The living dead : a design for a cemetery in New Orleans

Though this thesis began as an investigation of how a kit-of-parts might be conceived and applied to the malting of an urban cemetery, the actual design process for the cemetery required jettisoning the kit-of-paids approach in favor of a more responsive and unique design methodology. This second approach turned out to be the more appropriate means of forming a design solution for the site hounded by Royal Street, Piety Street, Desire Street, and Chartres Street and a similar design methodology would likely have been deployed at the other two sites as well. In the end, making explicit and implicit references to New Orleans\u27 funereal and cultural heritages in the design for the cemetery hopefully revealed a poetic, enduring and functional vision of a Cemetery in an urban setting that is also a gathering Place for the living inextricably United to its surroundings

Learning outcomes of a narrative exchange program for high school students : empathy and related constructs

Narrative 4 is an organization of writers, artists, teachers, and other community leaders, which isprimarily focused on promoting empathy and prosocial behavior among high school students.Narrative 4 uses a unique narrative exchange process and curriculum as their method foraccomplishing this goal. To the author’s knowledge, this is the first study to systematicallyinvestigate the effectiveness of this unique program in promoting participant empathy andprosocial behavior. Analysis was limited due to low participation (N=13) and incomplete data.Pretest and posttest measures of empathy and related constructs were taken before and afterparticipation in the program. It was predicted that posttest measures of emotional contagion,cognitive empathy, empathic concern, perspective-taking, and prosocial behavior would besignificantly higher than pretest measures. Paired-sample t-tests were used to examine the datafor significant differences. Contrary to prediction, the only significant change was a decrease incognitive empathy. However, this change was seen only after eliminating a participant’s pair ofoutlier scores to meet normal distribution assumptions for analysis, and caution is recommendedin interpreting the result. It was also hypothesized that changes in prosocial behavior would bemediated by changes in affective empathy (emotional contagion). Because no significantdifference was found between pretest and posttest measures, mediation analysis was not performed. Relationships of changes in empathy measures were also examined using Pearson’sproduct-moment correlation values. It was hypothesized that changes in cognitive empathy andperspective-taking would negatively correlate with changes in emotional disconnection andpersonal distress. Results were unable to provide support for this hypothesis, as the statedrelationships between difference scores were not found to be significant. Lastly, it was predictedthat students’ written reflections on the N4 program would reveal mostly positive viewsregarding the experience, as well as themes of community bonding. Only one participant forwhom consent and assent was obtained provided a reflection. Therefore, this prediction was notevaluated. Discussion follows, including that of the challenges of conducting research withinschools, limitations of the study, and suggested future directions for research

Genetic and phenotypic diversity in Burkholderia: contributions by prophage and phage-like elements

<p>Abstract</p> <p>Background</p> <p><it>Burkholderia </it>species exhibit enormous phenotypic diversity, ranging from the nonpathogenic, soil- and water-inhabiting <it>Burkholderia thailandensis </it>to the virulent, host-adapted mammalian pathogen <it>B. mallei</it>. Genomic diversity is evident within <it>Burkholderia </it>species as well. Individual isolates of <it>Burkholderia pseudomallei </it>and <it>B. thailandensis</it>, for example, carry a variety of strain-specific genomic islands (GIs), including putative pathogenicity and metabolic islands, prophage-like islands, and prophages. These GIs may provide some strains with a competitive advantage in the environment and/or in the host relative to other strains.</p> <p>Results</p> <p>Here we present the results of analysis of 37 prophages, putative prophages, and prophage-like elements from six different <it>Burkholderia </it>species. Five of these were spontaneously induced to form bacteriophage particles from <it>B. pseudomallei </it>and <it>B. thailandensis </it>strains and were isolated and fully sequenced; 24 were computationally predicted in sequenced <it>Burkholderia </it>genomes; and eight are previously characterized prophages or prophage-like elements. The results reveal numerous differences in both genome structure and gene content among elements derived from different species as well as from strains within species, due in part to the incorporation of additional DNA, or 'morons' into the prophage genomes. Implications for pathogenicity are also discussed. Lastly, RNAseq analysis of gene expression showed that many of the genes in ϕ1026b that appear to contribute to phage and lysogen fitness were expressed independently of the phage structural and replication genes.</p> <p>Conclusions</p> <p>This study provides the first estimate of the relative contribution of prophages to the vast phenotypic diversity found among the <it>Burkholderiae</it>.</p

