Proyecto de Variante de la carretera CV-575 entre las carreteras CV-573 y CV-600. Término municipal de Lloc Nou d'En Fenollet (Valencia)

[EN] The main purpose of this project is to define all the necessary works for the Alternative CV-575 road between the CV-573 and CV-600 road which aims to solve the current problems crossing the CV-575 road within the population of Lloc Nou d'En Fenollet. It is planned at the intersection of highways 575 and cv-cv-573 roundabout of 52 m. outside diameter and its branches are defined. The roundabout at the end of the variant at its intersection with the CV-600 is executed The current structure on the river Barxeta, verifying the functionality of it at the risk of avenues fail. The type cv-575 road section is 7 m carriageway, hard shoulder of 1.5 m 0.50 m berms, projected path speed is 80 km / h. The final section is the type 3221, consists of 14 cm of bituminous hot mix and 35 cm artificial gravel. A transverse drainage projects in the branches of the arbor run by two-cell reinforced concrete frame 2x2x1.8 m and 31 m in length. The length of the variant is 784.615 m, the execution time of the works of 7 months, a total of 25,327 m² of expropriation of private plots and budget execution need material amounts to ' 984,877.26[ES] El objeto fundamental del presente proyecto es definir todas las obras necesarias para la Variante de la carretera CV-575 entre las carreteras CV-573 y CV-600, la cual pretende solventar los problemas que actualmente presenta la travesía de la carretera CV-575 por dentro de la población de Lloc Nou d'En Fenollet. Se proyecta en la intersección de las carreteras cv-575 y cv-573 una glorieta de 52 m. de diámetro exterior y se definen sus ramales. La glorieta al final de la variante en su intersección con la CV-600 se encuentra ejecutada Se aprovecha la actual estructura sobre el río Barxeta, comprobándose a la funcionalidad de la misma ante el riesgo de avenidas. La sección tipo de la carretera cv-575 es de 7 m de calzada, arcenes de 1,5 m y bermas de 0,50 m, la velocidad del trazado proyectado es de 80 km/h. La Sección de firme es del tipo 3221, formada por 14 cm de mezcla bituminosa en caliente y 35 cm de zahorra artificial. Se proyecta un drenaje transversal en un los ramales de la glorieta a ejecutar mediante marco bicelular de hormigón armado de 2x2x1.8 m y 31 m de longitud. La longitud de la variante es de 784,615 m, el plazo de ejecución de las obras de 7 meses, se necesitan un total de expropiación de 25.327 m² de parcelas privadas y el presupuesto ejecución material asciende a 984.877,26 '[CA] L'objecte fonamental del present projecte és definir totes les obres necessàries per a la Variant de la carretera CV-575 entre les carreteres CV-573 i CV-600, la qual pretén resoldre els problemes que actualment presenta la travessia de la carretera CV-575 per dins de la població de Lloc Nou d'En Fenollet. Es projecta en la intersecció de les carreteres cv-575 i cv-573 una glorieta de 52 m. de diàmetre exterior i es definixen els seus ramals. La glorieta al final de la variant en la seua intersecció amb la CV-600 es troba executada S'aprofita l'actual estructura sobre el riu Barxeta, comprovant-se a la funcionalitat del mateix ant el risc d'avingudes. La secció tipus de la carretera cv-575 és de 7 m de calçada, voreres d'emergència de 1,5 m'i bermes de 0,50 m, la velocitat del traçat projectat és de 80 km/h. La Secció de ferm és del tipus 3221, formada per 14 cm de mescla bituminosa en calent i 35 cm de llast artificial. Es projecta un drenatge transversal en un els ramals de la glorieta a executar per mitjà de marc bicel'lular de formigó armat de 2x2x1.8 m'i 31 m de longitud. La longitud de la variant és de 784,615 m, el termini d'execució de les obres de 7 mesos, es necessiten un total d'expropiació de 25.327 m² de parcel'les privades i el pressupost execució material ascendix a 984.877,26 EURRosell Esteve, MA. (2014). Proyecto de Variante de la carretera CV-575 entre las carreteras CV-573 y CV-600. Término municipal de Lloc Nou d'En Fenollet (Valencia). http://hdl.handle.net/10251/40302.Archivo delegad

