20 research outputs found
Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy
- Author
- Aahlin EK
- Aamer A
- Aaniana K
- Ababneh A
- Abaliksta T
- Abantanga F
- Abantanga F
- Abbas A
- Abbas M
- Abbasy J
- Abbo O
- Abbo O
- Abbott T
- Abbouch M
- Abd El Galeel A
- Abd El Hameed OS
- Abd El Hamid N
- Abd El Salam Y
- Abd El Slam M
- Abd El-Salam F
- Abd Elrasoul Y
- Abd-Elmawla M
- Abd-Elrasoul Y
- Abdallah E
- Abdallah E
- Abdel-Aty A
- Abdel-Hameed N
- Abdel-Kader SM
- Abdel-Wahab NYE
- Abdelaal A
- Abdelaty M
- Abdelazeam AR
- Abdelazim G
- Abdelaziz MA
- Abdelbadeai K
- Abdelfatah A
- AbdelFattah I
- Abdelgelil A
- Abdelghany S
- Abdelhady S
- Abdelhamed R
- Abdelhamid A
- Abdelhaq A
- Abdelkader M
- Abdelkader M
- Abdelkader O
- Abdelkareem A
- Abdelkhalek M
- Abdelmageed E
- Abdelmotaleb I
- Abdelraheim M
- Abdelraouf AM
- Abdelrouf DM
- Abdelshafy M
- Abdelshakour M
- Abdrabou AK
- Abdul-Latif S
- Abdulaziz H
- Abdulaziz M
- Abdulbagi O
- Abdulgawad M
- Abdulhakeem EM
- Abdullah AAN
- Abdullah MA
- Abdullah N
- Abdullah NAN
- Abdullah NAN
- Abdullahi L
- Abdurrazzaaq A
- Abebe NS
- Abebe NS
- Abem OE
- Aber A
- Abington E
- Abiola O
- Aboarab A
- Aboelella M
- Aboelmagd O
- Aboelsoud MR
- Aboraya M
- Aboraya M
- Abouelnasr A
- Aboul-Naga MS
- Abouzahra A
- Abouzaid A
- Abozaid M
- Abozeid K
- Abozyed MAF
- Abu Qumbos A
- Abu-toyour M
- Abubakr M
- Abudeeb H
- Abuowda Y
- Abuqwaider E
- Abusalem L
- Abuseif M
- Abushamleh S
- Acharya P
- Ackom E
- Acquah E
- Adam A
- Adam N
- Adams R
- Adamu A
- Adawi I
- Adawi M
- Adebanjo A
- Adebanjo A
- Adebola O
- Adedeji A
- Adekoya A
- Adel A
- Adel A
- Adel BE
- Adel I
- Adella FJ
- Adelshone A
- Ademuyiwa A
- Ademuyiwa A
- Ademuyiwa AO
- Ademuyiwa AO
- Ademuyiwa AO
- Ademuyiwa AO
- Adenekan B
- Adeniran J
- Adeniran J
- Adeniyi A
- Adeniyi A
- Adesanya O
- Adesanya O
- Adesina A
- Adesina A
- Adesuyi A
- Adeyeye A
- Adeyeye A
- Adeyeye A
- Adham W
- Adhitama N
- Adil M
- Adisa AO
- Adisa AO
- Adu-Aryee N
- Afana S
- Afandy A
- Afifi AM
- Afzal M
- Agalianos C
- Agalianos C
- Agarwal A
- Agarwal A
- Agarwal A
- Agbedinu K
- Agius M
- Aglan A
- Aguado Suarez N
- Aguilar-Jimenez J
- Aguilera M
- Aguilera ML
- Aguilera ML
- Aguilera ML
- Aguilera O
- Aguilera-Arevalo M-L
- Ahlonsou G
- Ahlqvist S
- Ahlqvist S
- Ahlqvist S
- Ahmed A
- Ahmed A
- Ahmed A
- Ahmed EA
- Ahmed H
- Ahmed H
- Ahmed H
- Ahmed LAM
- Ahmed S
- Ahmed S
- Ahmed SM
- Ahounou EYS
- Aigrain Y
- Aiyaman C
- Ajah J
- Ajai O
- Ajao A
- Ajao AE
- Ajiboye A
- Akatibo E
- Akeel N
- Akhtar M
- Akoto E
- Akpenyi O
- Al Amin S
- Al Attar SM
- Al Awwad S
- Al Barrawi FE
- Al Bastawis F
- Al Meligy A
- Al Rafati AAR
- Al Raouf EA
- Al Saied G
- Al Subaie N
- Al-Aarag AA-R
- Al-Azraqi IAA
- Al-Bahrani A
- Al-Buhaisi A
- Al-Dakka E
- Al-Faifi JJ
- Al-faqawi M
- Al-farram H
- Al-Hameedi HII
- Al-Hasani RKMJ
- Al-khatib A
- Al-Mallah A
- Al-Marakby D
- Al-Mousa M
- Al-Nahrawi S
- Al-Obaedi O
- Al-saqqa S
- Al-Saqqa SW
