Produção de inóculo de Trichoderma viride para o controle da armilariose em Pinus spp.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Characterization of \u3ci\u3eNeofabraea actinidiae\u3c/i\u3e and \u3ci\u3eN. brasiliensis\u3c/i\u3e as causal agents of apple bull’s-eye rot in southern Brazil

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in production of enzymes in the two species, with all N. brasiliensis isolates showing no production of pectolyase at pH 7. The lowest EC50 and EC100 values were observed with thiophanate methyl. In general, ‘Fuji’ fruits were more susceptible to Neofabraea infection and had larger lesions, while N. brasiliensis isolates showed greater aggressiveness on ‘Fuji’ hybrid and ‘Pink lady’ fruits compared with N. actinidiae. Dans le sud du Brésil, les agents causaux du chancre gloésporien de la pomme ont récemment été décrits comme étant Neofabraea actinidiae et N. brasiliensis. Des isolats des deux espèces ont été évalués en fonction de la réaction de l’indice de croissance mycélienne à différentes températures, de la production enzymatique, de l’inhibition de la croissance mycélienne et des concentrations efficaces (CE50 et CE100) des fongicides triflumizole, pyriméthanil et triophanate méthyle, ainsi qu’en fonction de leur agressivité à l’égard de la pomme hybride Fuji et de la Pink Lady. La croissance mycélienne chez N. brasiliensis était considérablement plus faible que chez N. actinidiae, et ce, à toutes les températures testées. Aucune espèce ne s’est développée à 3 ou à 32°C. Chez les deux espèces, il y a eu de petites différences quant à la production enzymatique, tous les isolats de N. brasiliensis n’affichant aucune production de pectolyase à pH 7. Les plus faibles valeurs de CE50 et de CE100 observées ont été celles du triophanate méthyle. Dans l’ensemble, les Fuji étaient plus sujettes à l’infection causée par Neofabraea et affichaient des lésions plus étendues, tandis que les isolats de N. brasiliensis étaient plus agressifs à l’égard de l’hybride Fuji et de la Pink Lady que ceux de N. actinidiae

Characterization of \u3ci\u3eNeofabraea actinidiae\u3c/i\u3e and \u3ci\u3eN. brasiliensis\u3c/i\u3e as causal agents of apple bull’s-eye rot in southern Brazil

The causal agents of apple bull’s-eye rot in southern Brazil have recently been described as Neofabraea actinidiae and N. brasiliensis. Isolates of both species were evaluated for response of mycelial growth index (MGI) to different temperatures, enzyme production, mycelial growth inhibition and effective concentrations (EC50 and EC100) of the fungicides triflumizole, pyrimethanil and thiophanate methyl, as well as aggressiveness on fruits of ‘Fuji’ hybrid and ‘Pink Lady’. There was significantly lower mycelium growth in N. brasiliensis compared with N. actinidiae at all temperatures tested. Neither species grew at 3 and 32°C. There were minor differences in production of enzymes in the two species, with all N. brasiliensis isolates showing no production of pectolyase at pH 7. The lowest EC50 and EC100 values were observed with thiophanate methyl. In general, ‘Fuji’ fruits were more susceptible to Neofabraea infection and had larger lesions, while N. brasiliensis isolates showed greater aggressiveness on ‘Fuji’ hybrid and ‘Pink lady’ fruits compared with N. actinidiae. Dans le sud du Brésil, les agents causaux du chancre gloésporien de la pomme ont récemment été décrits comme étant Neofabraea actinidiae et N. brasiliensis. Des isolats des deux espèces ont été évalués en fonction de la réaction de l’indice de croissance mycélienne à différentes températures, de la production enzymatique, de l’inhibition de la croissance mycélienne et des concentrations efficaces (CE50 et CE100) des fongicides triflumizole, pyriméthanil et triophanate méthyle, ainsi qu’en fonction de leur agressivité à l’égard de la pomme hybride Fuji et de la Pink Lady. La croissance mycélienne chez N. brasiliensis était considérablement plus faible que chez N. actinidiae, et ce, à toutes les températures testées. Aucune espèce ne s’est développée à 3 ou à 32°C. Chez les deux espèces, il y a eu de petites différences quant à la production enzymatique, tous les isolats de N. brasiliensis n’affichant aucune production de pectolyase à pH 7. Les plus faibles valeurs de CE50 et de CE100 observées ont été celles du triophanate méthyle. Dans l’ensemble, les Fuji étaient plus sujettes à l’infection causée par Neofabraea et affichaient des lésions plus étendues, tandis que les isolats de N. brasiliensis étaient plus agressifs à l’égard de l’hybride Fuji et de la Pink Lady que ceux de N. actinidiae

