562 research outputs found
SUSTAINABLE DESIGN EVALUATION – INTEGRATION OF SUSTAINABILITY IN PRODUCT DEVELOPMENT PROCESSES
To develop sustainable products, product developers must be able to incorporate sustainability into individual decision processes during product development. In doing so, they are faced with the conflicting demands of time and cost pressure and the growing complexity caused by the many requirements and (sustainability) criteria.
The Sustainable Design Evaluation, which is presented in this publication, is a method to enable product developers to estimate the impact of their product-related decisions along the three dimensions of sustainability across all phases of the product life cycle. The core of the Sustainable Design Evaluation is a two-stage assessment technique enabling a relative and comparable quantification of ecological, economic, and social criteria. Furthermore, an aggregation scheme for those criteria is introduced. Based on the two-stage assessment technique and the system of aggregation, the results of the Sustainable Design Evaluation can be displayed clearly and interpreted easily by product developers to assess the impacts of their product-related decisions.
Thus, in contrast to existing methods, the SDE combines ease of use and interpretation with a sufficiently holistic sustainability assessment
Cognitive ability is heritable and predicts the success of an alternative mating tactic
The research was funded through the Czech Science Foundation 457 (P505/12/G112).The ability to attract mates, acquire resources for reproduction, and successfully outcompete rivals for fertilisations may make demands on cognitive traits - the mechanisms by which an animal acquires, processes, stores, and acts upon information from its environment. Consequently, cognitive traits potentially undergo sexual selection in some mating systems. We investigated the role of cognitive traits on the reproductive performance of male rose bitterling (Rhodeus ocellatus), a freshwater fish with a complex mating system and alternative mating tactics. We quantified the learning accuracy of males and females in a spatial learning task and scored them for learning accuracy. Males were subsequently allowed to play the roles of a guarder and a sneaker in competitive mating trials, with reproductive success measured using paternity analysis. We detected a significant interaction between male mating role and learning accuracy on reproductive success, with the best performing males in maze trials showing greater reproductive success in a sneaker role than as a guarder. Using a cross-classified breeding design, learning accuracy was demonstrated to be heritable, with significant additive maternal and paternal effects. Our results imply that male cognitive traits may undergo intra-sexual selection.PostprintPeer reviewe
Euglena gracilis Ribonucleotide Reductase THE EUKARYOTE CLASS II ENZYME AND THE POSSIBLE ANTIQUITY OF EUKARYOTE B12 DEPENDENCE
Ribonucleotide reductases provide the building blocks for DNA synthesis. Three classes of enzymes are known, differing widely in amino acid sequence but with similar structural motives and allosteric regulation. Class I occurs in eukaryotes and aerobic prokaryotes, class II occurs in aerobic and anaerobic prokaryotes, and class III occurs in anaerobic prokaryotes. The eukaryote Euglena gracilis contains a class II enzyme (Gleason, F. K., and Hogenkamp, H. P. (1970) J. Biol. Chem. 245, 4894-4899) and, thus, forms an exception. Class II enzymes depend on vitamin B(12) for their activity. We purified the reductase from Euglena cells, determined partial peptide sequences, identified its cDNA, and purified the recombinant enzyme. Its amino acid sequence and general properties, including its allosteric behavior, were similar to the class II reductase from Lactobacillus leichmannii. Both enzymes belong to a distinct small group of reductases that unlike all other homodimeric reductases are monomeric. They compensate the loss of the second polypeptide of dimeric enzymes by a large insertion in the monomeric chain. Data base searching and sequence comparison revealed a homolog from the eukaryote Dictyostelium discoideum as the closest relative to the Euglena reductase, suggesting that the class II enzyme was present in a common, B(12)-dependent, eukaryote ancestor
2000 Philip C. Jessup International Moot Court Competition
Merapi and Erebus have agreed to submit the Case Concerning The Seabed Mining Facility to the ICJ pursuant to Art.36(1) of the Statute of the ICJ
Patterns and drivers of Nothobranchius killifish diversity in lowland Tanzania.