- Al-Saqqa SW
- Al-shammari A
- Al-Slaibi I
- Al-taher T
- Alaael-Dein S
- Alada M
- Alahmady O
- Alakaloko F
- Alakaloko F
- Alaloul E
- Alamin S
- Alamoudi M
- Alamoudi U
- Albeladi B
- Albiety A
- Alcca Ticona W
- Aldalaq A
- Aldhafeeri S
- Aledrisy M
- Alejos Bermudez YE
- Alexander P
- Alexander P
- Alexander P
- Alexandre C
- Alfarsi MA
- Alghamdi A
- Alghamdi B
- Alghamdi M
- Alghamdi MSA
- Algohary O
- Alhabli I
- Alhady K
- Alhammali T
- Alhassan B
- Alhazmi W
- Alhendy M
- Alherz M
- Ali A
- Ali A
- Ali A
- Ali CDM
- Ali E
- Ali FM
- Ali H
- Ali S
- Ali SAWEW
- Ali SS
- Ali THA
- Ali W
- Alihonou T
- Alimi Q
- Alis H
- Aliyu H
- Aliyu M
- Aliyu S
- Aljboor K
- Aljiffry M
- Aljiffry M
- Aljohani E
- Aljohani E
- Aljohani S
- Alkaaki A
- Alkammash A
- Alkelani M
- Alkhabbaz D
- Alkhouri M
- Allam M
- Allam M
- Allam Y
- Allegri A
- Allegri A
- Allen JLY
- Allott R
- Almallah A
- Almoflihi A
- Almuallem S
- Almy J
- Alnaby MH
- Alnawam E
- Alnuqaydan SA
- Alnusairat A
- Alomar IN
- Alqahtani E
- Alqahtani S
- Alrahawy M
- Alrajraji M
- Alrifaie A
- AlRowais M
- Alsaggaf M
- Alsaggaf MU
- Alsaggaf MU
- Alser OH
- Alshareef R
- Altaf A
- Altaf A
- Altamimi A
- Altamimi A
- Altamimi A
- Altamimi A
- Altamini A
- Altarayra M
- Althwainy A
- Altibi A
- Altinel Y
- Altinel Y
- Altoe F
- Altwigry AM
- Altwijri A
- Alvarado Jaramillo R
- Alvarez Barreda LM
- Alvi AR
- Alwafai MG
- Aly AK
- Aly MYM
- Alyacoubi S
- Alyahya R
- Alyami R
- Alyami R
- AlYoussef I
- Alzahrani A
- Alzahrani M
- Alzahrani M
- Alzayat T
- Aman NF
- Amandito R
- Amandito R
- Amandito R
- Amandito R
- Ambrona-Zafra D
- Ambrozeviciute V
- Ameen M
- Amer MA
- Amin HAA
- Amin J
- Amin J
- Amjad M
- Ammar M
- Ammar MS
- Amoah M
- Amoako J
- Amoako PT
- Amoako-Boateng M
- Amole I
- Amreia M
- Amreia M
- Anandkumar D
- Anderson D
- Anderson F
- Anderson R
- Anderson-Knight H
- Andersson L
- Andika FFA
- Andreotti D
- Andres Garcia-Marin J
- Andress C
- Andriola V
- Ang YL
- Angelieri D
- Anis E
- Ansaloni L
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- Ansaloni L
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- Antar S
- Antezana D
- Anthoulakis C
- Anthoulakis C
- Antoniozzi A
- Anwar AW
- Anwar N
- Anwer W
- Anyanwu L-J
- Anyanwu L-J
- Aonzo P
- Appeadu-Mensah W
- Appiah EK
- Aql S
- Aquilino F
- Arachchi PP
- Arachchige LAJJ
- Arafa O
- Arafa S
- Arango MCM
- Arango MCM
- Aranzabal Durand SY
- Araujo R
- Arcudi C
- Ardley R
- Arenas A
- Arevalo Azmitia JR
- Arias Pacheco RD
- Arman S
- Arman S
- Armando ChunguiBravo J
- Armellini A
- Arnaud A
- Arnaud AP
- Arnaud AP
- Arnaud AP
- Arroyo Basto CA
- Arshad MM
- Arslan E
- Arsyad HM
- Arthur T
- Arulampalam T
- Aruldas N
- Asal M
- Asar YH
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- Asele FA
- Ashour A
- Ashour A
- Ashour K
- Ashraf SF
- Ashtari M
- Ashton A
- Asi F
- Asilah SMD
- Assal A
- Assouto PA
- Asturia S
- Asumah H
- Ata A
- Ata AAA
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- Atiyeh A