First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America

The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes, which in turn are underpinned by land-use modifications that alter the governance of pathogen, biodiversity and human interactions. Our current understanding of these ecological changes on disease emergence however remains limited. Buruli ulcer is an emerging human skin disease caused by the mycobacterium, Mycobacterium ulcerans, for which the exact route of infection remains unclear. It can have a devastating impact on its human host, causing extensive necrosis of the skin and underlying tissue, often leading to permanent disability. The mycobacterium is associated with tropical aquatic environments and incidences of the disease are significantly higher on floodplains and where there is an increase of human aquatic activities. Although the disease has been previously diagnosed in South America, until now the presence of M. ulcerans DNA in the wild has only been identified in Australia where there have been significant outbreaks and in western and central regions of Africa where the disease is persistent. Here for the first time, we have identified the presence of the aetiological agent's DNA in environmental samples from South America. The DNA was positively identified using Real-time Polymerase Chain Reaction (PCR) on 163 environmental samples, taken from 23 freshwater bodies in French Guiana (Southern America), using primers for both IS2404 and for the ketoreductase-B domain of the M. ulcerans mycolactone polyketide synthase genes (KR). Five samples out of 163 were positive for both primers from three different water bodies. A further nine sites had low levels of IS2404 close to a standard CT of 35 and could potentially harbour M. ulcerans. The majority of our positive samples (8/14) came from filtered water. These results also reveal the Sinnamary River as a potential source of infection to humans. © 2014 Morris et al

The role of ongoing dendritic oscillations in single-neuron dynamics

The dendritic tree contributes significantly to the elementary computations a neuron performs while converting its synaptic inputs into action potential output. Traditionally, these computations have been characterized as temporally local, near-instantaneous mappings from the current input of the cell to its current output, brought about by somatic summation of dendritic contributions that are generated in spatially localized functional compartments. However, recent evidence about the presence of oscillations in dendrites suggests a qualitatively different mode of operation: the instantaneous phase of such oscillations can depend on a long history of inputs, and under appropriate conditions, even dendritic oscillators that are remote may interact through synchronization. Here, we develop a mathematical framework to analyze the interactions of local dendritic oscillations, and the way these interactions influence single cell computations. Combining weakly coupled oscillator methods with cable theoretic arguments, we derive phase-locking states for multiple oscillating dendritic compartments. We characterize how the phase-locking properties depend on key parameters of the oscillating dendrite: the electrotonic properties of the (active) dendritic segment, and the intrinsic properties of the dendritic oscillators. As a direct consequence, we show how input to the dendrites can modulate phase-locking behavior and hence global dendritic coherence. In turn, dendritic coherence is able to gate the integration and propagation of synaptic signals to the soma, ultimately leading to an effective control of somatic spike generation. Our results suggest that dendritic oscillations enable the dendritic tree to operate on more global temporal and spatial scales than previously thought

First Results from High Angular Resolution ALMA Observations Toward the HL Tau Region

We present Atacama Large Millimeter/submillimeter Array (ALMA) observations from the 2014 Long Baseline Campaign in dust continuum and spectral line emission from the HL Tau region. The continuum images at wavelengths of 2.9, 1.3, and 0.87 mm have unprecedented angular resolutions of 0.075 arcseconds (10 AU) to 0.025 arcseconds (3.5 AU), revealing an astonishing level of detail in the circumstellar disk surrounding the young solar analogue HL Tau, with a pattern of bright and dark rings observed at all wavelengths. By fitting ellipses to the most distinct rings, we measure precise values for the disk inclination (46.72pm0.05 degrees) and position angle (+138.02pm0.07 degrees). We obtain a high-fidelity image of the 1.0 mm spectral index ($\alpha$), which ranges from $\alpha\sim2.0$ in the optically-thick central peak and two brightest rings, increasing to 2.3-3.0 in the dark rings. The dark rings are not devoid of emission, we estimate a grain emissivity index of 0.8 for the innermost dark ring and lower for subsequent dark rings, consistent with some degree of grain growth and evolution. Additional clues that the rings arise from planet formation include an increase in their central offsets with radius and the presence of numerous orbital resonances. At a resolution of 35 AU, we resolve the molecular component of the disk in HCO+ (1-0) which exhibits a pattern over LSR velocities from 2-12 km/s consistent with Keplerian motion around a ~1.3 solar mass star, although complicated by absorption at low blue-shifted velocities. We also serendipitously detect and resolve the nearby protostars XZ Tau (A/B) and LkHa358 at 2.9 mm.Comment: 11 pages, 5 figures, 2 tables, accepted for publication in the Astrophysical Journal Letter

Molecular Line Emission Towards High-Mass Clumps: The MALT90 Catalogue

The Millimetre Astronomy Legacy Team 90 GHz (MALT90) survey aims to characterise the physical and chemical evolution of high-mass clumps. Recently completed, it mapped 90 GHz line emission toward 3246 high-mass clumps identified from the ATLASGAL 870 �m Galactic plane survey. By utilising the broad frequency coverage of the Mopra telescope's spectrometer, maps in 16 different emission lines were simultaneously obtained. Here we describe the �first line catalog of the detected emission, generated by Gaussian profile �fitting to spectra extracted toward each clumps' dust peak. Synthetic spectra show that the catalog has a completeness of >95%, a probability of a false-positive detection of <0.3%, and a relative uncertainty in the measured quantities of <20% over the range of detection criteria. We find that the detection rates are highest for the (1{0) molecular transitions of HCO+, HNC, N2H+, and HCN (72{88%). The majority of clumps (~� 95%) are detected in at least one of the molecular transitions, just under half of the clumps (�~48%) are detected in 4 or more of the transitions, while only 2 clumps are detected in 13 or more transitions. We find several striking trends in the ensemble of properties for the different molecular transitions when plotted as a function of the clumps' evolutionary state. In particular, the optically thickest HCO+ emission shows a `blue-red asymmetry' that indicates overall collapse that monotonically decreases as the clumps evolve. This catalog represents the largest compiled database of molecular line emission toward high-mass clumps and is a valuable data set for detailed studies of these objects

Effect of Feedback of Massive Stars in the Fragmentation, Distribution, and Kinematics of the Gas in Two Star-forming Regions in the Carina Nebula

We present ALMA high spatial resolution observations toward two star-forming regions located in one of the most extreme zones of star formation in the Galaxy, the Carina Nebula. One region is located at the center of the nebula and is severally affected by the stellar feedback from high-mass stars, while the other region is located farther south and is less disturbed by the massive-star clusters. We found that the region at the center of the nebula is forming less but more massive cores than the region located in the south, suggesting that the level of stellar feedback effectively influences the fragmentation process in clumps. Lines such as HCN, HCO+, and SiO show abundant and complex gas distributions in both regions, confirming the presence of ionization and shock fronts. Jeans analysis suggests that the observed core masses in the region less affected by the massive stars are consistent with thermal fragmentation, but turbulent Jeans fragmentation might explain the high masses of the cores identified in the region in the center of Carina. Consistently, two different analyses in the HCO+ line provided evidence for a higher level of turbulence in the gas more affected by the stellar feedback. The gas column density probability functions, N-pdf’s, show lognormal shapes with clear transitions to power-law regimes. We observed a wider N-pdf in the region at the center of the nebula, which provides further evidence for a higher level of turbulence in the material with a higher level of massive stellar feedback.Indexación: Scopu

MALT90 Kinematic Distances to Dense Molecular Clumps

Using molecular-line data from the Millimetre Astronomy Legacy Team 90 GHz Survey (MALT90), we have estimated kinematic distances to 1905 molecular clumps identified in the ATLASGAL 870 μm continuum survey over the longitude range 295° < l < 350°. The clump velocities were determined using a flux-weighted average of the velocities obtained from Gaussian fits to the HCO+, HNC, and N2H+ (1–0) transitions. The near/far kinematic distance ambiguity was addressed by searching for the presence or absence of absorption or self-absorption features in 21 cm atomic hydrogen spectra from the Southern Galactic Plane Survey. Our algorithm provides an estimation of the reliability of the ambiguity resolution. The Galactic distribution of the clumps indicates positions where the clumps are bunched together, and these locations probably trace the locations of spiral arms. Several clumps fall at the predicted location of the far side of the Scutum–Centaurus arm. Moreover, a number of clumps with positive radial velocities are unambiguously located on the far side of the Milky Way at galactocentric radii beyond the solar circle. The measurement of these kinematic distances, in combination with continuum or molecular-line data, now enables the determination of fundamental parameters such as mass, size, and luminosity for each clump

High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons

The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.National Institutes of Health (U.S.) (MH095096)National Institutes of Health (U.S.) (R01 GM089652