Expression of Intratumoral IGF-II Is Regulated by the Gene Imprinting Status in Triple Negative Breast Cancer from Vietnamese Patients

African American women suffer higher incidence and mortality of triple negative breast cancer (TNBC) than Caucasian women. TNBC is very aggressive, causing the worst clinical outcome. We previously demonstrated that tumors from these patients express high IGF-II and exhibit high activation of the IGF signaling pathways. IGF-II gene expression is imprinted (monoallelic), promotes tumor progression, and metastasis and regulates Survivin, a TNBC prognostic marker. Since BC mortality has increased among young Vietnamese women, we analyzed 48 (paired) TNBC samples from Vietnamese patients to assess IGF-II expression. We analyzed all samples by qrtPCR for identification of IGF-II heterozygosity and to determine allelic expression of the IGF-II gene. We also analyzed the tissues for proIGF-II and Survivin by RT-PCR and Western blotting. A total of 28 samples displayed IGF-II heterozygosity of which 78% were biallelic. Tumors with biallelic IGF-II gene expression exhibited the highest levels of proIGF-II and Survivin. Although 100% of these tissues corresponding normal samples were biallelic, they expressed significantly lower levels of or no proIGF-II and Survivin. Thus, IGF-II biallelic gene expression is differentially regulated in normal versus tumor tissues. We propose that intratumoral proIGF-II is dependent on the IGF-II gene imprinting status and it will promote a more aggressive TNBC

La mujer y la adversidad ambiental: un análisis del evento de precipitación intensa localizada en Montevideo

Estamos transitando una crisis socio-económica y ambiental a nivel global, como consecuencia del modelo actual de desarrollo. En este contexto, las repercusiones del cambio climático comienzan a manifestarse con más frecuencia y afectan en mayor medida a las personas en estado de vulnerabilidad. La Organización de las Naciones Unidas explicita que en estas situaciones de desastre ambiental las mujeres y las niñas son las más perjudicadas. Asimismo, cuestiona los instrumentos de recolección de datos, debido a la carencia de cifras desagregadas por sexo, edad y discapacidad, no siendo posible estimar cuántas mujeres y niñas se ven perjudicadas ante estas situaciones. En este contexto, el objetivo es investigar en una zona vulnerable de Montevideo contigua al arroyo Manga la repercusión de un evento particular de inundación ocurrido en enero de 2022, incorporando una mirada feminista. El resultado esperado será, por un lado, visibilizar los desafíos y limitaciones que enfrentan mujeres y niñas ante estas problemáticas y, por otro, evidenciar la necesidad de proponer nuevas formas de producir y obtener datos que incorporen la perspectiva de género en el análisis del territorio y en la generación de políticas públicas para una construcción de ciudades más igualitarias, justas y democráticas.Instituto de Investigaciones en Humanidades y Ciencias Sociale

HLA-C genotype and TCR vβ expression analysis in Mexican patients with Psoriasis

"Genetic background and T-cell expansion have been associated as the most important factors for psoriasis susceptibility in the Caucasian population. This study was performed to identify the T cell receptor Vβ repertoire and HLA-Cw genotype in two Mexican groups with severe chronic plaque-type psoriasis. HLA-C typing was performed to detect the allele pattern associated with the disease by sequence-specific primer-polymerase chain reaction. In parallel, RT-PCR and Western blot were used for the identification of the TCR Vβ repertoire. We found a wide variety of HLA-C alleles displayed with a preference to HLA-Cw *07 as the most representative allele in the group of patients. TCR Vβ-2 and Vβ-7 clone-type frequencies were statistically significant (p of 0.0280) when compared to other TCR Vβ expressed in the two groups. We found notable differences both in the HLA-C genotype and TCR Vβ repertoire in the groups of patients studied. Since Mexican individuals are genetically different from the Caucasian population, we suggest that due to these differences the susceptibility to disease and activation of T-cells for a proper immune response may be affected.

Diet in Peru's Pre-Hispanic Central Coast.

The Tablada de Lurín cemetery (200 BC – AD 200; Lima, Peru) is characterised by two mortuary phases. Based on associated grave finds and the lack of habitation sites near the cemetery, it has been hypothesised that both burial populations came from a certain distance of the site (ca. 20 km) and that they relied on land rather than marine resources. We tested these hypotheses, based on material culture, through stable isotope analysis. The aim was to understand the populations’ diet and geographic origins. We sampled 47 human individuals and eleven sets of faunal remains from both phases for stable isotope analysis (carbon, nitrogen, sulphur and oxygen) of bone and dental collagen, and apatite. Modern samples of autochthonous food were also tested as a baseline for comparison. The results showed preservation differences between the remains from both phases. Individuals from Phase 1 provided the best isotopic dataset and showed consumption of protein from marine resources and C4 plants. On the other hand, bioapatite carbon and oxygen stable isotope results from both phases highlighted differences in C4 plant consumption and individuals of possible non-local origin. The results underline the need to study further the effect of brewed or cooked beverages on bioapatite oxygen levels. Finally, results from Phase 1 fit with the broader dietary pattern evident in other Andean sites, where coastal populations consumed marine protein and C4 plants, as opposed to highland populations who relied on terrestrial protein sources and C3 plants

TOI-1199 b and TOI-1273 b: Two new transiting hot Saturns detected and characterized with SOPHIE and TESS

We report the characterization of two planet candidates detected by the Transiting Exoplanet Survey Satellite (TESS), TOI-1199 b and TOI-1273 b, with periods of 3.7 and 4.6 days, respectively. Follow-up observations for both targets, which include several ground-based light curves, confirmed the transit events. High-precision radial velocities from the SOPHIE spectrograph revealed signals at the expected frequencies and phases of the transiting candidates and allowed mass determinations with a precision of $8.4\%$ and $6.7\%$ for TOI-1199 b and TOI-1273 b, respectively. The planetary and orbital parameters were derived from a joint analysis of the radial velocities and photometric data. We find that the planets have masses of $0.239\,\pm\,0.020\,M_{\mathrm{J}}$ and $0.222\,\pm\,0.015\,M_{\mathrm{J}}$ and radii of $0.938\,\pm\,0.025\,R_{\mathrm{J}}$ and $0.99\,\pm\,0.22\,R_{\mathrm{J}}$, respectively. The grazing transit of TOI-1273 b translates to a larger uncertainty in its radius, and hence also in its bulk density, compared to TOI-1199 b. The inferred bulk densities of $0.358\,\pm\,0.041\,\mathrm{g}\,\mathrm{cm}^{-3}$ and $0.28\,\pm\,0.11\,\mathrm{g}\,\mathrm{cm}^{-3}$ are among the lowest known for exoplanets in this mass range, which, considering the brightness of the host stars ($V \approx 11\,\mathrm{mag}$), render them particularly amenable to atmospheric characterization via the transit spectroscopy technique. The better constraints on the parameters of TOI-1199 b provide a transmission spectroscopy metric of $134\,\pm\,17$, making it the better suited of the two planets for atmospheric studies.Comment: Accepted for publication in A&A. 20 pages, 7 tables, and 15 figure

Expression of the Stress Response Oncoprotein LEDGF/p75 in Human Cancer: A Study of 21 Tumor Types

Oxidative stress-modulated signaling pathways have been implicated in carcinogenesis and therapy resistance. The lens epithelium derived growth factor p75 (LEDGF/p75) is a transcription co-activator that promotes resistance to stress-induced cell death. This protein has been implicated in inflammatory and autoimmune conditions, HIV-AIDS, and cancer. Although LEDGF/p75 is emerging as a stress survival oncoprotein, there is scarce information on its expression in human tumors. The present study was performed to evaluate its expression in a comprehensive panel of human cancers. Transcript expression was examined in the Oncomine cancer gene microarray database and in a TissueScan Cancer Survey Panel quantitative polymerase chain reaction (Q-PCR) array. Protein expression was assessed by immunohistochemistry (IHC) in cancer tissue microarrays (TMAs) containing 1735 tissues representing single or replicate cores from 1220 individual cases (985 tumor and 235 normal tissues). A total of 21 major cancer types were analyzed. Analysis of LEDGF/p75 transcript expression in Oncomine datasets revealed significant upregulation (tumor vs. normal) in 15 out of 17 tumor types. The TissueScan Cancer Q-PCR array revealed significantly elevated LEDGF/p75 transcript expression in prostate, colon, thyroid, and breast cancers. IHC analysis of TMAs revealed significant increased levels of LEDGF/p75 protein in prostate, colon, thyroid, liver and uterine tumors, relative to corresponding normal tissues. Elevated transcript or protein expression of LEDGF/p75 was observed in several tumor types. These results further establish LEDGF/p75 as a cancer-related protein, and provide a rationale for ongoing studies aimed at understanding the clinical significance of its expression in specific human cancers

Envejecimiento de la población

•Actividades básicas de la vida diaria en personas mayores y factores asociados •Asociación entre depresión y posesión de mascotas en personas mayores •Calidad de vida en adultos mayores de Santiago aplicando el instrumento WHOQOL-BREF •Calidad de vida en usuarios con enfermedad de Parkinson, demencia y sus cuidadores, comuna de Vitacura •Caracterización de egresos hospitalarios de adultos mayores en Puerto Natales (2007-2009) •Comportamiento de las patologías incluidas como GES para el adulto mayor atendido en un Cesfam •Contribución de vitaminas y minerales a las ingestas recomendadas diarias en ancianos institucionalizados de Madrid •Estado de salud oral del paciente inscrito en el Programa de Visita Domiciliaria •Evaluación del programa de discapacidad severa en Casablanca con la matriz de marco lógico •Factores asociados a satisfacción vital en una cohorte de adultos mayores de Santiago, Chile •Pauta instrumental para la identificación de riesgos para el adulto mayor autovalente, en su vivienda •Perfil farmacológico del paciente geriátrico institucionalizado y posibles consecuencias en el deterioro cognitivo •Programa de cuidados paliativos y alivio del dolor en Puerto Natales •Rehabilitación mandibular implantoprotésica: efecto en calidad de vida relacionada con salud bucal en adultos mayores •Salud bucodental en adultos mayores autovalentes de la Región de Valparaíso •Transición epidemiológica y el estudio de carga de enfermedad en Brasi

