Overcoming Barriers to Effective Ecological Education : A Malagasy Case Study

Honko Mangrove Conservation and Education is a Western non-governmental organization based in Madagascar that serves as a source of public science education and caters to diverse audiences. My thesis examines barriers of culture, language and epistemology that may constrain Honko’s ability to conduct effective public education, if they decide to expand their organization. Honko should implement a three-pronged action plan that includes a dynamic epistemological model drawing from Western and Traditional Knowledge Systems and an strategy to actively identify changing audience and stakeholder bases. These two steps plus a shift towards constructivist educational philosophy that encourages reflection and incorporates social-context into science could help Honko attain their educational goals and overcome cultural barriers to ecological and public education

Exploring How Institutionalized Scuba Diving Training and the Diver Experience Influences the Diver’s Conceptualization of the Marine Environment

The purpose of this grounded theory mixed methods study is to understand how new scuba dive students are creating meaning of their relationship to the marine environment and how that might be shaped by dive training and dive experiences. Eighty-one scuba dive students enrolled in a standardized entry-level dive course conducted in two locations, participated in this study. Participating dive students completed a questionnaire, composed of closed- and open-ended questions, before, immediately after, and three months after completing the open water dive portion of the dive training process. The quantitative portion of the questionnaires assesses participant’s Nature Relatedness, place attachment to the marine environment, motivations for diving and perceptions of the dive community in relation to self. The qualitative portion allows individuals to reflect upon their own experiences and relationships with the marine environment in their own words. The results of this study indicate that this community of emerging divers possess: (a) positive Nature Relatedness and marine place-attachment that did not significantly change through the dive training process, (b) sightseeing, exploratory, and adventure-related motivations for diving, (c) perceptions of the dive community that matched their own interests, and (d) differences in how they conceptualized the marine environment throughout the dive training and dive experience. As revealed through their responses, new dive students drew upon social constructs to embed their understanding of the marine environment, transforming it from a physical space to an oceanscape imbued with meaning. I use schema theory to help understand both the shifts and continuities seen in how these dive students constructed the oceanscape over time. The findings of this study can inform the Professional Association of Diving Instructors and other dive certification organizations about how to further develop their learn-to-dive curriculum based on the understanding that the cognitive impact of learning to dive goes beyond attaining a certification card

Explorer At Large: Pilot Implementation in Ohio : Evaluation Report 2019-2020

Explorer At Large (XAL) is an education initiative designed to encourage students and their families to be curious and develop an identity as an explorer. XAL delivers a holistic ecosystem of learning opportunities that model curiosity and exploration skills and consists of the following four primary components: 1) freely distributed videos that invite students to observe exploration; 2) instructional materials (teacher guides, hands-on classroom and outdoor activities) that guide students in practicing exploration; 3) field trips to settings in students’ local communities that empower students to experience exploration; and 4) family moments that encourage parent/caregiver engagement and inspires students to share exploration. This report shares evaluation findings from piloting the XAL instructional materials with K and 3rd grade teachers and students from schools in Ohio. The report is following up on an earlier pilot that focused on schools in the Columbus region

Authentic Research through Collaborative Learning (ARC-Learn): Undergraduate Research Experiences in Data Rich Arctic Science

This report serves the formative evaluation of ARC-Learn. The goal of this document is to support the use of evidence to inform programmatic changes and improvements for year two of the program, during which time Cohort One will complete its second year and Cohort Two will complete its first year of activities

The Science of Sungrazers, Sunskirters, and Other Near-Sun Comets

This review addresses our current understanding of comets that venture close to the Sun, and are hence exposed to much more extreme conditions than comets that are typically studied from Earth. The extreme solar heating and plasma environments that these objects encounter change many aspects of their behaviour, thus yielding valuable information on both the comets themselves that complements other data we have on primitive solar system bodies, as well as on the near-solar environment which they traverse. We propose clear definitions for these comets: We use the term near-Sun comets to encompass all objects that pass sunward of the perihelion distance of planet Mercury (0.307 AU). Sunskirters are defined as objects that pass within 33 solar radii of the Sun’s centre, equal to half of Mercury’s perihelion distance, and the commonly-used phrase sungrazers to be objects that reach perihelion within 3.45 solar radii, i.e. the fluid Roche limit. Finally, comets with orbits that intersect the solar photosphere are termed sundivers. We summarize past studies of these objects, as well as the instruments and facilities used to study them, including space-based platforms that have led to a recent revolution in the quantity and quality of relevant observations. Relevant comet populations are described, including the Kreutz, Marsden, Kracht, and Meyer groups, near-Sun asteroids, and a brief discussion of their origins. The importance of light curves and the clues they provide on cometary composition are emphasized, together with what information has been gleaned about nucleus parameters, including the sizes and masses of objects and their families, and their tensile strengths. The physical processes occurring at these objects are considered in some detail, including the disruption of nuclei, sublimation, and ionisation, and we consider the mass, momentum, and energy loss of comets in the corona and those that venture to lower altitudes. The different components of comae and tails are described, including dust, neutral and ionised gases, their chemical reactions, and their contributions to the near-Sun environment. Comet-solar wind interactions are discussed, including the use of comets as probes of solar wind and coronal conditions in their vicinities. We address the relevance of work on comets near the Sun to similar objects orbiting other stars, and conclude with a discussion of future directions for the field and the planned ground- and space-based facilities that will allow us to address those science topics

