In situ induction of dendritic cell–based T cell tolerance in humanized mice and nonhuman primates

Administration of an ICAM-1–specific antibody arrests dendritic cells in a semi-immature state and facilitates antigen-specific T cell tolerance to islet allografts in humanized mice and Rhesus monkeys

The Consumption of Wild Edible Plants

Wild edible plants are of great importance in both former and current human societies. Their use embodies evolutionary trends, continuing interactions between men and nature, relevant traditional knowledge, and cultural heritage. A conceptual approach to wild edible plants, including the contribution of such species to people's diets and daily lives, focusing on nutritional and cultural value, food sovereignty and security, as well as the huge legacy for future generations, leads to a general overview of new tendencies and availability of wild plant resources according to geographic regions. The potential benefits and the continual need for conservation strategies of plants, habitats, and associated knowledge are also discussed, enhancing biodiversity and biocultural patrimony. Finally, some examples of culturally significant edible wild plants are described, emphasizing the importance of knowledge transmission and sustainable uses in a changing world.info:eu-repo/semantics/publishedVersio

GAL4 directs nucleosome sliding induced by NURF

The Drosophila nucleosome remodeling factor (NURF) is an imitation switch (ISWI)-containing chromatin remodeling complex that can catalyze nucleosome repositioning at promoter regions to regulate access by the transcription machinery. Mononucleosomes reconstituted in vitro by salt dialysis adopt an ensemble of translational positions on DNA templates. NURF induces bi-directional ‘sliding’ of these nucleosomes to a subset of preferred positions. Here we show that mononucleosome sliding catalyzed by NURF bears similarity to nucleosome movement induced by elevated temperature. Moreover, we demonstrate that the GAL4 DNA-binding domain can extend NURF-induced nucleosome movement on a GAL4-E4 promoter, expanding the stretch of histone-free DNA at GAL4 recognition sites. The direction of NURF-induced nucleosome movement can be significantly modulated by asymmetric placement of tandem GAL4 sites relative to the nucleosome core particle. As such, sequence-specific, transcription factor-directed nucleosome sliding is likely to have substantial influence on promoter activation