Impacto de la variabilidad genotípica y ambiental sobre los carbohidratos solubles en el grano de soja

Optimizar la fecha de siembra del cultivo resulta crítico para maximizar el rendimiento (Di Mauro et al., 2018). Sin embargo, sus efectos sobre la calidad de los granos, más allá de la concentración de proteína y aceite, han sido raramente descriptos (Bosaz et al., 2019).A los efectos prácticos, el grano de soja está compuesto por: proteína, aceite y residual. El residual contiene cenizas, carbohidratos solubles (azúcares y oligosacáridos) e insolubles (celulosa y hemicelulosa) y lignina. Estos últimos son los componentes mayoritarios de la fibra dietaria (Westgate, 1999). La proteína y el aceite son los componentes de mayor valor económico y nutricional, representando en conjunto el 60% del peso del grano expresado en base seca, las cenizas corresponden a un 5% aproximadamente, mientras que un 35% pertenece a carbohidratos. Estos últimos se encuentran principalmente en la cubierta, pero también se pueden encontrar en las células del parénquima del embrión. Una porción de carbohidratos y lignina se elimina con las cascarillas, pero la harina de soja puede todavía contener hasta un 40% de carbohidratos totales (Medic et al., 2014). El residual es la fracción menos estudiada del grano de soja por ser el componente de menor retribución monetaria y calidad nutricional (Middelbos y Fahey, 2008). Sin embargo, su concentración y composición puede afectar el rendimiento y calidad de los ingredientes proteicos derivados. El residual, contiene rafinosa y estaquiosa, dos galactooligosacáridos de tres y cuatro monómeros respectivamente, con efectos antinutricionales. Estos oligosacáridos producen una disminución en la absorción intestinal de nutrientes, flatulencias y diarrea en cerdos, perros y humanos (Kumar et al., 2010). Genotipos con baja concentración de estaquiosa y elevada concentración de sacarosa han sido desarrollados para evitar dichas características. Su utilización en la elaboración de ingredientes proteicos ha conducido a una disminución en la concentración de estaquiosa en los mismos, aumentando la eficiencia del proceso y generando propiedades funcionales únicas (Deak y Johnson, 2006).El objetivo de este estudio consistió en describir cómo los genotipos (G) y el ambiente (A) afectan a la proteína, el aceite y los carbohidratos solubles (estaquiosa, rafinosa, glucosa y fructosa) dentro de la fracción residual en el grano de soja.Fil: Lopez, E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Alvarez Prado, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Rotundo, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Corteva Agriscience; Estados UnidosFil: Gerde, Jose Arnaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentin

Proinflammatory cytokine profile differences between primary open angle and pseudoexfoliative glaucoma

Introduction: Few studies have investigated glaucoma biomarkers in aqueous humor and tear and have found elevations of proinflammatory cytokines in patients with primary open-angle glaucoma (POAG) and pseudoexfoliative glaucoma (PXG). In this study we investigate differences in inflammatory cytokines between POAG and PXG patients to find specific disease biomarkers. Methods: For this purpose, tear and aqueous humor samples of 14 eyes with POAG and 15 eyes with PXG undergoing cataract surgery were immunoassayed for 27 pro-inflammatory cytokines. The concentrations of cytokines in tear and aqueous humor and their association with clinical variables were analysed, correlated and compared between the groups. Results: We found that the levels of three cytokines differed significantly in the aqueous humor of POAG and PXG patients: IL-12 and IL-13 were higher in the POAG group, while MCP-1(MCAF) was higher in the PXG group. The number of topical hypotensive medications was correlated with diminished levels of two cytokines (IL-7 and basic fibroblast growth factor) in aqueous humor in the POAG group and with diminished levels of IL-12 in tear in the PXG group. Conclusion: We conclude that both POAG and PXG show elevated concentrations of proinflammatory cytokines in tear and aqueous humor that could be used as biomarkers for these types of glaucoma and that the concentrations in aqueous humor of three cytokines: IL-12, IL-13 and MCP-1(MCAF) could be used to differentiate POAG and PXG

Evaluación de la calidad global de la colección de guías prácticas de heridas del servicio gallego de salud