Transcriptomic analysis of cutaneous squamous cell carcinoma reveals a multi-gene prognostic signature associated with metastasis

Background: Metastasis of cutaneous squamous cell carcinoma (cSCC) is uncommon. Current staging methods are reported to have sub-optimal performances in metastasis prediction. Accurate identification of patients with tumours at high risk of metastasis would have a significant impact on management.Objective: To develop a robust and validated gene expression profile (GEP) signature for predicting primary cSCC metastatic risk using an unbiased whole transcriptome discovery-driven approach.Methods: Archival formalin-fixed paraffin-embedded primary cSCC with perilesional normal tissue from 237 immunocompetent patients (151 non-metastasising and 86 metastasising) were collected retrospectively from four centres. TempO-seq was used to probe the whole transcriptome and machine learning algorithms were applied to derive predictive signatures, with a 3:1 split for training and testing datasets.Results: A 20-gene prognostic model was developed and validated, with an accuracy of 86.0%, sensitivity of 85.7%, specificity of 86.1%, and positive predictive value of 78.3% in the testing set, providing more stable, accurate prediction than pathological staging systems. A linear predictor was also developed, significantly correlating with metastatic risk.Limitations: This was a retrospective 4-centre study and larger prospective multicentre studies are now required.Conclusion: The 20-gene signature prediction is accurate, with the potential to be incorporated into clinical workflows for cSCC

Observations of the anisotropy in the cosmic microwave background by the FIRS, SK93, and MSAM-I experiments

The observations and results from the FIRS, SK93, and MSAM-1, experiments are discussed. These experiments search for anisotropy in the cosmic microwave background over a range in angular scale from 180 deg to 0.5 deg and a range in frequency from 26 to 680 GHz. Emphasis is placed on the observing strategy and potential systematic errors. Contamination of the data by galactic sources is addressed. Future directions are indicated. The results for all three experiments, as found by us and others, are given in the context of the standard CDM model, Q(sub CDM), and the model-independent band-power estimates

Rationale and design of the Exercise Intensity Trial (EXCITE): A randomized trial comparing the effects of moderate versus moderate to high-intensity aerobic training in women with operable breast cancer

<p>Abstract</p> <p>Background</p> <p>The Exercise Intensity Trial (EXcITe) is a randomized trial to compare the efficacy of supervised moderate-intensity aerobic training to moderate to high-intensity aerobic training, relative to attention control, on aerobic capacity, physiologic mechanisms, patient-reported outcomes, and biomarkers in women with operable breast cancer following the completion of definitive adjuvant therapy.</p> <p>Methods/Design</p> <p>Using a single-center, randomized design, 174 postmenopausal women (58 patients/study arm) with histologically confirmed, operable breast cancer presenting to Duke University Medical Center (DUMC) will be enrolled in this trial following completion of primary therapy (including surgery, radiation therapy, and chemotherapy). After baseline assessments, eligible participants will be randomized to one of two supervised aerobic training interventions (moderate-intensity or moderate/high-intensity aerobic training) or an attention-control group (progressive stretching). The aerobic training interventions will include 150 mins.wk^-1of supervised treadmill walking per week at an intensity of 60%-70% (moderate-intensity) or 60% to 100% (moderate to high-intensity) of the individually determined peak oxygen consumption (VO_2peak) between 20-45 minutes/session for 16 weeks. The progressive stretching program will be consistent with the exercise interventions in terms of program length (16 weeks), social interaction (participants will receive one-on-one instruction), and duration (20-45 mins/session). The primary study endpoint is VO_2peak, as measured by an incremental cardiopulmonary exercise test. Secondary endpoints include physiologic determinants that govern VO_2peak, patient-reported outcomes, and biomarkers associated with breast cancer recurrence/mortality. All endpoints will be assessed at baseline and after the intervention (16 weeks).</p> <p>Discussion</p> <p>EXCITE is designed to investigate the intensity of aerobic training required to induce optimal improvements in VO_2peakand other pertinent outcomes in women who have completed definitive adjuvant therapy for operable breast cancer. Overall, this trial will inform and refine exercise guidelines to optimize recovery in breast and other cancer survivors following the completion of primary cytotoxic therapy.</p> <p>Trial Registration</p> <p>NCT01186367</p