- Atkins ET
- Atkinson K
- Atobatele K
- Attallah NA
- Attard M
- Attia A
- Attia AM
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- Aung KP
- Austraite A
- Aviles S
- Avoka A
- Awad I
- Awad SA
- Awadallah S
- Awuku-Asabre J
- Ayad S
- Ayandipo O
- Ayandipo OO
- Aydin MC
- Ayyar S
- Aziz DNA
- Aziz MRA
- Aziz NA
- Azizeldine MG
- Azmitia Mendizabal CA
- Azodo IA
- Azzie G
- Azzie G
- Azzis O
- Baba S
- Bachri H
- Badawy M
- Badenjki MA
- Badicel M
- Badr A
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- Badwi NM
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- Badwy A
- Bahar S
- Baheeg M
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- Baillie K
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- Baird A
- Bakhaidar M
- Baki BE
- Bakr A
- Bakry HM
- Bala M
- Balalis D
- Baldacchino M
- Balducci G
- Balila RMH
- Balkan AZA
- Ballouhey Q
- Balmaceda R
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- Balogun F
- Baltrunas R
- Baluch F
- Bamford R
- Bamicha A
- Bamicha A
- Banaitis VJ
- Bandi A
- Bandoh D
- Bani-Sadar A
- Banipal GS
- Baraka H
- Barakat SAE
- Barendegere V
- Barker T
- Barkolias C
- Barmayehvar B
- Barnabas GN
- Barnacle J
- Barneo L
- Barrionuevo Ojeda RR
- Barrios E
- Barrios E
- Barros De Castro Segundo LC
- Barroso de Lima CV
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- Basilico S
- Basir ERM
- Bassem M
- Bassit KA
- Basso SMM
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- Batt J
- Bayram S
- Beamon M
- Beasley W
- Beaton C
- Bedada AG
- Bediako-Bowan A
- Behar N
- Beisa V
- Beisa V
- Bekhet AH
- Bekhet NM
- Beleno AEM
- Belkouchi A
- Belli A
- Bello B
- Benammi S
- Benevento A
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- Bennai RM
- Benons N
- Bentounsi Z
- Benyaiche C
- Berti S
- Bertuello I
- Beuran M
- Beuran M
- Beyene R
- Bezerra Morais PA
- Bezzina M
- Bhang A
- Bhangu A
- Bhangu A
- Bhargava A
- Bharj IS
- Bhat S
- Bhatnagar A
- Bhattacharya S
- Bhopal KF
- Bhopal KF
- Bianco F
- Bigaran A
- Billy M
- Bin Adnan A
- Bin Hasnan M
- Bin Ismail MJ
- Bin Mahamood A
- Birindelli A
- Birring A
- Bisset C
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- Blacker S
- Blackwell J
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- Blencowe N
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- Blower E
- Bluelle R
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- Boddy A
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- Breslin R
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- Chandrakumar C
- Chang K
- Chang KY-C
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- Chapman SJ
- Charles A
- Cheah AEZ
- Chen CY
- Chen KY
- Chen T-L
- Chenn R
- Cheong YJ
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- Cheung YHE
- Chew LS
- Chhabra S
- Chibuye C
- Chidambaram S
- Chiesa D
- Chilton G
- Chin PX
- Chin YR
- Chiu S
- Chiu S
- Chiu SMY
- Choi J
- Choi P
- Chong BFHK
- Chong CL
- Chong CL
- Chong CL