Objetivo: Evaluar el nivel de calidad global de la colección de guías prácticas de heridas del Servicio Gallego de Salud (Sergas) para la toma de decisiones clínicas. Método: Evaluación de guías basadas en la evidencia (GBP). Selección de todas las GBP que componen la colección sobre heridas publicadas por el Sergas. Valoración a través del instrumento AGREE II-GRS (Global Rating Scale). Revisión por tres experto/as. Resultados: Fueron evaluadas siete GBP: úlceras por presión, úlceras de la extremidad inferior, pie diabético, lesiones cutáneas neoplásicas, lesiones por quemadura, herida quirúrgica aguda y lesiones cutáneas asociadas a la humedad. Se evaluaron cuatro apartados: el proceso de desarrollo, el estilo de presentación, la integridad de la información y la validez clínica. Todas las GBP obtuvieron una puntuación entre 5 y 7. A nivel global se obtuvo una nota media de 6 (“calidad alta”). Conclusiones: Las GBP publicadas por el Sergas son guías de muy buena calidad, recomendables para el uso clínico y aconsejable su implementación en los procesos de toma de decisiones en el área de las úlceras y heridas.Objective: To appraise the quality global level of the collection of practical guides for wounds of the Galician Health Service (Sergas) for professional decision-making. Method: Evaluation of Best Practice Guidelines (BPG). Selection of all BPG of the collection on injuries/wounds published by Sergas. Valuation through the AGREE II-GRS instrument (Global Rating Scale). Review by three experts. Results: Seven BPG were evaluated: pressure ulcers, ulcers of the lower limb, diabetic foot, skin tumor lesions, burn injuries, acute surgical wound and skin lesions associated with humidity. Four sections were evaluated: the development process, the presentation style, the integrity of the information and the clinical validity. All the GBP obtained a score between 5 and 7. On a global level, an average score of 6 was obtained (High quality). Conclusions: The BPG published by Sergas are guides of very good quality, recommended for clinical use and advisable to implement them in decision-making processes in the area of ulcers and wounds

Antitubercular drugs for an old target: GSK693 as a promising InhA direct inhibitor

AbstractDespite being one of the first antitubercular agents identified, isoniazid (INH) is still the most prescribed drug for prophylaxis and tuberculosis (TB) treatment and, together with rifampicin, the pillars of current chemotherapy. A high percentage of isoniazid resistance is linked to mutations in the pro-drug activating enzyme KatG, so the discovery of direct inhibitors (DI) of the enoyl-ACP reductase (InhA) has been pursued by many groups leading to the identification of different enzyme inhibitors, active against Mycobacterium tuberculosis (Mtb), but with poor physicochemical properties to be considered as preclinical candidates. Here, we present a series of InhA DI active against multidrug (MDR) and extensively (XDR) drug-resistant clinical isolates as well as in TB murine models when orally dosed that can be a promising foundation for a future treatment

Perfil clínico de los pacientes tratados con evolocumab en unidades hospitalarias de nefrología en España

Describir las características clínicas de los pacientes tratados con evolocumab, las razones del inicio de la terapia y los efectos del tratamiento en la fase inicial de disponibilidad de evolocumab en las unidades de nefrología de España

Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication

International audienceno abstrac

Effectiveness of a cognitive behavioral intervention in patients with medically unexplained symptoms: cluster randomized trial

BACKGROUND: Medically unexplained symptoms are an important mental health problem in primary care and generate a high cost in health services.Cognitive behavioral therapy and psychodynamic therapy have proven effective in these patients. However, there are few studies on the effectiveness of psychosocial interventions by primary health care. The project aims to determine whether a cognitive-behavioral group intervention in patients with medically unexplained symptoms, is more effective than routine clinical practice to improve the quality of life measured by the SF-12 questionary at 12 month. METHODS/DESIGN: This study involves a community based cluster randomized trial in primary healthcare centres in Madrid (Spain). The number of patients required is 242 (121 in each arm), all between 18 and 65 of age with medically unexplained symptoms that had seeked medical attention in primary care at least 10 times during the previous year. The main outcome variable is the quality of life measured by the SF-12 questionnaire on Mental Healthcare. Secondary outcome variables include number of consultations, number of drug (prescriptions) and number of days of sick leave together with other prognosis and descriptive variables. Main effectiveness will be analyzed by comparing the percentage of patients that improve at least 4 points on the SF-12 questionnaire between intervention and control groups at 12 months. All statistical tests will be performed with intention to treat. Logistic regression with random effects will be used to adjust for prognostic factors. Confounding factors or factors that might alter the effect recorded will be taken into account in this analysis. DISCUSSION: This study aims to provide more insight to address medically unexplained symptoms, highly prevalent in primary care, from a quantitative methodology. It involves intervention group conducted by previously trained nursing staff to diminish the progression to the chronicity of the symptoms, improve quality of life, and reduce frequency of medical consultations. TRIAL REGISTRATION: The trial was registered with ClinicalTrials.gov, number NCT01484223 [http://ClinicalTrials.gov].S

