Impact Analysis of Loved One Peer Coaching on Persons with the Disease of Addiction

When considering the impacts of addiction on society, it is important to include loved ones (LOs) – close friends and family members of those suffering from the chronic disease. The societal burden placed on LOs, in addition to the stress and pain they already experience in the face of addiction, results in an extremely neglected and isolated population. Using deductive thematic analysis procedures, this paper examines the role of LO wellness in person with disease (PWD) recovery by reviewing the impact of peer coaching, a new form of support for LOs. Semi-structured interviews with seven PWD and LO clients of Face It TOGETHER (FIT), an addiction management nonprofit, were analyzed in this study. Five primary themes were identified: “extended stress and overcompensating,” “helplessness,” “improved communication,” “openness to resources” and “mutually beneficial.” Ultimately, peer coaching lead to better communication and relationships, lessened helplessness previously felt by LOs and improved LO and PWD wellness

Registration of \u27Manska\u27 Pubescent Intermediate Wheatgrass

\u27MANSKA\u27 pubescent intermediate wheatgrass [Thinopyrum intermedium subsp. barbulatum (Schur) Barkw. & Dewey] (Reg. no. CV-21, PI 562527) was tested as Mandan 12781 and released 16 April 1992 by the USDA-ARS in cooperation with the USDA-SCS; the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska; and the North Agricultural Experiment Station

Guanylyl cyclase activity associated with putative bifunctional integral membrane proteins in Plasmodium falciparum.

We report here that guanylyl cyclase activity is associated with two large integral membrane proteins (PfGCalpha and PfGCbeta) in the human malaria parasite Plasmodium falciparum. Unusually, the proteins appear to be bifunctional; their amino-terminal regions have strong similarity with P-type ATPases, and the sequence and structure of the carboxyl-terminal regions conform to that of G protein-dependent adenylyl cyclases, with two sets of six transmembrane sequences, each followed by a catalytic domain (C1 and C2). However, amino acids that are enzymatically important and present in the C2 domain of mammalian adenylyl cyclases are located in the C1 domain of the P. falciparum proteins and vice versa. In addition, certain key residues in these domains are more characteristic of guanylyl cyclases. Consistent with this, guanylyl cyclase activity was obtained following expression of the catalytic domains of PfGCbeta in Escherichia coli. In P. falciparum, expression of both genes was detectable in the sexual but not the asexual blood stages of the life cycle, and PfGCalpha was localized to the parasite/parasitophorous vacuole membrane region of gametocytes. The profound structural differences identified between mammalian and parasite guanylyl cyclases suggest that aspects of this signaling pathway may be mechanistically distinct

Single nucleotide variations in ZBTB46 are associated with post-thrombolytic parenchymal haematoma

Haemorrhagic transformation is a complication of recombinant tissue-plasminogen activator treatment. The most severe form, parenchymal haematoma, can result in neurological deterioration, disability, and death. Our objective was to identify single nucleotide variations associated with a risk of parenchymal haematoma following thrombolytic therapy in patients with acute ischaemic stroke. A fixed-effect genome-wide meta-analysis was performed combining two-stage genome-wide association studies (n = 1904). The discovery stage (three cohorts) comprised 1324 ischaemic stroke individuals, 5.4% of whom had a parenchymal haematoma. Genetic variants yielding a P-value < 0.05 1 x 10(-5) were analysed in the validation stage (six cohorts), formed by 580 ischaemic stroke patients with 12.1% haemorrhagic events. All participants received recombinant tissue-plasminogen activator; cases were parenchymal haematoma type 1 or 2 as defined by the European Cooperative Acute Stroke Study (ECASS) criteria. Genome-wide significant findings (P < 5 x 10(-8)) were characterized by in silica functional annotation, gene expression, and DNA regulatory elements. We analysed 7 989 272 single nucleotide polymorphisms and identified a genome-wide association locus on chromosome 20 in the discovery cohort; functional annotation indicated that the ZBTB46 gene was driving the association for chromosome 20. The top single nucleotide polymorphism was rs76484331 in the ZBTB46 gene [P = 2.49 x 10(-8); odds ratio (OR): 11.21; 95% confidence interval (CI): 4.82-26.55]. In the replication cohort (n = 580), the rs76484331 polymorphism was associated with parenchymal haematoma (P = 0.01), and the overall association after meta-analysis increased (P = 1.61 x 10(-8), OR: 5.84; 95% CI: 3.16-10.76). ZBTB46 codes the zinc finger and BTB domain-containing protein 46 that acts as a transcription factor. In silica studies indicated that ZBTB46 is expressed in brain tissue by neurons and endothelial cells. Moreover, rs76484331 interacts with the promoter sites located at 20q13. In conclusion, we identified single nucleotide variants in the ZBTB46 gene associated with a higher risk of parenchymal haematoma following recombinant tissue-plasminogen activator treatment.Peer reviewe

Comparative genomics of a plant-pathogenic fungus, pyrenophora tritici-repentis, reveals transduplication and the impact of repeat elements on pathogenicity and population divergence

Pyrenophora tritici-repentis is a necrotrophic fungus causal to the disease tan spot of wheat, whose contribution to crop loss has increased significantly during the last few decades. Pathogenicity by this fungus is attributed to the production of host-selective toxins (HST), which are recognized by their host in a genotype-specific manner. To better understand the mechanisms that have led to the increase in disease incidence related to this pathogen, we sequenced the genomes of three P. tritici-repentis isolates. A pathogenic isolate that produces two known HSTs was used to assemble a reference nuclear genome of approximately 40 Mb composed of 11 chromosomes that encode 12,141 predicted genes. Comparison of the reference genome with those of a pathogenic isolate that produces a third HST, and a nonpathogenic isolate, showed the nonpathogen genome to be more diverged than those of the two pathogens. Examination of gene-coding regions has provided candidate pathogen-specific proteins and revealed gene families that may play a role in a necrotrophic lifestyle. Analysis of transposable elements suggests that their presence in the genome of pathogenic isolates contributes to the creation of novel genes, effector diversification, possible horizontal gene transfer events, identified copy number variation, and the first example of transduplication by DNA transposable elements in fungi.Overall, comparative analysis of these genomes provides evidence that pathogenicity in this species arose through an influx of transposable elements, which created a genetically flexible landscape that can easily respond to environmental changes

Chlorophyll Meter as Nitrogen Management Tool in Malting Barley

Variable precipitation in many regions makes it difficult to predict yield goals and nitrogen (N) rates for malting grade barley (Hordeum vulgare L.). During years with below normal growing season precipitation, barley fertilized at the recommended rate often exhibits grain protein concentrations exceeding what is acceptable for malting. A study was conducted to evaluate the chlorophyll meter as a N management tool. Barley was grown under several N rates in the field. Chlorophyll meter readings and N additions were made at the Haun 4 to 5 growth stage, and grain yield and protein concentrations were evaluated at maturity. Chlorophyll meter readings, normalized as meter reading from treatment plot divided by that from a plot receiving a full N treatment at the Haun 4 to 5 growth stage, were correlated with grain yield (r2=0.67). Stands having normalized chlorophyll meter readings below 95% responded to N additions with yields equivalent to the fully fertilized stand and grain protein concentrations acceptable for malting. A N management strategy is proposed whereby 40 to 50% of the N calculated for the yield goal is applied at planting and a fully fertilized reference strip is included for each variety or soil type. At the Haun 4 to 5 growth stage, chlorophyll meter readings are taken in the reference strip and in the field. Normalized chlorophyll meter readings below 95% of the reference strip indicate a need for additional N fertilizer. This strategy will provide producers with additional time (up to a month) to evaluate growing season conditions before investing in additional crop inputs and will improve the likelihood that a barley crop acceptable for malting will be produced