A study of changes in cognitive structure and measures of adjustment among members of a rehabilitation living unit

Call number: LD2668 .T4 1967 S349Master of Scienc

Method of isolating an analyte using a solid phase extraction medium

A functionalized macroporous poly(styrene divinylbenzene) particle comprises at least one ionic functional group covalently bonded thereto, the functionalized particle having sorptive capability towards an analyte, said functional group being present in the range of 0.1 to 2.5 milliequivalents per gram of poly(styrene divinylbenzene). The functionalized particles can be used in a packed column or enmeshed in a nonwoven web for utility in solid phase extraction applications

Transport into mitochondria and intramitochondrial sorting of the Fe/S protein of ubiquinol-cytochrome c reductase

The Fe/S protein of complex III is encoded by a nuclear gene, synthesized in the cytoplasm as a precursor with a 32 residue amino-terminal extension, and transported to the outer surface of the inner mitochondrial membrane. Our data suggest the following transport pathway. First, the precursor is translocated via translocation contact sites into the matrix. There, cleavage to an intermediate containing an eight residue extension occurs. The intermediate is then redirected across the inner membrane, processed to the mature subunit, and assembled into complex III. We suggest that the folding and membrane-translocation pathway in the endosymbiotic ancestor of mitochondria has been conserved during evolution of eukaryotic cells; transfer of the gene for Fe/S protein to the nucleus has led to addition of the presequence, which routes the precursor back to its “ancestral” assembly pathway

Have the roles of two functional polymorphisms in breast cancer, R72P in P53 and MDM2-309 in MDM2, become clearer?

Genetic differences between individuals have been predicted to account for disparate outcomes in patients diagnosed with cancer. The search for genetic determinants has been ongoing for a considerable amount of time and it is only now that insights have been gained into which polymorphisms are most likely to be important in determining not only disease likelihood but also outcome. The quest to be able to accurately predict patient outcomes in breast cancer may now be a step closer as increased sample size is leading to more robust statistical analysis and a better understanding of molecular mechanisms of disease are forthcoming

Simultaneous X-ray and UV spectroscopy of the Seyfert 1 galaxy NGC 5548.II. Physical conditions in the X-ray absorber

We present the results from a 500 ks Chandra observation of the Seyfert 1 galaxy NGC 5548. We detect broadened emission lines of O VII and C VI in the spectra, similar to those observed in the optical and UV bands. The source was continuously variable, with a 30 % increase in luminosity in the second half of the observation. No variability in the warm absorber was detected between the spectra from the first 170 ks and the second part of the observation. The velocity structure of the X-ray absorber is consistent with the velocity structure measured simultaneously in the ultraviolet spectra. We find that the highest velocity outflow component, at -1040 km/s, becomes increasingly important for higher ionization parameters. This velocity component spans at least three orders of magnitude in ionization parameter, producing both highly ionized X-ray absorption lines (Mg XII, Si XIV) as well as UV absorption lines. A similar conclusion is very probable for the other four velocity components. Based upon our observations, we argue that the warm absorber probably does not manifest itself in the form of photoionized clumps in pressure equilibrium with a surrounding wind. Instead, a model with a continuous distribution of column density versus ionization parameter gives an excellent fit to our data. From the shape of this distribution and the assumption that the mass loss through the wind should be smaller than the accretion rate onto the black hole, we derive upper limits to the solid angle as small as 10^{-4} sr. From this we argue that the outflow occurs in density-stratified streamers. The density stratification across the stream then produces the wide range of ionization parameter observed in this source. Abridged.Comment: 21 pages, 12 figures accepted for publication in A&

Biomechanical evolution of solid bones in large animals: a microanatomical investigation