- Chong CS
- Chong H-Y
- Chong HY
- Choudhry A
- Chow CYN
- Chowdhury A
- Chowdhury D
- Chu K
- Chun A
- Chun T
- Chung KJ
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- Cirocchi R
- Ciubotaru C
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- Clement KD
- Clements JM
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- Contreras Vergara AL
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- D'amours SK
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- Daniyan M
- Danys D
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- Darweesh M
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- Delforge X
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- Dempster E
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- Denewar A
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- Deputy M
- Der Y
- Dervenis C
- Despotidis V
- Desta S
- Deutschmann P
- Devadasar GH
- Dharamshi HA
- Dhillon D
- Di Franco F
- Di Martino N
- Di Saverio S
- Di Saverio S
- Diab A
- Diaconescu B
- Diaconescu I-B
- Dias S
- Diaz Vico T
- Diaz-Jover P
- Diaz-Zorrilla C
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- Dickfos M
- Dickson L
- Dijan E
- Dimech T
- Din F
- Dindyal S
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- Dione Parreno-Sacdalan M
- Dissanayake T
- Djivoh F
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- Donoghue D
- Dorani RMNR
- Dorisme A
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- El Dahab AA
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- El Gendy A
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- El Jamassi A
- El Kashash A
- El Kholy A
- El Magd AA
- El Mesery S
- El Sayed GS
- El Sayed IM
- El Shoura Y
- El-badawy HA
- El-Dien KS
- El-Dien KS
- El-Din RA
- El-Din SA
- El-Gizawy E
- El-Hamouly AS
- El-Kashef H
- El-Latif NK
- El-Ma'doul AS
- El-Rabaa S
- El-Sagheer N
- El-Sawy A
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- El-Shahat NS
- El-Sheemy H
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- Elabdin HZ
- Elashmawy M
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- Elazoul M
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- Elbanby ES
- Elbatahgy A
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- Elbisomy KH
- Eldafrawy M
- Eldamaty L
- Eldeeb AZ
- Eldeen EA
- Eldin MAB
- EldosoukyMohammed A
- Elebute O
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- Elena G
- Elfarargy A
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- Elfiky M
- Elfil M
- Elfouly N
- Elfouly N
- Elfouly Y
- Elfouly Y
- Elgebaly A
- Elgendy AH
- Elgendy FI
- Elgheriany M
- Elhadad A
- Elhadary NM
- Elhadry S
- Elhalawany E
- Elhamouly S
- Elhelbawy MS
- Elhendawy A
- Elhendawy AOA
- Elhoseny G
- Elhusseiny AAE
- Elizabeth Lopez C
- Elkadsh I
- Elkady F
- Elkelany A
- Elkelany A
- Elkelany A
- Elkelany A
- Elkhadrawi M
- Elkhalek EA
- Elkholy A
- Elkholy SS
- Elkolaly SS
- Elkorashy AM
- Ellison Q
- Elmashala A
- Elmasry M
