27 research outputs found
A novel method to scale up fungal endophyte isolations
This record contains the Authorâs Accepted Manuscript of an article published in Biocontrol Science and Technology 2015, available online at: http://www.tandfonline.com/ http://dx.doi.org/10.1080/09583157.2015.1033382. Manuscript will be openly accessible in March 2016
Combining methane clumped and bulk isotopes, temporal variations in molecular and isotopic composition, and hydrochemical and geological proxies to understand methaneâs origin in the Ronda peridotite massifs (Spain)
In serpentinised peridotite and ultramafic rock systems, methane (CH4) origin is frequently considered abiotic,
but variable microbial and thermogenic components can also exist. Typically, the origin of CH4 is studied using
bulk, 13C/12C and 2H/H isotopic composition, molecular gas composition, occasionally radiocarbon (14C),
microbiology and geological context. Recent advances in CH4-clumped isotope methods have yielded novel insights
into the formation of CH4: nonetheless, their interpretation in natural gas samples is often uncertain and
requires additional research. Here, we study the origin of the gas released in hyperalkaline (pH > 10) springs in
the Ronda Peridotite Massifs (southern Spain), combining bulk and clumped CH4 isotopes with molecular gas
composition, hydrochemical (Total Organic Carbon and Platinum Group Elements in water), geothermal and
geo-structural data. Five springs analysed in 2014 have been re-examined for changes in gas chemistry over time,
and three newly discovered gas-bearing springs are analysed for the first time. Regardless of whether springs
have microbial or abiotic isotopic fingerprints, we find that bulk CH4 isotopes are fairly stable over a seven-year
period. This suggests that the CH4 source(s) or postgenetic processes (such as oxidation and diffusion) have not
undergone significant temporal changes. Major variations in H2 and CH4 concentrations in certain springs may be
the result of changes in gas pressure and migration intensity. Paired CH4 clumped isotopes (Î12CH2D2 -
Î13CH3D) were analysed in two bubbling springs, where the presence of CH4 can be interpreted as non-microbial
based on 13C enrichment, absence of 14C, and the presence of ethane and propane. However, these isotopes are in
disequilibrium, which prevents the quantification of the gas formation temperature. Within the Î12CH2D2 -
Î13CH3D diagram, the data lie within both the microbialgenic zone, suggested by previous authors, and the
abiotic zone that results combining data from laboratory gas synthesis and other natural gas samples. Therefore,
attributing a microbial origin to CH4 based only on clumped isotopes is less definite than previously assumed.
The amount of Total Organic Carbon appears to be correlated with the origin of CH4, as it is higher in 13Cdepleted
CH4 samples and lower in 13C-enriched samples. Palladium (Pd) and Rhodium (Rh) dissolved in water
(the more soluble Platinum Group Elements) can be a proxy for the chromitite ore deposits contained in
plagioclase tectonite layers throughout the investigated area, which may act as catalysts for abiotic CO2 hydrogenation.
Clumped isotope disequilibrium and the reported absence of diffuse CH4-bearing fluid inclusions in
the peridotites appear to rule out high temperature gas genesis in post-magmatic inclusions. These observations,
along with the moderate temperatures at the base of the peridotite massifs and the consistent occurrence of gasEuropean
Regional Development Fund (FEDER)Junta de AndalucĂaUniversidad de MĂĄlagaFunding charge open
access: University of Malaga / CBU
Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
AbstractWe investigated the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. Both fungal entomopathogens were detected more often in the proximal end of the root than in the distal end. Colonization levels of B. bassiana were higher when plants were sampled at 7â9days post-inoculation (84%) compared to 47â49days post-inoculation (40%). In contrast, the colonization levels of M. anisopliae remained constant from 7â9days post-inoculation (80%) to 47â49days post-inoculation (80%), which suggests M. anisopliae is better able to persist in the soil, or as an endophyte in cassava roots over time. Differences in colonization success and plant growth were found among the fungal entomopathogen treatments
Predictors of Response to Exclusive Enteral Nutrition in Newly Diagnosed CrohnÂŽs Disease in Children: PRESENCE Study from SEGHNP
Exclusive enteral nutrition (EEN) has been shown to be more effective than corticosteroids in achieving mucosal healing in children with CrohnÂŽs disease (CD) without the adverse effects of these drugs. The aims of this study were to determine the efficacy of EEN in terms of inducing clinical remission in children newly diagnosed with CD, to describe the predictive factors of response to EEN and the need for treatment with biological agents during the first 12 months of the disease. We conducted an observational retrospective multicentre study that included paediatric patients newly diagnosed with CD between 2014â2016 who underwent EEN. Two hundred and twenty-two patients (140 males) from 35 paediatric centres were included, with a mean age at diagnosis of 11.6 ± 2.5 years. The median EEN duration was 8 weeks (IQR 6.6â8.5), and 184 of the patients (83%) achieved clinical remission (weighted paediatric Crohnâs Disease activity index [wPCDAI] 15 mg/L and ileal involvement tended to respond better to EEN. EEN administered for 6â8 weeks is effective for inducing clinical remission. Due to the high response rate in our series, EEN should be used as the first-line therapy in luminal paediatric Crohnâs disease regardless of the location of disease and disease activityS
