759 research outputs found
A concerted systems biology analysis of phenol metabolism in Rhodococcus opacus PD630
Rhodococcus opacus PD630 metabolizes aromatic substrates and naturally produces branched-chain lipids, which are advantageous traits for lignin valorization. To provide insights into its lignocellulose hydrolysate utilization, we performed 13C-pathway tracing, 13C-pulse-tracing, transcriptional profiling, biomass composition analysis, and metabolite profiling in conjunction with 13C-metabolic flux analysis (13C-MFA) of phenol metabolism. We found that 1) phenol is metabolized mainly through the ortho–cleavage pathway; 2) phenol utilization requires a highly active TCA cycle; 3) NADPH is generated mainly via NADPH-dependent isocitrate dehydrogenase; 4) active cataplerotic fluxes increase plasticity in the TCA cycle; and 5) gluconeogenesis occurs partially through the reversed Entner–Doudoroff pathway (EDP). We also found that phenol-fed R. opacus PD630 generally has lower sugar phosphate concentrations (e.g., fructose 1,6-bisphosphatase) compared to metabolite pools in 13C-glucose-fed Escherichia coli (set as internal standards), while its TCA metabolites (e.g., malate, succinate, and α-ketoglutarate) accumulate intracellularly with measurable succinate secretion. In addition, we found that phenol utilization was inhibited by benzoate, while catabolite repressions by other tested carbon substrates (e.g., glucose and acetate) were absent in R. opacus PD630. Three adaptively-evolved strains display very different growth rates when fed with phenol as a sole carbon source, but they maintain a conserved flux network. These findings improve our understanding of R. opacus’ metabolism for future lignin valorization
Hartree-Fock Theory of Skyrmions in Quantum Hall Ferromagnets
We report on a study of the charged-skyrmion or spin-texture excitations
which occur in quantum Hall ferromagnets near odd Landau level filling factors.
Particle-hole symmetry is used to relate the spin-quantum numbers of charged
particle and hole excitations and neutral particle-hole pair excitations.
Hartree-Fock theory is used to provide quantitative estimates of the energies
of these excitations and their dependence on Zeeman coupling strength, Landau
level quantum numbers, and the thicknesses of the two-dimensional electron
layers. For the case of near three we suggest the possibility of first
order phase transitions with increasing Zeeman coupling strength from a many
skyrmion state to one with many maximally spin-polarized quasiparticles.Comment: 26 pages, 10 figure
Structural Invariance of Sunspot Umbrae Over the Solar Cycle: 1993-2004
Measurements of maximum magnetic flux, minimum intensity, and size are
presented for 12 967 sunspot umbrae detected on the NASA/NSO
spectromagnetograms between 1993 and 2004 to study umbral structure and
strength during the solar cycle. The umbrae are selected using an automated
thresholding technique. Measured umbral intensities are first corrected for a
confirming observation of umbral limb-darkening. Log-normal fits to the
observed size distribution confirm that the size spectrum shape does not vary
with time. The intensity-magnetic flux relationship is found to be steady over
the solar cycle. The dependence of umbral size on the magnetic flux and minimum
intensity are also independent of cycle phase and give linear and quadratic
relations, respectively. While the large sample size does show a low amplitude
oscillation in the mean minimum intensity and maximum magnetic flux correlated
with the solar cycle, this can be explained in terms of variations in the mean
umbral size. These size variations, however, are small and do not substantiate
a meaningful change in the size spectrum of the umbrae generated by the Sun.
Thus, in contrast to previous reports, the observations suggest the equilibrium
structure, as testified by the invariant size-magnetic field relationship, as
well as the mean size (i.e. strength) of sunspot umbrae do not significantly
depend on solar cycle phase.Comment: 17 pages, 6 figures. Published in Solar Physic
Spontaneous Coherence and Collective Modes in Double-Layer Quantum Dot Systems
We study the ground state and the collective excitations of
parabolically-confined double-layer quantum dot systems in a strong magnetic
field. We identify parameter regimes where electrons form maximum density
droplet states, quantum-dot analogs of the incompressible states of the bulk
integer quantum Hall effect. In these regimes the Hartree-Fock approximation
and the time-dependent Hartree-Fock approximations can be used to describe the
ground state and collective excitations respectively. We comment on the
relationship between edge excitations of dots and edge magneto-plasmon
excitations of bulk double-layer systems.Comment: 20 pages (figures included) and also available at
http://fangio.magnet.fsu.edu/~jhu/Paper/qdot_cond.ps, replaced to fix figure
Collision statistics of driven granular materials
We present an experimental investigation of the statistical properties of
spherical granular particles on an inclined plane that are excited by an
oscillating side-wall. The data is obtained by high-speed imaging and particle
tracking techniques. We identify all particles in the system and link their
positions to form trajectories over long times. Thus, we identify particle
collisions to measure the effective coefficient of restitution and find a broad
distribution of values for the same impact angles. We find that the energy
inelasticity can take on values greater than one, which implies that the
rotational degrees play an important role in energy transfer. We also measure
the distance and the time between collision events in order to directly
determine the distribution of path lengths and the free times. These
distributions are shown to deviate from expected theoretical forms for elastic
spheres, demonstrating the inherent clustering in this system. We describe the
data with a two-parameter fitting function and use it to calculated the mean
free path and collision time. We find that the ratio of these values is
consistent with the average velocity. The velocity distribution are observed to
