos naturales fueron colectados en las provincias de Guayas y Santa Elena, Ecuador. Se colectaron un total de 1660 especímenes pertenecientes a cinco especies, entre estos, tres coccinélidos, Cheilomenes sexmaculata, la especie más abundante (39,9%, P<0,05), seguido por Cycloneda sanguinea (15,8%) y Paraneda pallidula guticollis 4,1% (Coleoptera: Coccinellidae), una chinche depredadora, Zelus sp. (Hemiptera: Reduviidae) (17,8%) y la crisopa Ceraeochrysa sp. (Neuroptera: Chrysopidae) (22,4%). La avispita parasítica Tamarixia radiata también fue encontrada, alcanzando un porcentaje de parasitismo del 90% en las ninfas de psílido. Este estudio representa el primer reporte de P. pallidula guticollis alimentándose de D. citri. La diversidad de enemigos naturales y el alto nivel de parasitismo detectado sugieren la importancia de los enemigos naturales como agentes de control biológico de esta importante plaga. El control natural existente haría innecesarias las aplicaciones de insecticidas químicos que se llevan a cabo en el Ecuador para el control de este insecto, especialmente en este momento que el HLB no se ha encontrado en Ecuador.Gerencia de Comunicación Institucional, DG SICyP, INTAFil: Chavez, Y. Ministerio de Agricultura y Ganadería; EcuadorFil: Castro, C. Investigador independiente; EcuadorFil: González, Guillermo F. Investigador independiente; ChileFil: Castro, Jessenia. Universidad Técnica de Manabí. Facultad de Ingeniería Agronómica; EcuadorFil: Peñarrieta Bravo, Soraya. Universidad Técnica de Manabí. Facultad de Ingeniería Agronómica; EcuadorFil: Perez-Almeida, Iris. Universidad ECOTEC. Facultad de Ingenierías. Carrera de Ingeniería Agrónoma; EcuadorFil: Chirinos, Dorys Terezinha. Universidad Técnica de Manabí. Facultad de Ingeniería Agronómica; EcuadorFil: Kondo Rodríguez, Demian Takumasa. Corporación colombiana de investigación agropecuaria AGROSAVIA. Centro de Investigación Palmira; Colombi

Detection of early Alzheimer's disease in MCI patients by the combination of MMSE and an episodic memory test

BACKGROUND: Mild cognitive impairment (MCI) is a heterogeneous clinical entity that comprises the prodromal phase of Alzheimer's disease (Pr-AD). New biomarkers are useful in detecting Pr-AD, but they are not universally available. We aimed to investigate baseline clinical and neuropsychological variables that might predict progression from MCI to AD dementia. METHODS: All patients underwent a complete clinical and neuropsychological evaluation at baseline and every 6 months during a two-year follow-up period, with 54 out of 109 MCI patients progressing to dementia (50 of them progressed to AD dementia), and 55 remaining as stable MCI (S-MCI). RESULTS: A combination of MMSE and California Verbal Learning Test Long Delayed Total Recall (CVLT-LDTR) constituted the best predictive model: subjects scoring above 26/30 on MMSE and 4/16 on CVLT-LDTR had a negative predictive value of 93.93% at 2 years, whereas those subjects scoring below both of these cut-off scores had a positive predictive value of 80.95%. CONCLUSIONS: Pr-AD might be distinguished from S-MCI at baseline using the combination of MMSE and CVLT-LDTR. These two neuropsychological predictors are relatively brief and may be readily completed in non-specialist clinical settings

Shared Oncogenic Pathways Implicated in Both Virus-Positive and UV-Induced Merkel Cell Carcinomas

Merkel cell carcinoma (MCC) is a highly malignant neuroendocrine tumor of the skin whose molecular pathogenesis is not completely understood, despite the role that Merkel cell polyomavirus can play in 55e90% of cases. To study potential mechanisms driving this disease in clinically characterized cases, we searched for somatic mutations using whole-exome sequencing, and extrapolated our findings to study functional biomarkers reporting on the activity of the mutated pathways. Confirming previous results, Merkel cell polyomavirus-negative tumors had higher mutational loads with UV signatures and more frequent mutations in TP53 and RB compared with their Merkel cell polyomavirus-positive counterparts. Despite important genetic differences, the two Merkel cell carcinoma etiologies both exhibited nuclear accumulation of oncogenic transcription factors such as NFAT or nuclear factor of activated T cells (NFAT), P-CREB, and P-STAT3, indicating commonly deregulated pathogenic mechanisms with the potential to serve as targets for therapy. A multivariable analysis identified phosphorylated CRE-binding protein as an independent survival factor with respect to clinical variables and Merkel cell polyomavirus status in our cohort of Merkel cell carcinoma patients.This work was supported by grants from Instituto de Salud-Carlos III (ISCIII); cofinanced by the European Union; (FEDER) (PI12/00357), and a Ramón and Cajal research program (MINECO; RYC-2013-14097) to JPV, Asociación Española Contra el Cáncer and ISCIII grants (RD06/0020/0107, RD012/0036/0060) to MAP, and Coordinated Project of Excellence inter-Institutos de investigación acreditados institutes (ISCIII; PIE15/00081) to MAP. The Ramón and Cajal research program also supports IV. SD was supported by the Torres Quevedo subprogram (MICINN; PTQ-12-05391)