Temporary pools are seasonal wetland habitats with specifically adapted biota, including annual Nothobranchius killifishes that survive habitat desiccation as diapausing eggs encased in dry sediment. To understand the patterns in the structure of Nothobranchius assemblages and their potential in wetland conservation, we compared biodiversity components (alpha, beta, and gamma) between regions and estimated the role and sources of nestedness and turnover on their diversity. We sampled Nothobranchius assemblages from 127 pools across seven local regions in lowland Eastern Tanzania over 2 years, using dip net and seine nets. We estimated species composition and richness for each pool, and beta and gamma diversity for each region. We decomposed beta diversity into nestedness and turnover components. We tested nestedness in three main regions (Ruvu, Rufiji, and Mbezi) using the number of decreasing fills metric and compared the roles of pool area, isolation, and altitude on nestedness. A total of 15 species formed assemblages containing 1-6 species. Most Nothobranchius species were endemic to one or two adjacent regions. Regional diversity was highest in the Ruvu, Rufiji, and Mbezi regions. Nestedness was significant in Ruvu and Rufiji, with shared core (N. melanospilus, N. eggersi, and N. janpapi) and common (N. ocellatus and N. annectens) species, and distinctive rare species. Nestedness apparently resulted from selective colonization rather than selective extinction, and local species richness was negatively associated with altitude. The Nothobranchius assemblages in the Mbezi region were not nested, and had many endemic species and the highest beta diversity driven by species turnover. Overall, we found unexpected local variation in the sources of beta diversity (nestedness and turnover) within the study area. The Mbezi region contained the highest diversity and many endemic species, apparently due to repeated colonizations of the region rather than local diversification. We suggest that annual killifish can serve as a flagship taxon for small wetland conservation
Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor: Honokiol and Muscarinic Signaling
Background: Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees and present in Asian herbal teas, has a unique array of pharmacological actions, including the inhibition of multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol; cytotoxicity was determined using a dye reduction assay.
Results: Honokiol had a potent (EC50 ≈ 5 μmol/l) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and partially reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding.
Conclusions: It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties
Oxidative Stress Disruption of Receptor-Mediated Calcium Signaling Mechanisms
Background: Oxidative stress increases the cytosolic content of calcium in the cytoplasm through a combination of effects on calcium pumps, exchangers, channels and binding proteins. In this study, oxidative stress was produced by exposure to tert-butyl hydroperoxide (tBHP); cell viability was assessed using a dye reduction assay; receptor binding was characterized using [3H]N-methylscopolamine ([3H]MS); and cytosolic and luminal endoplasmic reticulum (ER) calcium concentrations ([Ca2+]i and [Ca2+]L, respectively) were measured by fluorescent imaging.
Results: Activation of M3 muscarinic receptors induced a biphasic increase in [Ca2+]i: an initial, inositol trisphosphate (IP3)-mediated release of Ca2+ from endoplasmic reticulum (ER) stores followed by a sustained phase of Ca2+ entry (i.e., store-operated calcium entry; SOCE). Under non-cytotoxic conditions, tBHP increased resting [Ca2+]i; a 90 minute exposure to tBHP (0.5-10 mM) increased [Ca2+]i from 26 to up to 127 nM and decreased [Ca2+]L by 55%. The initial response to 10 μM carbamylcholine was depressed by tBHP in the absence, but not the presence, of extracellular calcium. SOCE, however, was depressed in both the presence and absence of extracellular calcium. Acute exposure to tBHP did not block calcium influx through open SOCE channels. Activation of SOCE following thapsigargin-induced depletion of ER calcium was depressed by tBHP exposure. In calcium-free media, tBHP depressed both SOCE and the extent of thapsigargin-induced release of Ca2+ from the ER. M3 receptor binding parameters (ligand affinity, guanine nucleotide sensitivity, allosteric modulation) were not affected by exposure to tBHP.
Conclusions: Oxidative stress induced by tBHP affected several aspects of M3 receptor signaling pathway in CHO cells, including resting [Ca2+]i, [Ca2+]L, IP3 receptor mediated release of calcium from the ER, and calcium entry through the SOCE. tBHP had little effect on M3 receptor binding or G protein coupling. Thus, oxidative stress affects multiple aspects of calcium homeostasis and calcium dependent signaling
The GALEX Arecibo SDSS survey: III. Evidence for the Inside-Out Formation of Galactic Disks
We analyze a sample of galaxies with stellar masses greater than and with redshifts in the range for which HI mass
measurements are available from the GALEX Arecibo SDSS Survey (GASS) or from
the Arecibo Legacy Fast ALFA survey (ALFALFA). At a given value of , our
sample consists primarily of galaxies that are more HI-rich than average. We
constructed a series of three control samples for comparison with these HI-rich
galaxies. As expected, HI-rich galaxies differ strongly from galaxies of same
stellar mass that are selected without regard to HI content. The majority of
these differences are attributable to the fact that galaxies with more gas are
bluer and more actively star-forming. In order to identify those galaxy
properties that are causally connected with HI content, we compare results
derived for the HI sample with those derived for galaxies matched in stellar
mass, size and NUV- colour. The only photometric property that is clearly
attributable to increasing HI content, is the colour gradient of the galaxy.
