Extended flow cytometry characterization of normal bone marrow progenitor cells by simultaneous detection of aldehyde dehydrogenase and early hematopoietic antigens: implication for erythroid differentiation studies

<p>Abstract</p> <p>Background</p> <p>Aldehyde dehydrogenase (ALDH) is a cytosolic enzyme highly expressed in hematopoietic precursors from cord blood and granulocyte-colony stimulating factor mobilized peripheral blood, as well as in bone marrow from patients with acute myeloblastic leukemia. As regards human normal bone marrow, detailed characterization of ALDH⁺cells has been addressed by one single study (Gentry <it>et al</it>, 2007). The goal of our work was to provide new information about the dissection of normal bone marrow progenitor cells based upon the simultaneous detection by flow cytometry of ALDH and early hematopoietic antigens, with particular attention to the expression of ALDH on erythroid precursors. To this aim, we used three kinds of approach: i) multidimensional analytical flow cytometry, detecting ALDH and early hematopoietic antigens in normal bone marrow; ii) fluorescence activated cell sorting of distinct subpopulations of progenitor cells, followed by <it>in vitro </it>induction of erythroid differentiation; iii) detection of ALDH⁺cellular subsets in bone marrow from pure red cell aplasia patients.</p> <p>Results</p> <p>In normal bone marrow, we identified three populations of cells, namely ALDH⁺CD34⁺, ALDH^-CD34⁺and ALDH⁺CD34^-(median percentages were 0.52, 0.53 and 0.57, respectively). As compared to ALDH^-CD34⁺cells, ALDH⁺CD34⁺cells expressed the phenotypic profile of primitive hematopoietic progenitor cells, with brighter expression of CD117 and CD133, accompanied by lower display of CD38 and CD45RA. Of interest, ALDH⁺CD34^-population disclosed a straightforward erythroid commitment, on the basis of three orders of evidences. First of all, ALDH⁺CD34^-cells showed a CD71^bright, CD105⁺, CD45^-phenotype. Secondly, induction of differentiation experiments evidenced a clear-cut expression of glycophorin A (CD235a). Finally, ALDH⁺CD34^-precursors were not detectable in patients with pure red cell aplasia (PRCA).</p> <p>Conclusion</p> <p>Our study, comparing surface antigen expression of ALDH⁺/CD34⁺, ALDH^-/CD34⁺and ALDH⁺/CD34^-progenitor cell subsets in human bone marrow, clearly indicated that ALDH⁺CD34^-cells are mainly committed towards erythropoiesis. To the best of our knowledge this finding is new and could be useful for basic studies about normal erythropoietic differentiation as well as for enabling the employment of ALDH as a red cell marker in polychromatic flow cytometry characterization of bone marrow from patients with aplastic anemia and myelodysplasia.</p

A Case Series of Blastic Plasmacytoid Dendritic Cell Neoplasia

Blastic plasmacytoid dendritic cell neoplasm (BPDCN), an extremely rare and aggressive tumor, derives from plasmacytoid dendritic cell precursors and is characterized by CD4 and CD56 positivity accompanied by the expression of isolated myeloid, B- or T-cell lineage markers. Despite the recent introduction of specific targeted therapies, prognosis is still poor with a median overall survival of one year, and allogeneic bone marrow transplantation remains the only curative treatment in eligible patients. In this series, we described two cases of adult BPDCN treated with high dose cytarabine and methotrexate and autologous hematopoietic stem cell transplantation, or fludarabine, cytarabine, and idarubicin achieving the first a complete lasting remission, while the second only a transient improvement in skin lesion

Social and Non-social Brain Areas in Risk Behaviour: The Role of Social Context

This work was supported by the Spanish Ministry of Economy, Industry, and Competitiveness through a grant awarded (PSI2016-80558-R to A.Catena) and a postdoctoral contract of the University of Granada (to S. Baltruschat) .The human brain contains social areas that become active when interacting with another human. These are located in the ventral prefrontal and mediodorsal cortices, adjacent to areas involved in reward processing and cognitive control. Human behaviour is strongly influenced by the social context. This is particularly evident when observing greater risk propensity in the presence of a peer, particularly during adolescence and emerging adulthood. We explored the widely held view that enhanced risk propensity is the consequence of weak cognitive control. We used brain activity, estimated from EEG recordings in a sample of 114 emerging adult dyads whilst performing a risk perception task, to predict risk behaviour in a subsequent driving simulation task. Being with a peer reduced the ability to discriminate riskiness in images of traffic scenes, biased responses towards the perception of no-risk, and increased the rate of accidents in the driving simulation. Risk perception involved three sets of clusters showing activity only when being with a peer, only when being alone, and in both social contexts. Functional connectivity between the clusters accounted for the later driving simulation performance depending on the peer’s presence. In the light of our findings, greater risk-taking, when a peer is present, seems to be triggered by the activation of a different, less efficient brain network for risk-processing.Spanish Ministry of Economy, Industry, and Competitiveness PSI2016-80558-RUniversity of Granad

Adolescents' involvement in cyber bullying and perceptions of school: the importance of perceived peer acceptance for female adolescents