- Elmelegy R
- Elmihy S
- Elnagar A
- Elnajjar MA
- Elnemr AE
- Elrazek AA
- Elsabbagh N
- Elsameea AA
- Elsawahly DME
- Elsawy A
- Elsayed M
- Elsayed M
- Elsayed N
- Elsayed NAR
- Elsayed ZM
- Elsebaaye AO
- Elsehimy M
- Elsemelawy R
- Elshaar M
- Elshaer K
- Elshaer M
- Elshaer M
- Elshafay AE
- Elshami M
- Elshanwany S
- Elsharkawy AA
- Elsheikh S
- Elsherbiney S
- Elsherbiny AS
- Elsherif FA
- Elshobary M
- Elsiddig M
- Elsobky S
- Elsorogy DEAA
- Elwaey A
- Elwakil H
- Elwan A
- Elwan A
- Elwy E
- Elzahed N
- Elzayat I
- Elzayat M
- Elzayyat I
- Elzowawi F
- Emadeldin D
- Emadeldin H
- Emara E
- Emile S
- Eng C
- English W
- Esam A
- Escalante Salas MDC
- Espin-Basany E
- Espinoza F
- Essam A
- Essam AA
- Essam M
- Essoun S
- Esteban E
- Estee MM
- Estupinian S
- Etchill E
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- Etienne S
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- Etwire V
- Evajelista CV
- Evans D
- Evans E
- Eysa A
- Eyssartier E
- Ezzat E
- Ezzat T
- Faboya O
- Faccincani R
- Fadel A
- Fahiem A
- Fahim M
- Fahmy MAB
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- Zilinskiene R
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- Zulkifli A
- Zumbuhl L
- Publication venue
- Publication date
- 15/10/2018
- Field of study
Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe
Global disparities in surgeons’ workloads, academic engagement and rest periods: the on-calL shIft fOr geNEral SurgeonS (LIONESS) study
- Author
- Abdelkhalek Mohamed
- Abellán Antonio Melero
- Abelló David
- Abu-Arish Hamdoon
- Acevedo Ana Karen Radilla
- Adamina Michel
- Adamovich Dmitry Mikhailovich
- Addeo Pietro
- Adewunmi Olayinka Lukman
- Aeschbacher Pauline
- Afolabi Akinwale Ibrahim
- Agastra Ervis
- Agresta Ferdinando
- Aguilar Juan Carlos Cardozo
- Ahmed Bensaad
- Ahmed Nauman
- Aimal Aimal Khan
- Aiolfi Alberto
- Akay Omer
- al-Najami Issam
- Al-Shehari Mohammed
- Alconchel Felipe
- Alesina Pier Francesco
- Alhazmi Ahmad
- Ali Mahmoud Ibrahim
- Aliyev Vusal
- Alonso Patricia
- Aloun Ali
- Altinel Yuksel
- Altomare Michele
- Alvarez-Gallego Mario
- Ammendola Michele
- Ammerata Giorgio
- Anania Marco
- Anas Abdelhmed
- Andreuccetti Jacopo
- Andriola Valeria
- Andriola Valeria
- Anelli Ferdinando M.
- Anestiadou Elissavet
- Angrisani Marco
- Antonacci Nicola
- Antonacci Nicola
- Antonelli Amedeo
- Antropoli Carmine
- Aparicio Antonio Carlos Maya
- Apostolou Konstantinos
- Arcudi Claudio
- Aregawi Alazar Berhe
- Argenio Giulio
- Ascari Francesca
- Asfaw Fitsum Terefe
- Athar Rahmatullah
- Atici Semra Demirli
- Au Stephanie
- Augustin Goran
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- Walędziak Maciej
- Wani Ajaz
- Wani Ishfaq Ahmad
- Wani Rauf
- Wassie Chekol
- Wilkins Alex
- Wyroslak-Najs Justyna
- Yallew Gebeyaw Mengist
- Yang Wah
- Yildirim Ali Cihat
- Yildiz Ishak
- Yiu Andrew
- Yiu Andrew
- Younis Muhammad Umar
- Yusoff Ahmad Ramzi
- Zacharis Georgios
- Zafra David Ambrona
- Zaghloul Tarek M.