International audienc

Import of cytochrome c into mitochondria

The import of cytochrome c into mitochondria can be resolved into a number of discrete steps. Here we report on the covalent attachment of heme to apocytochrome c by the enzyme cytochrome c heme lyase in mitochondria from Neurospora crassa. A new method was developed to measure directly the linkage of heme to apocytochrome c. This method is independent of conformational changes in the protein accompanying heme attachment. Tryptic peptides of [35S]cysteine-labelled apocytochrome c, and of enzymatically formed holocytochrome c, were resolved by reverse-phase HPLC. The cysteine-containing peptide to which heme was attached eluted later than the corresponding peptide from apocytochrome c and could be quantified by counting 35S radioactivity as a measure of holocytochrome c formation. Using this procedure, the covalent attachment of heme to apocytochrome c, which is dependent on the enzyme cytochrome c heme lyase, could be measured. Activity required heme (as hemin) and could be reversibly inhibited by the analogue deuterohemin. Holocytochrome c formation was stimulated 5–10-fold by NADH > NADPH > glutathione and was independent of a potential across the inner mitochondrial membrane. NADH was not required for the binding of apocytochrome c to mitochondria and was not involved in the reduction of the cysteine thiols prior to heme attachment. Holocytochrome c formation was also dependent on a cytosolic factor that was necessary for the heme attaching step of cytochrome c import. The factor was a heat-stable, protease-insensitive, low-molecular-mass component of unknown function. Cytochrome c heme lyase appeared to be a soluble protein located in the mitochondrial intermembrane space and was distinct from the previously identified apocytochrome c binding protein having a similar location. A model is presented in which the covalent attachment of heme by cytochrome c heme lyase also plays an essential role in the import pathway of cytochrome c

Broad-line Balmer Decrements in Blue Active Galactic Nuclei

We have investigated the broad-line Balmer decrements (Halpha/Hbeta) for a large, homogeneous sample of Seyfert 1 galaxies and QSOs using spectroscopic data obtained in the Sloan Digital Sky Survey. The sample, drawn from the Fourth Data Release, comprises 446 low redshift (z < 0.35) active galactic nuclei (AGN) that have blue optical continua as indicated by the spectral slopes in order to minimize the effect of dust extinction. We find that (i) the distribution of the intrinsic broad-line Halpha/Hbeta ratio can be well described by log-Gaussian, with a peak at Halpha/Hbeta=3.06 and a standard deviation of about 0.03 dex only; (ii) the Balmer decrement does not correlate with AGN properties such as luminosity, accretion rate, and continuum slope, etc.; (iii) on average, the Balmer decrements are found to be only slightly larger in radio-loud sources (3.37) and sources having double-peaked emission-line profiles (3.27) compared to the rest of the sample. We therefore suggest that the broad-line Halpha/Hbeta ratio can be used as a good indicator for dust extinction in the AGN broad-line region; this is especially true for radio-quiet AGN with regular emission-line profiles, which constitute the vast majority of the AGN population.Comment: To appear in MNRAS. The data and the fitted parameters for the decomposed spectral components (continuum, FeII and other emission lines) of the 446 blue AGNs are available at http://staff.ustc.edu.cn/~xbdong/Data_Release/blueAGN_DR4

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing.

Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive understanding of distinct subpopulations and their functional relevance in human satellite cells is lacking. We used a combination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically separate novel subpopulations of human PAX7+ satellite cells (Hu-MuSCs) from normal muscles. We found that, although relatively homogeneous compared to activated satellite cells and committed progenitors, the Hu-MuSC pool contains clusters of transcriptionally distinct cells with consistency across human individuals. New surface marker combinations were enriched in transcriptional subclusters, including a subpopulation of Hu-MuSCs marked by CXCR4/CD29/CD56/CAV1 (CAV1+). In vitro, CAV1+ Hu-MuSCs are morphologically distinct, and characterized by resistance to activation compared to CAV1- Hu-MuSCs. In vivo, CAV1+ Hu-MuSCs demonstrated increased engraftment after transplantation. Our findings provide a comprehensive transcriptional view of normal Hu-MuSCs and describe new heterogeneity, enabling separation of functionally distinct human satellite cell subpopulations