Regulatory sites for splicing in human basal ganglia are enriched for disease-relevant information

Genome-wide association studies have generated an increasing number of common genetic variants associated with neurological and psychiatric disease risk. An improved understanding of the genetic control of gene expression in human brain is vital considering this is the likely modus operandum for many causal variants. However, human brain sampling complexities limit the explanatory power of brain-related expression quantitative trait loci (eQTL) and allele-specific expression (ASE) signals. We address this, using paired genomic and transcriptomic data from putamen and substantia nigra from 117 human brains, interrogating regulation at different RNA processing stages and uncovering novel transcripts. We identify disease-relevant regulatory loci, find that splicing eQTLs are enriched for regulatory information of neuron-specific genes, that ASEs provide cell-specific regulatory information with evidence for cellular specificity, and that incomplete annotation of the brain transcriptome limits interpretation of risk loci for neuropsychiatric disease. This resource of regulatory data is accessible through our web server, http://braineacv2.inf.um.es/

Identification of novel risk loci, causal insights, and heritable risk for Parkinson's disease: a meta-analysis of genome-wide association studies

Background Genome-wide association studies (GWAS) in Parkinson's disease have increased the scope of biological knowledge about the disease over the past decade. We aimed to use the largest aggregate of GWAS data to identify novel risk loci and gain further insight into the causes of Parkinson's disease. Methods We did a meta-analysis of 17 datasets from Parkinson's disease GWAS available from European ancestry samples to nominate novel loci for disease risk. These datasets incorporated all available data. We then used these data to estimate heritable risk and develop predictive models of this heritability. We also used large gene expression and methylation resources to examine possible functional consequences as well as tissue, cell type, and biological pathway enrichments for the identified risk factors. Additionally, we examined shared genetic risk between Parkinson's disease and other phenotypes of interest via genetic correlations followed by Mendelian randomisation. Findings Between Oct 1, 2017, and Aug 9, 2018, we analysed 7·8 million single nucleotide polymorphisms in 37 688 cases, 18 618 UK Biobank proxy-cases (ie, individuals who do not have Parkinson's disease but have a first degree relative that does), and 1·4 million controls. We identified 90 independent genome-wide significant risk signals across 78 genomic regions, including 38 novel independent risk signals in 37 loci. These 90 variants explained 16–36% of the heritable risk of Parkinson's disease depending on prevalence. Integrating methylation and expression data within a Mendelian randomisation framework identified putatively associated genes at 70 risk signals underlying GWAS loci for follow-up functional studies. Tissue-specific expression enrichment analyses suggested Parkinson's disease loci were heavily brain-enriched, with specific neuronal cell types being implicated from single cell data. We found significant genetic correlations with brain volumes (false discovery rate-adjusted p=0·0035 for intracranial volume, p=0·024 for putamen volume), smoking status (p=0·024), and educational attainment (p=0·038). Mendelian randomisation between cognitive performance and Parkinson's disease risk showed a robust association (p=8·00 × 10−7). Interpretation These data provide the most comprehensive survey of genetic risk within Parkinson's disease to date, to the best of our knowledge, by revealing many additional Parkinson's disease risk loci, providing a biological context for these risk factors, and showing that a considerable genetic component of this disease remains unidentified. These associations derived from European ancestry datasets will need to be followed-up with more diverse data. Funding The National Institute on Aging at the National Institutes of Health (USA), The Michael J Fox Foundation, and The Parkinson's Foundation (see appendix for full list of funding sources)

Moving beyond neurons: the role of cell type-specific gene regulation in Parkinson's disease heritability

Parkinson’s disease (PD), with its characteristic loss of nigrostriatal dopaminergic neurons and deposition of α-synuclein in neurons, is often considered a neuronal disorder. However, in recent years substantial evidence has emerged to implicate glial cell types, such as astrocytes and microglia. In this study, we used stratified LD score regression and expression-weighted cell-type enrichment together with several brain-related and cell-type-specific genomic annotations to connect human genomic PD findings to specific brain cell types. We found that PD heritability attributable to common variation does not enrich in global and regional brain annotations or brain-related cell-type-specific annotations. Likewise, we found no enrichment of PD susceptibility genes in brain-related cell types. In contrast, we demonstrated a significant enrichment of PD heritability in a curated lysosomal gene set highly expressed in astrocytic, microglial, and oligodendrocyte subtypes, and in LoF-intolerant genes, which were found highly expressed in almost all tested cellular subtypes. Our results suggest that PD risk loci do not lie in specific cell types or individual brain regions, but rather in global cellular processes detectable across several cell types