Young people are spending increasing amounts of time using digital technology and, as such, are at great risk of being involved in cyber bullying as a victim, bully, or bully/victim. Despite cyber bullying typically occurring outside the school environment, the impact of being involved in cyber bullying is likely to spill over to school. Fully 285 11- to 15-year-olds (125 male and 160 female, M age = 12.19 years, SD = 1.03) completed measures of cyber bullying involvement, self-esteem, trust, perceived peer acceptance, and perceptions of the value of learning and the importance of school. For young women, involvement in cyber bullying as a victim, bully, or bully/victim negatively predicted perceptions of learning and school, and perceived peer acceptance mediated this relationship. The results indicated that involvement in cyber bullying negatively predicted perceived peer acceptance which, in turn, positively predicted perceptions of learning and school. For young men, fulfilling the bully/victim role negatively predicted perceptions of learning and school. Consequently, for young women in particular, involvement in cyber bullying spills over to impact perceptions of learning. The findings of the current study highlight how stressors external to the school environment can adversely impact young women's perceptions of school and also have implications for the development of interventions designed to ameliorate the effects of cyber bullying

Core belief content examined in a large sample of patients using online cognitive behaviour therapy

© 2015 The Authors. Abstract Background Computerised cognitive behavioural therapy provides a unique opportunity to collect and analyse data regarding the idiosyncratic content of people's core beliefs about the self, others and the world. Methods 'Beating the Blues' users recorded a core belief derived through the downward arrow technique. Core beliefs from 1813 mental health patients were coded into 10 categories. Results The most common were global self-evaluation, attachment, and competence. Women were more likely, and men were less likely (than chance), to provide an attachment-related core belief; and men were more likely, and women less likely, to provide a self-competence-related core belief. This may be linked to gender differences in sources of self-esteem. Those who were suffering from anxiety were more likely to provide power- and control-themed core beliefs and less likely to provide attachment core beliefs than chance. Finally, those who had thoughts of suicide in the preceding week reported less competence themed core beliefs and more global self-evaluation (e.g., 'I am useless') core beliefs than chance. Limitations Concurrent symptom level was not available. The sample was not nationally representative, and featured programme completers only. Conclusions Men and women may focus on different core beliefs in the context of CBT. Those suffering anxiety may need a therapeutic focus on power and control. A complete rejection of the self (not just within one domain, such as competence) may be linked to thoughts of suicide. Future research should examine how individual differences and symptom severity influence core beliefs

The effect of parental style on bullying and cyber bullying behaviors and the mediating role of peer attachment relationships: A longitudinal study

The purpose of the present study was the examination of the longitudinal effect of parental style on short-term changes in conventional and cyber forms of bullying/victimization, and the investigation of the mediating role of peer attachment relationships on this effect. The participants were 861 children and adolescents (52% girls, M  = 11.72 years) attending Cyprus public institutions. Students provided information during three measurement points. There was a six and a 12 week interval among the three measurement points, respectively. The findings of the study indicated that parenting seems to be a significant predictor of all forms of bullying/victimization, conventional and cyber, in early adolescents, even when accounting for bullying/victimization levels eighteen weeks back. More importantly, results showed that the effect of parental style on bullying forms was mediated by peer attachment relationships. Results are discussed in the light of theoretical and practical implications. [Abstract copyright: Copyright © 2018 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

DECISIONE ED EMOZIONE. LA REGOLAZIONE EMOZIONALE NELLA VALUTAZIONE DELLE ALTERNATIVE

La varietà e complessità delle ricerche sul rapporto fra l’emozione e la decisione, rapporto indagato da discipline diverse (psicologia, economia, neuroscienze e così via) che trattano questo problema avvalendosi di propri metodi, necessita di uno sforzo di sistematizzazione dei risultati raggiunti sinora e un’individuazione delle questioni ancora aperte. Nel loro insieme, i dati raccolti da numerosi ricercatori nel campo della neuropsicologia e delle neuroscienze mostrano come i processi decisionali umani siano tutt’altro che indifferenti ai vissuti emozionali a essi contingenti. Anzi, il processo decisionale sembra dipendere in molti e importanti modi dai substrati neurali che regolano le emozioni e i sentimenti. In quest’ottica, dunque, si può dire che in determinate condizioni siano proprio le emozioni a guidare i meccanismi valutativi che intervengono nel processo decisionale. Uno dei compiti della ricerca futura, come evidenziato da un crescente numero di ricercatori, consisterà nell’integrare gli effetti motivazionali e informazionali delle emozioni per comprenderne pienamente il ruolo e le funzioni che esse ricoprono nella presa di decisione. Infatti, perlopiù le motivazioni (in termini di interessi, obiettivi, scopi, desideri, aspettative) vengono chiamate in causa soltanto per spiegare post hoc i risultati trovati, mentre occorrerebbe inserirle nel disegno sperimentale sin dall’inizio. La regolazione emozionale, con il suo potere motivazionale, consente di tener conto di questo aspetto non marginale del problema. Il tentativo di integrare gli aspetti valutativi e quelli regolativi nello studio della relazione tra emozione e decision making, operazione ancora allo stato di work in progress, appare potenzialmente in grado di apportare nuovi e originali contributi teorici e metodologici