Bones prĂ ctiques en atenciĂł compartida: recomanacions per a una gestiĂł ĂČptima dels PIIC
Bones prĂ ctiques; Presa decisions; Plans dâintervenciĂł individualitzats i compartitsBuenas prĂĄcticas; Toma de decisiones; Planes de intervenciĂłn individualizados i compartidosGood practices; Decision making; Individualized and shared intervention plansAquest document Ă©s un instrument per a professionals de l'atenciĂł sanitĂ ria per posar al dia els criteris de bon Ășs dels PIIC respecte a la revisiĂł que es va publicar lâabril de 2015
State of the climate in 2018
In 2018, the dominant greenhouse gases released into Earthâs atmosphereâcarbon dioxide, methane, and nitrous oxideâcontinued their increase. The annual global average carbon dioxide concentration at Earthâs surface was 407.4 ± 0.1 ppm, the highest in the modern instrumental record and in ice core records dating back 800 000 years. Combined, greenhouse gases and several halogenated gases contribute just over 3 W mâ2 to radiative forcing and represent a nearly 43% increase since 1990. Carbon dioxide is responsible for about 65% of this radiative forcing. With a weak La Niña in early 2018 transitioning to a weak El Niño by the yearâs end, the global surface (land and ocean) temperature was the fourth highest on record, with only 2015 through 2017 being warmer. Several European countries reported record high annual temperatures. There were also more high, and fewer low, temperature extremes than in nearly all of the 68-year extremes record. Madagascar recorded a record daily temperature of 40.5°C in Morondava in March, while South Korea set its record high of 41.0°C in August in Hongcheon. Nawabshah, Pakistan, recorded its highest temperature of 50.2°C, which may be a new daily world record for April. Globally, the annual lower troposphere temperature was third to seventh highest, depending on the dataset analyzed. The lower stratospheric temperature was approximately fifth lowest. The 2018 Arctic land surface temperature was 1.2°C above the 1981â2010 average, tying for third highest in the 118-year record, following 2016 and 2017. Juneâs Arctic snow cover extent was almost half of what it was 35 years ago. Across Greenland, however, regional summer temperatures were generally below or near average. Additionally, a satellite survey of 47 glaciers in Greenland indicated a net increase in area for the first time since records began in 1999. Increasing permafrost temperatures were reported at most observation sites in the Arctic, with the overall increase of 0.1°â0.2°C between 2017 and 2018 being comparable to the highest rate of warming ever observed in the region. On 17 March, Arctic sea ice extent marked the second smallest annual maximum in the 38-year record, larger than only 2017. The minimum extent in 2018 was reached on 19 September and again on 23 September, tying 2008 and 2010 for the sixth lowest extent on record. The 23 September date tied 1997 as the latest sea ice minimum date on record. First-year ice now dominates the ice cover, comprising 77% of the March 2018 ice pack compared to 55% during the 1980s. Because thinner, younger ice is more vulnerable to melting out in summer, this shift in sea ice age has contributed to the decreasing trend in minimum ice extent. Regionally, Bering Sea ice extent was at record lows for almost the entire 2017/18 ice season. For the Antarctic continent as a whole, 2018 was warmer than average. On the highest points of the Antarctic Plateau, the automatic weather station Relay (74°S) broke or tied six monthly temperature records throughout the year, with August breaking its record by nearly 8°C. However, cool conditions in the western Bellingshausen Sea and Amundsen Sea sector contributed to a low melt season overall for 2017/18. High SSTs contributed to low summer sea ice extent in the Ross and Weddell Seas in 2018, underpinning the second lowest Antarctic summer minimum sea ice extent on record. Despite conducive conditions for its formation, the ozone hole at its maximum extent in September was near the 2000â18 mean, likely due to an ongoing slow decline in stratospheric chlorine monoxide concentration. Across the oceans, globally averaged SST decreased slightly since the record El Niño year of 2016 but was still far above the climatological mean. On average, SST is increasing at a rate of 0.10° ± 0.01°C decadeâ1 since 1950. The warming appeared largest in the tropical Indian Ocean and smallest in the North Pacific. The deeper ocean continues to warm year after year. For the seventh consecutive year, global annual mean sea level became the highest in the 26-year record, rising to 81 mm above the 1993 average. As anticipated in a warming climate, the hydrological cycle over the ocean is accelerating: dry regions are becoming drier and wet regions rainier. Closer to the equator, 95 named tropical storms were observed during 2018, well above the 1981â2010 average of 82. Eleven tropical cyclones reached SaffirâSimpson scale Category 5 intensity. North Atlantic Major Hurricane Michaelâs landfall intensity of 140 kt was the fourth strongest for any continental U.S. hurricane landfall in the 168-year record. Michael caused more than 30 fatalities and 6 billion (U.S. dollars) in damages across the Philippines, Hong Kong, Macau, mainland China, Guam, and the Northern Mariana Islands. Tropical Storm Son-Tinh was responsible for 170 fatalities