Piezoelectric polymer composites for sensors and actuators

As a result of the Internet of Things (IoT) and Industry 4.0 paradigms, based on increasing interconnectivity, the development of advanced high-performance materials for sensor and actuator applications are increasingly required. In particular, piezoelectric composites are of large scientific and technological interest from fundamental and applied point of views. Piezoelectric composites are applied in a wide range of applications as they combine the excellent properties of polymers and ceramics. The definition and properties of piezoelectric materials and composites are presented as well as the recent applications in areas such as electronics, energy harvesting, environmental sensors and biomedical applications. The outlook and future trends for piezoelectric composites are also provided.FCT (Fundação para a Ciência e Tecnologia) for financial support under the framework of Strategic Funding grants UID/FIS/04650/2020, UID/EEA/04436/2020 and UID/QUI/0686/2020; and project no. PTDC/FISMAC/28157/2017, PTDC/BTM-MAT/28237/2017 and PTDC/EMDEMD/28159/2017. The authors also thank the FCT for financial support under grants SFRH/BD/145455/2019 (E.C.), SFRH/BD/145345/2019 (L.F.) and SFRH/BPD/112547/2015 (C.M.C.). Financial support from the Basque Government Industry and Education Departments under the ELKARTEK, HAZITEK and PIBA (PIBA-2018-06

Pathema: a clade-specific bioinformatics resource center for pathogen research

Pathema (http://pathema.jcvi.org) is one of the eight Bioinformatics Resource Centers (BRCs) funded by the National Institute of Allergy and Infectious Disease (NIAID) designed to serve as a core resource for the bio-defense and infectious disease research community. Pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target NIAID Category A–C pathogens: Category A priority pathogens; Bacillus anthracis and Clostridium botulinum, and Category B priority pathogens; Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens and Entamoeba histolytica. Each target pathogen is represented in one of four distinct clade-specific Pathema web resources and underlying databases developed to target the specific data and analysis needs of each scientific community. All publicly available complete genome projects of phylogenetically related organisms are also represented, providing a comprehensive collection of organisms for comparative analyses. Pathema facilitates the scientific exploration of genomic and related data through its integration with web-based analysis tools, customized to obtain, display, and compute results relevant to ongoing pathogen research. Pathema serves the bio-defense and infectious disease research community by disseminating data resulting from pathogen genome sequencing projects and providing access to the results of inter-genomic comparisons for these organisms

Follicular Dendritic Cell-Specific Prion Protein (PrPc) Expression Alone Is Sufficient to Sustain Prion Infection in the Spleen

Prion diseases are characterised by the accumulation of PrPSc, an abnormally folded isoform of the cellular prion protein (PrPC), in affected tissues. Following peripheral exposure high levels of prion-specific PrPSc accumulate first upon follicular dendritic cells (FDC) in lymphoid tissues before spreading to the CNS. Expression of PrPC is mandatory for cells to sustain prion infection and FDC appear to express high levels. However, whether FDC actively replicate prions or simply acquire them from other infected cells is uncertain. In the attempts to-date to establish the role of FDC in prion pathogenesis it was not possible to dissociate the Prnp expression of FDC from that of the nervous system and all other non-haematopoietic lineages. This is important as FDC may simply acquire prions after synthesis by other infected cells. To establish the role of FDC in prion pathogenesis transgenic mice were created in which PrPC expression was specifically “switched on” or “off” only on FDC. We show that PrPC-expression only on FDC is sufficient to sustain prion replication in the spleen. Furthermore, prion replication is blocked in the spleen when PrPC-expression is specifically ablated only on FDC. These data definitively demonstrate that FDC are the essential sites of prion replication in lymphoid tissues. The demonstration that Prnp-ablation only on FDC blocked splenic prion accumulation without apparent consequences for FDC status represents a novel opportunity to prevent neuroinvasion by modulation of PrPC expression on FDC