Imported African histoplasmosis by Histoplasma capsulatum var. duboisii in an HIV-2 infected patient

Objectives: African histoplasmosis caused by the fungus Histoplasma capsulatum var. duboisii, is a rare endemic mycosis occuring in western and central regions of sub-Saharian Africa. For unknown reasons, although HIV infection and H. capsulatum var. duboisii coexist in Africa, this coinfection remains rare. In Europe, diagnosed cases of African histoplasmosis are all imported. We describe a case of African histoplasmosis on a Portuguese war veteran co-infected with HIV-2 who fought in Guiné-Bissau in 1963-65 and Angola in 1972. Methods: We report a case of a 76-year-old man, diagnosed with HIV-2 infection in the previous year (under combined antiretroviral therapy) presenting an ulcerated skin lesion on the right tight (image 1), just above the knee. He was diagnosed pulmonary tuberculosis the year before and was finishing one year treatment. The solitary skin lesion begun as a small non-pruriginous eritematous papule, evolving in 6 month to a painless 3-4cm ulcer with raised borders surrounded by a hiperpigmented halo. There were no adenopathies or bone lesions. Respiratory samples and blood cultures were systematically negative for Histoplasma capsulatum. He was treated with IV liposomal Amphotericin-B for one month, followed by oral itraconazol (now on the first month), with a favourable clinical outcome. Results: Histopathology of skin biopsy revealed a superficial ulceration with underlying granulomatous infiltrate with many giant cells, where numerous round mononucleated yeasts measuring 7-8µm were evident and highlighted with PAS and Grocott stain (image 2, left side). Skin biopsy was observed on a wet mount with KOH and revealed numerous round yeasts that were also seen on Gram stain, measuring 7-8µm. Culture of skin biopsy on two Sabouraud dextrose agar (with and without cicloheximide) showed growth of a filamentous fungus compatible with Histoplasma capsulatum (image 2, right side), with large thick-walled spherical macroconidia with finger-like projections (tuberculate conidia) that arise from short conidiophores, and small oval microconidia arising on short stalks from undifferentiated hyphae. Reversion to the yeast fase has not been succeed yet. Identification was further confirmed by sequencing of genomic DNA fragments using the universal fungal primers ITS1 and ITS4. The sequences obtained were compared with sequences deposited in the GenBank and the result was: Histoplasma capsulatum var. duboisii (99% homology). Conclusion: With banalization of business or leisure trips, endemic mycosis are becoming frequently diagnosed in countries outside their natural geographic endemic areas and only a high index of suspicion makes the diagnosis possible. Apart from trips, nowadays in Portugal 4% of the resident population is immigrant, mostly from Brasil (25.5%) but also from Angola and Guiné-Bissau (9.2%), being the former an endemic country of American histoplasmosis and the later of both American and African histoplasmosis. We consider histoplasmosis a probable underdiagnosed disease that should be suspected mainly in immunodeficient HIV positive individuals with a past history of travel or residence in an endemic area

Search for point-like sources of ultra-high energy neutrinos at the Pierre Auger Observatory and improved limit on the diffuse flux of tau neutrinos

The Surface Detector array of the Pierre Auger Observatory can detect neutrinos with energy between 10^17 eV and 10^20 eV from point-like sources across the sky south of +55 deg and north of -65 deg declinations. A search has been performed for highly inclined extensive air showers produced by the interaction of neutrinos of all flavours in the atmosphere (downward-going neutrinos), and by the decay of tau leptons originating from tau neutrinos interactions in the Earth's crust (Earth-skimming neutrinos). No candidate neutrinos have been found in data up to 2010 May 31. This corresponds to an equivalent exposure of ~3.5 years of a full surface detector array for the Earth-skimming channel and ~2 years for the downward-going channel. An improved upper limit on the diffuse flux of tau neutrinos has been derived. Upper limits on the neutrino flux from point-like sources have been derived as a function of the source declination. Assuming a differential neutrino flux k_PS E^-2 from a point-like source, 90% C.L. upper limits for k_PS at the level of ~5 x 10^-7 and 2.5 x 10^-6 GeV cm^-2 s^-1 have been obtained over a broad range of declinations from the searches of Earth-skimming and downward-going neutrinos, respectively.Peer Reviewe