Measuring psychological need states in sport: Theoretical considerations and a new measure

© 2019 Elsevier Ltd Objectives: Research guided by Self-determination Theory (Deci & Ryan, 1985; Ryan & Deci, 2017) has repeatedly demonstrated the importance of focusing on both the bright (satisfaction) and dark (frustration) sides of the three basic psychological needs. Recently, researchers have also argued for the utility of assessing a third need state, that of “unfulfillment”. In this paper, we outline an effort to develop and provide initial validity evidence for scores of a new multidimensional and sport-specific measure, the Psychological Need States in Sport-Scale (PNSS-S), to assess the satisfaction, frustration, and unfulfillment of all three needs. Method: In Study 1, we developed 46 candidate items, and tested evidence for the factorial structure of the responses to the newly developed items, internal consistency and discriminant validity of the subscale scores. Following refinement, the replication of the favored model was tested using an independent sample of athletes in Study 2. Evidence for the nomological network of the subscales of the new measure was also demonstrated in Study 2. Results: Factor models incorporating all three need states showed poor fit with the data. However, following post-hoc modifications, a six-factor model assessing the need states of satisfaction and frustration, separately for autonomy, competence, and relatedness, was found to have good fit to the data. After refinement, the 29-item six-factor model was found to demonstrate good fit, good standardized factor loadings, factor correlations in the expected directions, and acceptable estimates of internal consistency in Study 2. Tests of nomological networks showed that the six need states were significantly predicted by contextual autonomy, competence, and relatedness support/thwarts as expected. Autonomy and competence need satisfaction were significantly associated with engagement; and competence and relatedness need satisfaction were significantly associated with positive affect. In addition, autonomy and competence need frustration were significantly associated with exhaustion and all three need frustration states significantly predicted negative affect. Conclusions: A tripartite conceptualization of the need states was not empirically supported. Nevertheless, the PNSS-S makes a unique contribution to the sport literature, as it represents the first sport-specific measure of six distinct, yet, correlated states of the satisfaction and frustration of autonomy, competence, and relatedness needs

Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination.

INTRODUCTION: Plasmodium vivax malaria has a wide geographic distribution and poses challenges to malaria elimination that are likely to be greater than those of P. falciparum. Diagnostic tools for P. vivax infection in non-reference laboratory settings are limited to microscopy and rapid diagnostic tests but these are unreliable at low parasitemia. The development and validation of a high-throughput and sensitive assay for P. vivax is a priority. METHODS: A high-throughput LAMP assay targeting a P. vivax mitochondrial gene and deploying colorimetric detection in a 96-well plate format was developed and evaluated in the laboratory. Diagnostic accuracy was compared against microscopy, antigen detection tests and PCR and validated in samples from malaria patients and community controls in a district hospital setting in Sabah, Malaysia. RESULTS: The high throughput LAMP-P. vivax assay (HtLAMP-Pv) performed with an estimated limit of detection of 1.4 parasites/ μL. Assay primers demonstrated cross-reactivity with P. knowlesi but not with other Plasmodium spp. Field testing of HtLAMP-Pv was conducted using 149 samples from symptomatic malaria patients (64 P. vivax, 17 P. falciparum, 56 P. knowlesi, 7 P. malariae, 1 mixed P. knowlesi/P. vivax, with 4 excluded). When compared against multiplex PCR, HtLAMP-Pv demonstrated a sensitivity for P. vivax of 95% (95% CI 87-99%); 61/64), and specificity of 100% (95% CI 86-100%); 25/25) when P. knowlesi samples were excluded. HtLAMP-Pv testing of 112 samples from asymptomatic community controls, 7 of which had submicroscopic P. vivax infections by PCR, showed a sensitivity of 71% (95% CI 29-96%; 5/7) and specificity of 93% (95% CI87-97%; 98/105). CONCLUSION: This novel HtLAMP-P. vivax assay has the potential to be a useful field applicable molecular diagnostic test for P. vivax infection in elimination settings

