A new Mean Dynamic Topography of the Mediterranean Sea based on model outputs, drifter data, hydrological profiles and altimeter measurements

Trabajo presentado en la EGU General Assemby 2013, celebrada del 7 al 12 de abril de 2013 en Viena (Austria)Peer Reviewe

Colonization of the Mediterranean Basin by the vector biting midge species Culicoides imicola: an old story

Understanding the demographic history and genetic make-up of colonizing species is critical for inferring population sources and colonization routes. This is of main interest for designing accurate control measures in areas newly colonized by vector species of economically important pathogens. The biting midge Culicoides imicola is a major vector of Orbiviruses to livestock. Historically, the distribution of this species was limited to the Afrotropical region. Entomological surveys first revealed the presence of C. imicola in the south of the Mediterranean basin by the 1970's. Following recurrent reports of massive bluetongue outbreaks since the 1990s, the presence of the species was confirmed in northern areas. In this study, we addressed the chronology and processes of C. imicola colonization in the Mediterranean basin. We characterized the genetic structure of its populations across Mediterranean and African regions using both mitochondrial and nuclear markers, and combined phylogeographical analyses with population genetics and approximate Bayesian computation. We found a west/east genetic differentiation between populations, occurring both within Africa and within the Mediterranean basin. We demonstrated that three of these groups had experienced demographic expansions in the Pleistocene, probably because of climate changes during this period. Finally, we showed that C. imicola could have colonized the Mediterranean basin in the late Pleistocene or early Holocene through a single event of introduction; however we cannot exclude the hypothesis involving two routes of colonization. Thus, the recent bluetongue outbreaks are not linked to C. imicola colonization event, but rather to biological changes in the vector or the virus

SKIM, a candidate satellite mission exploring global ocean currents and waves

The Sea surface KInematics Multiscale monitoring (SKIM) satellite mission is designed to explore ocean surface current and waves. This includes tropical currents, notably the poorly known patterns of divergence and their impact on the ocean heat budget, and monitoring of the emerging Arctic up to 82.5°N. SKIM will also make unprecedented direct measurements of strong currents, from boundary currents to the Antarctic circumpolar current, and their interaction with ocean waves with expected impacts on air-sea fluxes and extreme waves. For the first time, SKIM will directly measure the ocean surface current vector from space. The main instrument on SKIM is a Ka-band conically scanning, multi-beam Doppler radar altimeter/wave scatterometer that includes a state-of-the-art nadir beam comparable to the Poseidon-4 instrument on Sentinel 6. The well proven Doppler pulse-pair technique will give a surface drift velocity representative of the top meter of the ocean, after subtracting a large wave-induced contribution. Horizontal velocity components will be obtained with an accuracy better than 7 cm/s for horizontal wavelengths larger than 80 km and time resolutions larger than 15 days, with a mean revisit time of 4 days for of 99% of the global oceans. This will provide unique and innovative measurements that will further our understanding of the transports in the upper ocean layer, permanently distributing heat, carbon, plankton, and plastics. SKIM will also benefit from co-located measurements of water vapor, rain rate, sea ice concentration, and wind vectors provided by the European operational satellite MetOp-SG(B), allowing many joint analyses. SKIM is one of the two candidate satellite missions under development for ESA Earth Explorer 9. The other candidate is the Far infrared Radiation Understanding and Monitoring (FORUM). The final selection will be announced by September 2019, for a launch in the coming decade

Optimization of proximity ligation assay (PLA) for detection of protein interactions and fusion proteins in non-adherent cells: application to pre-B lymphocytes

