Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe

Archaeological evidence indicates that pig domestication had begun by ~10,500 y before the present (BP) in the Near East, and mitochondrial DNA (mtDNA) suggests that pigs arrived in Europe alongside farmers ~8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local Euro-pean wild boars, although it is also possible that European wild boars were domesticated independently without any genetic con-tribution from the Near East. To test these hypotheses, we obtained mtDNA sequences from 2,099 modern and ancient pig samples and 63 nuclear ancient genomes from Near Eastern and European pigs. Our analyses revealed that European domestic pigs dating from 7,100 to 6,000 y BP possessed both Near Eastern and European nuclear ancestry, while later pigs possessed no more than 4% Near Eastern ancestry, indicating that gene flow from European wild boars resulted in a near-complete disappearance of Near East ancestry. In addition, we demonstrate that a variant at a locus encoding black coat color likely originated in the Near East and persisted in European pigs. Altogether, our results indicate that while pigs were not independently domesticated in Europe, the vast majority of human-mediated selection over the past 5,000 y focused on the genomic fraction derived from the European wild boars, and not on the fraction that was selected by early Neolithic farmers over the first 2,500 y of the domestication process

Withers height of pig - Sus scrofa domestica L. 1758, domestic cow - Bos taurus L. 1758 and sheep - Ovis aries L. 1758 at the “Gornja šuma” archaeological site (Novi Sad)

In spring 2012, osteological material was collected at the “Gornja Šuma” site (site no. 47), located in the territory of Novi Sad, and it was dated to the early 9th century. The withers heights of pig - Sus scrofa domestica, domestic cow - Bos taurus and sheep - Ovis aries, as the three most dominant species at this archaeological site, were analysed based on the length of bones and according to various authors [Boessneck 1956; Zalkin 1960; Matolcsi 1970; Teichert 1975]. It was determined that in these three species the withers heights mostly corresponded to the data from the Middle Ages

The ratio of domestic and wild animals at Neolithic sites in Vojvodina (Serbia)

Based on the results of the vertebrate fauna research from 10 Neolithic archaeological sites in Vojvodina (Serbia), two of which belong to Kőrös culture, 7 to Starčevo culture, and one to Vinča culture, the proportional contribution of domestic and wild animals was analysed. These sites were approximately dated between 6000 and 3200 BC. The smallest proportion of domestic animals was recorded at the sites of Golokut-Vizić and Nosa Biserna Obala, while the biggest one at the sites of Prosine-Pećinci, Zlatara-Ruma and Kudoš-Šašinci. A small proportion of domestic animals at Nosa Biserna Obala shows that the animal husbandry was only just at the beginning, and a high proportion of wild animals testifies about the importance of hunting in economy. These are the characteristics of settlements of Kőrös culture, where goats and sheep dominate among domestic animals. Low proportion of domestic and high proportion of wild animals were recorded at the site of Golokut which, like most of the described sites in this paper, belongs to the Middle Neolithic; this is not characteristic for Starčevo culture and it testifies that hunting was much more important than animal husbandry. What is characteristic for settlements of Starčevo culture is the domination of oxen in the total vertebrate fauna and among domestic animals. At the site of Donja Branjevina-Deronje, the settlement which belongs to Starčevo culture as well, goats and sheep have the biggest proportional contribution. The only analysed set­tlement in this paper which belongs to the Early Neolithic (Vinča culture) is Gomolava - Hrtkovci where domestic animals dominate, oxen being the most numerous ones

Ornitofauna from the archaeological sites in Vojvodina (Serbia)

After decades-long vertebrate fauna research, out of 42 archaeological sites in Vojvodina (Serbia) from different periods ranging from the Neolithic to the Middle Ages, remains of birds were registered at 17 sites (4 from the Neolithic, 1 from the Early Iron Age, 7 from the Late Iron Age, 5 from the Roman Period, 1 from the Migration Period, and 4 from the Middle Ages). A total of 14 species and 4 genera were registered for this vertebrate class. The richest ornithofauna is from the Neolithic, where 9 species and 3 genera were registered. The Migration and Medieval periods are next with 4 registered species and one genus each. There were 3 species registered from the Roman Period, and 2 species from the Late Iron Age. The poorest ornitofauna was registered from the Early Iron Age, only one species

Vertebrate fauna of the early and late iron ages in Vojvodina (Serbia)

Based on current published and unpublished research results, a total of 34 vertebrate species from 4 classes have been registered at 9 archaeological sites from the Early Iron Age in Vojvodina (Serbia). The most numerous one is the mammal class (Mammalia) with 22 species, then osteichthyes class (Osteichthyes) with 10 species, while birds (Aves) and reptiles (Reptilia) are repsented with one species each. From the Late Iron Age, at 14 archaeological sites, a total of 21 species were registered, of which 16 belong to the mammal class (Mammalia), birds (Aves) are represented by 2 species, and osteichthyes (Osteichthyes) by 3 species

Palaeopathological study of Cattle and Horse bone remains of the Ancient Roman city of Sirmium (Pannonia/Serbia)

This paper considers the observation of pathological changes in animal skeletal remains from four archaeological sites in Sirmium and one rustic villa (Vranj) located near the city. Before the pathological analysis, skeletal elements, as well as insight of taxonomic and age of animals were determined. 13599 bones or bone fragments were examined. In 72 specimens various abnormal changes have been observed. Most of the pathological changes were present in skeletal remains of cattle and horses. Macroscopic and radiographic analysis of pathological changes in the animal bones established that the lesions had proliferative and chronic character. Proliferative changes in the bones of cattle and horses indicate that these animals were used as draught animals and/or carrying cargo. Considering that most of the horses skeletal remains are from the Hippodrome, it is very possible that they were animals for amusement purposes in ancient city of Sirmium

Erratum: Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe

The authors note that the affiliation for Alexandros Triantafyllidis and Panoraia Alexandri should be listed as Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; and that the affiliation for Rose-Marie Arbogast should be listed as CNRS UMR 7044, Maison interuniversitaire des sciences de l'Homme, F-67083 Strasbourg Cedex, France. The corrected author and affiliation lines appear below. The online version has been corrected.</p

Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe

Archaeological evidence indicates that pig domestication had begun by ∼10,500 y before the present (BP) in the Near East, and mitochondrial DNA (mtDNA) suggests that pigs arrived in Europe alongside farmers ∼8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local European wild boars, although it is also possible that European wild boars were domesticated independently without any genetic contribution from the Near East. To test these hypotheses, we obtained mtDNA sequences from 2,099 modern and ancient pig samples and 63 nuclear ancient genomes from Near Eastern and European pigs. Our analyses revealed that European domestic pigs dating from 7,100 to 6,000 y BP possessed both Near Eastern and European nuclear ancestry, while later pigs possessed no more than 4% Near Eastern ancestry, indicating that gene flow from European wild boars resulted in a near-complete disappearance of Near East ancestry. In addition, we demonstrate that a variant at a locus encoding black coat color likely originated in the Near East and persisted in European pigs. Altogether, our results indicate that while pigs were not independently domesticated in Europe, the vast majority of human-mediated selection over the past 5,000 y focused on the genomic fraction derived from the European wild boars, and not on the fraction that was selected by early Neolithic farmers over the first 2,500 y of the domestication process