Proyecto de instalación de riego localizado y fertilización en la finca rústica "Azagador" en el TM de Enguera (Valencia)

[ES] En las últimas décadas, conscientes de la existencia del agua como un bien escaso, con previsiones de demanda de agua y alimentos cada vez mayores, desde la administración se ha potenciado el uso de sistemas de producción donde el uso de recursos se realice de forma más racional. La política de modernización de regadios, se enmarca dentro de esta nueva visión de un mejor aprovechamiento de los recursos naturales, sustituyendo regadíos tradicionales de riego por inundación o riego “a manta”, por otros sistemas de riego más eficaces y eficientes, como el riego por aspersión, o el riego localizado o riego por goteo. El proyecto que se presenta, es un proyecto de instalación de riego localizado y fertilización en la finca rústica denominada “Azagador” en el T.M. de Enguera (Valencia). En este municipio de la provincia de Valencia, en los últimos años se han llevado a cabo obras de mejora en las zonas de regadio tradicional, pero también, se ha creado la infraestructura necesaria para poner en regadio zonas de secano con cultivo de olivo principalmente y con rendimientos aceptables en años no demasiado secos. El proyecto de instalación de riego localizado en la finca “Azagador” estudia y valora las necesidades de agua y fertilizantes del cultivo de olivo existente, y los medios necesarios que le permitan obtener una producción óptima con el mayor respeto hacia el medio ambiente.[EN] In the last decades, the demand forecasts of water and food are growing up. Aware of it, and thinking about water as a limited source, the governments have encouraged to use production systems where we can use resources rationality. The policy of modernizing irrigation, claims to change traditional systems that waste a lot of water for other more effective and efficient, like the sprinkler irrigation, or the trickle one. This is a project of trickle or drip irrigation in a farming land named “Azagador” with a crop of olives trees in the town of Enguera, Valencia. In the last years, improvements have been made in crops with traditional irrigation systems. Moreover, infrastructures to make easy this kind of irrigation have been created in crop fields where we can get an enough production in years not too dry. The project studies and values the needs for water and fertilizers at this crop field, and also the needed environmentally-friendly ways to get an optimal production.Cerda Esteve, MA. (2015). Proyecto de instalación de riego localizado y fertilización en la finca rústica "Azagador" en el TM de Enguera (Valencia). http://hdl.handle.net/10251/55657.TFG

How effective are biodiversity conservation payments in Mexico?

We assess the additional forest cover protected by 13 rural communities located in the southern state of Chiapas, Mexico, as a result of the economic incentives received through the country's national program of payments for biodiversity conservation. We use spatially explicit data at the intra-community level to define a credible counterfactual of conservation outcomes.We use covariate-matching specifications associated with spatially explicit variables and difference-in-difference estimators to determine the treatment effect. We estimate that the additional conservation represents between 12 and 14.7 percent of forest area enrolled in the program in comparison to control areas. Despite this high degree of additionality, we also observe lack of compliance in some plots participating in the PES program. This lack of compliance casts doubt on the ability of payments alone to guarantee long-term additionality in context of high deforestation rates, even with an augmented program budget or extension of participation to communities not yet enrolled. (Résumé d'auteur

Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan

[EN] Chitosan is a natural polymer with applications in agriculture, which causes plasma membrane permeabilisation and induction of intracellular reactive oxygen species (ROS) in plants. Chitosan has been mostly applied in the phylloplane to control plant diseases and to enhance plant defences, but has also been considered for controlling root pests. However, the effect of chitosan on roots is virtually unknown. In this work, we show that chitosan interfered with auxin homeostasis in Arabidopsis roots, promoting a 2-3 fold accumulation of indole acetic acid (IAA). We observed chitosan dose-dependent alterations of auxin synthesis, transport and signalling in Arabidopsis roots. As a consequence, high doses of chitosan reduce WOX5 expression in the root apical meristem and arrest root growth. Chitosan also propitiates accumulation of salicylic (SA) and jasmonic (JA) acids in Arabidopsis roots by induction of genes involved in their biosynthesis and signalling. In addition, high-dose chitosan irrigation of tomato and barley plants also arrests root development. Tomato root apices treated with chitosan showed isodiametric cells respect to rectangular cells in the controls. We found that chitosan causes strong alterations in root cell morphology. Our results highlight the importance of considering chitosan dose during agronomical applications to the rhizosphere.This work was supported by AGL 2015 66833-R Grant from the Spanish Ministry of Economy and Competitiveness Grant AGL 2015. We would like to thank Drs Isabel Lopez-Diaz and Esther Carrera for plant hormone quantitation (IBMCP, Valencia, Spain). Part of this work was filed for a patent (P201431399) by L. V. Lopez-Llorca, F. Lopez-Moya and N. Escudero as inventors. We would like to thank Dr Michael Kershaw (University of Exeter) for his English revision and critical comments of the manuscript. We also thank Ms Marta Suarez-Fernandez (University of Alicante) and Mr Alfonso Prieto for their technical support. All the authors reviewed and approved the manuscript.Lopez-Moya, F.; Escudero, N.; Zavala-Gonzalez, EA.; Esteve-Bruna, D.; Blazquez Rodriguez, MA.; Alabadí Diego, D.; Lopez-Llorca, LV. (2017). Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan. Scientific Reports. 7:1-14. https://doi.org/10.1038/s41598-017-16874-5S1147Savary, S., Ficke, A., Aubertot, J.-N. & Hollier, C. Crop losses due to diseases and their implications for global food production losses and food security. Food Sec. 4(4), 519–37 (2012).Pimentel, D., Zuniga, R. & Morrison, D. Update on the environmental and economic costs associated with alien-invasive species in the United States. Ecol. Econ. 52(3), 273–88 (2005).El-Hadrami, A. & Adam, L. R. Hadrami El, I. & Daayf, F. Chitosan in plant protection. Mar. Drugs. 8(4), 968–987 (2010).Kumar, R. M. N. V. A review of chitin and chitosan applications. React. Funct. Poly. 46(1), 1–27 (2000).Mayakrishnan, V., Kannappan, P., Abdullah, N. & Ali, A. A. B. Cardioprotective activity of polysaccharides derived from marine algae: an overview. Trends Food Sci. Technol. 30, 98–104 (2013).Lopez-Moya, F. et al. Carbon and nitrogen limitation increase chitosan antifungal activity in Neurospora crassa and fungal human pathogens. Fun. Biol. 119(2-3), 154–69 (2015).Benhamou, N. & Theriault, G. Treatment with chitosan enhances resistance of tomato plants to the crown and root rot pathogen Fusarium oxysporum f. sp. radicis-lycopersici. Physiol Mol Plant Path. 44(1), 33–52 (1992).Ohta, K., Taniguchi, A., Konishi, N. & Hosoki, T. Chitosan treatment affects plant growth and flower quality in Eustoma grandiflorum. HortScience. 34(2), 233–234 (1999).Van, S. N., Minh, H. D. & Anh, D. N. Study on chitosan nanoparticles on biophysical characteristics and growth of Robusta coffee in green house. Biocatal Agric Biotechnol. 2(4), 289–294 (2013).Kananont, N., Pichvangkura, R., Chanprame, S., Chadchawan, S. & Limpanavech, P. Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Sci. Hortic. 124(2), 239–247 (2010).Malerba, M. & Cerana, R. Chitosan Effects on Plant Systems. Int. J. Mol. Sci. 17(7), pii: E996 (2016).Uthairatanakij, A., da Silva, J. A. T. & Obsuwan, K. Chitosan for improving orchid production and quality. Orchid Sci. Biotechnol. 1(1), 1–5 (2007).Limpanavech, P. et al. Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Sci. Hortic. 116(1), 65–72 (2008).Nge, K. L., New, N., Chandrkrachang, S. & Stevens, W. F. Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 170(6), 1185–90 (2006).Khan, T. A., Peh, K. K. & Ch’ng, H. S. Reporting degree of deacetylation values of chitosan: the influence of analytical methods. J. Pharma. Sci. 5(3), 205–12 (2002).Iriti, M. & Faoro, F. Bioactivity of grape chemicals for human health. Nat. Prod. Commun. 4(5), 611–34 (2009).Pitta-Alvarez, S. I. & Giulietti, A. M. Influence of chitosan, acetic acid and citric acid on growth and tropane alkaloid production in transformed roots of Brugmansia candida effect of medium pH and growth phase. Plant Cell Tissue Organ Cult. 59(1), 31–38 (1999).Sivanandhan, G. et al. Chitosan enhances with anolides production in adventitious root cultures of Withania somnifera (L.) Dunal. Ind. Crops Prod. 37, 124–129 (2012).Chatelain, P. G., Pintado, M. E. & Vasconcelos, M. W. Evaluation of chitooligosaccharide application on mineral accumulation and plant growth in Phaseolus vulgaris. Plant Sci. 214(15), 134–40 (2014).El-Tantawi, E. M. Behaviour of tomato plants as affected by spraying with chitosan and aminofort as natural stimulator substances under application of soil organic amendments. Pak. J. Biol. Sci. 12(17), 1164–73 (2009).Sharp, R. G. 2013. A review of the applications of chitin and its derivatives in agriculture to modify plant-microbial interactions and improve crop yields. Agronomy. 3, 757–793 (2013).Aranega-Bou, P., de la O Leyva, M., Finiti, I., García-Agustín, P. & González-Bosch, C. Priming of plant resistance by natural compounds. Hexanoic acid as a model. Front. Plant Sci. 1(5), 488 (2014).Kwak, J. M., Nguyen, V. & Schroeder, J. I. The role of reactive oxygen species in hormonal responses. Plant Physiol. 141(2), 323–9 (2006).Lopez-Moya, F. & Lopez-Llorca, L. V. Omics for investigating chitosan as an antifungal and gene modulator. J. Fungi. 2(1), 1–11 (2016).Issak, M. et al. Neither endogenous abscisic acid nor endogenous jasmonate is involved in salicylic acid-, yeast elicitor-, orchitosan-induced stomatal closure in Arabidopsis thaliana. Biosc. Biotechnol. Biochem. 77(5), 1111–3 (2013).Vidhyasekaran, P. Switching on Plant Innate Immunity Signaling Systems: Bioengineering and Molecular Manipulation Of PAMP PIMP PRR Signaling Complex. Signaling and Communication In Plants. Chapt. 3. Switching on Plant Immune Signaling Systems using Microbe-Associated Molecular Patterns/section 3.6 Manipulation of Plant Immune System using Chitosan 144–148, (Springer, 2016).Baque, Md. A., Shiragi, Md. H. K., Lee, E. J. & Paek, K.-Y. Elicitor effect of chitosan and pectin on the biosynthesis of anthraquinones, phenolics and flavonoids in adventitious root suspension cultures of Morinda citrifolia (L.). Aust. J. Crop Sci. 6(9), 1349–1355 (2012).Khalil, M. S. & Badawy, M. E. I. Nematicidal activity of a biopolymer chitosan at different molecular weights against root-knot nematode. Meloidogyne incognita. Plant Prot. Sci. 48(4), 170–178 (2012).Sarkar, A. K. et al. Conserved factors regulate signalling in Arabidopsis thaliana shoot and root stem cell organizers. Nature. 446(7137), 811–4 (2007).Ding, Z. & Friml, J. Auxin regulates distal stem cell differentiation in Arabidopsis roots. Proc. Natl. Acad. Sci. USA 107(26), 12046–12051 (2010).Tian, H. et al. WOX5-IAA17 feedback circuit-mediated cellular auxin response is crucial for the patterning of root stem cell niches in Arabidopsis. Mol. Plant. 7(2), 277–89 (2014).Tiwari, S. B., Hagen, G. & Guilfoyle, T. The roles of auxin response factor domains in auxin-responsive transcription. Plant Cell. 15(2), 533–43 (2003).Doares, S. H., Syrovets, T., Weiler, E. W. & Ryan, C. A. Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway. Proc. Natl. Acad. Sci. USA 92(10), 4095–4098 (1995).Chandra, S. et al. Chitosan nanoparticles: A positive modulator of innate immune responses in plants. Sci. Rep. 5, 15195 (2015).Escudero, N. et al. Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia. Fun. Biol. 120(4), 572–85 (2016).Escudero, N. et al. Chitosan Increases Tomato Root Colonization by Pochonia chlamydosporia and Their Combination Reduces Root-Knot Nematode Damage. Front. Plant Sci. 8, 1415 (2017).Atkinson, N. J., Lilley, C. J. & Urwin, P. E. Identification of Genes Involved in the Response of Arabidopsis to Simultaneous Biotic and Abiotic Stresses. Plant Physiol. 