Toward Improved Understanding of Changes in Greenland Outlet Glacier Shear Margin Dynamics in a Warming Climate

The Greenland Ice Sheet has experienced accelerated mass loss over the last couple decades, in part due to destabilization of marine-terminating outlet glaciers. Retreat and acceleration of outlet glaciers coincides with atmospheric and oceanic warming resulting in a significant contribution to sea-level rise. The relative role of surface meltwater production, runoff and infiltration on the dynamics of these systems is not well-understood. To assess how surface meltwater impacts shear margin dynamics and regional ice flow of outlet glaciers, we investigate the impact of basal lubrication of Jakobshavn Isbræ shear margins due to drainage from water-filled crevasses. We map the areal extent of inundated crevasses during summer (May–August) from 2000 to 2012 using satellite imagery and determined an increasing trend in the total areal extent over this time interval. We use a numerical ice flow model to quantify the potential impact of weakened shear margins due to surface melt derived basal lubrication on regional flow velocities. Ice flow velocities 10 km from the lateral margins of Jakobshavn were amplified by as much as 20%, resulting in an increase of ~0.6 Gt yr−1 in ice-mass discharge through the shear margins into the ice stream. Under future warming scenarios with increased surface melt ponding, simulations indicate up to a 30% increase in extra-marginal ice flow. We conclude that surface meltwater will likely play an important role in the evolving dynamics of glacier shear margins and the future mass flux through Greenland's major marine-terminating outlet glaciers

Greenland ice sheet annual motion insensitive to spatial variations in subglacial hydraulic structure

We present ice velocities observed with global positioning systems and TerraSAR-X/TanDEM-Xin a land-terminating region of the southwest Greenland ice sheet (GrIS) during the melt year 2012–2013, toexamine the spatial pattern of seasonal and annual ice motion. We find that while spatial variability in theconfiguration of the subglacial drainage system controls ice motion at short timescales, this configurationhas negligible impact on the spatial pattern of the proportion of annual motion which occurs duringsummer. While absolute annual velocities vary substantially, the proportional contribution of summermotion to annual motion does not. These observations suggest that in land-terminating margins of the GrIS,subglacial hydrology does not significantly influence spatial variations in net summer speedup.Furthermore, our findings imply that not every feature of the subglacial drainage system needs to beresolved in ice sheet models

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications

We have designed a novel non-antibody scaffold protein, termed Adhiron, based on a phytocystatin consensus sequence. The Adhiron scaffold shows high thermal stability (Tm ca. 101°C), and is expressed well in Escherichia coli. We have determined the X-ray crystal structure of the Adhiron scaffold to 1.75 Å resolution revealing a compact cystatin-like fold. We have constructed a phage-display library in this scaffold by insertion of two variable peptide regions. The library is of high quality and complexity comprising 1.3 × 10(10) clones. To demonstrate library efficacy, we screened against the yeast Small Ubiquitin-like Modifier (SUMO). In selected clones, variable region 1 often contained sequences homologous to the known SUMO interactive motif (V/I-X-V/I-V/I). Four Adhirons were further characterised and displayed low nanomolar affinities and high specificity for yeast SUMO with essentially no cross-reactivity to human SUMO protein isoforms. We have identified binders against >100 target molecules to date including as examples, a fibroblast growth factor (FGF1), platelet endothelial cell adhesion molecule (PECAM-1; CD31), the SH2 domain Grb2 and a 12-aa peptide. Adhirons are highly stable and well expressed allowing highly specific binding reagents to be selected for use in molecular recognition applications

Seasonal speedup of a Greenland marine-terminating outlet glacier forced by surface melt–induced changes in subglacial hydrology

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Evidence of an active volcanic heat source beneath the Pine Island Glacier

Tectonic landforms reveal that the West Antarctic Ice Sheet (WAIS) lies atop a major volcanic rift system. However, identifying subglacial volcanism is challenging. Here we show geochemical evidence of a volcanic heat source upstream of the fast-melting Pine Island Ice Shelf, documented by seawater helium isotope ratios at the front of the Ice Shelf cavity. The localization of mantle helium to glacial meltwater reveals that volcanic heat induces melt beneath the grounded glacier and feeds the subglacial hydrological network crossing the grounding line. The observed transport of mantle helium out of the Ice Shelf cavity indicates that volcanic heat is supplied to the grounded glacier at a rate of ~ 2500 ± 1700 MW, which is ca. half as large as the active Grimsvötn volcano on Iceland. Our finding of a substantial volcanic heat source beneath a major WAIS glacier highlights the need to understand subglacial volcanism, its hydrologic interaction with the marine margins, and its potential role in the future stability of the WAIS

Sub-lethal concentrations of CdCl2 disrupt cell migration and cytoskeletal proteins in cultured mouse TM4 Sertoli cells

The aims of this study were to examine the effects of CdCl2 on the viability, migration and cytoskeleton of cultured mouse TM4 Sertoli cells. Time- and concentration-dependent changes were exhibited by the cells but 1 µM CdCl2 was sub-cytotoxic at all time-points. Exposure to 1 and 12 µM CdCl2 for 4 h resulted in disruption of the leading edge, as determined by chemical staining. Cell migration was inhibited by both 1 and 12 µM CdCl2 in a scratch assay monitored by live cell imaging, although exposure to the higher concentration was associated with cell death. Western blotting and immunofluorescence staining indicated that CdCl2 caused a concentration dependent reduction in actin and tubulin levels. Exposure to Cd2+ also resulted in significant changes in the levels and/or phosphorylation status of the microtubule and microfilament destabilising proteins cofilin and stathmin, suggesting disruption of cytoskeletal dynamics. Given that 1-12 µM Cd2+ is attainable in vivo, our findings are consistent with the possibility that Cd2+ induced impairment of testicular development and reproductive health may involve a combination of reduced Sertoli cell migration and impaired Sertoli cell viability depending on the timing, level and duration of exposure

Primary rat sertoli and interstitial cells exhibit a differential response to cadmium

Two cell types central to the support of spermatogenesis, the Sertoli cell and the interstitial (Leydig) cell, were isolated from the same cohort of young male rats and challenged with cadmium chloride to compare their susceptibility to the metal. Both cell types were cultured under similar conditions, and similar biochemical endpoints were chosen to minimize experimental variability. These endpoints include the uptake of 109 Cd, reduction of the vital tetrazolium dye MTT, incorporation of 3 H-leucine, change in heat-stable cadmium binding capacity, and production of lactate. Using these parameters, it was observed that the Sertoli cell cultures were adversely affected in a dose-and time-dependent manner, while the interstitial cell cultures, treated with identical concentrations of CdCl 2 , were less affected. The 72-hr LC 50 's for Sertoli cells and interstitial cells were 4.1 and 19.6 μM CdCl 2 , respectively. Thus, different cell populations within the same tissue may differ markedly in susceptibility to a toxicant. These in vitro data suggest that the Sertoli cell, in relation to the interstitium, is particularly sensitive to cadmium. Because the Sertoli cell provides functional support for the seminiferous epithelium, the differential sensitivity of this cell type may, in part, explain cadmium-induced testicular dysfunction, particularly at doses that leave the vascular epithelium intact.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42554/1/10565_2004_Article_BF00135027.pd