Novel genes and sex differences in COVID-19 severity

[EN] Here, we describe the results of a genome-wide study conducted in 11 939 coronavirus disease 2019 (COVID-19) positive cases with an extensive clinical information that were recruited from 34 hospitals across Spain (SCOURGE consortium). In sex-disaggregated genome-wide association studies for COVID-19 hospitalization, genome-wide significance (P < 5 × 10−8) was crossed for variants in 3p21.31 and 21q22.11 loci only among males (P = 1.3 × 10−22 and P = 8.1 × 10−12, respectively), and for variants in 9q21.32 near TLE1 only among females (P = 4.4 × 10−8). In a second phase, results were combined with an independent Spanish cohort (1598 COVID-19 cases and 1068 population controls), revealing in the overall analysis two novel risk loci in 9p13.3 and 19q13.12, with fine-mapping prioritized variants functionally associated with AQP3 (P = 2.7 × 10−8) and ARHGAP33 (P = 1.3 × 10−8), respectively. The meta-analysis of both phases with four European studies stratified by sex from the Host Genetics Initiative (HGI) confirmed the association of the 3p21.31 and 21q22.11 loci predominantly in males and replicated a recently reported variant in 11p13 (ELF5, P = 4.1 × 10−8). Six of the COVID-19 HGI discovered loci were replicated and an HGI-based genetic risk score predicted the severity strata in SCOURGE. We also found more SNP-heritability and larger heritability differences by age (<60 or ≥60 years) among males than among females. Parallel genome-wide screening of inbreeding depression in SCOURGE also showed an effect of homozygosity in COVID-19 hospitalization and severity and this effect was stronger among older males. In summary, new candidate genes for COVID-19 severity and evidence supporting genetic disparities among sexes are provided.S

Diffusion of the results of the quality learning processes in the Faculty of Fine Arts. Physical and digital creation and exhibition as an essential part of the training process

La difusión de las producciones creativas de la comunidad de la Facultad de Bellas Artes a través de proyectos expositivos y plataformas digitales es una herramienta para el incremento de calidad que contribuye a reforzar la marca Complutense.The diffusion of the creative productions of the community of the Faculty of Fine Arts through exhibition projects and digital platforms is a tool for increasing quality that contributes to strengthening the Complutense brand.Fac. de Bellas ArtesFALSEsubmitte

Serigrafía Inquieta

Un proyecto de Aprendizaje y Servicio de la UCM, cuyo título original de Serigrafía Inquieta dio paso al definitivo de Serigrafía Ambulante de Aluche. -Facultad de Bellas Artes: Técnicas de estampación, Diseño, Escultura. -Asociación de Vecinos de Aluche (AVA): JUBA, LA CABA.Serigrafía Ambulante de Aluche describe una modalidad técnica y un ámbito de trabajo. Su capacidad de estampar sin complementos mecánicos proporciona a la serigrafía la posibilidad de desplazarse libremente fuera del taller, profundizando en el desborde de la gráfica, ya no en manos de unas capacidades depositadas en la obra gráfica y su discurso, sino además en la posibilidad que ofrece la exposición al elemento procedimental. Exploramos las posibilidades de la estampación ambulante (a bordo de un remolque de bicicleta), desde el apoyo logístico establecido en el taller de la Facultad de Bellas Artes (UCM) y en la Asociación de Vecinos de Aluche (AVA), en dos facetas derivadas de su condición ambulante, como son la instalación de eventuales talleres itinerantes de estampación, y la estampación callejera. Como estampación callejera acudimos a eventos en calidad de convocados por parte de la asociación vecinal o del estamento estudiantil. El colectivo en su conjunto decide dónde y cómo llevar a cabo una intervención en el espacio público, proceso que comienza con el desarrollo de unas gráficas, en base a unas consignas determinadas, la preparación de matrices, y el traslado del material al local del AVA como base de operaciones, para finalmente acudir a estampar durante el transcurso del evento. Participamos como agitadores estampando y repartiendo material gráfico alusivo, pero abarcando también todo cuanto tenga que ver con la cartelería como herramienta promocional. Consideramos de gran importancia el trabajo de base junto a otras asociaciones del ámbito juvenil y educativo. Así, la estampación in situ como experiencia, donde participamos todos, no hace más que culminar un proceso que incluye el aporte de las competencias implicadas en el proyecto, como el trabajo sobre el sentido comunicacional del diseño gráfico y la preparación de herramientas para la estampación.Depto. de Dibujo y GrabadoFac. de Bellas ArtesFALSEunpu

Novel genes and sex differences in COVID-19 severity.

Here we describe the results of a genome-wide study conducted in 11 939 COVID-19 positive cases with an extensive clinical information that were recruited from 34 hospitals across Spain (SCOURGE consortium). In sex-disaggregated genome-wide association studies for COVID-19 hospitalization, genome-wide significance (p < 5x10-8) was crossed for variants in 3p21.31 and 21q22.11 loci only among males (p = 1.3x10-22 and p = 8.1x10-12, respectively), and for variants in 9q21.32 near TLE1 only among females (p = 4.4x10-8). In a second phase, results were combined with an independent Spanish cohort (1598 COVID-19 cases and 1068 population controls), revealing in the overall analysis two novel risk loci in 9p13.3 and 19q13.12, with fine-mapping prioritized variants functionally associated with AQP3 (p = 2.7x10-8) and ARHGAP33 (p = 1.3x10-8), respectively. The meta-analysis of both phases with four European studies stratified by sex from the Host Genetics Initiative confirmed the association of the 3p21.31 and 21q22.11 loci predominantly in males and replicated a recently reported variant in 11p13 (ELF5, p = 4.1x10-8). Six of the COVID-19 HGI discovered loci were replicated and an HGI-based genetic risk score predicted the severity strata in SCOURGE. We also found more SNP-heritability and larger heritability differences by age (<60 or ≥ 60 years) among males than among females. Parallel genome-wide screening of inbreeding depression in SCOURGE also showed an effect of homozygosity in COVID-19 hospitalization and severity and this effect was stronger among older males. In summary, new candidate genes for COVID-19 severity and evidence supporting genetic disparities among sexes are provided