International audienceBackground: Genetic abnormalities, including chromosomal translocations, are described for many hematological malignancies. From the clinical perspective, detection of chromosomal abnormalities is relevant not only for diagnostic and treatment purposes but also for prognostic risk assessment. From the translational research perspective, the identification of fusion proteins and protein interactions has allowed crucial breakthroughs in understanding the pathogenesis of malignancies and consequently major achievements in targeted therapy. Methods: We describe the optimization of the Proximity Ligation Assay (PLA) to ascertain the presence of fusion proteins, and protein interactions in non-adherent pre-B cells. PLA is an innovative method of protein-protein colocalization detection by molecular biology that combines the advantages of microscopy with the advantages of molecular biology precision, enabling detection of protein proximity theoretically ranging from 0 to 40 nm. Results: We propose an optimized PLA procedure. We overcome the issue of maintaining non-adherent hematological cells by traditional cytocentrifugation and optimized buffers, by changing incubation times, and modifying washing steps. Further, we provide convincing negative and positive controls, and demonstrate that optimized PLA procedure is sensitive to total protein level. The optimized PLA procedure allows the detection of fusion proteins and protein interactions on non-adherent cells. Conclusion: The optimized PLA procedure described here can be readily applied to various non-adherent hematological cells, from cell lines to patients' cells. The optimized PLA protocol enables detection of fusion proteins and their subcellular expression, and protein interactions in non-adherent cells. Therefore, the optimized PLA protocol provides a new tool that can be adopted in a wide range of applications in the biological field

Removing Spurious Low-Frequency Variability in Drifter Velocities

ABSTRACT Satellite-tracked drifting buoys of the Global Drifter Program have drogues, centered at 15-m depth, to minimize direct wind forcing and Stokes drift. Drogue presence has historically been determined from submergence or tether strain records. However, recent studies have revealed that a significant fraction of drifters believed to be drogued have actually lost their drogues, a problem that peaked in the mid-2000s before the majority of drifters in the global array switched from submergence to tether strain sensors. In this study, a methodology is applied to the data to automatically reanalyze drogue presence based on anomalous downwind ageostrophic motion. Results indicate that the downwind slip of undrogued drifters is approximately 50% higher than previously believed. The reanalyzed results no longer exhibit the dramatic and spurious interannual variations seen in the original data. These results, along with information from submergence/tether strain and transmission frequency variations, are now being used to conduct a systematic manual reevaluation of drogue presence for each drifter in the post-1992 dataset

Mean Dynamic Topography of the Ocean Derived from Satellite and Drifting Buoy Data Using Three Different Techniques

Presented here are three mean dynamic topography maps derived with different methodologies. The first method combines sea level observed by the high-accuracy satellite radar altimetry with the geoid model of the Gravity Recovery and Climate Experiment (GRACE), which has recently measured the earth’s gravity with unprecedented spatial resolution and accuracy. The second one synthesizes near-surface velocities from a network of ocean drifters, hydrographic profiles, and ocean winds sorted according to the horizontal scales. In the third method, these global datasets are used in the context of the ocean surface momentum balance. The second and third methods are used to improve accuracy of the dynamic topography on fine space scales poorly resolved in the first method. When they are used to compute a multiyear time-mean global ocean surface circulation on a 0.5° horizontal resolution, both contain very similar, new small-scale midocean current patterns. In particular, extensions of western boundary currents appear narrow and strong despite temporal variability and exhibit persistent meanders and multiple branching. Also, the locations of the velocity concentrations in the Antarctic Circumpolar Current become well defined. Ageostrophic velocities reveal convergent zones in each subtropical basin. These maps present a new context in which to view the continued ocean monitoring with in situ instruments and satellites

Ocean Circulation from Space

International audienceThis paper reviews the recent progress in our estimation of ocean dynamic topography and the derived surface geostrophic currents, mainly based on multiple nadir radar altimeter missions. These altimetric observations provide the cornerstone of our ocean circulation observing system from space. The largest signal in sea surface topography is from the mean surface dominated by the marine geoid, and we will discuss recent progress in observing the mean ocean circulation from altimetry, once the geoid and other corrections have been estimated and removed. We then address the recent advances in our observations of the large-scale and mesoscale ocean circulation from space, and the particular challenges and opportunities for new observations in the polar regions. The active research in the ocean barotropic tides and internal tidal circulation is also presented. The paper also addresses how our networks of global multi-satellite and in situ observations are being combined and assimilated to characterize the four-dimensional ocean circulation, for climate research and ocean forecasting systems. For the future of ocean circulation from space, the need for continuity of our current observing system is crucial, and we discuss the exciting enhancement to come with global wide-swath altimetry, the extension into the coastal and high-latitude regions, and proposals for direct total surface current satellites in the 2030 period