Secretion of Novel SEL1L Endogenous Variants Is Promoted by ER Stress/UPR via Endosomes and Shed Vesicles in Human Cancer Cells

We describe here two novel endogenous variants of the human endoplasmic reticulum (ER) cargo receptor SEL1LA, designated p38 and p28. Biochemical and RNA interference studies in tumorigenic and non-tumorigenic cells indicate that p38 and p28 are N-terminal, ER-anchorless and more stable relative to the canonical transmembrane SEL1LA. P38 is expressed and constitutively secreted, with increase after ER stress, in the KMS11 myeloma line and in the breast cancer lines MCF7 and SKBr3, but not in the non-tumorigenic breast epithelial MCF10A line. P28 is detected only in the poorly differentiated SKBr3 cell line, where it is secreted after ER stress. Consistently with the presence of p38 and p28 in culture media, morphological studies of SKBr3 and KMS11 cells detect N-terminal SEL1L immunolabeling in secretory/degradative compartments and extracellularly-released membrane vesicles. Our findings suggest that the two new SEL1L variants are engaged in endosomal trafficking and secretion via vesicles, which could contribute to relieve ER stress in tumorigenic cells. P38 and p28 could therefore be relevant as diagnostic markers and/or therapeutic targets in cancer

Measurement of the production cross-section of psi(2S) -> J/psi(-> mu(+)mu(-))pi(+) pi(-) in pp collisions at root s=7 TeV at ATLAS

© 2014, The Author(s). Abstract: The prompt and non-prompt production cross-sections for ψ(2S) mesons are measured using 2.1 fb−1 of pp collision data at a centre-of-mass energy of 7 TeV recorded by the ATLAS experiment at the LHC. The measurement exploits the ψ(2S) → J/ψ(→ μ+μ−)π+π− decay mode, and probes ψ(2S) mesons with transverse momenta in the range 10 ≤ pT< 100 GeV and rapidity |y| < 2.0. The results are compared to other measurements of ψ(2S) production at the LHC and to various theoretical models for prompt and non-prompt quarkonium production.[Figure not available: see fulltext.]

Measurement of the production cross-section of ψ(2S) → [J over ψ](→μ[superscript +] μ[superscript −]) π[superscript +] π [superscript −] in pp collisions at √s = 7 TeV at ATLAS

The prompt and non-prompt production cross-sections for ψ(2S) mesons are measured using 2.1 fb[superscript −1] of pp collision data at a centre-of-mass energy of 7 TeV recorded by the ATLAS experiment at the LHC. The measurement exploits the ψ(2S) → [J over ψ](→ μ[superscript +] μ[superscript −])π[superscript +] π[superscript −] decay mode, and probes ψ(2S) mesons with transverse momenta in the range 10 ≤ p[subscript T] < 100 GeV and rapidity |y| < 2.0. The results are compared to other measurements of ψ(2S) production at the LHC and to various theoretical models for prompt and non-prompt quarkonium production.European Organization for Nuclear ResearchUnited States. Dept. of EnergyNational Science Foundation (U.S.)Brookhaven National Laborator