Risk factors associated with adverse fetal outcomes in pregnancies affected by Coronavirus disease 2019 (COVID-19): a secondary analysis of the WAPM study on COVID-19.

Objectives To evaluate the strength of association between maternal and pregnancy characteristics and the risk of adverse perinatal outcomes in pregnancies with laboratory confirmed COVID-19. Methods Secondary analysis of a multinational, cohort study on all consecutive pregnant women with laboratory-confirmed COVID-19 from February 1, 2020 to April 30, 2020 from 73 centers from 22 different countries. A confirmed case of COVID-19 was defined as a positive result on real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of nasal and pharyngeal swab specimens. The primary outcome was a composite adverse fetal outcome, defined as the presence of either abortion (pregnancy loss before 22 weeks of gestations), stillbirth (intrauterine fetal death after 22 weeks of gestation), neonatal death (death of a live-born infant within the first 28 days of life), and perinatal death (either stillbirth or neonatal death). Logistic regression analysis was performed to evaluate parameters independently associated with the primary outcome. Logistic regression was reported as odds ratio (OR) with 95% confidence interval (CI). Results Mean gestational age at diagnosis was 30.6+/-9.5 weeks, with 8.0% of women being diagnosed in the first, 22.2% in the second and 69.8% in the third trimester of pregnancy. There were six miscarriage (2.3%), six intrauterine device (IUD) (2.3) and 5 (2.0%) neonatal deaths, with an overall rate of perinatal death of 4.2% (11/265), thus resulting into 17 cases experiencing and 226 not experiencing composite adverse fetal outcome. Neither stillbirths nor neonatal deaths had congenital anomalies found at antenatal or postnatal evaluation. Furthermore, none of the cases experiencing IUD had signs of impending demise at arterial or venous Doppler. Neonatal deaths were all considered as prematurity-related adverse events. Of the 250 live-born neonates, one (0.4%) was found positive at RT-PCR pharyngeal swabs performed after delivery. The mother was tested positive during the third trimester of pregnancy. The newborn was asymptomatic and had negative RT-PCR test after 14 days of life. At logistic regression analysis, gestational age at diagnosis (OR: 0.85, 95% CI 0.8-0.9 per week increase; pPeer reviewe

Role of age and comorbidities in mortality of patients with infective endocarditis

[Purpose]: The aim of this study was to analyse the characteristics of patients with IE in three groups of age and to assess the ability of age and the Charlson Comorbidity Index (CCI) to predict mortality. [Methods]: Prospective cohort study of all patients with IE included in the GAMES Spanish database between 2008 and 2015.Patients were stratified into three age groups:<65 years,65 to 80 years,and ≥ 80 years.The area under the receiver-operating characteristic (AUROC) curve was calculated to quantify the diagnostic accuracy of the CCI to predict mortality risk. [Results]: A total of 3120 patients with IE (1327 < 65 years;1291 65-80 years;502 ≥ 80 years) were enrolled.Fever and heart failure were the most common presentations of IE, with no differences among age groups.Patients ≥80 years who underwent surgery were significantly lower compared with other age groups (14.3%,65 years; 20.5%,65-79 years; 31.3%,≥80 years). In-hospital mortality was lower in the <65-year group (20.3%,<65 years;30.1%,65-79 years;34.7%,≥80 years;p < 0.001) as well as 1-year mortality (3.2%, <65 years; 5.5%, 65-80 years;7.6%,≥80 years; p = 0.003).Independent predictors of mortality were age ≥ 80 years (hazard ratio [HR]:2.78;95% confidence interval [CI]:2.32–3.34), CCI ≥ 3 (HR:1.62; 95% CI:1.39–1.88),and non-performed surgery (HR:1.64;95% CI:11.16–1.58).When the three age groups were compared,the AUROC curve for CCI was significantly larger for patients aged <65 years(p < 0.001) for both in-hospital and 1-year mortality. [Conclusion]: There were no differences in the clinical presentation of IE between the groups. Age ≥ 80 years, high comorbidity (measured by CCI),and non-performance of surgery were independent predictors of mortality in patients with IE.CCI could help to identify those patients with IE and surgical indication who present a lower risk of in-hospital and 1-year mortality after surgery, especially in the <65-year group