be strongly non-Gaussian and do not demonstrate any apparent universal
behavior. We report the scaling of the second moment, which corresponds to the
granular temperature, and higher order moments as a function of distance from
the driving wall. Additionally, we measure long time correlation functions in
both space and in the velocities to probe diffusion in a dissipative gas.Comment: 12 pages, 4 figures, uses revtex
Non-monotonic variation with salt concentration of the second virial coefficient in protein solutions
The osmotic virial coefficient of globular protein solutions is
calculated as a function of added salt concentration at fixed pH by computer
simulations of the ``primitive model''. The salt and counter-ions as well as a
discrete charge pattern on the protein surface are explicitly incorporated. For
parameters roughly corresponding to lysozyme, we find that first
decreases with added salt concentration up to a threshold concentration, then
increases to a maximum, and then decreases again upon further raising the ionic
strength. Our studies demonstrate that the existence of a discrete charge
pattern on the protein surface profoundly influences the effective interactions
and that non-linear Poisson Boltzmann and Derjaguin-Landau-Verwey-Overbeek
(DLVO) theory fail for large ionic strength. The observed non-monotonicity of
is compared to experiments. Implications for protein crystallization are
discussed.Comment: 43 pages, including 17 figure
Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction
Since both myocardium and vasculature in the heart are excessively damaged following myocardial infarction (MI), therapeutic strategies for treating MI hearts should concurrently target both so as to achieve true cardiac repair. Here we demonstrate a concomitant method that exploits the advantages of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) and human mesenchymal stem cell-loaded patch (hMSC-PA) to amplify cardiac repair in a rat MI model. Epicardially implanted hMSC-PA provide a complimentary microenvironment which enhances vascular regeneration through prolonged secretion of paracrine factors, but more importantly it significantly improves the retention and engraftment of intramyocardially injected hiPSC-CMs which ultimately restore the cardiac function. Notably, the majority of injected hiPSC-CMs display adult CMs like morphology suggesting that the secretomic milieu of hMSC-PA constitutes pleiotropic effects in vivo. We provide compelling evidence that this dual approach can be a promising means to enhance cardiac repair on MI hearts.11Ysciescopu
Six Years of Chandra Observations of Supernova Remnants
We present a review of the first six years of Chandra X-ray Observatory
observations of supernova remnants. From the official "first-light" observation
of Cassiopeia A that revealed for the first time the compact remnant of the
explosion, to the recent million-second spectrally-resolved observation that
revealed new details of the stellar composition and dynamics of the original
explosion, Chandra observations have provided new insights into the supernova
phenomenon. We present an admittedly biased overview of six years of these
observations, highlighting new discoveries made possible by Chandra's unique
capabilities.Comment: 82 pages, 28 figures, for the book Astrophysics Update
Modeling the Subsurface Structure of Sunspots
While sunspots are easily observed at the solar surface, determining their
subsurface structure is not trivial. There are two main hypotheses for the
subsurface structure of sunspots: the monolithic model and the cluster model.
Local helioseismology is the only means by which we can investigate
subphotospheric structure. However, as current linear inversion techniques do
not yet allow helioseismology to probe the internal structure with sufficient
confidence to distinguish between the monolith and cluster models, the
development of physically realistic sunspot models are a priority for
helioseismologists. This is because they are not only important indicators of
the variety of physical effects that may influence helioseismic inferences in
active regions, but they also enable detailed assessments of the validity of
helioseismic interpretations through numerical forward modeling. In this paper,
we provide a critical review of the existing sunspot models and an overview of
numerical methods employed to model wave propagation through model sunspots. We
then carry out an helioseismic analysis of the sunspot in Active Region 9787
and address the serious inconsistencies uncovered by
\citeauthor{gizonetal2009}~(\citeyear{gizonetal2009,gizonetal2009a}). We find
that this sunspot is most probably associated with a shallow, positive
wave-speed perturbation (unlike the traditional two-layer model) and that
travel-time measurements are consistent with a horizontal outflow in the
surrounding moat.Comment: 73 pages, 19 figures, accepted by Solar Physic
Anisotropic flow of charged hadrons, pions and (anti-)protons measured at high transverse momentum in Pb-Pb collisions at TeV
The elliptic, , triangular, , and quadrangular, , azimuthal
anisotropic flow coefficients are measured for unidentified charged particles,
pions and (anti-)protons in Pb-Pb collisions at TeV
with the ALICE detector at the Large Hadron Collider. Results obtained with the
event plane and four-particle cumulant methods are reported for the
pseudo-rapidity range at different collision centralities and as a
function of transverse momentum, , out to GeV/.
The observed non-zero elliptic and triangular flow depends only weakly on
transverse momentum for GeV/. The small dependence
of the difference between elliptic flow results obtained from the event plane
and four-particle cumulant methods suggests a common origin of flow
fluctuations up to GeV/. The magnitude of the (anti-)proton
elliptic and triangular flow is larger than that of pions out to at least
GeV/ indicating that the particle type dependence persists out
to high .Comment: 16 pages, 5 captioned figures, authors from page 11, published
version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/186
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