Characterization of A Novel Avalanche Photodiode for Single Photon Detection in VIS-NIR Range

In this work we investigate operation in the Geiger mode of the new single photon avalanche photo diode (SPAD) SAP500 manufactured by Laser Components. This SPAD is sensitive in the range 400-1000nm and has a conventional reach-through structure which ensures good quantum efficiency at the long end of the spectrum. By use of passive and active quenching schemes we investigate detection efficiency, timing jitter, dark counts, afterpulsing, gain and other important parameters and compare them to the "standard" low noise SPAD C30902SH from Perkin Elmer. We conclude that SAP500 offers better combination of detection efficiency, low noise and timing precision

SREBP-1a activation by HBx and the effect on hepatitis B virus enhancer II/core promoter

Hepatitis B virus (HBV) X protein (HBx) plays an important role in HBV pathogenesis by regulating gene expression. Sterol regulatory element binding protein-1a (SREBP-1a) is a key transcriptional factor for modulating fatty acid and cholesterol synthesis. Here we demonstrated that HBx increased mature SREBP-1a protein level in the nucleus and its activity as a transcription factor. We further showed that the up-regulation of SREBP-1a by HBx occurred at the transcriptional level after ectopic expression and in the context of HBV replication. Deletional analysis using SREBP-1a promoter revealed that the sequence from -436 to -398 in the promoter was required for its activation by HBx. This promoter region possesses the binding sequences for two basic leucine zipper (b-ZIP) transcription factors, namely C/EBP and E4BP4. Mutagenesis of the binding sequences on the SREBP-1a promoter and ectopic expression experiments demonstrated that C/EBPα enhanced SREBP-1a activation by HBx, while E4BP4 had an inhibitory effect. C/EBPα was able to significantly reverse the inhibitory activity of E4BP4 on SREBP-1a promoter. These results demonstrated that HBx activates SREBP-1a activity at the transcription level through a complex mechanism involving two bZIP transcription factors C/EBP and E4BP4 with C/EBP being the dominant positive factor. Finally, we showed that knocking down SREBP-1 abolishes HBV enhancer II/core promoter activation by HBx.Fil: Qiao, Ling. University Of Saskatchewan; CanadáFil: Wu, Qi. University Of Saskatchewan; CanadáFil: Lu, Xinya. University Of Saskatchewan; CanadáFil: Zhou, Yan. University Of Saskatchewan; CanadáFil: Fernández Alvarez, Ana Julia. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ye, Lihong. Nankai University; ChinaFil: Zhang, Xiaodong. Nankai University; ChinaFil: Han, Jihong. Nankai University; ChinaFil: Casado, Marta. Consejo Superior de Investigaciones Cientificas; EspañaFil: Liu, Quiang. University Of Saskatchewan; Canad

A new geometric-and-physics model of milling and an effective approach to medial axis transforms of free-form pockets for high performance machining

Mechanical part quality and productivity depend on many parameters in CNC milling processes, such as workpiece material, cutters, tool paths, feed rate, and spindle speed, etc. To pursue high performance machining, the cutting parameter optimization is in high demand in industry, though it is quite challenge. This innovative research successfully addresses some essential problems in optimizing the cutting parameters by developing a new geometric-and-physics integrated model of milling and proposing an effective approach to the medial axis transforms of free-form pockets. In this research, an original geometric model of 21/2- and 3-axis CNC milling is developed and integrated with a well-established mechanistic model. A main research contribution is that this integrated model can predict complex milling processes in higher fidelity with instantaneous material remove rates, cutting forces and spindle powers, compared to prior machining models. In the geometric model, an in-process workpiece model is introduced by using a group of discrete Z-layers and applying the B-Rep scheme to represent the workpiece shape on each layer, in order to accurately represent instantaneous cutter-and-workpiece engagement in 2Yz- and 3-axis milling. Hence, the un-deformed chip geometry can be found even for complex part milling, which is then fed to the mechanistic model to predict instantaneous cutting forces. By using this integrated model, cutting parameters can be optimized for profiling, pocketing, and surface milling to ensure steady cut and the maximum material removal rates. This model has been verified by experiments, and will be implemented into a software tool for Bombardier Aerospace. Another important research in this work is to propose aggressive roughing of free-form pockets for ultimately high cutting efficiency. For this purpose, an accurate, efficient approach to the medial axis transforms of free-form pockets and an optimal approach to multiple cutters selection and their path generation are proposed. The main contributions of this research include (1) a new mathematical model of medial axis point, (2) an innovative global optimization solver, the hybrid global optimization method, (3) an optimization model of selecting multiple cutters for the maximum material removal rate. This research can substantially promote aggressive roughing in the machining industry to increase cutting efficiency of free-form pockets. The technique has been validated using considerable number of cutting tests and can be directly implemented into commercial CAD/CAM softwar

On the stability of 2 \sqrt{2} x 2 \sqrt{2} oxygen ordered superstructures in YBa2Cu3O6+x

We have compared the ground-state energy of several observed or proposed " 2 \sqrt{2} x 2 \sqrt{2} oxygen (O) ordered superstructures " (from now on HS), with those of "chain superstructures" (CS) (in which the O atoms of the basal plane are ordered in chains), for different compositions x in YBa2Cu3O6+x. The model Hamiltonian contains i) the Madelung energy, ii) a term linear in the difference between Cu and O hole occupancies which controls charge transfer, and iii) covalency effects based on known results for $t-J$ models in one and two dimensions. The optimum distribution of charge is determined minimizing the total energy, and depends on two parameters which are determined from known results for x=1 and x=0.5. We obtain that on the O lean side, only CS are stable, while for x=7/8, a HS with regularly spaced O vacancies added to the x=1 structure is more stable than the corresponding CS for the same x. We find that the detailed positions of the atoms in the structure, and long-range Coulomb interactions, are crucial for the electronic structure, the mechanism of charge transfer, the stability of the different phases, and the possibility of phase separation.Comment: 24 text pages, Latex, one fig. included as ps file, to be publisheb in Phys. Rev.

