INQUIRE: a case study in evaluating the potential of online MCQ tests in a discursive subject

There has been a wealth of investigation into the use of online multiple-choice questions as a means of summative assessment, however the research into the use of formative MCQs by the same mode of delivery still remains patchy. Similarly, research and implementation has been largely concentrated within the Sciences and Medicine rather than the more discursive subjects within the Humanities and Social Sciences. The INQUIRE (Interactive Questions Reinforcing Education) Evaluation Project was jointly conducted by two groups at the University of Oxford-the Said Business School and the Academic Computing Development Team to evaluate the use of online MCQs as a mechanism to reinforce and extend student learning. This initial study used a small set of highly focused MCQ tests that were designed to complement an introductory series of first-year undergraduate management lectures. MCQ is a simple and well-established technology, and hence the emphasis was very much on situating the tests within the student experience. The paper will cover how the online MCQs are intended to fit into the Oxford Undergraduate study agenda, and how a simple evaluation was executed and planned to investigate their usage and impact. The chosen method of evaluation was to combine focus groups with automated online methods of tracking, and the paper discusses the findings of both of these

Functional and structural studies of the hydrophilic acylated surface proteins from Leishmania donovani

Protozoan parasites of the genus Leishmania cause a diverse range of tropical diseases referred to as Leishmaniasis that lack effective treatment or licensed vaccines. The hydrophilic acylated surface proteins (HASPs) are present in all human infective Leishmania species, are highly immunogenic and their expression is stage-regulated during human infection. HASPs share highly conserved N- and C-terminal domains, but a subset, the HASPBs, have a divergent central domain containing extensive hydrophilic amino acid repeats that vary in number and composition, both within and between Leishmania species. HASPs associate with the membrane via an N-terminal dual acylation motif that is myristoylated and palmitoylated. The focus of this project was the HASPs from Leishmania donovani. Extensive crystallisation screening of the proteins in combination with an array of directed crystallisation strategies did not produce crystals. Biochemical and biophysical characterisation supported bioinformatic predicted intrinsic disorder. NMR analysis of HASPA was performed, including resonance assignment of 94% of the backbone nuclei. This required implementation of unlabelling protocols to resolve problems associated with spectral congestion and low sequence complexity. HN, N, Cα and Cβ chemical shift analysis revealed that HASPA does not contain elements of structural propensity. Myristoylation of the HASPs is catalysed by N-Myristoyltransferase (NMT). It was shown for the first time that recombinant NMT is able to catalyse recombinant HASP myristoylation in vitro. A fluorescence based assay, where a fluorescent CPM-CoA adduct is formed, was implemented to establish the kinetic parameters for this reaction. HASPA myristoylation was also monitored by real-time NMR spectroscopy, which revealed that the residues proximal to the N-terminus experienced the most pronounced changes in chemical shift or resonance intensity. A 2.45 Å resolution crystal structure of Leishmania major NMT in complex with 2-oxopentadecyl-CoA was elucidated. The data presented here, particularly IDP classification, will contribute to deciphering the functional role of HASPs

Embedding equality, diversity and inclusion in usability testing: recommendations and a research agenda

Technologies support our everyday lives, and to ensure that people are not routinely excluded they must be usable by the wider population. However, technologies are not commonly tested with participants from a range of backgrounds. This paper reports on interviews and roundtable discussions with people whose identities can be underrepresented in usability testing and usability researchers to discuss how equality, diversity and inclusion (EDI) can be embedded in usability testing.Key findings include (1) when people participate in research they need a sense of value, trust and agency, and (2) challenges for researchers for embedding EDI in usability testing include organisational pressures, stakeholder culture, getting guidance and recruiting who you need. Recommendations are made to researchers, and to the organisations that employ them. Additionally, we propose a research agenda for a community of users, creators of services and products, usability researchers, and all those advocating for EDI in usability research

Gender Differences in Compensation, Job Satisfaction and Other Practice Patterns in Urology

