225 research outputs found
Shell-structure effects on high-pressure Rankine-Hugoniot shock adiabats
Rankine-Hugoniot shock adiabats are calculated in the pressure range 1
Mbar-10 Gbar with two atomic-structure models: the atom in a spherical cell and
the atom in a jellium of charges. These quantum self-consistent-field models
include shell effects, which have a strong impact on pressure and shock
velocity along the shock adiabat. Comparisons with experimental data are
presented and quantum effects are interpreted in terms of electronic specific
heat. A simple analytical estimate for the maximum compression is proposed,
depending on initial density, atomic weight and atomic number
Spin-orbit Scattering and the Kondo Effect
The effects of spin-orbit scattering of conduction electrons in the Kondo
regime are investigated theoretically. It is shown that due to time-reversal
symmetry, spin-orbit scattering does not suppress the Kondo effect, even though
it breaks spin-rotational symmetry, in full agreement with experiment. An
orbital magnetic field, which breaks time-reversal symmetry, leads to an
effective Zeeman splitting, which can be probed in transport measurements. It
is shown that, similar to weak-localization, this effect has anomalous magnetic
field and temperature dependence.Comment: 10 pages, RevTex, one postscript figure available on request from
[email protected]
Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous gene's polyadenylation sequence and provide transcript stability. However, the spectrum of mutations generated by these vectors appears largely restricted to the last intron of target loci due to nonsense-mediated mRNA decay (NMD) making them unsuitable for gene targeting applications. Here, we present novel poly A trap vectors that overcome the effect of NMD and also employ RNA instability sequences to improve splicing efficiency. The set of random insertions generated with these vectors show a significantly reduced insertional bias and the vectors can be targeted directly to a 5′ intron. We also show that this relative positional independence is linked to the human β-actin promoter and is most likely a result of its transcriptional activity in ES cells. Taken together our data indicate that these vectors are an effective tool for insertional mutagenesis that can be used for either gene trapping or gene targeting
A Fermi Surface study of BaKBiO
We present all electron computations of the 3D Fermi surfaces (FS's) in
BaKBiO for a number of different compositions based on the
selfconsistent Korringa-Kohn-Rostoker coherent-potential-approximation
(KKR-CPA) approach for incorporating the effects of Ba/K substitution. By
assuming a simple cubic structure throughout the composition range, the
evolution of the nesting and other features of the FS of the underlying
pristine phase is correlated with the onset of various structural transitions
with K doping. A parameterized scheme for obtaining an accurate 3D map of the
FS in BaKBiO for an arbitrary doping level is developed. We
remark on the puzzling differences between the phase diagrams of
BaKBiO and BaPbBiO by comparing aspects
of their electronic structures and those of the end compounds BaBiO,
KBiO and BaPbO. Our theoretically predicted FS's in the cubic phase are
relevant for analyzing high-resolution Compton scattering and
positron-annihilation experiments sensitive to the electron momentum density,
and are thus amenable to substantial experimental verification.Comment: 12 pages, 7 figures, to appear in Phys. Rev.
First principles simulations of liquid Fe-S under Earth's core conditions
First principles electronic structure calculations, based upon density
functional theory within the generalized gradient approximation and ultra-soft
Vanderbilt pseudopotentials, have been used to simulate a liquid alloy of iron
and sulfur at Earth's core conditions. We have used a sulfur concentration of
wt, in line with the maximum recent estimates of the sulfur
abundance in the Earth's outer core. The analysis of the structural, dynamical
and electronic structure properties has been used to report on the effect of
the sulfur impurities on the behavior of the liquid. Although pure sulfur is
known to form chains in the liquid phase, we have not found any tendency
towards polymerization in our liquid simulation. Rather, a net S-S repulsion is
evident, and we propose an explanation for this effect in terms of the
electronic structure. The inspection of the dynamical properties of the system
suggests that the sulfur impurities have a negligible effect on the viscosity
of Earth's liquid core.Comment: 24 pages (including 8 figures
The Influence of Disorder on Thermotropic Nematic Liquid Crystals Phase Behavior
We review the theoretical research on the influence of disorder on structure and phase behavior of condensed matter system exhibiting continuous symmetry breaking focusing on liquid crystal phase transitions. We discuss the main properties of liquid crystals as adequate systems in which several open questions with respect to the impact of disorder on universal phase and structural behavior could be explored. Main advantages of liquid crystalline materials and different experimental realizations of random field-type disorder imposed on liquid crystal phases are described
