Eine juedisch-evangelische Liebe in schwerer Zeit : die Geschichte meiner Eltern 1939-1949.

Anneliese Seidel’s father, who was Jewish, escaped Nazi Germany and emigrated to Guatemala in 1939. Anneliese, her brother Horst, and their Christian Protestant mother remained in Breslau, moving after WW II to East- and then to West-Germany. In 1949 they joined their father/husband in Guatemala.digitizedEpilogue by Martin Richau, BerlinAnneliese Heilborn Seidel was born 1934 in Breslau, the daughter of Heimann/Heinz and Margarethe, née Niedergesaess

Selective Conjugation of Proteins by Mining Active Proteomes through Click-Functionalized Magnetic Nanoparticles

Superparamagnetic iron oxide nanoparticles (SPIONs) coated with azide groups were functionalized at the surface with biotin (biotin@SPIONs) and cysteine protease inhibitor E-64 (E-64@SPION5) with the purpose of developing nanoparticle-based assays for identifying cysteine proteases in proteomes. Magnetite particles (ca. 6 nm) were synthesized by microwave-assisted thermal decomposition of iron acetylacetonate and subsequently functionalized following a click chemistry protocol to obtain biotin and E-64 labeled particulate systems. Successful surface modification and covalent attachment of functional groups and molecules were confirmed by FT-IR spectroscopy and thermal gravimetric analysis. The ability of the surface-grafted biotin terminal groups to specifically interact with streptavidin (either horseradish peroxidase [(HRP)-luminol-H2O2] or rhodamine) was confirmed by chemiluminescent assay. A quantitative assessment showed a capture limit of 0.55-1.65 mu g protein/100 mu g particles. Furthermore, E-64@SPION5 were successfully used to specifically label papain-like cysteine proteases from crude plant extracts. Owing to the simplicity and versatility of the technique, together with the superparamagnetic behavior of FeOx-nanoparticles, the results demonstrate that click chemistry on surface anchored azide group is a viable approach toward bioconjugations that can be extended to other nanoparticles surfaces with different functional groups to target specific therapeutic and diagnostic applications