269 research outputs found
UV Light Shower Simulator for Fluorescence and Cerenkov Radiation Studies
All experiments observing showers light use telescopes equipped with
pixellised photodetectors. Monte-Carlo (MC) simulations of the apparatus
operation in various situations (background light, shower energy, proximity of
tracks...) are mandatory, but never enter into detector details like pulse
shape, dead-time, or charge space effects which are finally responsible for the
data quality. An apparatus where each pixel receives light from individual 370
nm UV LEDs through silica fibers is being built. The LEDs receive voltage
through DACs, which get their input (which pixel, at what time, which
amplitude) from a shower plus noise generator code. The typical time constant
of a shower being one s (300 m for light), the pulses are one s wide.
This is rather long compared to the intrinsic time constant (around 10 ns) of
the light detectors, hence, these see "constant light" changing every s.
This is where important loading effects which are not included in MC code can
be observed. The fibers illuminate the pixels through a diffuser, and each
fiber illuminates only one pixel. The number of equipped pixels is such that it
englobes a full shower (much less than the full focal surface). Finally, this
equipment can be used also to calibrate the pixels
MSACompro: protein multiple sequence alignment using predicted secondary structure, solvent accessibility, and residue-residue contacts
<p>Abstract</p> <p>Background</p> <p>Multiple Sequence Alignment (MSA) is a basic tool for bioinformatics research and analysis. It has been used essentially in almost all bioinformatics tasks such as protein structure modeling, gene and protein function prediction, DNA motif recognition, and phylogenetic analysis. Therefore, improving the accuracy of multiple sequence alignment is important for advancing many bioinformatics fields.</p> <p>Results</p> <p>We designed and developed a new method, MSACompro, to synergistically incorporate predicted secondary structure, relative solvent accessibility, and residue-residue contact information into the currently most accurate posterior probability-based MSA methods to improve the accuracy of multiple sequence alignments. The method is different from the multiple sequence alignment methods (e.g. 3D-Coffee) that use the tertiary structure information of some sequences since the structural information of our method is fully predicted from sequences. To the best of our knowledge, applying predicted relative solvent accessibility and contact map to multiple sequence alignment is novel. The rigorous benchmarking of our method to the standard benchmarks (i.e. BAliBASE, SABmark and OXBENCH) clearly demonstrated that incorporating predicted protein structural information improves the multiple sequence alignment accuracy over the leading multiple protein sequence alignment tools without using this information, such as MSAProbs, ProbCons, Probalign, T-coffee, MAFFT and MUSCLE. And the performance of the method is comparable to the state-of-the-art method PROMALS of using structural features and additional homologous sequences by slightly lower scores.</p> <p>Conclusion</p> <p>MSACompro is an efficient and reliable multiple protein sequence alignment tool that can effectively incorporate predicted protein structural information into multiple sequence alignment. The software is available at <url>http://sysbio.rnet.missouri.edu/multicom_toolbox/</url>.</p
APC/CCdh1-Mediated Degradation of the F-Box Protein NIPA Is Regulated by Its Association with Skp1
NIPA (Nuclear Interaction Partner of Alk kinase) is an F-box like protein
that targets nuclear Cyclin B1 for degradation. Integrity and therefore activity
of the SCFNIPA E3 ligase is regulated by cell-cycle-dependent phosphorylation
of NIPA, restricting substrate ubiquitination to interphase. Here we show
that phosphorylated NIPA is degraded in late mitosis in an APC/CCdh1-dependent
manner. Binding of the unphosphorylated form of NIPA to Skp1 interferes with
binding to the APC/C-adaptor protein Cdh1 and therefore protects unphosphorylated
NIPA from degradation in interphase. Our data thus define a novel mode of
regulating APC/C-mediated ubiquitination
Colour categories are reflected in sensory stages of colour perception when stimulus issues are resolved
Debate exists about the time course of the effect of colour categories on visual processing. We investigated the effect of colour categories for two groups who differed in whether they categorised a blue-green boundary colour as the same- or different-category to a reliably-named blue colour and a reliably-named green colour. Colour differences were equated in just-noticeable differences to be equally discriminable. We analysed event-related potentials for these colours elicited on a passive visual oddball task and investigated the time course of categorical effects on colour processing. Support for category effects was found 100 ms after stimulus onset, and over frontal sites around 250 ms, suggesting that colour naming affects both early sensory and later stages of chromatic processing
Design concepts for the Cherenkov Telescope Array CTA: an advanced facility for ground-based high-energy gamma-ray astronomy
Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5-10 improvement in sensitivity in the 100 GeV-10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA
Letter of intent for KM3NeT 2.0
The main objectives of the KM3NeT Collaboration are
(
i
)
the discovery and
subsequent observation of high-energy neutrino sources in the Universe and
(
ii
)
the determination of the mass hierarchy of neutrinos. These objectives are
strongly motivated by two recent important discoveries, namely:
(
1
)
the high-
energy astrophysical neutrino signal reported by IceCube and
(
2
)
the sizable
contribution of electron neutrinos to the third neutrino mass eigenstate as
reported by Daya Bay, Reno and others. To meet these objectives, the
KM3NeT Collaboration plans to build a new Research Infrastructure con-
sisting of a network of deep-sea neutrino telescopes in the Mediterranean Sea.
A phased and distributed implementation is pursued which maximises the
access to regional funds, the availability of human resources and the syner-
gistic opportunities for the Earth and sea sciences community. Three suitable
deep-sea sites are selected, namely off-shore Toulon
(
France
)
, Capo Passero
(
Sicily, Italy
)
and Pylos
(
Peloponnese, Greece
)
. The infrastructure will consist
of three so-called building blocks. A building block comprises 115 strings,
each string comprises 18 optical modules and each optical module comprises
31 photo-multiplier tubes. Each building block thus constitutes a three-
dimensional array of photo sensors that can be used to detect the Cherenkov
light produced by relativistic particles emerging from neutrino interactions.
Two building blocks will be sparsely con
fi
gured to fully explore the IceCube
signal with similar instrumented volume, different methodology, improved
resolution and complementary
fi
eld of view, including the galactic plane. One
building block will be densely con
fi
gured to precisely measure atmospheric
neutrino oscillations.
Original content from this work may be used under the ter
Southern African Large Telescope Spectroscopy of BL Lacs for the CTA project
In the last two decades, very-high-energy gamma-ray astronomy has reached maturity: over 200 sources have been detected, both Galactic and extragalactic, by ground-based experiments. At present, Active Galactic Nuclei (AGN) make up about 40% of the more than 200 sources detected at very high energies with ground-based telescopes, the majority of which are blazars, i.e. their jets are closely aligned with the line of sight to Earth and three quarters of which are classified as high-frequency peaked BL Lac objects. One challenge to studies of the cosmological evolution of BL Lacs is the difficulty of obtaining redshifts from their nearly featureless, continuum-dominated spectra. It is expected that a significant fraction of the AGN to be detected with the future Cherenkov Telescope Array (CTA) observatory will have no spectroscopic redshifts, compromising the reliability of BL Lac population studies, particularly of their cosmic evolution. We started an effort in 2019 to measure the redshifts of a large fraction of the AGN that are likely to be detected with CTA, using the Southern African Large Telescope (SALT). In this contribution, we present two results from an on-going SALT program focused on the determination of BL Lac object redshifts that will be relevant for the CTA observatory
- …