The proportion of women in urology has increased from <0.5% in 1981 to 10% today. Furthermore, 33% of students matching in urology are now female. This analysis sought to characterize the female workforce in urology in comparison to men with regard to income, workload, and job satisfaction

Identification of a novel BET bromodomain inhibitor-sensitive, gene regulatory circuit that controls Rituximab response and tumour growth in aggressive lymphoid cancers.: CYCLON-induced Rituximab resistance

International audienceImmuno-chemotherapy elicit high response rates in B-cell non-Hodgkin lymphoma but heterogeneity in response duration is observed, with some patients achieving cure and others showing refractory disease or relapse. Using a transcriptome-powered targeted proteomics screen, we discovered a gene regulatory circuit involving the nuclear factor CYCLON which characterizes aggressive disease and resistance to the anti-CD20 monoclonal antibody, Rituximab, in high-risk B-cell lymphoma. CYCLON knockdown was found to inhibit the aggressivity of MYC-overexpressing tumours in mice and to modulate gene expression programs of biological relevance to lymphoma. Furthermore, CYCLON knockdown increased the sensitivity of human lymphoma B cells to Rituximab in vitro and in vivo. Strikingly, this effect could be mimicked by in vitro treatment of lymphoma B cells with a small molecule inhibitor for BET bromodomain proteins (JQ1). In summary, this work has identified CYCLON as a new MYC cooperating factor that autonomously drives aggressive tumour growth and Rituximab resistance in lymphoma. This resistance mechanism is amenable to next-generation epigenetic therapy by BET bromodomain inhibition, thereby providing a new combination therapy rationale for high-risk lymphoma

Efficient generation of CD34+ progenitorâ derived dendritic cells from Gâ CSFâ mobilized peripheral mononuclear cells does not require hematopoietic stem cell enrichment

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141647/1/jlb0957.pd

The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency

Human immunodeficiency syndrome with loss of DCs, monocytes, and T reg cells; preservation of Langerhans cells; associated loss of BM multilymphoid progenitors; and overproduction of Flt3 ligand

New and recurrent gain-of-function STAT1 mutations in patients with chronic mucocutaneous candidiasis from Eastern and Central Europe

Background: Chronic mucocutaneous candidiasis disease (CMCD) may result from various inborn errors of interleukin (IL)-17-mediated immunity. Twelve of the 13 causal mutations described to date affect the coiled-coil domain (CCD) of STAT1. Several mutations, including R274W in particular, are recurrent, but the underlying mechanism is unclear. Objective: To investigate and describe nine patients with CMCD in Eastern and Central Europe, to assess the biochemical impact of STAT1 mutations, to determine cytokines in supernatants of Candida-exposed blood cells, to determine IL-17-producing T cell subsets and to determine STAT1 haplotypes in a family with the c.820C>T (R274W) mutation. Results: The novel c.537C>A (N179K) STAT1 mutation was gain-of-function (GOF) for γ-activated factor (GAF)-dependent cellular responses. In a Russian patient, the cause of CMCD was the newly identified c.854 A>G (Q285R) STAT1 mutation, which was also GOF for GAF-dependent responses. The c.1154C>T (T385M) mutation affecting the DNA-binding domain (DBD) resulted in a gain of STAT1 phosphorylation in a Ukrainian patient. Impaired Candida-induced IL-17A and IL-22 secretion by leucocytes and lower levels of intracellular IL-17 and IL-22 production by T cells were found in several patients. Haplotype studies indicated that the c.820C>T (R274W) mutation was recurrent due to a hotspot rather than a founder effect. Severe clinical phenotypes, including intracranial aneurysm, are presented. Conclusions: The c.537C>A and c.854A>G mutations affecting the CCD and the c.1154C>T mutation affecting the DBD of STAT1 are GOF. The c.820C>T mutation of STAT1 in patients with CMCD is recurrent due to a hotspot. Patients carrying GOF mutations of STAT1 may develop multiple intracranial aneurysms by hitherto unknown mechanisms