A Search for Point Sources of EeV Neutrons

A thorough search of the sky exposed at the Pierre Auger Cosmic Ray Observatory reveals no statistically significant excess of events in any small solid angle that would be indicative of a flux of neutral particles from a discrete source. The search covers from -90 to +15 degrees in declination using four different energy ranges above 1 EeV (10^18 eV). The method used in this search is more sensitive to neutrons than to photons. The upper limit on a neutron flux is derived for a dense grid of directions for each of the four energy ranges. These results constrain scenarios for the production of ultra-high energy cosmic rays in the Galaxy.Peer Reviewe

A search for anisotropy in the arrival directions of ultra high energy cosmic rays recorded at the Pierre Auger Observatory

Observations of cosmic ray arrival directions made with the Pierre Auger Observatory have previously provided evidence of anisotropy at the 99% CL using the correlation of ultra high energy cosmic rays (UHECRs) with objects drawn from the Veron-Cetty Veron catalog. In this paper we report on the use of three catalog independent methods to search for anisotropy. The 2pt-L, 2pt+ and 3pt methods, each giving a different measure of self-clustering in arrival directions, were tested on mock cosmic ray data sets to study the impacts of sample size and magnetic smearing on their results, accounting for both angular and energy resolutions. If the sources of UHECRs follow the same large scale structure as ordinary galaxies in the local Universe and if UHECRs are deflected no more than a few degrees, a study of mock maps suggests that these three methods can efficiently respond to the resulting anisotropy with a P-value = 1.0% or smaller with data sets as few as 100 events. Using data taken from January 1, 2004 to July 31, 2010 we examined the 20, 30,..., 110 highest energy events with a corresponding minimum energy threshold of about 49.3 EeV. The minimum P-values found were 13.5% using the 2pt-L method, 1.0% using the 2pt+ method and 1.1% using the 3pt method for the highest 100 energy events. In view of the multiple (correlated) scans performed on the data set, these catalog-independent methods do not yield strong evidence of anisotropy in the highest energy cosmic rays.Peer Reviewe

Highlights from the Pierre Auger Observatory

The Pierre Auger Observatory is the world’s largest cosmic ray observatory. Our current exposure reaches nearly 40,000 km$^{2}$sr and provides us with an unprecedented quality data set. The performance and stability of the detectors and their enhancements are described. Data analyses have led to a number of major breakthroughs. Among these, we discuss the energy spectrum and the searches for large-scale anisotropies. We present analyses of our X$_{max}$ data and show how it can be interpreted in terms of mass composition. We also describe some new analyses that extract mass-sensitive parameters from the 100 % duty cycle surface detector (SD) data. A coherent interpretation of all these recent results opens new directions. The consequences regarding the cosmic ray composition and the properties of ultrahigh-energy cosmic ray (UHECR) sources are briefly discussed.Peer Reviewe

Depth of maximum of air-shower profiles at the Pierre Auger Observatory. II. Composition implications

Using the data taken at the Pierre Auger Observatory between December 2004 and December 2012, we have examined the implications of the distributions of depths of atmospheric shower maximum (Xmax), using a hybrid technique, for composition and hadronic interaction models. We do this by fitting the distributions with predictions from a variety of hadronic interaction models for variations in the composition of the primary cosmic rays and examining the quality of the fit. Regardless of what interaction model is assumed, we find that our data are not well described by a mix of protons and iron nuclei over most of the energy range. Acceptable fits can be obtained when intermediate masses are included, and when this is done consistent results for the proton and iron-nuclei contributions can be found using the available models. We observe a strong energy dependence of the resulting proton fractions, and find no support from any of the models for a significant contribution from iron nuclei. However, we also observe a significant disagreement between the models with respect to the relative contributions of the intermediate components.Peer Reviewe