162(4), 2028–2041 (2013).Kazan, K. Auxin and the integration of environmental signals into plant root development. Ann Bot 112(9), 1655–1665 (2013).Overvoorde, P., Fukaki, H. & Beeckman, T. Auxin control of root development. Cold Spring Harb. Perspect. Biol. 2(6), a001537 (2010).Petricka, J. J., Winter, C. M. & Benfey, P. N. Control of Arabidopsis Root Development. Ann. Rev. Plant Biol. 63, 563–590 (2012).Iriti, M. & Faoro, F. Chitosan as a MAMP, searching for a PRR. Plant Signal Behav. 4(1), 66–68 (2009).Jones, A. M. A new look at stress: abscisic acid patterns and dynamics at high-resolution. New Phytol. 210(1), 38–44 (2015).Wasternack, C. Jasmonates: An Update on Biosynthesis, Signal Transduction and Action in Plant Stress Response, Growth and Development. Ann. Bot. 100(4), 681–697 (2007).Wasternack, C. & Hause, B. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. Ann. Bot. 111(6), 1021–1058 (2013).Kazan, K. Diverse roles of jasmonates and ethylene in abiotic stress tolerance. Trends Plant Sci. 20(4), 219–29 (2015).Ning, Y., Liu, W. & Wang, G. L. Balancing Immunity and Yield in Crop Plants. Trends Plant Sci., 1385(17), 30206–6 (2017). pii: S1360.Zhang, R. Q., Zhu, H. H., Zhao, H. Q. & Yao, Q. Arbuscular mycorrhizal fungal inoculation increases phenolic synthesis in clover roots via hydrogen peroxide, salicylic acid and nitric oxide signaling pathways. J. Plant Physiol. 170(1), 74–9 (2013).Dar, T. A., Uddin, M., Masroor, A. M., Hakeem, K. R. & Jaleel, H. 2015. Jasmonates counter plant stress: A Review. Environ. Exp. Bot. 115, 49–57 (2015).Kazan, K. & Lyons, R. 2016. The link between flowering time and stress tolerance. J Exp Bot. 67(1), 47–60 (2016).Jeong, S. et al. Rootin, a compound that inhibits root development through modulating PIN-mediated auxin distribution. Plant Sci. 233, 116–26 (2015).Amborabé, B. E., Bonmort, J., Fleurat-Lessard, P. & Roblin, G. Early events induced by chitosan on plant cells. J. Exp. Bot. 59(9), 2317–2324 (2008).Chen, X. Y. & Kim, J. Y. Callose synthesis in higher plants. Plant Signal Behav. 4(6), 489–492 (2009).Vasil’ev, L. A. et al. Chitosan-induced programmed cell death in plants. Biochem (Mosc). 74(9), 1035–43 (2009).Friml, J. et al. Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis. Nature. 426(6963), 147–153 (2003).Xu, J. et al. A molecular framework for plant regeneration. Science. 311(5759), 385–8 (2006).Cheng, Y., Dai, X. & Zhao, Y. Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. Genes Dev. 20(13), 1790–1799 (2006).Cao, H., Bowling, S. A., Gordon, A. S. & Dong, X. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of Systemic Acquired Resistance. Plant Cell. 6(11), 1583–92 (1994).Dobón, A., Wulff, B. B., Canet, J. V., Fort, P. & Tornero, P. An allele of Arabidopsis COI1 with hypo- and hypermorphic phenotypes in plant growth, defence and fertility. Plos One. 8(1), e55115 (2013).Fernández-Calvo, P. et al. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. Plant Cell. 23(2), 701–15 (2011).Ripoll, J. J., Ferrándiz, C., Martínez-Laborda, A. & Vera, A. PEPPER, a novel K-homology domain gene, regulates vegetative and gynoecium development in Arabidopsis. Dev. Biol. 289(2), 346–59 (2006).Bordallo, J. J. et al. Colonization of plant roots by egg-parasitic and nematode-trapping fungi. New Phytol. 154(2), 491–499 (2002).Palma-Guerrero, J., Jansson, H. B., Salinas, J. & Lopez-Llorca, L. V. Effect of chitosan on hyphal growth and spore germination of plant pathogenic and biocontrol fungi. J. App. Microbiol. 104(2), 541–53 (2008).Seo, M., Jikumaru, Y. & Kamiya, Y. Profiling of hormones and related metabolites in seed dormancy and germination studies. Methods Mol. Biol. 773, 99–111 (2011).Sabatini, S. et al. An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root. Cell. 99, 463–472 (1999).Weigel, D. & Glazebrook, J. Arabidopsis: A Laboratory Manual. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2002).Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25, 402–408 (2001).Escudero, N. & Lopez-Llorca, L. V. Effects on plant growth and root-knot nematode infection of an endophytic GFP transformant of the nematophagous fungus Pochonia chlamydosporia. Symbiosis. 57(1), 33–42 (2012).Maciá-Vicente, J. G., Jansson, H. B., Talbot, N. J. & Lopez-Llorca, L. V. Real-time PCR quantification and live-cell imaging of endophytic colonization of barley (Hordeum ytvulgare) roots by Fusarium equiseti and Pochonia chlamydosporia. New Phytolo. 182(1), 213–28 (2009).Underwood, A. J. Experiments in ecology: their logical design and interpretation using analysis of variance. (Cambridge University Press, Cambridge, 1997).Bartlett, M. S. 1937. Properties of sufficiency and statistical tests. Proc. Royal. Soci. London Math. 160, 268–282 (1937)

Machine Learning-Based Integration of Prognostic Magnetic Resonance Imaging Biomarkers for Myometrial Invasion Stratification in Endometrial Cancer

[EN] Background: Estimation of the depth of myometrial invasion (MI) in endometrial cancer is pivotal in the preoperatively staging. Magnetic resonance (MR) reports suffer from human subjectivity. Multiparametric MR imaging radiomics and parameters may improve the diagnostic accuracy. Purpose: To discriminate between patients with MI ¿ 50% using a machine learning-based model combining texture features and descriptors from preoperatively MR images. Study Type: Retrospective. Population: One hundred forty-three women with endometrial cancer were included. The series was split into training (n = 107, 46 with MI ¿ 50%) and test (n = 36, 16 with MI ¿ 50%) cohorts. Field Strength/Sequences: Fast spin echo T2-weighted (T2W), diffusion-weighted (DW), and T1-weighted gradient echo dynamic contrast-enhanced (DCE) sequences were obtained at 1.5 or 3 T magnets. Assessment: Tumors were manually segmented slice-by-slice. Texture metrics were calculated from T2W and ADC map images. Also, the apparent diffusion coefficient (ADC), wash-in slope, wash-out slope, initial area under the curve at 60 sec and at 90 sec, initial slope, time to peak and peak amplitude maps from DCE sequences were obtained as parameters. MR diagnostic models using single-sequence features and a combination of features and parameters from the three sequences were built to estimate MI using Adaboost methods. The pathological depth of MI was used as gold standard. Statistical Test: Area under the receiver operating characteristic curve (AUROC), sensitivity, specificity, accuracy, positive predictive value, negative predictive value, precision and recall were computed to assess the Adaboost models performance. Results: The diagnostic model based on the features and parameters combination showed the best performance to depict patient with MI ¿ 50% in the test cohort (accuracy = 86.1% and AUROC = 87.1%). The rest of diagnostic models showed a worse accuracy (accuracy = 41.67%¿63.89% and AUROC = 41.43%¿63.13%). Data Conclusion: The model combining the texture features from T2W and ADC map images with the semi-quantitative parameters from DW and DCE series allow the preoperative estimation of myometrial invasion. Evidence Level: 4 Technical Efficacy: Stage 3This study received funding from the Global Investigator Initiated Research Committee (GIIRC) research program by Bracco S.p.A (2015/0724). The funders had no role in study design, data collection and analysis and preparation of the manuscript.Rodriguez Ortega, A.; Alegre, A.; Lago, V.; Carot Sierra, JM.; Ten-Esteve, A.; Montoliu, G.; Domingo, S.... (2021). Machine Learning-Based Integration of Prognostic Magnetic Resonance Imaging Biomarkers for Myometrial Invasion Stratification in Endometrial Cancer. Journal of Magnetic Resonance Imaging. 54(3):987-995. https://doi.org/10.1002/jmri.27625S98799554

Coordination between growth and stress responses by DELLA in the liverwort Marchantia polymorpha

Plant survival depends on the optimal use of resources under variable environmental conditions. Among the mechanisms that mediate the balance between growth, differentiation, and stress responses, the regulation of transcriptional activity by DELLA proteins stands out. In angiosperms, DELLA accumulation promotes defense against biotic and abiotic stress and represses cell division and expansion, while the loss of DELLA function is associated with increased plant size and sensitivity toward stress.1 Given that DELLA protein stability is dependent on gibberellin (GA) levels2 and GA metabolism is influenced by the environment,3 this pathway is proposed to relay environmental information to the transcriptional programs that regulate growth and stress responses in angiosperms.4,5 However, DELLA genes are also found in bryophytes, whereas canonical GA receptors have been identified only in vascular plants.6, 7, 8, 9, 10 Thus, it is not clear whether these regulatory functions of DELLA predated or emerged with typical GA signaling. Here, we show that, as in vascular plants, the only DELLA in the liverwort Marchantia polymorpha also participates in the regulation of growth and key developmental processes and promotes oxidative stress tolerance. Moreover, part of these effects is likely caused by the conserved physical interaction with the MpPIF transcription factor. Therefore, we suggest that the role in the coordination of growth and stress responses was already encoded in the DELLA protein of the common ancestor of land plants, and the importance of this function is underscored by its conservation over the past 450 million years

Biofilm inhibition of pathogenic strains by extracellular products (ECPS) of Shewanella.sp Probiotic

Shewanella putrefaciens Pdp11 and V.proteolyticus DCF 12.2 are strains isolated by our research group. Recent works have been focused in the identification of substances as alternative of anti-biofilm methods and their implication in surface attachment inhibition. In this research, S. putrefaciens Pdp11 and V.proteolyticus DCF 12.2 have been cultured under different growth conditions (temperature, culture media and during 24 and 48 hours of incubation) and their extracellular products (ECPs) have been extracted and tested as potential postbiotics that affect the biofilm formation of several fish pathogenic strains. This assay results evidence that Pdp11_Pmix_2324 ECPs have showed the most impact in the biofilm formation of pathogenic strains. Therefore, ECPs secreted by Pdp11 and V.proteolyticus DCF 12.2 are implicated in the inhibition to adhesion of pathogens on surfaces.Proyecto de investigación PID2020-113637RB-C22. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A genetic approach reveals different modes of action of prefoldins

[EN] The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved in eukaryotes and is composed of subunits PFD1-6, and PFDc-TRiC/CCT folds actin and tubulins. PFDs also participate in a wide range of cellular processes, both in the cytoplasm and in the nucleus, and their malfunction causes developmental alterations and disease in animals and altered growth and environmental responses in yeast and plants. Genetic analyses in yeast indicate that not all of their functions require the canonical complex. The lack of systematic genetic analyses in plants and animals, however, makes it difficult to discern whether PFDs participate in a process as the canonical complex or in alternative configurations, which is necessary to understand their mode of action. To tackle this question, and on the premise that the canonical complex cannot be formed if one subunit is missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient in the six PFDs and compared various growth and environmental responses with those of the individual mutants. In this way, we demonstrate that the PFDc is required for seed germination, to delay flowering, or to respond to high salt stress or low temperature, whereas at least two PFDs redundantly attenuate the response to osmotic stress. A coexpression analysis of differentially expressed genes in the sextuple mutant identified several transcription factors, including ABA INSENSITIVE 5 (ABI5) and PHYTOCHROME-INTERACTING FACTOR 4, acting downstream of PFDs. Furthermore, the transcriptomic analysis allowed assigning additional roles for PFDs, for instance, in response to higher temperature.This work was supported by grants from the Spanish Ministry of Economy and Competitiveness and "Agencia Estatal de Investigacion"/FEDER/European Union (BIO2013-43184-P to D.A. and M.A.B., and BIO2016-79133-P and PID2019-109925GB-I00 to D.A.). N.B.-T., A.S.-M., and A.P.-A. were recipient of Ministerio de Economia y Competitividad (BES-2014-068868), EU MSCA-IF (H2020-MSCA-IF-2016746396) and Ministerio de Educacion (FPU17/05186) fellowships, respectively.Esteve-Bruna, D.; Blanco-Touriñán, N.; Serrano-Mislata, A.; Esquinas-Ariza, RM.; Resentini, F.; Forment Millet, JJ.; Carrasco-López, C.... (2021). A genetic approach reveals different modes of action of prefoldins. Plant Physiology. 187(3):1534-1550. https://doi.org/10.1093/plphys/kiab348S15341550187

Differentiation syndrome in patients with acute promyelocytic leukemia treated with all- trans retinoic acid and anthracycline chemotherapy: Characteristics, outcome, and prognostic factors

Differentiation syndrome (DS) can be a life-threatening complication in patients with acute promyelocytic leukemia (APL) undergoing induction therapy with all- trans retinoic acid (ATRA). Detailed knowl- edge about DS has remained limited. We present an analysis of the incidence, char- acteristics, prognostic factors, and out- come of 739 APL patients treated with ATRA plus idarubicin in 2 consecutive trials (Programa Espanol de Tratamientos en Hematologíc [PETHEMA] LPA96 and LPA99). Overall, 183 patients (24.8%) ex- perienced DS, 93 with a severe form (12.6%) and 90 with a moderate form (12.2%). Severe but not moderate DS was associated with an increase in mortality. A bimodal incidence of DS was observed, with peaks occurring in the first and third weeks after the start of ATRA therapy. A multivariate analysis indicated that a WBC count greater than 5 x 109/L and an abnor- mal serum creatinine level correlated with an increased risk of developing severe DS. Patients receiving systematic pred- nisone prophylaxis (LPA99 trial) in con- trast to those receiving selective prophy- laxis with dexamethasone (LPA96 trial) had a lower incidence of severe DS. Pa- tients developing severe DS showed a reduced 7-year relapse-free survival in the LPA96 trial (60% vs 85%, P = .003), but this difference was not apparent in the LPA99 trial

Hyperoxemia and excess oxygen use in early acute respiratory distress syndrome : Insights from the LUNG SAFE study

Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.Background: Concerns exist regarding the prevalence and impact of unnecessary oxygen use in patients with acute respiratory distress syndrome (ARDS). We examined this issue in patients with ARDS enrolled in the Large observational study to UNderstand the Global impact of Severe Acute respiratory FailurE (LUNG SAFE) study. Methods: In this secondary analysis of the LUNG SAFE study, we wished to determine the prevalence and the outcomes associated with hyperoxemia on day 1, sustained hyperoxemia, and excessive oxygen use in patients with early ARDS. Patients who fulfilled criteria of ARDS on day 1 and day 2 of acute hypoxemic respiratory failure were categorized based on the presence of hyperoxemia (PaO2 > 100 mmHg) on day 1, sustained (i.e., present on day 1 and day 2) hyperoxemia, or excessive oxygen use (FIO2 ≥ 0.60 during hyperoxemia). Results: Of 2005 patients that met the inclusion criteria, 131 (6.5%) were hypoxemic (PaO2 < 55 mmHg), 607 (30%) had hyperoxemia on day 1, and 250 (12%) had sustained hyperoxemia. Excess FIO2 use occurred in 400 (66%) out of 607 patients with hyperoxemia. Excess FIO2 use decreased from day 1 to day 2 of ARDS, with most hyperoxemic patients on day 2 receiving relatively low FIO2. Multivariate analyses found no independent relationship between day 1 hyperoxemia, sustained hyperoxemia, or excess FIO2 use and adverse clinical outcomes. Mortality was 42% in patients with excess FIO2 use, compared to 39% in a propensity-matched sample of normoxemic (PaO2 55-100 mmHg) patients (P = 0.47). Conclusions: Hyperoxemia and excess oxygen use are both prevalent in early ARDS but are most often non-sustained. No relationship was found between hyperoxemia or excessive oxygen use and patient outcome in this cohort. Trial registration: LUNG-SAFE is registered with ClinicalTrials.gov, NCT02010073publishersversionPeer reviewe