- Zambon Martina
- Zanus Giacomo
- Zarnescu Narcis Octavian
- Zeb Muhammad
- Zena Alemayehu Shanko
- Zerbo Domenico
- Zuchini Andrés Mendoza
- Zullo Alessia
- Publication venue
- Publication date
- 29/04/2024
- Field of study
: The workload of general surgeons is multifaceted, encompassing not only surgical procedures but also a myriad of other responsibilities. From April to May 2023, we conducted a CHERRIES-compliant internet-based survey analyzing clinical practice, academic engagement, and post-on-call rest. The questionnaire featured six sections with 35 questions. Statistical analysis used Chi-square tests, ANOVA, and logistic regression (SPSS® v. 28). The survey received a total of 1.046 responses (65.4%). Over 78.0% of responders came from Europe, 65.1% came from a general surgery unit; 92.8% of European and 87.5% of North American respondents were involved in research, compared to 71.7% in Africa. Europe led in publishing research studies (6.6 ± 8.6 yearly). Teaching involvement was high in North America (100%) and Africa (91.7%). Surgeons reported an average of 6.7 ± 4.9 on-call shifts per month, with European and North American surgeons experiencing 6.5 ± 4.9 and 7.8 ± 4.1 on-calls monthly, respectively. African surgeons had the highest on-call frequency (8.7 ± 6.1). Post-on-call, only 35.1% of respondents received a day off. Europeans were most likely (40%) to have a day off, while African surgeons were least likely (6.7%). On the adjusted multivariable analysis HDI (Human Development Index) (aOR 1.993) hospital capacity > 400 beds (aOR 2.423), working in a specialty surgery unit (aOR 2.087), and making the on-call in-house (aOR 5.446), significantly predicted the likelihood of having a day off after an on-call shift. Our study revealed critical insights into the disparities in workload, access to research, and professional opportunities for surgeons across different continents, underscored by the HDI
Topics of contemporary computational mathematics
- Author
- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
The Interaction of Activated Integrin Lymphocyte Function-associated Antigen 1 with Ligand Intercellular Adhesion Molecule 1 Induces Activation and Redistribution of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 in T Lymphocytes
- Author
- Akasaka T.
- Akiyama S.K.
- Akiyama T.
- Alfonso Luque
- Arroyo A.G.
- Astier A.
- Avraham S.
- Berendt A.R.
- Carlos Cabañas
- Cary L.A.
- Clark E.A.
- del Pozo M.A.
- del Pozo M.A.
- Dikic I.
- Dransfield I.
- Dransfield I.
- Dustin M.L.
- Earp H.S.
- Francisco Sánchez-Madrid
- Ganju R.K.
- Gates R.E.
- Geiger B.
- Gismondi A.
- Guan J.-L.
- Hamawy M.M.
- Hanks S.K.
- Hanks S.K.
- Herzog H.
- Hiregowdara D.
- Ilic D.
- Joan Brugge
- José Luis Rodrı́guez-Fernández
- Kanner S.B.
- Kanner S.B.
- Kanner S.B.
- Kornberg L.J.
- Kupfer A.
- Lev S.
- Ley S.C.
- Li J.
- Lipfert L.
- Lowin-Kropf B.
- Lub M.
- Luque A.
- Ma E.A.
- Maguire J.E.
- Manuel Gómez
- Marie-Cardine A.
- Matsumoto K.
- Nancy Hogg
- Ohba T.
- Ortlepp S.
- Otey C.A.
- Raja S.
- Ratner S.
- Richardson A.
- Richardson A.
- Rodrı́guez-Fernández J.L.
- Rodrı́guez-Fernández J.L.
- Rodrı́guez-Fernández J.L.
- Rothlein R.
- Sasaki H.
- Schaller M.D.
- Schaller M.D.
- Schaller M.D.
- Schlaepfer D.D.
- Schwartz M.A.
- Serrador J.
- Shattil S.J.
- Singer S.J.
- Sinnett-Smith J.
- Springer T.A.
- Stephens P.
- Stewart M.P.
- Sánchez-Madrid F.
- van Kooyk Y.
- Wacholtz M.C.
- Wilkinson P.C.
- Xiong W.-C.
- Yu H.
- Zheng C.
- Publication venue
- The American Society for Cell Biology
- Publication date
- 01/06/1999
- Field of study
Integrin receptors play a central role in the biology of lymphocytes, mediating crucial functional aspects of these cells, including adhesion, activation, polarization, migration, and signaling. Here we report that induction of activation of the β2-integrin lymphocyte function-associated antigen 1 (LFA-1) in T lymphocytes with divalent cations, phorbol esters, or stimulatory antibodies is followed by a dramatic polarization, resulting in a characteristic elongated morphology of the cells and the arrest of migrating lymphoblasts. This cellular polarization was prevented by treatment of cells with the specific tyrosine kinase inhibitor genistein. Furthermore, the interaction of the activated integrin LFA-1 with its ligand intercellular adhesion molecule 1 induced the activation of the cytoplasmic tyrosine kinases focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK-2). FAK activation reached a maximum after 45 min of stimulation; in contrast, PYK-2 activation peaked at 30 min, declining after 60 min. Upon polarization of lymphoblasts, FAK and PYK-2 redistributed from a diffuse localization in the cytoplasm to a region close to the microtubule-organizing center in these cells. FAK and PYK-2 activation was blocked when lymphoblasts were pretreated with actin and tubulin cytoskeleton-interfering agents, indicating its cytoskeletal dependence. Our results demonstrate that interaction of the β2-integrin LFA-1 with its ligand intercellular adhesion molecule 1 induces remodeling of T lymphocyte morphology and activation and redistribution of the cytoplasmic tyrosine kinases FAK and PYK-2
The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9
- Author
- A Higginbottom
- A Ludwig
- AG Arroyo
- AJ Gearing
- Alvaro Gilsanz
- BF Becker
- C Arduise
- Carlos Cabañas
- Carmen Domínguez
- D Xu
- DR Edwards
- DV Bax
- E Nishi
- Esther M. Lafuente
- F Berditchevski
- F Le Naour
- F Peiretti
- Francisco Sánchez-Madrid
- FW Luscinskas
- G Murphy
- I Gonzalez-Alvaro
- Isidoro González-Alvaro
- JC Montero
- JR Doedens
- K Reiss
- KB Glaser
- KJ Garton
- LF Jackson
- M Canault
- M Miyake
- M Miyake
- M Yanez-Mo
- M Yanez-Mo
- M Yanez-Mo
- MA Lafleur
- Maria Dolores Gutiérrez-López
- María Yáñez-Mó
- MD Gutierrez-Lopez
- ME Hemler
- MS Chen
- N Ouchi
- NL Tsakadze
- O Barreiro
- O Barreiro
- OV Kovalenko
- P Isler
- P Reddy
- Peter N. Monk
- R Mezyk
- R Nishiuchi
- RA Black
- S Charrin
- S Ovalle
- SH Ho
- Susana Ovalle
- T Rabie
- TP Condon
- W Shi
- XJ Zhao
- Y Takahashi
- Y Yan
- Y Zheng
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2011
- Field of study
ADAM17/TACE is a metalloproteinase
responsible for the shedding of the proinflammatory cytokine TNF-a and many other cell surface proteins involved in
development, cell adhesion, migration, differentiation, and
proliferation. Despite the important biological function of
ADAM17, the mechanisms of regulation of its metalloproteinase activity remain largely unknown. We report here that
the tetraspanin CD9 and ADAM17 partially co-localize on
the surface of endothelial and monocytic cells. In situ
proximity ligation, co-immunoprecipitation, crosslinking,
and pull-down experiments collectively demonstrate a direct
association between these molecules. Functional studies
reveal that treatment with CD9-specific antibodies or
neoexpression of CD9 exert negative regulatory effects on
ADAM17 sheddase activity. Conversely, CD9 silencing
increased the activity of ADAM17 against its substrates
TNF-a and ICAM-1. Taken together, our results show that
CD9 associates with ADAM17 and, through this interaction,
negatively regulates the sheddase activity of ADAM17s. This work was supported by
grants BFU2007-66443/BMC and BFU2010-19144/BMC from Ministerio de Ciencia e Innovación, a grant from Fundación de
Investigación Médica Mutua Madrileña and by RETICS Program
RD08/0075-RIER (Red de Inflamacio´n y Enfermedades Reumáticas)
from Instituto de Salud Carlos III (to C.C.), a grant from Fundación de
Investigación Médica Mutua Madrileña (to M.D.G.L.), and grants
PI080794 from Instituto de Salud Carlos III (to M.Y-M) and
SAF2007-60578 from Ministerio de Ciencia e Innovación (to
E.M.L.). M.D.G.L. was supported by a contract associated to grant
SAF2004-01715 from Ministerio de Ciencia e Innovacio´n. S.O. was
supported by an I3P predoctoral Fellowship from Consejo Superior de
Investigaciones Científicas (CSIC) and by a contract associated to
grant BFU2007-66443/BMC from Ministerio de Ciencia e Innovacio´n. A.G. has been supported by a predoctoral Fellowship from
Instituto de Salud Carlos III and by grant BFU2007-66443/BMC from
Ministerio de Ciencia e Innovación.Peer reviewe
The gas companies in Spain, a long-run approach (1842–2018)
- Author
- Alayo J. C.