A model for net-baryon rapidity distribution

In nuclear collisions, a sizable fraction of the available energy is carried away by baryons. As the baryon number is conserved, the net-baryon $B-\bar{B}$ retains information on the energy-momentum carried by the incoming nuclei. A simple and consistent model for net-baryon production in high energy proton-proton and nucleus-nucleus collisions is presented. The basic ingredients of the model are valence string formation based on standard PDFs with QCD evolution and string fragmentation via the Schwinger mechanism. The results of the model are presented and compared with data at different centre-of-mass energies and centralities, as well as with existing models. These results show that a good description of the main features of net-baryon data is possible in the framework of a simplistic model, with the advantage of making the fundamental production mechanisms manifest.Comment: 9 pages, 12 figures; in fig. 11 a) the vertical scale was correcte

Efeito da desidratação sobre o valor nutricional de prunoideas

Neste trabalho utilizou-se a desidratação com o objetivo de encontrar soluções para PME frutícolas localizadas no Distrito de Castelo Branco, relativamente ao escoamento dos excedentes das produções sazonais de prunóideas e ao aproveitamento de fruta com calibre reduzido e com pouca aceitação no mercado. Foram selecionados quatro produtos (ameixas, cerejas, nectarinas e pêssegos) que, depois de lavados com água potável, descaroçados e/ou fatiados, foram colocados num desidratador com 4 m3, com capacidade para dois carros verticais de 12 tabuleiros com tapetes anti aderentes. O programa de desidratação foi o seguinte: 60°C; humidade máxima 10%; velocidade 75%; ciclo de desidratação até 26 horas. A desidratação reduziu o teor em água dos quatro frutos (83,1% para 7,4%) e aw (0,968 para 0,372). A atividade antioxidante, os polifenóis totais, o valor energético, o °Brix e a fibra aumentaram, respetivamente, 9,6, 6,1, 4,4, 4,3 e 3,4 vezes. As ameixas desidratadas apresentaram valores mais elevados de acidez e polifenóis totais (p≤0,05), as cerejas de água, glucose, frutose e aw (p≤0,05), as nectarinas de proteína, sacarose e fibra (p≤0,05) e os pêssegos de atividade antioxidante, gordura e hidratos de carbono (p≤0,05). A cereja foi o único fruto que apresentou teores médios de sódio acima do LQ (0,520 mg/100g). Conclui-se que a tecnologia utilizada apresenta enorme potencial para conservação (sem aditivos) e consequente valorização de prunóideas para comercialização fora de época.info:eu-repo/semantics/publishedVersio

Atmospheric effects on extensive air showers observed with the Surface Detector of the Pierre Auger Observatory

Atmospheric parameters, such as pressure (P), temperature (T) and density, affect the development of extensive air showers initiated by energetic cosmic rays. We have studied the impact of atmospheric variations on extensive air showers by means of the surface detector of the Pierre Auger Observatory. The rate of events shows a ~10% seasonal modulation and ~2% diurnal one. We find that the observed behaviour is explained by a model including the effects associated with the variations of pressure and density. The former affects the longitudinal development of air showers while the latter influences the Moliere radius and hence the lateral distribution of the shower particles. The model is validated with full simulations of extensive air showers using atmospheric profiles measured at the site of the Pierre Auger Observatory.Comment: 24 pages, 9 figures, accepted for publication in Astroparticle Physic

The exposure of the hybrid detector of the Pierre Auger Observatory

The Pierre Auger Observatory is a detector for ultra-high energy cosmic rays. It consists of a surface array to measure secondary particles at ground level and a fluorescence detector to measure the development of air showers in the atmosphere above the array. The "hybrid" detection mode combines the information from the two subsystems. We describe the determination of the hybrid exposure for events observed by the fluorescence telescopes in coincidence with at least one water-Cherenkov detector of the surface array. A detailed knowledge of the time dependence of the detection operations is crucial for an accurate evaluation of the exposure. We discuss the relevance of monitoring data collected during operations, such as the status of the fluorescence detector, background light and atmospheric conditions, that are used in both simulation and reconstruction.Comment: Paper accepted by Astroparticle Physic