Identification of salicylic acid-independent responses in an Arabidopsis 3 phosphatidylinositol 4-kinase beta double mutant

International audienceBackground and AimsWe have recently shown that an Arabidopsis thaliana double mutant of type III phosphatidylinositol-4-kinases (PI4Ks), pi4kβ1β2, constitutively accumulated a high level of salicylic acid (SA). By crossing this pi4kβ1β2 double mutant with mutants impaired in SA synthesis (such as sid2 impaired in isochorismate synthase) or transduction, we demonstrated that the high SA level was responsible for the dwarfism phenotype of the double mutant. Here we aimed at distinguishing between the SA-dependent and -independent effects triggered by the deficiency in PI4Kβ1 and PI4Kβ2.MethodsTo achieve this, the sid2pi4kβ1β2 triple mutant was a tool of choice. High-throughput analyses of phytohormones were performed on this mutant together with pi4kβ1β2 and sid2 mutants and wild-type plants. Responses to pathogens, namely Hyaloperonospora arabidopsidis, Pseudomonas syringae and Botrytis cinerea, but as well to the non-host fungus Blumeria graminis, were also determined. Callose accumulation was monitored in response to flagellin. Key ResultsWe show here the prominent role of high SA levels in influencing the concentration of many other tested phytohormones, including abscisic acid and its derivatives, the Aspartate-conjugated form of indole-3-acetic acid and some cytokinins such as cis-zeatin. We show that the increased resistance of pi4kβ1β2 plants to the host pathogens Hyaloperonospora arabidopsidis, Pseudomonas syringae pv. tomato DC3000 and Bothrytis cinerea is dependent on accumulation of high SA level. In contrast, accumulation of callose in pi4kβ1β2 after flagellin treatment was independent of SA. Concerning the response to Blumeria graminis, both callose accumulation and fungal penetration were enhanced in the pi4kβ1β2 double mutant compared to wild-type plants. Both of these processes occurred in a SA-independent manner. ConclusionsOur data extensively illustrate the influence of SA on other phytohormone levels. The sid2pi4kβ1β2 triple mutant allowed to uncover the role of PI4Kβ1/β2 per se, thus showing the importance of these enzymes in plant defence responses

European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS).

The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics briefly described below. The formation of reactive oxygen and nitrogen species (RONS) is an established hallmark of our aerobic environment and metabolism but RONS also act as messengers via redox regulation of essential cellular processes. The fact that many diseases have been found to be associated with oxidative stress established the theory of oxidative stress as a trigger of diseases that can be corrected by antioxidant therapy. However, while experimental studies support this thesis, clinical studies still generate controversial results, due to complex pathophysiology of oxidative stress in humans. For future improvement of antioxidant therapy and better understanding of redox-associated disease progression detailed knowledge on the sources and targets of RONS formation and discrimination of their detrimental or beneficial roles is required. In order to advance this important area of biology and medicine, highly synergistic approaches combining a variety of diverse and contrasting disciplines are needed.The EU-ROS consortium (COST Action BM1203) was supported by the European Cooperation in Science and Technology (COST). The present overview represents the final Action dissemination summarizing the major achievements of COST Action BM1203 (EU-ROS) as well as research news and personal views of its members. Some authors were also supported by COST Actions BM1005 (ENOG) and BM1307 (PROTEOSTASIS), as well as funding from the European Commission FP7 and H2020 programmes, and several national funding agencies

Selected features of population development in Czech border regions during 1961-2001 period

Department of Demography and GeodemographyKatedra demografie a geodemografieFaculty of SciencePřírodovědecká fakult

Průzkum názorů jako součást vnější analýzy pro potřeby personálního marketingu

Práce se zabývá zpracováním a vyhodnocením dotazníkového šetření vnějších analýz personálního marketingu pro Synthesii a.s.Dokončená práce s úspěšnou obhajobo

Perspectives of population development in LAG Pošumaví region

Katedra demografie a geodemografieDepartment of Demography and GeodemographyFaculty of SciencePřírodovědecká fakult

Lipid Signaling in Plant Development and Responses to Environmental Stresses

International audienc