Galaxies with larger HI fractions have bluer, more actively star-forming outer
disks compared to the inner part of the galaxy. HI-rich galaxies also have
larger -band radii compared to -band radii. Our results are consistent
with the "inside-out" picture of disk galaxy formation, which has commonly
served as a basis for semi-analytic models of the formation of disks in the
context of Cold Dark Matter cosmologies. The lack of any intrinsic connection
between HI fraction and galaxy asymmetry suggests that gas is accreted smoothly
onto the outer disk.Comment: 18 pages, 20 figures. Accepted for publication in MNRAS. GASS
publications and released data can be found at
http://www.mpa-garching.mpg.de/GASS/index.ph
Study of a homogeneous QSO sample: relations between the QSO and its host galaxy
We analyse a sample of 69 QSOs which have been randomly selected in a
complete sample of 104 QSOs (R<18, 0.142 < z < 0.198). 60 have been observed
with the NTT/SUSI2 at La Silla, through two filters in the optical band (WB#655
and V#812), and the remaining 9 are taken from archive databases. The filter
V#812 contains the redshifted Hbeta and forbidden [OIII] emission lines, while
WB#655 covers a spectral region devoid of emission lines, thus measuring the
QSO and stellar continua. The contributions of the QSO and the host are
separated thanks to the MCS deconvolution algorithm, allowing a morphological
classification of the host, and the computation of several parameters such as
the host and nucleus absolute V-magnitude, distance between the luminosity
center of the host and the QSO, and colour of the host and nucleus. We define a
new asymmetry coefficient, independent of any galaxy models and well suited for
QSO host studies. The main results from this study are: (i) 25% of the total
number of QSO hosts are spirals, 51% are ellipticals and 60% show signs of
interaction; (ii) Highly asymmetric systems tend to have a higher gas
ionization level (iii) Elliptical hosts contain a substantial amount of ionized
gas, and some show off-nuclear activity. These results agree with hierarchical
models merger driven evolution.Comment: accepted for publication in MNRAS, 19 pages, 22 figures, 8 table
Shining Light on Merging Galaxies I: The Ongoing Merger of a Quasar with a `Green Valley' Galaxy
Serendipitous observations of a pair z = 0.37 interacting galaxies (one
hosting a quasar) show a massive gaseous bridge of material connecting the two
objects. This bridge is photoionized by the quasar (QSO) revealing gas along
the entire projected 38 kpc sightline connecting the two galaxies. The emission
lines that result give an unprecedented opportunity to study the merger process
at this redshift. We determine the kinematics, ionization parameter (log U ~
-2.5 +- 0.03), column density (N_H ~ 10^{21} cm^{-2}), metallicity ([M/H] ~
-0.20 +- 0.15), and mass (~ 10^8 Msun) of the gaseous bridge. We simultaneously
constrain properties of the QSO-host (M_DM>8.8x 10^{11} Msun) and its companion
galaxy (M_DM>2.1 x 10^{11} Msun; M_star ~ 2 x 10^{10} Msun; stellar burst
age=300-800 Myr; SFR~6 Msun/yr; and metallicity 12+log (O/H)= 8.64 +- 0.2). The
general properties of this system match the standard paradigm of a
galaxy-galaxy merger caught between first and second passage while one of the
galaxies hosts an active quasar. The companion galaxy lies in the so-called
`green valley', with a stellar population consistent with a recent starburst
triggered during the first passage of the merger and has no detectable AGN
activity. In addition to providing case-studies of quasars associated with
galaxy mergers, quasar/galaxy pairs with QSO-photoionized tidal bridges such as
this one offer unique insights into the galaxy properties while also
distinguishing an important and inadequately understood phase of galaxy
evolution.Comment: 23 pages, 12 figures, 5 tables, Submitted to ApJ, revised to address
referee's comment
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