Physiological Functions of Skilled and Young Pentathletes at Various Stages of Preparation

Дослідження змагань із різних видів спорту свідчать про те, що велике значення для забезпечення результативної спортивної діяльності мають індивідуально-типологічні властивості нервової системи в спортсменів різної кваліфікації. У статі проаналізовано психофізіологічні функції юних п’ятиборців у різні роки підготовки та кваліфікованих спортсменів із цього виду спорту. Досліджено нейродинамічні особливості спортсменів, котрі займаються сучасним п’ятиборством. Під час різних етапів підготовки спостерігається збільшення мобілізаційних можливостей прояву індивідуально-типологічних характеристик вищої нервової діяльності та зростання когнітивної складової частини функції пам’яті в динаміці підвищення спортивної майстерності. Research of competitions of various sports shows that great importance to ensure the effectiveness of sports activities are individual-typological properties of the nervous system among athletes of different qualifications. The article analyzes physiological functions of skilled and young pentathletes in different years of training. Investigated and analyzed neurodynamic features of athletes who are engaged in modern pentathlon. During various stages of preparation of an increase in manifestations of individual typological characteristics of higher nervous activity and growth of the cognitive component of the memory function in the dynamics of excellence

Predicted bacteriorhodopsin from Exiguobacterium sibiricum is a functional proton pump

AbstractThe predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 534nm without dark adaptation. The ESR photocycle is characterized by fast formation of an M intermediate and the presence of a significant amount of an O intermediate. Proteoliposomes with ESR incorporated transport protons in an outward direction leading to medium acidification. Proton uptake at the cytoplasmic surface of these organelles precedes proton release and coincides with M decay/O rise of the ESR

The site-directed mutation I(L177)H in Rhodobacter sphaeroides reaction center affects coordination of PAP_{A} and BBB_{B} bacteriochlorophylls

AbstractTo explore the influence of the I(L177)H single mutation on the properties of the nearest bacteriochlorophylls (BChls), three reaction centers (RCs) bearing double mutations were constructed in the photosynthetic purple bacterium Rhodobacter sphaeroides, and their properties and pigment content were compared with those of the correspondent single mutant RCs. Each pair of the mutations comprised the amino acid substitution I(L177)H and another mutation altering histidine ligand of BChl PA or BChl BB. Contrary to expectations, the double mutation I(L177)H+H(L173)L does not bring about a heterodimer RC but causes a 46nm blue shift of the long-wavelength P absorbance band. The histidine L177 or a water molecule were suggested as putative ligands for PA in the RC I(L177)H+H(L173)L although this would imply a reorientation of the His backbone and additional rearrangements in the primary donor environment or even a repositioning of the BChl dimer. The crystal structure of the mutant I(L177)H reaction center determined to a resolution of 2.9Å shows changes at the interface region between the BChl PA and the monomeric BChl BB. Spectral and pigment analysis provided evidence for β-coordination of the BChl BB in the double mutant RC I(L177)H+H(M182)L and for its hexacoordination in the mutant reaction center I(L177)H. Computer modeling suggests involvement of two water molecules in the β-coordination of the BChl BB. Possible structural consequences of the L177 mutation affecting the coordination of the two BChls PA and BB are discussed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial