Проблеми конструювання об'єктів в комп'ютерних системах

Конспект лекцій призначено для підготовки докторів філософії зі спеціальностей "Комп'ютерні науки", "Прикладна механіка"

An Accessible City An investigation using GIS into the potential for further development of the proposed major cycleways network for Christchurch, New Zealand

As the future of the world’s oil reserves becomes progressively more uncertain, it is becoming increasingly important that steps are taken to ensure that there are viable, attractive alternatives to travel by private motor vehicle. As with many of New Zealand’s major urban centres, Christchurch is still exceptionally reliant on private motor vehicles; although a significant proportion of the population indicate that they would like to cycle more, cycling is still an underutilised mode of transport. Following a series of fatal earthquakes that struck the city in 2010 and 2011, there has been the need to significantly redevelop much of the city’s horizontal infrastructure – subsequently providing the perfect platform for significant changes to be made to the road network. Many of the key planning frameworks governing the rebuild process have identified the need to improve Christchurch’s cycling facilities in order to boost cycling numbers and cyclist safety. The importance of considering future growth and travel patterns when planning for transport infrastructure has been highlighted extensively throughout literature. Accordingly, this study sought to identify areas where future cycle infrastructure development would be advantageous based on a number of population and employment projections, and likely future travel patterns throughout the city. Through the use of extensive GIS analysis, future population growth, employment and travel patterns for Christchurch city were examined in order to attain an understanding of where the current proposed major cycleways network could be improved, or extended. A range of data and network analysis were used to derive likely travel patterns throughout Christchurch in 2041. Trips were derived twice, once with a focus on simply finding the shortest route between each origin and destination, and then again with a focus on cyclist safety and areas where cyclists were unlikely to travel. It was found that although the proposed major cycleways network represents a significant step towards improving the cycling environment in Christchurch, there are areas of the city that will not be well serviced by the current proposed network in 2041. These include a number of key residential growth areas such as Halswell, Belfast and Prestons, along with a number of noteworthy key travel zones, particularly in areas close to the central city and key employment areas. Using network analysis, areas where improvements or extensions to the proposed network would be most beneficial were identified, and a number of potential extensions in a variety of areas throughout the city were added to the network of cycle ways. Although it has been found that filling small gaps in the network can have considerable positive outcomes, results from the prioritisation analysis suggested that initially in Christchurch demand is likely to be for more substantial extensions to the proposed major cycleways network

The Dutch Parelsnoer Institute - Neurodegenerative diseases; methods, design and baseline results

Background: The is a collaboration between 8 Dutch University Medical Centers in which clinical data and biomaterials from patients suffering from chronic diseases (so called "Pearls") are collected according to harmonized protocols. The Pearl Neurodegenerative Diseases focuses on the role of biomarkers in the early diagnosis, differential diagnosis and in monitoring the course of neurodegenerative diseases, in particular Alzheimer's disease. Methods: The Pearl Neurodegenerative Diseases is a 3-year follow-up study of patients referred to a memory clinic with cognitive complaints. At baseline, all patients are subjected to a standardized examination, including clinical data and biobank materials, e.g. blood samples, MRI and cerebrospinal fluid. At present, in total more than 1000 patients have been included, of which cerebrospinal fluid and DNA samples are available of 211 and 661 patients, respectively. First descriptives of a subsample of the data (n = 665) shows that patients are diagnosed with dementia (45%), mild cognitive impairment (31%), and subjective memory complaints (24%). Discussion: The Pearl Neurodegenerative Diseases is an ongoing large network collecting clinical data and biomaterials of more than 1000 patients with cognitive impairments. The project has started with data analyses of the baseline characteristics and biomarkers, which will be the starting point of future specific research questions that can be answered by this unique dataset

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance

The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher temperatures are reached. Herein we present a novel Hot Start activation approach in PCR where primers contain one or two thermolabile, 4-oxo-1-pentyl (OXP) phosphotriester (PTE) modification groups at 3′-terminal and 3′-penultimate internucleotide linkages. Studies demonstrated that the presence of one or more OXP PTE modifications impaired DNA polymerase primer extension at the lower temperatures that exist prior to PCR amplification. Furthermore, incubation of the OXP-modified primers at elevated temperatures was found to produce the corresponding unmodified phosphodiester (PDE) primer, which was then a suitable DNA polymerase substrate. The OXP-modified primers were tested in conventional PCR with endpoint detection, in one-step reverse transcription (RT)–PCR and in real-time PCR with SYBR Green I dye and Taqman® probe detection. When OXP-modified primers were used as substitutes for unmodified PDE primers in PCR, significant improvement was observed in the specificity and efficiency of nucleic acid target amplification

Sex Pheromone Evolution Is Associated with Differential Regulation of the Same Desaturase Gene in Two Genera of Leafroller Moths

Chemical signals are prevalent in sexual communication systems. Mate recognition has been extensively studied within the Lepidoptera, where the production and recognition of species-specific sex pheromone signals are typically the defining character. While the specific blend of compounds that makes up the sex pheromones of many species has been characterized, the molecular mechanisms underpinning the evolution of pheromone-based mate recognition systems remain largely unknown. We have focused on two sets of sibling species within the leafroller moth genera Ctenopseustis and Planotortrix that have rapidly evolved the use of distinct sex pheromone blends. The compounds within these blends differ almost exclusively in the relative position of double bonds that are introduced by desaturase enzymes. Of the six desaturase orthologs isolated from all four species, functional analyses in yeast and gene expression in pheromone glands implicate three in pheromone biosynthesis, two Δ9-desaturases, and a Δ10-desaturase, while the remaining three desaturases include a Δ6-desaturase, a terminal desaturase, and a non-functional desaturase. Comparative quantitative real-time PCR reveals that the Δ10-desaturase is differentially expressed in the pheromone glands of the two sets of sibling species, consistent with differences in the pheromone blend in both species pairs. In the pheromone glands of species that utilize (Z)-8-tetradecenyl acetate as sex pheromone component (Ctenopseustis obliquana and Planotortrix octo), the expression levels of the Δ10-desaturase are significantly higher than in the pheromone glands of their respective sibling species (C. herana and P. excessana). Our results demonstrate that interspecific sex pheromone differences are associated with differential regulation of the same desaturase gene in two genera of moths. We suggest that differential gene regulation among members of a multigene family may be an important mechanism of molecular innovation in sex pheromone evolution and speciation