The effect of amino acid supplementation on weight, body composition, muscle size, and muscle strength in amateur weightlifters

Fifteen amateur weightlifters, between the ages of 21 and 35 years, volunteered to participate in a study to determine the effect of an amino acid supplement on muscle size, muscle strength, body composition, and body weight. A blind protocol was followed in which eight of the participants were administered the amino acid supplement and seven received a placebo. Each subject was tested before and immediately after the ten week experimental period for height, weight, skinfolds, hydrostatic weighing, body circumferences, static strength, and dynamic strength; Post supplementation, independent T tests indicated that the differences in percent body fat, weight, circumference measurements, static strength, and dynamic strength between the amino acid group and the control group were not significant. It was, therefore, concluded that the amino acid supplement administered in this study had no measured effect on percent body fat, body weight, muscle size, and muscle strength of the participating subjects

Bridging the gap:a review of dose investigations in paediatric investigation plans

Aims In the EU, development of new medicines for children should follow a prospectively agreed paediatric investigation plan (PIP). Finding the right dose for children is crucial but challenging due to the variability of pharmacokinetics across age groups and the limited sample sizes available. We examined strategies adopted in PIPs to support paediatric dosing recommendations to identify common assumptions underlying dose investigations and the attempts planned to verify them in children. Methods We extracted data from 73 PIP opinions recently adopted by the Paediatric Committee of the European Medicines Agency. These opinions represented 79 medicinal development programmes and comprised a total of 97 dose investigation studies. We identified the design of these dose investigation studies, recorded the analyses planned and determined the criteria used to define target doses. Results Most dose investigation studies are clinical trials (83 of 97) that evaluate a single dosing rule. Sample sizes used to investigate dose are highly variable across programmes, with smaller numbers used in younger children (< 2 years). Many studies (40 of 97) do not pre-specify a target dose criterion. Of those that do, most (33 of 57 studies) guide decisions using pharmacokinetic data alone. Conclusions Common assumptions underlying dose investigation strategies include dose proportionality and similar exposure−response relationships in adults and children. Few development programmes pre-specify steps to verify assumptions in children. There is scope for the use of Bayesian methods as a framework for synthesizing existing information to quantify prior uncertainty about assumptions. This process can inform the design of optimal drug development strategies

How to trade electricity flexibility using artificial intelligence - An integrated algorithmic framework

In course of the energy transition, the growing share of Renewable Energy Sources (RES) makes electricity generation more decentralized and intermittent. This increases the relevance of exploiting flexibility potentials that help balancing intermittent RES supply and demand and, thus, contribute to overall system resilience. Digital technologies, in the form of automated trading algorithms, may considerably contribute to flexibility exploitation, as they enable faster and more accurate market interactions. In this paper, we develop an integrated algorithmic framework that finds an optimal trading strategy for flexibility on multiple markets. Hence, our work supports the trading of flexibility in a multi-market environment that results in enhanced market integration and harmonization of economically traded and physically delivered electricity, which finally promotes resilience in highly complex electricity systems

Round-trip migration and energy budget of a breeding female humpback whale in the Northeast Atlantic

In the northern hemisphere, humpback whales (Megaptera novaeangliae) typically migrate between summer/autumn feeding grounds at high latitudes, and specific winter/spring breeding grounds at low latitudes. Northeast Atlantic (NEA) humpback whales for instance forage in the Barents Sea and breed either in the West Indies, or the Cape Verde Islands, undertaking the longest recorded mammalian migration (~ 9 000 km). However, in the past decade hundreds of individuals have been observed foraging on herring during the winter in fjord systems along the northern Norwegian coast, with unknown consequences to their migration phenology, breeding behavior and energy budgets. Here we present the first complete migration track (321 days, January 8th, 2019—December 6th, 2019) of a humpback whale, a pregnant female that was equipped with a satellite tag in northern Norway. We show that whales can use foraging grounds in the NEA (Barents Sea, coastal Norway, and Iceland) sequentially within the same migration cycle, foraging in the Barents Sea in summer/fall and in coastal Norway and Iceland in winter. The migration speed was fast (1.6 ms-1), likely to account for the long migration distance (18 300 km) and long foraging season, but varied throughout the migration, presumably in response to the calf’s needs after its birth. The energetic cost of this migration was higher than for individuals belonging to other populations. Our results indicate that large whales can modulate their migration speed to balance foraging opportunities with migration phenology, even for the longest migrations and under the added constraint of reproduction

Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha

The ability to catalyze diverse reactions with relevance for chemical and pharmaceutical research and industry has led to an increasing interest in fungal enzymes. There is still an enormous potential considering the sheer amount of new enzymes from the huge diversity of fungi. Most of these fungal enzymes have not been characterized yet due to the lack of high throughput synthesis and analysis methods. This bottleneck could be overcome by means of cell-free protein synthesis. In this study, cell-free protein synthesis based on eukaryotic cell lysates was utilized to produce a functional glycoside hydrolase (GH78) from the soft-rot fungus Xylaria polymorpha (Ascomycota). The enzyme was successfully synthesized under different reaction conditions. We characterized its enzymatic activities and immobilized the protein via FLAG-Tag interaction. Alteration of several conditions including reaction temperature, template design and lysate supplementation had an influence on the activity of cell-free synthesized GH78. Consequently this led to a production of purified GH78 with a specific activity of 15.4 U mg− 1. The results of this study may be foundational for future high throughput fungal enzyme screenings, including substrate spectra analysis and mutant screenings

Consumer-centric electricity markets: Six design principles

peer reviewedKey to the necessary decarbonization of energy systems is the large-scale expansion of renewable energy sources and their integration into the electricity system. This integration is challenging because the feed-in from renewable energy sources is highly intermittent and largely dependent on uncontrollable factors such as weather patterns. To maintain grid stability, which refers to the required balance between demand and supply in the electricity system, flexibility is key. Large flexibility potentials can be found on the electricity demand side. However, current electricity market design in Europe, while providing major flexibility incentives, often neglects small-scale electricity consumers and distributed energy resources. We contribute to shape future electricity markets with consumers at the heart by developing six design principles for a consumer-centric electricity market design. We proceed by conducting a systematic literature review and evaluate the findings by expert interviews. Based on the developed design principles, we define a consumer-centric electricity market design as a set of market rules that align with the rules of other relevant energy markets and allow for the efficient matching of electricity demand and supply, with consumers having nondiscriminatory market access, being exposed to finegrained price signals, being able to express their preferences, and having sufficient possibilities to protect themselves against unexpected price spikes. By actively incorporating consumers into electricity markets, we contribute to the overarching goal of integrating renewable energy sources while promoting energy justice, i.e., supporting a balanced mix of economic, political, environmental, and social interests.9. Industry, innovation and infrastructur

Refraining from rights and giving in to personalised control: young unemployed peoples’ experiences and perceptions of public and third sector support in the UK and Norway

In this article, we present an analysis of young unemployed peoples’ perceptions and experiences with public and third sector support in Norway and the UK. Drawing on data generated through qualitative semi-structured interviews, the analysis shows that street level workers’ approaches towards clients are important in understanding(dis)engagement with various employment support systems. Taking a ‘street-level’- perspective as a lens for understanding young, unemployed peoples’ perceptions and experiences, we suggest that their voices as (potential) participants in employment services can contribute a further dimension to perspectives that are more usually focused on workers as performers of politics. The paper highlights the challenges of making employment services responsive to young peoples’ needs and expectations

Connection between dynamically derived initial mass function normalization and stellar population parameters

Date of Acceptance: 10/08/2014We report on empirical trends between the dynamically determined stellar initial mass function (IMF) and stellar population properties for a complete, volume-limited sample of 260 early-type galaxies from the ATLAS3D project. We study trends between our dynamically derived IMF normalization αdyn ≡ (M/L)stars/(M/L)Salp and absorption line strengths, and interpret these via single stellar population-equivalent ages, abundance ratios (measured as [α/Fe]), and total metallicity, [Z/H]. We find that old and alpha-enhanced galaxies tend to have on average heavier (Salpeter-like) mass normalization of the IMF, but stellar population does not appear to be a good predictor of the IMF, with a large range of αdyn at a given population parameter. As a result, we find weak αdyn-[α/Fe] and αdyn -Age correlations and no significant αdyn -[Z/H] correlation. The observed trends appear significantly weaker than those reported in studies that measure the IMF normalization via the low-mass star demographics inferred through stellar spectral analysis.Peer reviewe