A NEAT Way to do Network Programming

This work has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 644334 (NEAT). The views expressed are solely those of the author(s)Peer reviewedPreprin

De-ossifying the Internet Transport Layer : A Survey and Future Perspectives

ACKNOWLEDGMENT The authors would like to thank the anonymous reviewers for their useful suggestions and comments.Peer reviewedPublisher PD

Myocardial recovery in peri-partum cardiomyopathy after continuous flow left ventricular assist device

Left ventricular assist devices (LVADs) offer effective therapy for severe heart failure (HF) as bridge to transplantation or destination therapy. Rarely, the sustained unloading provided by the LVAD has led to cardiac reverse remodelling and recovery, permitting explantation of the device. We describe the clinical course of a patient with severe peri-partum cardiomyopathy (PPCM) rescued with a continuous flow LVAD, who experienced recovery and explantation. We discuss assessment of and criteria for recovery

Theoretical Analysis of an Ideal Startup Scheme in Multihomed SCTP

Abstract. SCTP congestion control includes the slow start mechanism to probe the network for available bandwidth. In case of path swap in a multihomed association, this mechanism may cause a sudden drop in throughput and increased message delays. By estimating the available bandwidth on the alternate path it is possible to utilize a more efficient startup scheme. In this paper, we analytically compare and quantify the degrading impact of slow start in relation to an ideal startup scenario. We identify three different scenarios, where a path swap could occur. Further, we identify relevant traffic for these scenarios. Our results point out that the most prominent performance gain is seen for applications generating high traffic loads, like video-conferencing. Moreover, the results show an increasing impact of an improved startup mechanism with increasing RTTs

Low-Latency Scheduling in MPTCP

The demand for mobile communication is continuously increasing, and mobile devices are now the communication device of choice for many people. To guarantee connectivity and performance, mobile devices are typically equipped with multiple interfaces. To this end, exploiting multiple available interfaces is also a crucial aspect of the upcoming 5G standard for reducing costs, easing network management, and providing a good user experience. Multi-path protocols, such as multi-path TCP (MPTCP), can be used to provide performance optimization through load-balancing and resilience to coverage drops and link failures, however, they do not automatically guarantee better performance. For instance, low-latency communication has been proven hard to achieve when a device has network interfaces with asymmetric capacity and delay (e.g., LTE and WLAN). For multi-path communication, the data scheduler is vital to provide low latency, since it decides over which network interface to send individual data segments. In this paper, we focus on the MPTCP scheduler with the goal of providing a good user experience for latency-sensitive applications when interface quality is asymmetric. After an initial assessment of existing scheduling algorithms, we present two novel scheduling techniques: the block estimation (BLEST) scheduler and the shortest transmission time first (STTF) scheduler. BLEST and STTF are compared with existing schedulers in both emulated and real-world environments and are shown to reduce web object transmission times with up to 51% and provide 45% faster communication for interactive applications, compared with MPTCP's default scheduler

Diversity of respiratory parameters and metabolic adaptation to low oxygen tension in mesenchymal stromal cells

Objective Cell metabolism has been shown to play an active role in regulation of stemness and fate decision. In order to identify favorable culture conditions for mesenchymal stromal cells (MSCs) prior to transplantation, this study aimed to characterize the metabolic function of MSCs from different developmental stages in response to different oxygen tension during expansion. Materials and methods We cultured human fetal cardiac MSCs and human adult bone-marrow MSCs for a week under hypoxia (3% O2) and normoxia (20% O2). We performed mitochondrial characterization and assessed oxygen consumption- and extracellular acidification-rates (OCR and ECAR) in addition to oxygen-sensitive respiration and mitochondrial complex activities, using both the Seahorse and Oroboros systems. Results Adult and fetal MSCs displayed similar basal respiration and mitochondrial amount, however fetal MSCs had lower spare respiratory capacity and apparent coupling efficiency. Fetal MSCs expanded in either hypoxia or normoxia demonstrated similar acidification rates, while adult MSCs downregulated their aerobic glycolysis in normoxia. Acute decrease in oxygen tension caused a higher respiratory inhibition in adult compared to fetal MSCs. In both sources of MSCs, minor changes in complex activities in normoxic and hypoxic cultures were found. Conclusions In contrast to adult MSCs, fetal MSCs displayed similar respiration and aerobic glycolysis at different O2 culture concentrations during expansion. Adult MSCs adjusted their respiration to glycolytic activities, depending on the culture conditions thus displaying a more mature metabolic function. These findings are relevant for establishing optimal in vitro culturing conditions, with the aim to maximize engraftment and therapeutic outcome.CC BY-NC-ND 4.0Corresponding author: Department of Surgical Sciences, Uppsala University, 751 85, Uppsala, Sweden. E-mail address: [email protected] (K.-H. Grinnemo).Available online 3 February 2022, Version of Record 5 February 2022The project was funded by Karolinska Institute-Mayo Clinic Collaborative Grant 2013; The Swedish Research Council young investigator: 2013–3590; Stockholm county; The Swedish Research Council; The Family Erling-Persson Foundation; ERC-2018-AdG (834860 EYELETS); Uppsala county; Uppsala County Association against Heart and Lung Diseases; and Higher Education of the Russian Federation (agreement no. 075-15-2020-899).</p

An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation

To characterize molecular changes during cell type transitions, the authors develop a method to simultaneously measure protein expression and thermal stability changes. They apply this approach to study differences between human pluripotent stem cells, their progenies, parental and allogeneic cells. Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro

NEAT : A Platform- And Protocol-Independent Internet Transport API

ACKNOWLEDGMENT The authors would like to thank the anonymous reviewers for their useful remarks. This work has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 644334 (NEAT). The views expressed are solely those of the authors.Peer reviewedPostprin

Human Pluripotent Stem Cells Differentiated in Fully Defined Medium Generate Hematopoietic CD34+ and CD34− Progenitors with Distinct Characteristics

Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In particular, the use of a fully defined medium (FDM) would avoid biases induced by unidentified factors contained in serum, and would also allow key molecular mediators involved in such a process to be identified. Our goal was to induce in vitro, the differentiation of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) into morphologically and phenotypically mature leukocytes and erythrocytes, in the complete absence of serum and feeder cells

The Significance of Cell-related Challenges in the Clinical Application of Tissue Engineering.

Tissue engineering is increasingly being recognized as a new approach that could alleviate the burden of tissue damage currently managed with transplants or synthetic devices. Making this novel approach available in the future for patients who would potentially benefit is largely dependent on understanding and addressing all those factors that impede the translation of this technology to the clinic. Cell-associated factors in particular raise many challenges, including those related to cell sources, up- and downstream techniques, preservation, and the creation of in vitro microenvironments that enable cells to grow and function as far as possible as they would in vivo. This paper highlights the main confounding issues associated with cells in tissue engineering and how these issues may hinder the advancement of therapeutic tissue engineering. This article is protected by copyright. All rights reserved