Plant-herbivorous beetle networks: molecular characterization of trophic ecology within a threatened steppic environment

DNA barcoding facilitates many evolutionary and ecological studies, including the examination of the dietary diversity of herbivores. In this study, we present a survey of ecological associations between herbivorous beetles and host plants from seriously threatened European steppic grasslands. We determined host plants for the majority (65%) of steppic leaf beetles (55 species) and weevils (59) known from central Europe using two barcodes (trnL and rbcL) and two sequencing strategies (Sanger for mono/oligophagous species and Illumina for polyphagous taxa). To better understand the ecological associations between steppic beetles and their host plants, we tested the hypothesis that leaf beetles and weevils differ in food selection as a result of their phylogenetic relations (within genera and between families) and interactions with host plants. We found 224 links between the beetles and the plants. Beetles belonging to seven genera feed on the same or related plants. Their preferences were probably inherited from common ancestors and/or resulted from the host plant's chemistry. Beetles from four genera feed on different plants, possibly reducing intrageneric competition and possibly due to an adaptation to different plant chemical defences. We found significant correlations between the numbers of leaf beetle and weevil species feeding on particular plants for polyphagous taxa, but not for nonpolyphagous beetles. Finally, we found that the previous identifications of host plants based on direct observations are generally concordant with host plant barcoding from insect gut. Our results expand basic knowledge about the trophic relations of steppic beetles and plants and are immediately useful for conservation purposes

Design of concrete structures using structural optimization based on the stress field method

The Elastic Plastic Stress Field method (EPSF) is a widely used tool for design and verification of structural concrete works. EPSF uses the elastic-plastic material behavior, which allows accounting for compatibility conditions and thus provides exact solutions according to limit analysis. By applying structural optimization method on the basis of EPSF, an automatic approach for the optimal design of reinforced concrete structures is developed. Optimal design is obtained by solving a minimization problem with a number of compliance constraints. The result respects the yield conditions of both materials and the deformation capacity requirements of the structure. The efficiency of the approach is illustrated by a set of examples

Evaluation Technique for The Failure Life Scatter of Lead-Free Solder Joints in Electronic Device

Recently, the electronic device equipment using a semiconductor is widespread to all industrial fields. Solder is used to mount electronic parts, such as resistors and capacitors, on printed-circuit boards in almost all electronic devices. However, since in many cases the thermal expansion coefficients of electronic parts and PCBs are different, cyclic thermal stress and strain causes solder fatigue and device failure. Especially in the power electronic module and car electric module, the evaluation of thermal fatigue life is important. It is understood that the fatigue life of some electronic devices shows large scatter in the thermal cycle test, even if their design is the same. The dispersion of design factors such as shape, size and material properties of solder joints is thought as one of these reasons. Moreover, in the case of chip components, it is thought that the interacting effect by the structural asymmetry due to the unbalance solder joints would influence reliability. At the same time, the changeover from eutectic Sn-Pb solder to lead-free solder has been driven by environmental concerns. Therefore, it cannot be disregard as the main factor of the reliability evaluation in the solder joints. In this study, how the dispersion of design factors and the interacting effect between the design factors influences the fatigue life in lead-free solder joint was investigated by the analytical approach. It is understood that the thermal fatigue life of solder joints can be estimated by the inelastic strain range obtained from the FEM analysis. Recently, it is demanded to evaluate the final failure life of electronic components. So, it is necessary to evaluate not only crack initiation but also crack propagation. In this study, crack propagation analysis was carried out by using a new approach and the failure life was evaluated on the basis of Manson-Coffin's law and Miner's rule. Moreover, sensitivity analyses were carried out to study the main effect of the dispersion of each factor on solder joints. And then, the interacting effect between the factors on the reliability was studied by considering the structural asymmetry due to the unbalanced solder joints. As a result, a practical evaluating technique for the failure life scatter of solder joints was proposed

Genome-wide and gene-specific epigenomic platforms for hepatocellular carcinoma biomarker development trials

The majority of the epigenomic reports in hepatocellular carcinoma have focused on identifying novel differentially methylated drivers or passengers of the oncogenic process. Few reports have considered the technologies in place for clinical translation of newly identified biomarkers. The aim of this study was to identify epigenomic technologies that need only a small number of samples to discriminate HCC from non-HCC tissue, a basic requirement for biomarker development trials. To assess that potential, we used quantitative Methylation Specific PCR, oligonucleotide tiling arrays, and Methylation BeadChip assays. Concurrent global DNA hypomethylation, gene-specific hypermethylation, and chromatin alterations were observed as a hallmark of HCC. A global loss of promoter methylation was observed in HCC with the Illumina BeadChip assays and the Nimblegen oligonucleotide arrays. HCC samples had lower median methylation peak scores and a reduced number of significant promoter-wide methylated probes. Promoter hypermethylation of RASSF1A, SSBP2, and B4GALT1 quantified by qMSP had a sensitivity ranging from 38% to 52%, a specificity of 100%, and an AUC from 0.58 to 0.75. A panel combining these genes with HCC risk factors had a sensitivity of 87%, a specificity of 100%, and an AUC of 0.91

Ratiometric G-quadruplex assay for robust lead detection in food samples

Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safet