Chemical Analysis of the Brightest Star of the Cetus II Ultra-Faint Dwarf Galaxy Candidate
We present a detailed chemical abundance analysis of the brightest star in
the ultra-faint dwarf (UFD) galaxy candidate Cetus II from high-resolution
Magellan/MIKE spectra. For this star, DES J011740.53-173053, abundances or
upper limits of 18 elements from Carbon to Europium are derived. Its chemical
abundances generally follow those of other UFD galaxy stars, with a slight
enhancement of the alpha-elements (Mg, Si, and Ca) and low neutron-capture
element (Sr, Ba, Eu) abundances supporting the classification of Cetus II as a
likely UFD. The star exhibits lower Sc, Ti, and V abundances than Milky Way
(MW) halo stars with similar metallicity. This signature is consistent with
yields from a supernova (SN) originating from a star with a mass of ~11.2 solar
masses. In addition, the star has a Potassium abundance of [K/Fe] = 0.81 which
is somewhat higher than the K abundances of MW halo stars with similar
metallicity, a signature which is also present in a number of UFD galaxies. A
comparison including globular clusters (GC) and stellar stream stars suggests
that high K is a specific characteristic for some UFD galaxy stars and can thus
be used to help classify objects as UFD galaxies.Comment: 15 pages, 7 figures, 5 tables, accepted to Ap
Modeling Insertional Mutagenesis Using Gene Length and Expression in Murine Embryonic Stem Cells
Background. High-throughput mutagenesis of the mammalian genome is a powerful means to facilitate analysis of gene function. Gene trapping in embryonic stem cells (ESCs) is the most widely used form of insertional mutagenesis in mammals. However, the rules governing its efficiency are not fully understood, and the effects of vector design on the likelihood of genetrapping events have not been tested on a genome-wide scale. Methodology/Principal Findings. In this study, we used public gene-trap data to model gene-trap likelihood. Using the association of gene length and gene expression with gene-trap likelihood, we constructed spline-based regression models that characterize which genes are susceptible and which genes are resistant to gene-trapping techniques. We report results for three classes of gene-trap vectors, showing that both length and expression are significant determinants of trap likelihood for all vectors. Using our models, we also quantitatively identifie
Prognostic significance of microvessel density and other variables in Japanese and British patients with primary invasive breast cancer
The purpose of this study is to investigate the associations of microvessel density (MVD) and other pathological variables with survival, and whether they accounted for survival differences between Japanese and British patients. One hundred seventy-three Japanese and 184 British patients were included in the study. British patients were significantly older (56.3±11.4 years vs 52.5±12.9 years; P<0.01) and had smaller tumours (2.2±1.3 vs 2.7±1.8 cm; P<0.01), which were more frequently oestrogen receptor positive (78.8 vs 57.2%, P<0.01), had more grade III tumours (29.9 vs 21.4%, P=0.04) and more infiltrating lobular carcinomas (13.6 vs 4.0%, P<0.01) and a higher MVD compared with Japanese patients (57.9±19.8 vs 53.2±18.6; P=0.01). However, no difference in the prevalence of lymph-node metastasis was found between them (39.1 vs 37.5%, P=0.75). Younger British patients (age <50 years) had the highest MVD compared with Japanese and older British patients (P<0.01). Japanese patients were proportionately more likely to receive chemotherapy than endocrine therapy (P<0.01). British patients had a significantly worse relapse-free survival and overall survival compared with Japanese patients, after statistical adjustment for variables (hazard ratio=2.1, 2.4, P<0.01, P<0.01, respectively), especially, in T2 stage, low MVD and older subgroup (HR: 3.6, 5.0; 3.1, 3.3; 3.2, 3.9, respectively), but only in ER negative cases (P=0.04, P=0.01, respectively). The present study shows that MVD contributes to the Japanese–British disparity in breast cancer. However, the MVD variability did not explain the survival differences between Japanese and British patients
The mammalian gene function resource: the International Knockout Mouse Consortium.
In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action. Now, 5 years later, the IKMC members have developed high-throughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones and more than 1,700 mutant mouse strains, most of them conditional. A common IKMC web portal (www.knockoutmouse.org) has been established, allowing easy access to this unparalleled biological resource. The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research
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