Anomalous in vitro nitrate reductase activity of pea (Pisum sativum)

During the in vitro assay of nitrate reductase extracted from Pisum sativum, unusual kinetics of NO−2 accumulation were observed: in a 10-min assay nearly half of the total NO−2 appeared within 1min of the start of the reaction. The rapid NO−2 accumulation occurring optimally at pH8,3 was sensitive to KCN, length of enzyme pre-incubation time (in the absence of co-enzyme) and amount of NO−2 present, but was insensitive to low temperature. The amount of NO−2 accumulated equalled the amount of NO−2 lost during the assay

Логіко-лінгвістична модель як засіб відображення синтаксичних особливостей текстової інформації

Запропоновано використовувати логіко-лінгвістичні моделі як засіб відображення синтаксичних особливостей текстової інформації, перелічено та обґрунтовано правила їх формування, наведено алгоритм створення всіх складових таких моделей.Предложено использовать логико-лингвистические модели как способ отображения синтаксических особенностей текстовой информации, перечислены и обоснованы правила их формирования, приведен алгоритм создания всех составных таких моделей.It is suggested using a logico-linguistic model as a method of display the syntactical features of text information. There are listed and justified the rules for their forming and also are shown an algorithm of creation of all components of such models in this paper

Genetic Sharing with Cardiovascular Disease Risk Factors and Diabetes Reveals Novel Bone Mineral Density Loci.

Bone Mineral Density (BMD) is a highly heritable trait, but genome-wide association studies have identified few genetic risk factors. Epidemiological studies suggest associations between BMD and several traits and diseases, but the nature of the suggestive comorbidity is still unknown. We used a novel genetic pleiotropy-informed conditional False Discovery Rate (FDR) method to identify single nucleotide polymorphisms (SNPs) associated with BMD by leveraging cardiovascular disease (CVD) associated disorders and metabolic traits. By conditioning on SNPs associated with the CVD-related phenotypes, type 1 diabetes, type 2 diabetes, systolic blood pressure, diastolic blood pressure, high density lipoprotein, low density lipoprotein, triglycerides and waist hip ratio, we identified 65 novel independent BMD loci (26 with femoral neck BMD and 47 with lumbar spine BMD) at conditional FDR < 0.01. Many of the loci were confirmed in genetic expression studies. Genes validated at the mRNA levels were characteristic for the osteoblast/osteocyte lineage, Wnt signaling pathway and bone metabolism. The results provide new insight into genetic mechanisms of variability in BMD, and a better understanding of the genetic underpinnings of clinical comorbidity

Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry.

BACKGROUND: The Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. METHODS AND RESULTS: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012-2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P < 0.001). Diabetes and coronary artery disease (CAD) were prevalent in 36.2% and 28.1% of patients as compared with global prevalence of 22.2% and 21.6%, respectively (P < 0.001 for both). Antiplatelet therapy was the most common antithrombotic treatment in India. With increasing stroke risk, however, patients were more likely to receive oral anticoagulant therapy [mainly vitamin K antagonist (VKA)], but average international normalized ratio (INR) was lower among Indian patients [median INR value 1.6 (interquartile range {IQR}: 1.3-2.3) versus 2.3 (IQR 1.8-2.8) (P < 0.001)]. Compared with other countries, patients from India had markedly higher rates of all-cause mortality [7.68 per 100 person-years (95% confidence interval 6.32-9.35) vs 4.34 (4.16-4.53), P < 0.0001], while rates of stroke/systemic embolism and major bleeding were lower after 1 year of follow-up. CONCLUSION: Compared to previously published registries from India, the GARFIELD-AF registry describes clinical profiles and outcomes in Indian patients with AF of a different etiology. The registry data show that compared to the rest of the world, Indian AF patients are younger in age and have more diabetes and CAD. Patients with a higher stroke risk are more likely to receive anticoagulation therapy with VKA but are underdosed compared with the global average in the GARFIELD-AF. CLINICAL TRIAL REGISTRATION-URL: http://www.clinicaltrials.gov. Unique identifier: NCT01090362

40Ar/39Ar laserprobe direct dating of discrete deformational events: continuous record of Early Alpine tectonics in the Pelagonian Zone, NW Aegean area, Greece.

The Pelagonian Zone of the Hellenides, which occurs in the northwestern Aegean region of the Eastern Mediterranean, contains remnants of a high-pressure metamorphic belt which subsequently underwent localized reworking during low-temperature deformational events. Formation of Alpine deformational structures in the Ossa Massif (northern Pelagonian Zone) occurred at metamorphic temperatures which failed to reset most pre-Alpin