- Alberte Martínez-López
- Alves de Costa J.
- Arroyo M.
- Arroyo M.
- Arroyo M.
- Ayuntamiento de Madrid
- Ballestero A.
- Barca F. X.
- Bartolomé I.
- Bartolomé I.
- Bel G.
- Bijker W. E.
- Brion R.
- Cardoso de Matos A.
- Carreras A.
- Castro-Valdivia M.
- Correljé A.
- Costa M. T.
- Del Guayo Castiella I.
- Del Guayo I.
- Del Moral J.
- Dirección General de Rentas Públicas.
- Díaz J. M.
- Fernandez A.
- Fernández-Paradas M.
- Fernández-Paradas M.
- Fernández-Paradas M.
- Fernández-Paradas M.
- Fernández-Paradas M.
- Fernández-Paradas M.
- Fàbregas P. A.
- Fàbregas P. A.
- Fàbregas P. A.
- Fàbregas P. A.
- Fàbregas P. A.
- Gao A. M. Z.
- García de la Fuente D.
- Giuntini A.
- Gómez-Ibáñez J. A.
- Jesús Mirás-Araujo
- Jiménez J. C.
- Madureira N. L.
- Maestro G.
- Martos F.
- Martínez A.
- Martínez A.
- Martínez A.
- Matés J. M.
- Mercedes Fernández-Paradas
- Midttun A.
- Moyano F.
- Paquier S.
- Reher D.-S.
- Rodríguez M.
- Romaní M.
- Sudrià C.
- Sudrià C.
- Sudrià C.
- Union International de l’Industrie du Gaz
- Victor D.
- Williot J.-P.
- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
The Src Kinase p56 Up-regulates VLA-4 Integrin Affinity
- Author
- Alon
- Alon
- Arroyo
- August
- Bargatze
- Bargatze
- Bazzoni
- Berlin
- Blystone
- Brenner
- Butcher
- Campbell
- Campbell
- Carmo
- Carr
- Chan
- Chen
- Chen
- Deborah Yablonski
- Denny
- Dustin
- Eitan Winter
- Evans
- Faull
- Gonzalez Garcia
- Grabovsky
- Hardwick
- Hatakeyama
- Humphries
- Ilangumaran
- Imbert
- Jakubowski
- Janes
- Jenkins
- Jin
- Johnston
- Konstantopoulos
- Koretzky
- Laudanna
- Lawrence
- Lim
- Lin
- Ling L. Chen
- Lobb
- Luo
- Luque
- Luscinskas
- Mannion
- Masumoto
- Minami
- Molina
- O'Toole
- O'Toole
- O'Toole
- Page
- Peled
- R. Blake Pepinsky
- Reedquist
- Ronen Alon
- Rose
- Roy Lobb
- Sanchez-Madrid
- Sara W. Feigelson
- Secrist
- Sethi
- Shimizu
- Shimizu
- Shimizu
- Springer
- Stewart
- Straus
- Tanaka
- Ted Yednock
- Valentin Grabovsky
- van Kooyk
- Veillette
- Wang
- Warnock
- Weiss
- Weiss
- Williams
- Xavier
- Yablonski
- Yauch
- Yednock
- Zell
- Zhang
- Publication venue
- 'American Society for Biochemistry & Molecular Biology (ASBMB)'
- Publication date
- Field of study