Estudio y edición de una versión sefardí aljamiada del Ma‘asé Yehudit

The proposal of the present contribution is to present the critical edition of the oldest Sephardic version known of the Ma‘asé Yehudit. It was published in Constantinople in 1758 included in the booklet Lúaḥ areź, published by Nisim ben Yoná Aškenaźí. The translator of this story (from Hebrew), Yiŝḥac Valero, says in the cover that he copied it in ladino. Effectively, the translation from the numerous biblical quotations and fragments of prayers follow the style from the translations of the Bible in ladino at that time and in the previous centuries. The story follows the textual tradition of the one content in Ḥemdat yamim (Izmir 1731-1732), but it is far from being literal. So, after considering some clues, we think that this is an eclectic version, because doesn’t trace none of those known until know.El propósito de esta contribución es presentar la edición crítica de la más antigua versión sefardí aljamiada del Ma‘asé Yehudit que conocemos. Apareció en Constantinopla en 1758 incluida en el libro Lúaḥ areź, publicada por Nisim ben Yoná Aškenaźí. El traductor de esta historia (del hebreo), Yiŝḥac Valero, dice en la portada que la copió en ladino. Efectivamente, la traducción de las numerosas citas bíblicas y de fragmentos de plegarias, lo son al estilo de los ladinamientos aljamiados en boga en la época y en los siglos precedentes. El relato sigue la tradición textual del contenido en la obra Ḥemdat yamim (Esmirna 1731-1732), pero no es literal, lo que después de algunos indicios nos lleva a pensar que se trata de una versión ecléctica, ya que no calca ninguna de las hasta ahora conocidas

Structural Insights into Viral Determinants of Nematode Mediated Grapevine fanleaf virus Transmission

Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat protein subunits (CP), carries the determinants of this specificity. Here, we provide novel insight into GFLV transmission by nematodes through a comparative structural and functional analysis of two GFLV variants. We isolated a mutant GFLV strain (GFLV-TD) poorly transmissible by nematodes, and showed that the transmission defect is due to a glycine to aspartate mutation at position 297 (Gly297Asp) in the CP. We next determined the crystal structures of the wild-type GFLV strain F13 at 3.0 Å and of GFLV-TD at 2.7 Å resolution. The Gly297Asp mutation mapped to an exposed loop at the outer surface of the capsid and did not affect the conformation of the assembled capsid, nor of individual CP molecules. The loop is part of a positively charged pocket that includes a previously identified determinant of transmission. We propose that this pocket is a ligand-binding site with essential function in GFLV transmission by X. index. Our data suggest that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency. These data provide a first structural insight into the interactions between a plant virus and a nematode vector