Josephson current in carbon nanotubes with spin-orbit interaction

We demonstrate that curvature-induced spin-orbit (SO) coupling induces a $0-\pi$ transition in the Josephson current through a carbon nanotube quantum dot coupled to superconducting leads. In the non-interacting regime, the transition can be tuned by applying parallel magnetic field near the critical field where orbital states become degenerate. Moreover, the interplay between charging and SO effects in the Coulomb Blockade and cotunneling regimes leads to a rich phase diagram with well-defined (analytical) boundaries in parameter space. Finally, the 0 phase always prevails in the Kondo regime. Our calculations are relevant in view of recent experimental advances in transport through ultra-clean carbon nanotubes.Comment: 4 (main text) + 10 (appendices) pages, 3 figure

Magnetic-field instability of Majorana modes in multiband quantum wires

We investigate the occurrence of Majorana modes in semiconductor quantum wires in close proximity with a superconductor and when both, Rashba interaction and magnetic field, are present. We consider long, but finite, multiband wires (namely, planar wires with dimensions $L_x\gg L_y$). Our results demonstrate that interband mixing coming from Rashba spin orbit term hybridizes Majorana pairs originating from different transverse modes while simultaneously closing the effective gap. Consequently, multiple Majorana modes do not coexist in general. On the contrary, Majorana physics is robust provided that only one single transverse mode contributes with a Majorana pair. Finally, we analyse the robustness of Majorana physics with respect to magnetic orbital effects.Comment: 5 pages, 4 figure

Fractionation of the insoluble brain proteins with acrylamide electrophoresis

A method for fractionating the insoluble proteins of the brain with polyacrylamide electrophoresis is described. The procedure consists of dissolving the membrane proteins in a solution of urea, alkali, mercapto-ethanol, and Triton, and then conducting the separation in gels containing urea and Triton in discontinuous acidic buffers. As many as 30 sharp bands are discernable in the gel out of an insoluble brain fraction. The reproducibility of the band pattern is demonstrable in a gel slab system.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32783/1/0000156.pd

Electrophoretic studies on solubilized proteins of goldfish brain

Soluble proteins from homogenates of various regions of goldfish brain have been separated by gel electrophoresis techniques. Distribution of radioactive proteins formed following injection of a labeled amino acid was studied by direct autoradiography of gel slabs. While dye staining and labeling patterns of electrophoretically separated proteins did not vary among brain regions examined, there were marked differences in the positionh of stained bands and those detected autradiographically, suggesting diverse rates of metabolism of these soluble proteins. Unlike the protein staining and labeling patterns, the distribution of multiple electrophoretic bands of solubilized acetylcholinesterase varied among the 5 brain regions examined. Protein bands detected either by dye staining, autoradiography or by acetylcholinesterase activity did not vary measurably as a result of shock-avoidance training of the goldfish. In further studies with DFP, apparent turnover rates for the multiple forms of acetylcholinesterase in a detergent extract of whole brain were determined.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33722/1/0000234.pd

Autoradiography with acrylamide gel slab electrophoresis

A method for electrophoresis and autoradiography with a multisample gel slab system has been described. The method obviates slicing of the gel, retains the high degree of resolution of bands in the autoradiogram, and enables comparison of protein and radioactivity patterns of many samples under essentially identical conditions. Within a limited range, a linear relation is obtained between radioactivity and optical density in the autoradiogram, as well as between protein concentration and optical density in the gel.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32986/1/0000370.pd

Drosophila expressing human SOD1 successfully recapitulates mitochondrial phenotypic features of familial amyotrophic lateral sclerosis

Mitochondrial pathology is a seminal pathogenic hallmark of familial amyotrophic lateral sclerosis (FALS) which is extensively manifested by human patients and mutant SOD1G93A mammalian models. Rodents expressing human FALS-associated mutations successfully mimic several human disease features; although they are not as amenable to genetic and therapeutic compound screenings as non-mammalian models. In this study, we report a newly generated and characterized Drosophila model that expresses human SOD1G93A in muscle fibers. Presence of SOD1G93A in thoracic muscles causes mitochondrial pathology and impairs normal motor behavior in these flies. Use of this new FALS-24B-SOD1G93A fly model holds promise for better understanding of the mitochondrial affectation process in FALS and for the discovery of novel therapeutic compounds able to reverse mitochondrial dysfunction in this fatal disease

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Predictors of NOAC versus VKA use for stroke prevention in patients with newly diagnosed atrial fibrillation: Results from GARFIELD-AF.

INTRODUCTION: A principal aim of the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) was to document changes in treatment practice for patients with newly diagnosed atrial fibrillation during an era when non-vitamin K antagonist oral anticoagulants (NOACs) were becoming more widely adopted. In these analyses, the key factors which determined the choice between NOACs and vitamin K antagonists (VKAs) are explored. METHODS: Logistic least absolute shrinkage and selection operator regression determined predictors of NOAC and VKA use. Data were collected from 24,137 patients who were initiated on AC ± antiplatelet (AP) therapy (NOAC [51.4%] or VKA [48.6%]) between April 2013 and August 2016. RESULTS: The most significant predictors of AC therapy were country, enrolment year, care setting at diagnosis, AF type, concomitant AP, and kidney disease. Patients enrolled in emergency care or in the outpatient setting were more likely to receive a NOAC than those enrolled in hospital (OR 1.16 [95% CI: 1.04-1.30], OR: 1.15 [95% CI: 1.05-1.25], respectively). NOAC prescribing seemed to be favored in lower-risk groups, namely, patients with paroxysmal AF, normotensive patients, and those with moderate alcohol consumption, but also the elderly and patients with acute coronary syndrome. By contrast, VKAs were preferentially used in patients with permanent AF, moderate to severe kidney disease, heart failure, vascular disease, and diabetes and with concomitant AP. CONCLUSION: GARFIELD-AF data highlight marked heterogeneity in stroke prevention strategies globally. Physicians are adopting an individualized approach to stroke prevention where NOACs are favored in patients with a lower stroke risk but also in the elderly and patients with acute coronary syndrome