in Vietnam and Laos. Nearly all the islands of Micronesia experienced at least moderate impacts from various tropical cyclones. Across land, many areas around the globe received copious precipitation, notable at different time scales. Rodrigues and RĂ©union Island near southern Africa each reported their third wettest year on record. In Hawaii, 1262 mm precipitation at WaipÄ Gardens (Kauai) on 14â15 April set a new U.S. record for 24-h precipitation. In Brazil, the city of Belo Horizonte received nearly 75 mm of rain in just 20 minutes, nearly half its monthly average. Globally, fire activity during 2018 was the lowest since the start of the record in 1997, with a combined burned area of about 500 million hectares. This reinforced the long-term downward trend in fire emissions driven by changes in land use in frequently burning savannas. However, wildfires burned 3.5 million hectares across the United States, well above the 2000â10 average of 2.7 million hectares. Combined, U.S. wildfire damages for the 2017 and 2018 wildfire seasons exceeded $40 billion (U.S. dollars)
Dissecting the Shared Genetic Architecture of Suicide Attempt, Psychiatric Disorders, and Known Risk Factors
Background Suicide is a leading cause of death worldwide, and nonfatal suicide attempts, which occur far more frequently, are a major source of disability and social and economic burden. Both have substantial genetic etiology, which is partially shared and partially distinct from that of related psychiatric disorders. Methods We conducted a genome-wide association study (GWAS) of 29,782 suicide attempt (SA) cases and 519,961 controls in the International Suicide Genetics Consortium (ISGC). The GWAS of SA was conditioned on psychiatric disorders using GWAS summary statistics via multitrait-based conditional and joint analysis, to remove genetic effects on SA mediated by psychiatric disorders. We investigated the shared and divergent genetic architectures of SA, psychiatric disorders, and other known risk factors. Results Two loci reached genome-wide significance for SA: the major histocompatibility complex and an intergenic locus on chromosome 7, the latter of which remained associated with SA after conditioning on psychiatric disorders and replicated in an independent cohort from the Million Veteran Program. This locus has been implicated in risk-taking behavior, smoking, and insomnia. SA showed strong genetic correlation with psychiatric disorders, particularly major depression, and also with smoking, pain, risk-taking behavior, sleep disturbances, lower educational attainment, reproductive traits, lower socioeconomic status, and poorer general health. After conditioning on psychiatric disorders, the genetic correlations between SA and psychiatric disorders decreased, whereas those with nonpsychiatric traits remained largely unchanged. Conclusions Our results identify a risk locus that contributes more strongly to SA than other phenotypes and suggest a shared underlying biology between SA and known risk factors that is not mediated by psychiatric disorders.Peer reviewe
<scp>ReSurveyEurope</scp>: A database of resurveyed vegetation plots in Europe
AbstractAimsWe introduce ReSurveyEurope â a new data source of resurveyed vegetation plots in Europe, compiled by a collaborative network of vegetation scientists. We describe the scope of this initiative, provide an overview of currently available data, governance, data contribution rules, and accessibility. In addition, we outline further steps, including potential research questions.ResultsReSurveyEurope includes resurveyed vegetation plots from all habitats. Version 1.0 of ReSurveyEurope contains 283,135 observations (i.e., individual surveys of each plot) from 79,190 plots sampled in 449 independent resurvey projects. Of these, 62,139 (78%) are permanent plots, that is, marked in situ, or located with GPS, which allow for high spatial accuracy in resurvey. The remaining 17,051 (22%) plots are from studies in which plots from the initial survey could not be exactly relocated. Four data sets, which together account for 28,470 (36%) plots, provide only presence/absence information on plant species, while the remaining 50,720 (64%) plots contain abundance information (e.g., percentage cover or coverâabundance classes such as variants of the BraunâBlanquet scale). The oldest plots were sampled in 1911 in the Swiss Alps, while most plots were sampled between 1950 and 2020.ConclusionsReSurveyEurope is a new resource to address a wide range of research questions on fineâscale changes in European vegetation. The initiative is devoted to an inclusive and transparent governance and data usage approach, based on slightly adapted rules of the wellâestablished European Vegetation Archive (EVA). ReSurveyEurope data are ready for use, and proposals for analyses of the data set can be submitted at any time to the coordinators. Still, further data contributions are highly welcome.</jats:sec
Enhancing Microbiome Research through Genome-Scale Metabolic Modeling
Construction and analysis of genome-scale metabolic models (GEMs) is a well-established systems biology approach that can be used to predict metabolic and growth phenotypes. The ability of GEMs to produce mechanistic insight into microbial ecological processes makes them appealing tools that can open a range of exciting opportunities in microbiome research. Here, we briefly outline these opportunities, present current rate-limiting challenges for the trustworthy application of GEMs to microbiome research, and suggest approaches for moving the field forward.publishedVersio