The energy biology of European Mistletoe (Viscum album)

The hemiparasitic European mistletoe (Viscum album) is known for its extraordinary way of life. Not only its huge genome of about 90 Gbp is noticeable, but also the absence of mitochondrial complex I of the Oxidative Phosphorylation system. Since a large genome indicates a high energy demand during cellular division, absence of complex I, which strongly contributes to the proton gradient across the inner mitochondrial membrane and thus to ATP production, is to be considered remarkable. How can V. album accomplish its energy metabolism? This is the central research question of this thesis. To this end, the transcriptome of V. album was first sequenced to provide the basis for efficient proteome analysis. RNA was isolated from mistletoe leaves, flowers, and stems harvested in summer and winter. The RNA was next transcribed into cDNA and sequenced as a pooled sample via the PacBio sequencing strategy. The resulting initial Viscum album Gene Space (VaGs) database showed 78% completeness based on Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis. To further develop this database, additional Illumina sequencing of the individual samples (summer and winter) was performed. The resulting Viscum album Gene Space database II (VaGsII) has a completeness of 93% and contains sequences of 90,039 transcripts. Based on these sequences, a GC content of 50% could be calculated. This is an unusually high GC content, as in other dicotyledonous plants the GC content usually ranges between 43-45 %. Due to the resulting enhanced stability of the DNA, an increased energy requirement must also be anticipated for DNA replication and transcription. In addition to the absence of the mitochondrial genes encoding subunits of complex I, the absence of almost all nuclear genes encoding complex I subunits could be shown. Furthermore, by re-evaluating an existing complexome dataset of V. album mitochondria using the new VaGs II database, more than 1,000 additional mitochondrial proteins could be identified with respect to the original evaluation. Besides the mitochondria, also the chloroplasts were examined in more detail to determine their contribution to the energy metabolism of V. album cells through photosynthesis and photophosphorylation. In the course of this examination, a complete absence of the NDH complex (NADH dehydrogenase- like complex, chloroplast pendant of mitochondrial complex I), which contributes to cyclic electron transport around photosystem I, was proven on the proteome level. In addition, PGR5 and PGRL1, two proteins which were shown to be alternatively involved in cyclic electron transport around photosystem I, were found to be of reduced abundance in V. album compared to the model plant Arabidopsis thaliana. Abundance of the chloroplast ATP synthase complex is comparable to A. thaliana; however, its stability clearly is increased in V. album. Also, the photosystem II is of similar abundance in A. thaliana and V. album, in contrast to the photosystem I, which is of comparatively low abundance in V. album. It can be concluded that both, linear and cyclic electron transport and thus ATP synthesis by photophosphorylation are comparatively low in V. album. In summary, it can be concluded that: 1. V. album has an even higher energy demand than previously thought due to its high GC content. 2. ATP production in mitochondria and chloroplasts is limited due to the absence or reduced abundance of some of the involved proteins and protein complexes. How sufficient amounts of ATP are provided in V. album cells is therefore still not entirely clear. It is hypothesized that the slow growth and reduced cell division rate of V. album might reduce its energy demand. In addition, sugar compounds transported in the host xylem in spring may be a source of energy for V. album. This may also explain the strong growth rate of V. album in spring. Further research is needed to understand the way of life of this very particular plant

The photosynthesis apparatus of European mistletoe (Viscum album)

European mistletoe (Viscum album) is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (Complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). Here, we present an investigation of the V. album energy metabolism taking place in chloroplasts. Thylakoids were purified from young V. album leaves, and membrane-bound protein complexes were characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome profiling approach. Proteins were systematically identified by label-free quantitative shotgun proteomics. We identified 41,800 distinct proteins (accessible at https://complexomemap.de/va_leaves), including nearly 100 proteins forming part of the protein complexes involved in the light-dependent part of photosynthesis. The photosynthesis apparatus of V. album has distinct features: (1) comparatively low amounts of Photosystem I; (2) absence of the NDH complex (the chloroplast pendant of mitochondrial Complex I involved in cyclic electron transport (CET) around Photosystem I); (3) reduced levels of the proton gradient regulation 5 (PGR5) and proton gradient regulation 5-like 1 (PGRL1) proteins, which offer an alternative route for CET around Photosystem I; (4) comparable amounts of Photosystem II and the chloroplast ATP synthase complex to other seed plants. Our data suggest a restricted capacity for chloroplast ATP biosynthesis by the photophosphorylation process. This is in addition to the limited ATP supply by the mitochondria. We propose a view on mistletoe’s mode of life, according to which its metabolism relies to a greater extent on energy-rich compounds provided by the host trees