Neurogenic inflammation after traumatic brain injury and its potentiation of classical inflammation

Background: The neuroinflammatory response following traumatic brain injury (TBI) is known to be a key secondary injury factor that can drive ongoing neuronal injury. Despite this, treatments that have targeted aspects of the inflammatory pathway have not shown significant efficacy in clinical trials. Main body: We suggest that this may be because classical inflammation only represents part of the story, with activation of neurogenic inflammation potentially one of the key initiating inflammatory events following TBI. Indeed, evidence suggests that the transient receptor potential cation channels (TRP channels), TRPV1 and TRPA1, are polymodal receptors that are activated by a variety of stimuli associated with TBI, including mechanical shear stress, leading to the release of neuropeptides such as substance P (SP). SP augments many aspects of the classical inflammatory response via activation of microglia and astrocytes, degranulation of mast cells, and promoting leukocyte migration. Furthermore, SP may initiate the earliest changes seen in blood-brain barrier (BBB) permeability, namely the increased transcellular transport of plasma proteins via activation of caveolae. This is in line with reports that alterations in transcellular transport are seen first following TBI, prior to decreases in expression of tight-junction proteins such as claudin-5 and occludin. Indeed, the receptor for SP, the tachykinin NK1 receptor, is found in caveolae and its activation following TBI may allow influx of albumin and other plasma proteins which directly augment the inflammatory response by activating astrocytes and microglia. Conclusions: As such, the neurogenic inflammatory response can exacerbate classical inflammation via a positive feedback loop, with classical inflammatory mediators such as bradykinin and prostaglandins then further stimulating TRP receptors. Accordingly, complete inhibition of neuroinflammation following TBI may require the inhibition of both classical and neurogenic inflammatory pathways.Frances Corrigan, Kimberley A. Mander, Anna V. Leonard and Robert Vin

Phenotypic Characterization of EIF2AK4 Mutation Carriers in a Large Cohort of Patients Diagnosed Clinically With Pulmonary Arterial Hypertension.

BACKGROUND: Pulmonary arterial hypertension (PAH) is a rare disease with an emerging genetic basis. Heterozygous mutations in the gene encoding the bone morphogenetic protein receptor type 2 (BMPR2) are the commonest genetic cause of PAH, whereas biallelic mutations in the eukaryotic translation initiation factor 2 alpha kinase 4 gene (EIF2AK4) are described in pulmonary veno-occlusive disease/pulmonary capillary hemangiomatosis. Here, we determine the frequency of these mutations and define the genotype-phenotype characteristics in a large cohort of patients diagnosed clinically with PAH. METHODS: Whole-genome sequencing was performed on DNA from patients with idiopathic and heritable PAH and with pulmonary veno-occlusive disease/pulmonary capillary hemangiomatosis recruited to the National Institute of Health Research BioResource-Rare Diseases study. Heterozygous variants in BMPR2 and biallelic EIF2AK4 variants with a minor allele frequency of <1:10 000 in control data sets and predicted to be deleterious (by combined annotation-dependent depletion, PolyPhen-2, and sorting intolerant from tolerant predictions) were identified as potentially causal. Phenotype data from the time of diagnosis were also captured. RESULTS: Eight hundred sixty-four patients with idiopathic or heritable PAH and 16 with pulmonary veno-occlusive disease/pulmonary capillary hemangiomatosis were recruited. Mutations in BMPR2 were identified in 130 patients (14.8%). Biallelic mutations in EIF2AK4 were identified in 5 patients with a clinical diagnosis of pulmonary veno-occlusive disease/pulmonary capillary hemangiomatosis. Furthermore, 9 patients with a clinical diagnosis of PAH carried biallelic EIF2AK4 mutations. These patients had a reduced transfer coefficient for carbon monoxide (Kco; 33% [interquartile range, 30%-35%] predicted) and younger age at diagnosis (29 years; interquartile range, 23-38 years) and more interlobular septal thickening and mediastinal lymphadenopathy on computed tomography of the chest compared with patients with PAH without EIF2AK4 mutations. However, radiological assessment alone could not accurately identify biallelic EIF2AK4 mutation carriers. Patients with PAH with biallelic EIF2AK4 mutations had a shorter survival. CONCLUSIONS: Biallelic EIF2AK4 mutations are found in patients classified clinically as having idiopathic and heritable PAH. These patients cannot be identified reliably by computed tomography, but a low Kco and a young age at diagnosis suggests the underlying molecular diagnosis. Genetic testing can identify these misclassified patients, allowing appropriate management and early referral for lung transplantation