Relações entre características fenotípicas e de qualidade em acessos de linhaça nativos e exóticos

The objective of this work was to evaluate the relationship among phenotypic and quality traits in a set of indigenous and exotic accessions of linseed (Linum usitatissimum). The experimental material consisted of 151 accessions of linseed belonging to landraces and cultivars collected from diverse agroecological zones. Five randomly chosen plants of each accession in each replicate were tagged, and data were recorded for the 12 following agronomic traits: days to flowering, days to maturity, plant weight, plant height, tillers per plant, secondary branches per plant, capsules per plant, seeds per capsule, 1,000 seed weight, harvest index, seed yield per plant, and oil content. Capsules per plant, harvest index, and plant weight played a direct and indirect major role on seed yield. High heritability, coupled with high genetic advance for plant weight, secondary branches per plant, capsules per plant, and seed yield per plant, suggests that selection based on these traits can be effective. Regarding per se performance, the following accessions can be exploited for commercial cultivation: Shweta (5.41 g), Gaurav (5.07 g), and EX-3-3 (4.77 g) for seed yield; and Shubhra (45.09%), Mukta (44.94%), Laxmi-27 (45.06%), and Shweta (44.25%) for oil content. The chemical profiling of fatty acids obtained in the present study can provide a platform for the selection of accessions for the genetic improvement of linseed.O objetivo deste trabalho foi avaliar a relação entre as características fenotípicas e de qualidade de um conjunto de acessos nativos e exóticos de linhaça (Linum usitatissimum). O material avaliado consistiu de 151 acessos de linhaça nativos ou de cultivares obtidas de diferentes zonas agroecológicas. Cinco plantas de cada acesso, selecionadas aletoriamente, foram identificadas, e foram registrados dados para as 12 seguintes características agronômicas: número de dias para o início do florescimento, número de dias para a maturação, peso da planta, altura da planta, número de perfilhos por planta, número de ramos secundários por planta, número de cápsulas por planta, número de sementes por cápsula, peso de mil grãos, índice de colheita, produção de sementes por planta e conteúdo de óleo. O número de cápsulas por planta, o índice de colheita e o peso da planta desempenharam papel importante tanto direta quanto indiretamente na produção de sementes. Altos valores de herdabilidade, associados a alto avanço genético para peso da planta, número de ramos secundários por planta, número de cápsulas por planta e produção de sementes por planta, sugerem que a seleção com base nestas características pode ser eficaz. Em relação ao desempenho per se, os seguintes acessos podem ser explorados em cultivo comercial: Shweta (5,41 g), Gaurav (5,07 g) e EX-3-3 (4,77 g) para produção de sementes; e Shubhra (45,09%), Mukta (44,94%), Laxmi-27 (45,06%) e Shweta (44,25%) para teor de óleo. O perfil químico dos ácidos graxos obtido no presente trabalho pode fornecer uma plataforma para a seleção de acessos para o melhoramento genético de sementes de linhaça

Feeding, survival, and reproduction of two populations of Eurytemora (Copepoda) exposed to local toxic cyanobacteria

Studying the responses of crustacean zooplankton to harmful algal blooms is important for understanding changes in lower food webs following eutrophication in the Laurentian Great Lakes and other regions around the world. Here we examine responses to toxic cyanobacteria by crustacean copepods of the genus Eurytemora from eutrophic coastal regions of Lake Michigan (Green Bay) and the Baltic Sea (Gulf of Finland). We measured grazing, survivorship, reproduction, and juvenile (nauplius) size in short-term laboratory experiments. Females were incubated with representative non-toxic food and mixtures of non-toxic food with either cyanobacteria or cyanobacteria filtrate. Eurytemora from both locations were affected negatively by cyanobacteria filtrates, even with non-toxic food available. Eurytemora carolleeae from Green Bay exhibited reduced grazing rates when exposed to filtrates, but this effect was not observed when animals were fed the cyanobacteria and filtrate along with non-toxic food. Eurytemora sp. from the Baltic Sea given filtrates and non-toxic food also exhibited decreased grazing rates, as well as decreased adult survival and nauplius size. Similarly, when cyanobacterial cells were included along with filtrate and non-toxic food these effects were not observed. Our results also demonstrated a significant trade-off between offspring quantity and quality for both groups of animals, being more pronounced when food quality was manipulated by the presence of cyanobacterial cells. These findings further our knowledge of how a widely distributed group like Eurytemora may respond in the face of changing local selection pressures from natural and anthropogenic stressors. (C) 2017 International Association for Great Lakes Research. Published by Elsevier B.V. All rights reserved.Peer reviewe

Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS

The formation of acquired drug resistance is a major reason for the failure of anti-cancer therapies after initial response. Here, we introduce a novel model of acquired oxaliplatin resistance, a sub-line of the non-MYCN-amplified neuroblastoma cell line SK-N-AS that was adapted to growth in the presence of 4000 ng/mL oxaliplatin (SK-N-ASrOXALI4000). SK-N-ASrOXALI4000 cells displayed enhanced chromosomal aberrations compared to SK-N-AS, as indicated by 24-chromosome fluorescence in situ hybridisation. Moreover, SK-N-ASrOXALI4000 cells were resistant not only to oxaliplatin but also to the two other commonly used anti-cancer platinum agents cisplatin and carboplatin. SK-N-ASrOXALI4000 cells exhibited a stable resistance phenotype that was not affected by culturing the cells for 10 weeks in the absence of oxaliplatin. Interestingly, SK-N-ASrOXALI4000 cells showed no cross resistance to gemcitabine and increased sensitivity to doxorubicin and UVC radiation, alternative treatments that like platinum drugs target DNA integrity. Notably, UVC-induced DNA damage is thought to be predominantly repaired by nucleotide excision repair and nucleotide excision repair has been described as the main oxaliplatin-induced DNA damage repair system. SK-N-ASrOXALI4000 cells were also more sensitive to lysis by influenza A virus, a candidate for oncolytic therapy, than SK-N-AS cells. In conclusion, we introduce a novel oxaliplatin resistance model. The oxaliplatin resistance mechanisms in SK-N-ASrOXALI4000 cells appear to be complex and not to directly depend on enhanced DNA repair capacity. Models of oxaliplatin resistance are of particular relevance since research on platinum drugs has so far predominantly focused on cisplatin and carboplatin

Biochemical characterization based on SDS-page analysis and correlation among traits in opium poppy (Papaver somniferum L.) germplasm

This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard’s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa’s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain

Genetic interrelationship among nutritional and quantitative traits in the vegetable amaranth

The present investigation was conducted to elucidate the interrelationship among various agronomic andquality traits and their direct and indirect effect on foliage yield in 39 distinct cultivars of vegetable amaranth (A. tricolor).Among the agronomic traits, plant height and number of inflorescence exhibited significant positive association with foliageyield, while chlorophyll a, chlorophyll b, carotenoid, fiber and ascorbic acid were positively correlated with foliage yield.Chlorophyll a and chlorophyll b exhibited significant positive association with carotenoid, fiber and ascorbic acid. Ascorbicacid was positively correlated with fiber and carotenoid. Protein was associated with plant height, branches per plant and 500seed weight. Chlorophyll a, carotenoid and inflorescence length revealed high positive direct effect on foliage yield, whilebranches plant-1, leaf size, seed yield, chlorophyll b, moisture content and ascorbic acid showed negative path coefficient withfoliage yield. Suitable traits have been marked out to enhance foliage yield in vegetable amaranth

Effect of auxin and gibberellic acid on growth and yield components of linseed (Linum usitatissimum L.)

The commercial importance of linseed (Linum usitatissimum L.) has attracted breeders to increase its seed yield usingvarious breeding approaches. Plant growth regulators (PGRs) have a significant role in enhancing yield and its related traits in linseed.In the present study, two plant growth hormones, auxin and gibberellic acid, were applied individually, as well as in combinations, inorder to study their effect on yield and its components in “Neelam”, which is a high yielding variety of linseed. A comparative studywas done under pot and field condition. A combined dose of auxin (1.0 mg L-1) and gibberellin (200 mg L-1) is recommended for theenhancement of seed yield, whereas a 0.5 mg L-1 dose of auxin is recommended for the enhancement of vegetative growth. It was concludedthat the plant growth regulators can be successfully employed to enhance the yield in this economically important oil seed crop