The gene space of European mistletoe (Viscum album)

European mistletoe (Viscum album) is a hemiparasitic flowering plant that is known for its very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5′ and 3′ non-coding regions). mRNA fractions were isolated from different V. album organs harvested in summer or winter and were analyzed via single-molecule real-time sequencing. We determined sequences of 39 092 distinct open reading frames encoding 32 064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry-based data of the V. album mitochondrial proteome, which previously had been evaluated using the A. thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI

The Viscum album Gene Space database

The hemiparasitic flowering plant Viscum album (European mistletoe) is known for its very special life cycle, extraordinary biochemical properties, and extremely large genome. The size of its genome is estimated to be 30 times larger than the human genome and 600 times larger than the genome of the model plant Arabidopsis thaliana. To achieve insights into the Gene Space of the genome, which is defined as the space including and surrounding protein-coding regions, a transcriptome project based on PacBio sequencing has recently been conducted. A database resulting from this project contains sequences of 39,092 different open reading frames encoding 32,064 distinct proteins. Based on ‘Benchmarking Universal Single-Copy Orthologs’ (BUSCO) analysis, the completeness of the database was estimated to be in the range of 78%. To further develop this database, we performed a transcriptome project of V. album organs harvested in summer and winter based on Illumina sequencing. Data from both sequencing strategies were combined. The new V. album Gene Space database II (VaGs II) contains 90,039 sequences and has a completeness of 93% as revealed by BUSCO analysis. Sequences from other organisms, particularly fungi, which are known to colonize mistletoe leaves, have been removed. To evaluate the quality of the new database, proteome data of a mitochondrial fraction of V. album were re-analyzed. Compared to the original evaluation published five years ago, nearly 1000 additional proteins could be identified in the mitochondrial fraction, providing new insights into the Oxidative Phosphorylation System of V. album. The VaGs II database is available at https://viscumalbum.pflanzenproteomik.de/. Furthermore, all V. album sequences have been uploaded at the European Nucleotide Archive (ENA)

The Viscum album Gene Space database

The hemiparasitic flowering plant Viscum album (European mistletoe) is known for its very special life cycle, extraordinary biochemical properties, and extremely large genome. The size of its genome is estimated to be 30 times larger than the human genome and 600 times larger than the genome of the model plant Arabidopsis thaliana. To achieve insights into the Gene Space of the genome, which is defined as the space including and surrounding protein-coding regions, a transcriptome project based on PacBio sequencing has recently been conducted. A database resulting from this project contains sequences of 39,092 different open reading frames encoding 32,064 distinct proteins. Based on ‘Benchmarking Universal Single-Copy Orthologs’ (BUSCO) analysis, the completeness of the database was estimated to be in the range of 78%. To further develop this database, we performed a transcriptome project of V. album organs harvested in summer and winter based on Illumina sequencing. Data from both sequencing strategies were combined. The new V. album Gene Space database II (VaGs II) contains 90,039 sequences and has a completeness of 93% as revealed by BUSCO analysis. Sequences from other organisms, particularly fungi, which are known to colonize mistletoe leaves, have been removed. To evaluate the quality of the new database, proteome data of a mitochondrial fraction of V. album were re-analyzed. Compared to the original evaluation published five years ago, nearly 1000 additional proteins could be identified in the mitochondrial fraction, providing new insights into the Oxidative Phosphorylation System of V. album. The VaGs II database is available at https://viscumalbum.pflanzenproteomik.de/. Furthermore, all V. album sequences have been uploaded at the European Nucleotide Archive (ENA)

Optimierungsansätze zur Verbesserung von Nachhaltigkeit, Ressourceneffizienz und Tierwohl in ökologischen und konventionellen Betrieben im Netzwerk Pilotbetriebe

In a network of organic and conventional pilot farms with dairy and/or arable production in Germany (www.pilotbetriebe.de) aspects of sustainability, resource efficiency, animal health and welfare and economic aspects are analysed. This is based on on-farm assessments and on whole farm modelling. In the first interdisciplinary workshops on the project farms results were presented and scenarios were developed in a participatory approach by scientists, consultants and farmers to improve N, P and land use efficiency as well as dairy health and welfare. Typical areas of action to optimize sustainability in these aspects were identified on the farms, e.g., complete accounting of manure N (N balance), enrichment of crop rotations with clover grass and cover crops (humus balance), improving forage quality (productivity, nutritional imbalances), improvement of housing conditions and grazing access (animal health and welfare). Systematic integration and detection of interconnectedness of environmental performance of production, animal welfare, resource efficiency and productivity result in a new quality in development of farm concepts

Sports graduate capabilities and competencies: a comparison of graduate and employer perceptions in six EU countries

The graduate employment market faces ever-increasing socio-economic and political pressures. Higher Education Institutions and the sport sector in the EU have an important role in contributing to graduate employment. The aims of the study were: (1) to assess general perceptions of employability, and (2) to assess sports graduates’ and employers’ perceptions of specific capabilities and competencies in order to identify possible improvements for sports graduate employability programmes. A cross-sectional survey of sports graduates and employers was administered in six EU countries including the UK, France, Germany, Spain, Greece and the Czech Republic to assess graduate and employer perceptions. A graduate capabilities and competencies framework was devised to assess personal, interpersonal, cognitive, role-specific and generic skills. Responses were elicited from 1132 sports graduates and 327 employers. There was generally a wide difference of opinion between employers and sports graduates in terms of the importance and possession of a number of capabilities and competencies. There is a need for the Higher Education sector and employers to take responsibility in ensuring that work experience, work placement and volunteering opportunities are embedded in curricula and to ensure the fitness of purpose of what and how graduate capabilities and competencies are assessed

Differential cross section measurements for the production of a W boson in association with jets in proton–proton collisions at √s = 7 TeV

Measurements are reported of differential cross sections for the production of a W boson, which decays into a muon and a neutrino, in association with jets, as a function of several variables, including the transverse momenta (pT) and pseudorapidities of the four leading jets, the scalar sum of jet transverse momenta (HT), and the difference in azimuthal angle between the directions of each jet and the muon. The data sample of pp collisions at a centre-of-mass energy of 7 TeV was collected with the CMS detector at the LHC and corresponds to an integrated luminosity of 5.0 fb[superscript −1]. The measured cross sections are compared to predictions from Monte Carlo generators, MadGraph + pythia and sherpa, and to next-to-leading-order calculations from BlackHat + sherpa. The differential cross sections are found to be in agreement with the predictions, apart from the pT distributions of the leading jets at high pT values, the distributions of the HT at high-HT and low jet multiplicity, and the distribution of the difference in azimuthal angle between the leading jet and the muon at low values.United States. Dept. of EnergyNational Science Foundation (U.S.)Alfred P. Sloan Foundatio

Penilaian Kinerja Keuangan Koperasi di Kabupaten Pelalawan

This paper describe development and financial performance of cooperative in District Pelalawan among 2007 - 2008. Studies on primary and secondary cooperative in 12 sub-districts. Method in this stady use performance measuring of productivity, efficiency, growth, liquidity, and solvability of cooperative. Productivity of cooperative in Pelalawan was highly but efficiency still low. Profit and income were highly, even liquidity of cooperative very high, and solvability was good

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis